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1151 - 1200 of 1570 citations since 2019

• JNK signaling controls branching, nucleokinesis, and positioning of centrosomes and primary cilia in migrating cortical interneurons

  Skye E. Smith, Nicholas K. Coker, Eric S. Tucker, bioRxiv - Neuroscience 2020

  Quote: Cultures were treated with pre-warmed 37°C serum-free media containing a 1:1000 dilution of DMSO for vehicle control or 20 μM SP600125 pan-JNK inhibitor (Enzo Life Sciences BML-EI305-0010; Farmingdale, NY, USA) and immediately transferred to a Zeiss 710 Confocal Microscope with stable environmental controls maintained at 37°C with 5% humidified CO2 ...
• Neural stem cells traffic functional mitochondria via extracellular vesicles to correct mitochondrial dysfunction in target cells

  Luca Peruzzotti-Jametti, et al., bioRxiv - Neuroscience 2020

  Quote: ... Germany) at 50,000 cells/well and the next day stimulated with 50 ng/ml LPS (Enzo life sciences). After 1 hr of LPS stimulation ...
• Neural stem cells traffic functional mitochondria via extracellular vesicles to correct mitochondrial dysfunction in target cells

  Luca Peruzzotti-Jametti, et al., bioRxiv - Neuroscience 2020

  Quote: ... let rest overnight and stimulated with 50 ng/ml LPS (Enzo life sciences). After 1 hr from LPS stimulation ...
• Neural stem cells traffic functional mitochondria via extracellular vesicles to correct mitochondrial dysfunction in target cells

  Luca Peruzzotti-Jametti, et al., bioRxiv - Neuroscience 2020

  Quote: ... Mφ were then stimulated with 50 ng/ml LPS (Enzo life sciences) and after 1 hr ...
• Neural stem cells traffic functional mitochondria via extracellular vesicles to correct mitochondrial dysfunction in target cells

  Luca Peruzzotti-Jametti, et al., bioRxiv - Neuroscience 2020

  Quote: ... Mφ were stimulated by adding 50 ng/ml LPS (Enzo life sciences). After 1h from LPS stimulation ...
• Neural stem cells traffic functional mitochondria via extracellular vesicles to correct mitochondrial dysfunction in target cells

  Luca Peruzzotti-Jametti, et al., bioRxiv - Neuroscience 2020

  Quote: ... The cells were let to rest overnight and stimulated with 50 ng/ml lipopolysaccharide (LPS) (Enzo life sciences). After 30 min from the start of the stimulation ...
• Gene replacement therapy provides benefit in an adult mouse model of Leigh syndrome

  Robin Reynaud Dulaurier, et al., bioRxiv - Neuroscience 2020

  Quote: ... Calnexin (Rabbit, 1:5000, Enzo Life Science, ADI-SPA-860). After washing for 30 minutes in TBST ...
• Designed Variants of ACE2-Fc that Decouple Anti-SARS-CoV-2 Activities from Unwanted Cardiovascular Effects

  Pan Liu, Xinfang Xie, Li Gao, Jing Jin, bioRxiv - Biochemistry 2020

  Quote: ACE2-Fc peptidase activity assay using surrogate fluorogenic substrate Mca-APK(Dnp) (Enzo Life Sciences, Farmingdale, NY) was performed in black microtiter plates ...
• Alternative catalytic mechanisms driven by structural plasticity is an emerging theme in HAS-GTPases, Era and FeoB

  Sahil Batra, Ashok Kumar, Balaji Prakash, bioRxiv - Biochemistry 2020

  Quote: ... The reactions were quantified through Biomol Green reagent (Enzo Life Sciences), a malachite green-based phosphate detection system ...
• Evolutionarily conserved chaperone-mediated proteasomal degradation of a disease-linked aspartoacylase variant

  Sarah K. Gersing, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-20S α-subunits (Enzo Life Sciences, MCP231 PW8195), anti-Hsp70 (Invitrogen ...
• Evolutionarily conserved chaperone-mediated proteasomal degradation of a disease-linked aspartoacylase variant

  Sarah K. Gersing, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-Rpn1 (Enzo Life Sciences, p112-1 PW9270), anti-20S α-subunits (Enzo Life Sciences ...
• BAG3 Pro209 mutants associated with myopathy and neuropathy relocate chaperones of the CASA-complex to aggresomes

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit polyclonal anti-Cu/Zn superoxide dismutase SOD1 (SOD-100, Enzo Life Sciences, Farmingdale, NY, USA), anti-ubiquitin (3936 ...
• DET1-mediated COP1 regulation avoids HY5 activity over second-site targets to tune plant photomorphogenesis

  Esther Cañibano, et al., bioRxiv - Plant Biology 2020

  Quote: ... anti-RPT5 (Enzo Life Sciences# BML-PW8245); anti-HY5 (Agrisera #AS121867 or Abiocode #R1245-1b) ...
• Direct phosphorylation of HY5 by SPA1 kinase to regulate photomorphogenesis in Arabidopsis

  Wenli Wang, et al., bioRxiv - Plant Biology 2020

  Quote: ... anti-Tubulin antibodies (Enzo Life Sciences, Cat. # BML-PW8770-0025). Secondary HRP bound antibodies were visualized with Super Signal West Pico Chemiluminescent substrate (Pierce Biotechnology Inc. ...
• Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

  Ashwin Nair, et al., bioRxiv - Plant Biology 2020

  Quote: ... and SUMO domain mutants of βC1 were incubated with SUMOylation cascade enzymes as per manufacturer’s instructions (SUMOylation kit, ENZO life sciences). HsSUMO1 was replaced with NbSUMO1 ...
• Inhibition of HIF-1α accumulation in prostate cancer cells is initiated during early stages of mammalian orthoreovirus infection

  Luke D. Bussiere, Cathy L. Miller, bioRxiv - Microbiology 2020

  Quote: ... MG132 (Enzo Life Sciences) was used at a concentration of 50 μM ...
• Inhibition of HIF-1α accumulation in prostate cancer cells is initiated during early stages of mammalian orthoreovirus infection

  Luke D. Bussiere, Cathy L. Miller, bioRxiv - Microbiology 2020

  Quote: ... Z-VAD-FMK (Enzo Life Sciences) at a concentration of 20 μM ...
• Endogenous Rab29 does not impact basal or nigericin and monensin stimulated LRRK2 pathway activity

  Alexia F. Kalogeropulou, et al., bioRxiv - Neuroscience 2020

  Quote: ... Microcystin-LR was purchased from Enzo Life Sciences (ALX-350-012), oriole fluorescent gel stain was purchased from Bio-Rad (#161-0495) ...
• No change in oxytocin level before a human intergroup competition: study among Polish players before rugby and handball matches

  Slawomir Koziel, et al., bioRxiv - Evolutionary Biology 2020

  Quote: ... Creatinine (Cr) concentration was measured with the Creatinine Colorimetric Detection Kit (Catalog #: ADI-907-030A; Enzo Life Science, USA). Both analyses were conducted according to the assay procedures described in provided manuals ...
• No change in oxytocin level before a human intergroup competition: study among Polish players before rugby and handball matches

  Slawomir Koziel, et al., bioRxiv - Evolutionary Biology 2020

  Quote: ... Oxytocin concentration was estimated by ELISA immunoassay (Catalog #: ADI-901-153A; Enzo Life Science, USA). Creatinine (Cr ...
• Ablation of STAT3 in Purkinje Cells Reorganizes Cerebellar Synaptic Plasticity in Long-Term Fear Memory Network

  Jeong-Kyu Han, et al., bioRxiv - Neuroscience 2020

  Quote: ... and goat anti-rabbit IgG (1:10,000, Enzo Life Science, NY, USA). The membranes were visualized using an ECL detection kit (SurModics ...
• MT5-MMP controls APP and β-CTF/C99 metabolism through proteolytic-dependent and -independent mechanisms relevant for Alzheimer’s disease

  Laura García-González, et al., bioRxiv - Neuroscience 2020

  Quote: ... The proteasome inhibitor MG132 was used at 5 μM (Enzo Life Science, Lyon, France) and bafilomycin A1 (Sigma-Aldrich ...
• Gene expression and chromatin accessibility during progressive EMT and MET linked to dynamic CTCF engagement

  Kelsey S. Johnson, et al., bioRxiv - Cell Biology 2020

  Quote: ... and 100 ng/mL cholera toxin (Enzo Life Sciences). Cells were plated at 10,000 cells/cm2 and passaged every other day to maintain consistent densities ...
• Functional Evidence for Biased Inhibition of G protein Signaling by YM-254890 in Human Coronary Artery Endothelial Cells

  Qianman Peng, et al., bioRxiv - Pharmacology and Toxicology 2020

  Quote: Intra-cellular cAMP levels were determined by using the Direct cAMP Elisa kit (Enzo Life Sciences) as previously reported (16) ...
• Functional Evidence for Biased Inhibition of G protein Signaling by YM-254890 in Human Coronary Artery Endothelial Cells

  Qianman Peng, et al., bioRxiv - Pharmacology and Toxicology 2020

  Quote: ... the original medium was removed and the assay medium from FluoForteTM kit (Enzo Life Sciences) containing the Ca2+ dye was added and receptor-mediated Ca2+ mobilization was determined as previously described (13) ...
• Reactive Oxygen Species Modulate Activity-Dependent AMPA Receptor Transport in C. elegans

  Rachel L. Doser, et al., bioRxiv - Neuroscience 2020

  Quote: A 0.5 mM stock of nemadipine (Enzo Life Sciences) was dissolved in 0.1% DMSO and the concentration was adjusted to 10 μM (a concentration reported to decrease calcium influx in exposed neurons in vivo by ∼70% ...
• Oxytocin has sex-specific effects on social behaviour and hypothalamic oxytocin immunoreactive cells but not hippocampal neurogenesis in adult rats

  Paula Duarte-Guterman, et al., bioRxiv - Neuroscience 2020

  Quote: ... Hypothalamus homogenates were run in duplicate on a modified ELISA using the lysis buffer to prepare the standard curve (Enzo Life Sciences). Oxytocin levels were normalized to protein content using a BCA protein assay (ThermoFisher ...
• Oxytocin has sex-specific effects on social behaviour and hypothalamic oxytocin immunoreactive cells but not hippocampal neurogenesis in adult rats

  Paula Duarte-Guterman, et al., bioRxiv - Neuroscience 2020

  Quote: Oxytocin concentrations were analyzed using an enzyme-linked immunosorbent assay kit (Enzo Life Sciences, ADI-900-153A) on plasma and hypothalamus homogenate samples ...
• The small GTPase Arf6 functions as a membrane tether in a chemically-defined reconstitution system

  Kana Fujibayashi, Joji Mima, bioRxiv - Cell Biology 2020

  Quote: ... using the Malachite Green-based reagent Biomol Green (Enzo Life Sciences) as described for the activity assays with Rab-family small GTPases (Inoshita and Mima ...
• The small GTPase Arf6 functions as a membrane tether in a chemically-defined reconstitution system

  Kana Fujibayashi, Joji Mima, bioRxiv - Cell Biology 2020

  Quote: ... Concentrations of phosphate molecules released in the reactions were calculated using the corrected A620 data and the A620 values of phosphate standards (Enzo Life Sciences). Means and standard deviations of the specific GTPase activities (μM phosphate/min/μM protein ...
• The Pharmacodynamic-Toxicodynamic Relationship of AUC and CMAX in Vancomycin Induced Kidney Injury in an Animal Model

  Sean N. Avedissian, et al., bioRxiv - Pharmacology and Toxicology 2020

  Quote: ... United States Pharmacopeia was used (Enzo Life Science, Farmingdale, NY) with a purity of 99.3% ...
• The 20S proteasome activator PA28γ controls the compaction of chromatin

  Didier Fesquet, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-α4 (1:2000; mouse monoclonal BML-PW8120, ENZO Life Sciences); anti-PA28γ (mouse monoclonal ...
• The 20S proteasome activator PA28γ controls the compaction of chromatin

  Didier Fesquet, et al., bioRxiv - Cell Biology 2020

  Quote: The following antibodies were used at 1:1000 dilution for immunoblotting and 1-3 μg/ml for immunoprecipitation: anti-PA28γ (rabbit polyclonal BML-PW8190, ENZO Life Sciences), anti-α4 (1:2000 ...
• Autophagy in T cells from aged donors is maintained by spermidine, and correlates with function and vaccine responses

  Ghada Alsaleh, et al., bioRxiv - Physiology 2020

  Quote: ... or 1 mM difluoromethylornithine (DFMO, Enzo Life Sciences) or 10 μM GC7 (or as indicated ...
• Regulated assembly of the ER membrane protein complex

  Tino Pleiner, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Hsp70 (ADI-SPA-812, Enzo Life Sciences, USA); BiP (610978 ...
• Illuminating host-mycobacterial interactions with functional genomic screening to inhibit mycobacterial pathogenesis

  Yong Lai, et al., bioRxiv - Microbiology 2020

  Quote: ... vinculin monoclonal antibody (Enzo life sciences), and goat anti-mouse IgG HRP conjugate (Thermo Scientific) ...
• Colonization with the commensal fungus Candida albicans perturbs the gut-brain axis through dysregulation of endocannabinoid signaling

  Laura Markey, et al., bioRxiv - Microbiology 2020

  Quote: ... Serum corticosterone was measured using a Corticosterone ELISA Kit (Enzo Life Sciences) following the manufacturer's small volume protocol ...
• BK polyomavirus evades innate immune sensing by disrupting the mitochondrial network and membrane potential and by promoting mitophagy

  Julia Manzetti, et al., bioRxiv - Microbiology 2020

  Quote: ... mouse monoclonal IgG1 anti-PDI (SPA-891, Enzo Life Science), polyclonal chicken anti-Calreticulin (ab14234 ...
• BK polyomavirus evades innate immune sensing by disrupting the mitochondrial network and membrane potential and by promoting mitophagy

  Julia Manzetti, et al., bioRxiv - Microbiology 2020

  Quote: ... and E64d (BML-PI107, Enzo Life Science) were diluted to generate a 10 mg/mL stock concentration in DMSO and Ethanol ...
• BK polyomavirus evades innate immune sensing by disrupting the mitochondrial network and membrane potential and by promoting mitophagy

  Julia Manzetti, et al., bioRxiv - Microbiology 2020

  Quote: ... The protease inhibitors pepstatin A1 (ALX-260-085, Enzo Life Science) and E64d (BML-PI107 ...
• Combination of antifungal drugs and protease inhibitors prevent Candida albicans biofilm formation and disrupt mature biofilms

  Matthew B. Lohse, et al., bioRxiv - Microbiology 2020

  Quote: The 53 member SCREEN-WELL® Protease Inhibitor Library (http://www.enzolifesciences.com/BML-2833/screen-well-protease-inhibitor-library/) was purchased from Enzo Life Sciences. The two aspartyl protease inhibitor libraries (from which we focused on nine FDA-approved HIV-1 protease inhibitors ...
• Disassembly and degradation of MinD oscillator complexes by Escherichia coli ClpXP

  Christopher J. LaBreck, et al., bioRxiv - Microbiology 2020

  Quote: ... Phosphate released in reactions was detected using Biomol green (Enzo Life Sciences) and compared to a phosphate standard curve.
• Mono-ADP-ribosylation by ARTD10 restricts Chikungunya virus replication by interfering with the proteolytic activity of nsP2

  Sarah Krieg, et al., bioRxiv - Microbiology 2020

  Quote: ... or 200 nM Bafilomycin A1 (Baf.A1) (Enzo Life Sciences) for 6 h ...
• Evaluation of 19 antiviral drugs against SARS-CoV-2 Infection

  Shufeng Liu, et al., bioRxiv - Microbiology 2020

  Quote: ... Favilavir was obtained from Cayman Chemical and chloromethylketone was from Enzo Life Sciences.
• Conserved regulation of omptin proteases by the PhoPQ two-component regulatory system in Enterobacteriaceae

  Youn Hee Cho, et al., bioRxiv - Microbiology 2020

  Quote: Blocked blots were washed three times in 1 × TBST washes before incubation with pre-chilled mouse anti-DnaK antibody (1:10,000 Ab in 3 % BSA and 0.01 % sodium azide in TBST; Enzo Life Sciences, Inc.) for one hour ...
• Hsp70 chaperone blocks α-synuclein oligomer formation via a novel engagement mechanism

  Jiahui Tao, et al., bioRxiv - Neuroscience 2020

  Quote: ... and mouse monoclonal antibody G3.1 anti Hsp27 antibody (Enzo Life Science, Inc., Farmingdale, NY) were diluted at 1/1000 ...
• Hsp70 chaperone blocks α-synuclein oligomer formation via a novel engagement mechanism

  Jiahui Tao, et al., bioRxiv - Neuroscience 2020

  Quote: ... rabbit anti Hsp70 antibody (Enzo Life Science, Inc., Farmingdale, NY, ADI-SPA-812) and mouse monoclonal antibody G3.1 anti Hsp27 antibody (Enzo Life Science ...
• The tumor suppressor protein PTEN undergoes amyloid-like aggregation in tumor cells

  Filip Claes, et al., bioRxiv - Cancer Biology 2020

  Quote: ... rabbit polyclonal anti-p62 (Enzo Life Sciences) and rabbit polyclonal anti-Osgin-1 (Bioss Antibodies) ...
• Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death

  Nina Meyer, et al., bioRxiv - Cancer Biology 2020

  Quote: ... loperamide hydrochloride (LOP; Enzo Life Science, ALX-550-253), pimozide (PIMO ...
• Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death

  Nina Meyer, et al., bioRxiv - Cancer Biology 2020

  Quote: ... staurosporine (STS; Enzo Life Science, 380-014-M001), AS 19 (Tocris Bioscience ...