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Citations for Bio-Rad :
101 - 125 of 125 citations for Tissue Grinders since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Cancer Biology 2022Quote: ... The fixed and stained gene expression slide was treated with a permeabilization enzyme specific for mouse tongue determined by the Spatial tissue optimization slide kit (10x Genomics, #1000191) for 24 minutes on the thermal cycler (Bio-Rad, 1851197). Synthesis of cDNA and second strand were performed using reverse transcriptase and second strand reaction (primer and enzyme) ...
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bioRxiv - Neuroscience 2020Quote: ... brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Immunology 2021Quote: ... The mRNA expression level of the brain tissue was detected by qRT-PCR via iQ SYBER green I Supermix (Bio-Rad, USA) and a Bio-Rad RT-PCR instrument system ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Immunology 2023Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from the cerebellum using the Aurum™ Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hercules, CA, USA). Reverse transcription was performed using the iScript™ cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: Mouse patellofemoral joints and hearts were homogenized using an electric homogenizer with toothed blades in tissue lysis buffer (Machery-Nagel, 740962) containing beta-mercaptoethanol (1:100) (Bio-Rad, 161-0710) and Triton-X-100 (1:100 ...
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bioRxiv - Neuroscience 2019Quote: ... Instructions were followed as per the Bio-Rad Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hemel Hempsted, UK, Cat# 7326830) and our previously optimised protocol (Pervolaraki et al. ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from the pool of 25 embryos (or dissected embryo tissue) using PureZOL RNA Isolation Reagent (Bio-Rad, #732-6890) and Aurum Total RNA Isolation Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... The total amount of protein isolated from the weighed tissues was determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). Hydroxyproline content data are expressed as μg of collagen per mg of total lung protein (μg/mg).
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of purified RNAs from the control and treated tissues were reverse-transcribed and amplified using Brilliant SYBR Green QRT-PCR Master Mix (Bio-Rad, Hercules, CA). Human β-actin was used as an internal control ...
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bioRxiv - Immunology 2023Quote: ... Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue) were reverse transcribed to cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891) and stored at −20°C ...
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bioRxiv - Zoology 2024Quote: ... or tissue-specific total RNA (80ng) was used as a template to synthesize cDNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, Mississauga, ON) followed by an 11-fold dilution with nuclease-free water prior to RT-qPCR analysis.
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bioRxiv - Microbiology 2024Quote: ... One hundred nanograms of gDNA from each tissue type or isolate were used to perform ddPCR assay using a QX200 Droplet Digital PCR SystemTM (Bio-Rad, Hercules, USA), as described by von Ammon et al ...
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bioRxiv - Biochemistry 2019Quote: ... was performed to analyze the expression pattern of selected P450 candidates in different tissues (fat body, gut, Malpighian tubules and glands) with Bio-Rad CFX96 real time PCR detection system (Bio-Rad Laboratories, Munich, Germany). Each RT-qPCR reaction (20 μl final volume ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
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bioRxiv - Physiology 2023Quote: ... TNF-α) concentrations in blood plasma samples and tissue homogenate supernatants were determined with Bio-Plex Pro magnetic multiplex assays (Bio-Rad; Hercules, CA) and analyzed on the Bio-Plex 200 system with Bio-Plex Manager 5.0 software (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: cDNA was synthesized from total RNA (200 ng/µl) extracted from brain tissue using the reverse transcription supermix iScript TM for RT-qPCR (Bio-Rad, Carolina, United States) according to the manufacturer’s indications ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of whole-cells and tissue extract (20-60 µg) were separated on 15% SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Mississauga, ON, Canada). The membranes were first blocked with 5% milk [w/v] in TBS/0.05% Tween 20 [v/v] for 60 min at room temperature and subsequently blotted overnight at 4º C with primary antibodies (see Table 2) ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Systems Biology 2021Quote: ... plates were centrifuged for 10 min at 2000g and heated at 95C for 30 min (cell culture) or 60 min (tissue) in a thermal cycler (Biorad S1000 with 384-well reaction module) at a constant lid temperature of 110 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins (20-30 μg) of the tissue protein samples were subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad mini-PROTEAN TGX Gels, # 4561044) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2020Quote: ... This sequence was amplified from B6J genomic DNA extracted from spleen tissue using iProof High-Fidelity PCR kit (BIO-RAD, Hercules, CA, USA; Cat# 172-5330) with the following primers containing XhoI and HindIII restriction enzyme sites in the sense and antisense primers respectively ...