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1051 - 1100 of 1570 citations since 2019

• Cdk1-dependent destabilization of long astral microtubules is required for spindle orientation

  Divya Singh, et al., bioRxiv - Cell Biology 2020

  Quote: ... were added at 500 nM and 2 μM respectively for 90 min while 100 nM of Plk1 inhibitor (BI 2536; Enzo Life Sciences) was added for one hour prior to imaging ...
• A non-canonical role for the autophagy machinery in anti-retroviral signaling mediated by TRIM5α

  Bhaskar Saha, Devon Chisholm, Michael A Mandell, bioRxiv - Cell Biology 2020

  Quote: ... NF-κB and AP1 inhibitors (BAY 11-7085 and SP600125) were purchased from Enzo Life Sciences. 3-methyladenine was purchased from Sigma.
• Cardiolipin targets a dynamin related protein to the nuclear membrane

  Usha Pallabi Kar, Himani Dey, Abdur Rahaman, bioRxiv - Cell Biology 2020

  Quote: The GTP hydrolysis activity of recombinant Drp6 and Drp6 I553M was measured in a colorimetric assay using Malachite Green-based phosphate assay reagent (BIOMOL Green, Enzo Life Sciences). The GTPase assay (20 ul in 25mM HEPES pH7.5 ...
• IL8 Drives an Elaborate Signal Transduction Process for CD38 to Produce NAADP from NAAD and NADP⁺ in Endolysosomes to Effect Cell Migration

  Tae-Sik Nam, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-goat or anti-mouse antibodies (1:5000; Enzo Life Sciences) in the blocking buffer at room temperature for 2 h ...
• Genetic interaction between profilin and myosin II reveals a potential role for myosin II in actin filament disassembly in vivo

  Paola Zambon, et al., bioRxiv - Cell Biology 2020

  Quote: ... with jasplakinolide (jasp; Enzo Life Sciences) by the addition of the drug to the isolated rings at the final concentration of 20 μM.
• Genetic interaction between profilin and myosin II reveals a potential role for myosin II in actin filament disassembly in vivo

  Paola Zambon, et al., bioRxiv - Cell Biology 2020

  Quote: YES agar plates in Figure 5A were prepared by the addition of different concentrations of latrunculin A (Lat-A; Enzo Life Sciences), where cells were successively being spotted at 10-fold serial dilutions ...
• A new form of actin assembly around the Shigella-containing vacuole regulates its intracellular niche

  Sonja Kühn, et al., bioRxiv - Cell Biology 2020

  Quote: ... 10 µM Wortmannin (Enzo Life Sciences Inc, #BML-ST415-0001), 200 µM CK666 (Sigma ...
• Sequential combinations of chemotherapeutic agents with BH3 mimetics to treat rhabdomyosarcoma and avoid resistance

  Clara Alcon, et al., bioRxiv - Cancer Biology 2020

  Quote: ... alamethecin (Enzo Life Sciences, BML-A150-0005), the different BIM BH3 concentrations used ...
• The UPR^mt Preserves Mitochondrial Import to Extend Lifespan

  Nan Xin, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-HSP-6 antibody (Enzo Life Science ADI-SPS-825); and anti-NDUF3 [17D95] antibody (Abcam ab14711) ...
• HDAC4 controls senescence and aging by safeguarding the epigenetic identity and ensuring the genomic integrity

  Eros Di Giorgio, et al., bioRxiv - Cell Biology 2020

  Quote: ... 20µM Etoposide (Enzo Life Sciences), 100nM ABT-263 (Clinisciences).
• HDAC4 controls senescence and aging by safeguarding the epigenetic identity and ensuring the genomic integrity

  Eros Di Giorgio, et al., bioRxiv - Cell Biology 2020

  Quote: ... 3.125µm Camptothecin (Enzo Life Sciences), 20µM Etoposide (Enzo Life Sciences) ...
• Near-atomic structures of the BBSome reveal a novel mechanism for transition zone crossing

  Kriti Bahl, et al., bioRxiv - Cell Biology 2020

  Quote: Pathway agonists were used in the following concentration: SAG (200 nM; ALX-270-426-M001; Enzo Life Sciences), somatostatin-14 (sst ...
• RhoA effectors LOK/SLK activate ERM proteins to locally inhibit RhoA and define apical morphology

  Riasat Zaman, et al., bioRxiv - Cell Biology 2020

  Quote: ... Calyculin A (Cat #BML-El92-0100) was purchased from Enzo Life Sciences and Blebbistatin (Cat #B592500 ...
• Microtubules tune mechanosensitive cell responses

  Shailaja Seetharaman, et al., bioRxiv - Cell Biology 2020

  Quote: ... RGD peptide (Enzo Life Sciences) and RGE Control peptide (Enzo Life Sciences ...
• Microtubules tune mechanosensitive cell responses

  Shailaja Seetharaman, et al., bioRxiv - Cell Biology 2020

  Quote: ... and RGE Control peptide (Enzo Life Sciences) were added prior to wounding ...
• TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALS

  Kathleen M. Cunningham, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-poly ubiquitin FK1 (Enzo Life Sciences) 1:200 ...
• TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALS

  Kathleen M. Cunningham, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-poly ubiquitin FK1 (Enzo Life Sciences #BML-PW8805) 1:1000 ...
• TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALS

  Kathleen M. Cunningham, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-poly ubiquitin FK1 (Enzo Life Sciences #BML-PW8805) 1:200 ...
• Tryptophan residues in TDP-43 and SOD1 mediate the cross-seeding and toxicity of SOD1

  Edward Pokrishevsky, et al., bioRxiv - Cell Biology 2020

  Quote: ... and incubated in primary antibodies overnight: 1:500 rabbit anti-SOD1-100 (Enzo Life Sciences, ADI-SOD-100) and 1:100 mouse anti-HA (Abcam ...
• Tryptophan residues in TDP-43 and SOD1 mediate the cross-seeding and toxicity of SOD1

  Edward Pokrishevsky, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-SOD1-100 antibody (#ADI-SOD-100, Enzo Life Sciences), mouse anti-HA antibody (#ab130275 ...
• Zn²⁺ influx activates ERK and Akt signaling pathways through a common mechanism

  Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer, bioRxiv - Cell Biology 2020

  Quote: ... we performed an in-vitro plate-based fluorescence assay using recombinant human MKP-3 (Enzo Life Sciences) supplied in 20 mM Tris/HCl ...
• ERRα coordinates actin and focal adhesion dynamics

  Violaine Tribollet, et al., bioRxiv - Cell Biology 2020

  Quote: ... Hsp90 (ADI-SPA-830, Enzo life science), MAP4K4 (3485 ...
• Autophagy Compensates for Lkb1 Loss to Maintain Adult Mice Homeostasis and Survival

  Khoosheh Khayati, et al., bioRxiv - Cell Biology 2020

  Quote: ... p62 (Enzo Life Sciences, PW9860-0100), LC3 (Nano Tools ...
• MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1α

  Vera Kovaleva, et al., bioRxiv - Cell Biology 2020

  Quote: ... For nuclear and endoplasmic reticulum staining we used ER-ID® Red assay kit (ENZ-51026-K500, Enzo Life Sciences), containing Hoechst 33342 nuclear stain and ER-ID® Red detection reagent ...
• MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1α

  Vera Kovaleva, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-PDI (ADI-SPA-891-F, Enzo Life Sciences), mouse anti-GM130 (610823 ...
• Differentiation drives widespread rewiring of the neural stem cell chaperone network

  Willianne I. M. Vonk, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Proteostat and Hoechst stainings were done according to manual instructions (Enzo Life Sciences), and fluorescence was measured in a Tecan fluorimeter (Proteostat ...
• The COVID-19 immune landscape is dynamically and reversibly correlated with disease severity

  Hamid Bolouri, et al., bioRxiv - Immunology 2020

  Quote: ... Blood cells were washed twice with phosphate-buffered saline (PBS) and stained for viability exclusion with a 100 µM cisplatin solution (Enzo Life Sciences, Farmingdale, New York) for one minute at room temperature ...
• A cysteine protease inhibitor blocks SARS-CoV-2 infection of human and monkey cells

  Drake M. Mellott, et al., bioRxiv - Biochemistry 2020

  Quote: ... and Z-LR-AMC (Enzo Life Sciences, Inc.). Recombinant SARS-CoV-1 spike protein was obtained from SinoBiological and SARS-CoV-2 spike was obtained from Genscript and Acro Biosystems.
• Spinal cord precursors utilize neural crest cell mechanisms to generate hybrid peripheral myelinating glia

  Laura Fontenas, Sarah Kucenas, bioRxiv - Developmental Biology 2020

  Quote: ... dechorionated foxd3:mcherry;nkx2.2a:megfp embryos were treated with 100 μM GM6001 (Enzo Life Sciences)/1% DMSO in PTU egg water from 36 to 72 hpf ...
• Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells

  Diana Gumber, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 5 μM (Enzo Life Science). The H9 SOX17:GFP cell line was kindly provided by material transfer agreement by Dr ...
• The C. elegans proteasome subunit RPN-12 is required for hermaphrodite germline sex determination and oocyte quality

  Lourds M. Fernando, et al., bioRxiv - Developmental Biology 2020

  Quote: ... a mouse monoclonal proteasome 20S core particle antibody that recognizes alpha 1,2,3,4,5,6,7 subunits (1:200, Enzo Life Sciences, Cat. No. BML-PW8195) and rabbit GFP antibody (1:500 ...
• Elucidation of the signatures of proteasome-catalysed peptide splicing

  Wayne Paes, et al., bioRxiv - Immunology 2020

  Quote: ... 5μg of synthetic polypeptide were incubated with 500ng of the 20S constitutive proteasome (CP) (Enzo Life Sciences) in 20mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
• Distinct Cdk9-phosphatase switches act at the beginning and end of elongation by RNA polymerase II

  Pabitra K. Parua, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Colorimetric assays were performed in triplicate using BioMOL Green (Enzo Life Sciences) in 25 mM HEPES (pH 7.5) ...
• Transgenic goats producing an improved version of cetuximab in milk

  Götz Laible, et al., bioRxiv - Molecular Biology 2020

  Quote: ... The detecting antibody used was a mouse IgM to alpha 1,3 gal (ALX-801- 090, Enzo Life Sciences, Farmingdale, NY, USA). The secondary antibody was HRP goat anti-mouse (SC2064 ...
• Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme

  Roman O. Fedoryshchak, et al., bioRxiv - Molecular Biology 2020

  Quote: ... reactions were quenched by addition of 100 µl of Biomol Green reagent (Enzo Life Sciences) for 30 min ...
• FUS-ALS mutants alter FMRP phase separation equilibrium and impair protein translation

  N. Birsa, et al., bioRxiv - Neuroscience 2020

  Quote: ... and plated in Nutristem-XF/FF medium (Biological Industries) supplemented with 10 μM rock inhibitor (Enzo Life Sciences) on Matrigel (BD Biosciences ...
• Single Cell Analysis Reveals Multi-faceted miR-375 Regulation of the Intestinal Crypt

  Michael T. Shanahan, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and 10 mM Y27632 (Enzo Life Sciences, Farmingdale, NY, cat. ALX270-333-M025) was added ...
• Plasmodium falciparum guanylyl cyclase-alpha and the activity of its appended P4-ATPase domain are essential for cGMP synthesis and blood stage egress

  Stephanie D. Nofal, et al., bioRxiv - Molecular Biology 2020

  Quote: Intracellular cyclic nucleotide levels in mature segmented schizonts were measured using ELISA-based high-sensitivity direct cAMP and cGMP colorimetric assay kits (Enzo life sciences). Around 1.25×108 schizonts were Percoll-purified were obtained from RAP- and DMSO-treated cultures to which C2 (1.5 μM ...
• PH domain and leucine rich repeat phosphatase 1 (Phlpp1) suppresses parathyroid hormone receptor 1 (Pth1r) expression and signaling during bone growth

  Samantha R. Weaver, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Urine creatinine and cAMP levels were measured via ELISA (Enzo Life Sciences, Farmingdale ...
• Increased processing of SINE B2 non coding RNAs unveils a novel type of transcriptome de-regulation underlying amyloid beta neuro-pathology

  Yubo Cheng, et al., bioRxiv - Genomics 2020

  Quote: ... His-tagged Hsf1 (Enzo Life Sciences: ADI-SPP-902-F). Hsf1 working concentrations were 250nM unless otherwise specified ...
• Instability of aquaglyceroporin (AQP) 2 contributes to drug resistance in Trypanosoma brucei

  Juan F. Quintana, et al., bioRxiv - Microbiology 2020

  Quote: Ubiquitylated proteins were isolated using the UbiQapture-Q kit (Enzo Life Sciences, Farmingdale, New York, USA) according to the manufacturer’s instructions ...
• Differential TAM receptor regulation of hepatic physiology and injury

  Anna Zagórska, et al., bioRxiv - Physiology 2020

  Quote: ... and LPS (ENZO life sciences, Cat# ALX-581-013-L002 ...
• Altered hypothalamic DNA methylation and stress-induced hyperactivity in a novel model of early life stress

  Eamon Fitzgerald, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Plasma corticosterone was analysed by ELISA (Enzo Life Sciences, Exeter).
• Inhibiting Mycobacterium tuberculosis CoaBC by targeting a new allosteric site

  Vitor Mendes, et al., bioRxiv - Biochemistry 2020

  Quote: ... Phosphate was then detected using the BIOMOL® Green reagent (Enzo Life Sciences), which when bound to phosphate absorbs light at 650 nm ...
• KAT2-mediated acetylation switches the mode of PALB2 chromatin association to safeguard genome integrity

  Marjorie Fournier, et al., bioRxiv - Cell Biology 2020

  Quote: ... or 2.5 μM olaparib (Enzo Life Sciences LKT-O4402). DMSO was used as negative vehicle control ...
• Reconstitution of human kinetochore in mitotic cell extracts reveals permitted and restricted assembly steps

  Ekaterina V. Tarasovetc, et al., bioRxiv - Cell Biology 2020

  Quote: ... phosphatase inhibitors (100 ng ml−1 microcystin-LR (Enzo Life Sciences), 1 mM sodium pyrophosphate (Sigma-Aldrich) ...
• Identifying therapeutic drug targets for rare and common forms of short stature

  Karol Estrada, et al., bioRxiv - Genetics 2020

  Quote: ... cells were then incubated in DMEM containing 0.75 mM IBMX (3-isobutyl-1-methylxanthine) (Enzo life sciences) for 15 min ...
• FTO intronic SNP strongly influences human neck adipocyte browning determined by tissue and PPARγ specific regulation: a transcriptome analysis

  Beáta B. Tóth, et al., bioRxiv - Cell Biology 2020

  Quote: ... and oligomycin (Enzo Life Sciences cat#ALX-380-037) inhibited OC and ECAR were recorded ...
• Toll-like receptor 4 is activated by platinum and contributes to cisplatin-induced ototoxicity

  Ghazal Babolmorad, et al., bioRxiv - Pharmacology and Toxicology 2020

  Quote: ... we used the Total ROS-ID detection kit (Enzo Life Sciences, ENZ-51011). Cells were trypsinized and washed with 1X ROS Wash Buffer ...
• TANGO1 membrane helices create a lipid diffusion barrier at curved membranes

  Ishier Raote, et al., bioRxiv - Cell Biology 2020

  Quote: ... calreticulin (goat anti–human; Enzo Life Sciences), HA (mouse ...