Labshake search
Citations for Bio-Rad :
51 - 100 of 125 citations for Tissue Grinders since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... 2×106 cells (extracted tissues or cell culture) were suspended in 50 μL RIPA (Radio ImmunoPrecipitaion Assay; BioRad) lysis buffer containing 200 mM PMSF (Alpha-Phenyl Methyl Sulfonyl Fluoride ...
-
bioRxiv - Cancer Biology 2020Quote: ... The tissue array was then washed twice with PBS and secondary rabbit anti-FITC antibody (Biorad, #4510-7804) was added to a final 1:1000 dilution in 2% BSA/PBS at room temperature for 1h ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was purified from homogenized SKM and BAT tissues using PureZOL™ RNA Isolation reagent (BioRad, CA) as described [23,24] ...
-
bioRxiv - Physiology 2023Quote: ... Protein concentrations in supernatant tissue homogenate samples were determined with the Bradford method (Bio-Rad Laboratories; Richmond, CA) and measured on a Spectramax 190 Plate Reader (Molecular Devices LLC ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
-
bioRxiv - Physiology 2023Quote: ... cell or tissue lysates were resolved by SDS-PAGE and transferred to nitrocellulose membrane (Bio-Rad Laboratories, USA). Blots were blocked with 5% nonfat milk in phosphate-buffered saline (PBS) ...
-
bioRxiv - Immunology 2023Quote: RNA was isolated from xenoengrafted TA muscles using Aurum Total RNA Fatty and Fibrous Tissue kit (Bio-Rad) per the manufacturer’s specifications ...
-
bioRxiv - Neuroscience 2024Quote: ... Total protein (20-40 μg) from tissue supernatant was loaded into TGX precast gels (4–20% CriterionTM, BioRad) and transferred to nitrocellulose membrane (AmershamTM ProtranTM ...
-
bioRxiv - Immunology 2024Quote: ... Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad). RNA from epithelial cells and tissue was isolated by following the TRIzol extraction manufacture protocol ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Genomic DNA was isolated from tail clippings or liver tissue using the Chelex Instagene-matrix extraction protocol (Bio-Rad).
-
bioRxiv - Molecular Biology 2019Quote: ... Total RNA was then extracted using the Aurum Total RNA Mini Kit for Fatty and Fibrous Tissue (Bio-Rad). Reverse transcription reactions were performed using the iScript cDNA Synthesis Kit (Bio-Rad ...
-
bioRxiv - Cancer Biology 2022Quote: cDNA synthesized from cells and animal tumors tissues was used for real-time PCR performed on iCycler (Bio-Rad) using a 2x SYBR Green master mixture (Bio-Rad ...
-
bioRxiv - Neuroscience 2020Quote: EV samples and brain tissue homogenate samples were run in a 4% to 20% gradient gel (# 4561093 Bio-Rad) and electro-transferred to Immobilon-P membrane ...
-
bioRxiv - Immunology 2019Quote: ... and phenolchloroform extraction from whole-lung tissues of mice and quantified on a SmartSpecTM Plus spectrophotometer (Bio-rad, USA) (Liu et al ...
-
bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged to pellet tissue debris and equal volumes mixed 1:1 with 2x Laemmli sample buffer (BioRad). Samples were boiled ...
-
bioRxiv - Immunology 2020Quote: cDNA was synthesized from the indicated tissue RNA using iScript cDNA synthesis kit (#170-8891) acquired from Bio-Rad according to the manufacturer’s recommendations ...
-
bioRxiv - Plant Biology 2023Quote: ... The tissues were bombarded on the same day as isolation using 0.6μm gold particles (Bio-Rad Laboratories Ltd, UK) coated with the constructs pRRes208.617 ...
-
bioRxiv - Neuroscience 2023Quote: ... 25 µl cell lysate and 50 µg protein for tissue lysates was loaded on 4-15% (Bio-Rad, 5678084) and 10% TGX Stain-Free Protein Gels (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: Cortical and hippocampal brain tissue sections were lysed using the Bio-Plex cell lysis kit (Bio-Rad Laboratories #171304011) and protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher #23225) ...
-
bioRxiv - Microbiology 2023Quote: ... The burden in blood and tissues was determined by qPCR with the Sso Fast EvaGreen Supermix assay (Bio-Rad) using the flaB2 gene ...
-
bioRxiv - Bioengineering 2022Quote: Total RNA was extracted and purified from RPE tissue punches using Single Shot Cell Lysis Kit (Bio-Rad Laboratories). cDNA synthesis was performed from 1.5 μg RNA using RT² First Strand Kits (Qiagen ...
-
bioRxiv - Systems Biology 2023Quote: ... The heart tissue samples were then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 20% v/v sodium dodecyl sulfate (SDS ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 μg total liver tissue homogenate was run 1.5 cm into a 12.5% SDS–PAGE gel (Criterion, Bio-Rad). This was followed by fixation and staining with GelCode Blue (Pierce) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Following washes in PBS tissues were counterstained with DAPI (VECTASHIELD, LSBio) and mounted in gaskets (BioRad Seal Frame Incubation Chambers) on glass cover slips ...
-
bioRxiv - Cell Biology 2020Quote: ... In short: OCT is removed with PBS and then tissue is placed in a cold 4% polyacrylamide (1610140 Bio-Rad) hydrogel with 0.25% photoinitiator (VA-044 ...
-
bioRxiv - Immunology 2019Quote: ... Donor CD103+ KLRG1-CD8 T cells from LP of transplanted tissue were identified following the gating strategy in FigureS11 and single cells were sorted into 96-well plates (BioRad) containing 5 µL capture buffer (20 mM Tris-HCl pH8 ...
-
bioRxiv - Cancer Biology 2019Quote: RNA was isolated from cell lines or flash-frozen tissue as described above was reverse-transcribed using iScript (Bio-Rad). Real-Time PCR was conducted using VeriQuest SYBR Green qPCR Master Mix (Affymetrix) ...
-
bioRxiv - Neuroscience 2019Quote: The amount of protein was prepared from homogenized tissues or tells and determined using the Bradford quantification assay (Bio-Rad). Protein extracts were separated by SDS-PAGE and electroblotted to PVDF membrane (Millipore ...
-
bioRxiv - Cancer Biology 2021Quote: Small pieces of dissected frozen pancreatic tumor tissues were collected and lysed (Bio-Plex Cell Lysis Kit, BioRad, USA; 171304011), followed by a repeated procedure of vortexing ...
-
bioRxiv - Immunology 2021Quote: ... The amount of protein in each tissue sample was quantified using detergent compatible protein assay (Bio-Rad, Hercules, CA, USA). Then ...
-
bioRxiv - Plant Biology 2019Quote: Total RNA from seedling or leaf tissue of the primary transformants (T1) was isolated using the PureZOL reagent (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2021Quote: Tissue lysates (30 μg protein) were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against ROCK1 (Cat# ...
-
bioRxiv - Genetics 2022Quote: Total RNA was extracted from kidney and cells using Aurum TM Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad) or RNAqueous™ Micro Total RNA Isolation Kit (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: 10 μL of tissue samples (25 μg of protein per well) were placed into each well of the Western blot gel (Biorad Criterion TGX Stain free 4-15% 18 wells ...
-
bioRxiv - Neuroscience 2022Quote: ... Approximately 20 μg of total proteins in tissue lysates were separated on electrophoresis using 4 - 12% gradient SDS-polyacrylamide gels (BioRad) and transferred to the PVDF membrane (BioRad) ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein samples from cultured cells or mouse tissue were prepared by diluting 20-40 μg of protein with 1X Laemmli buffer (Biorad) supplemented with beta-mercaptoethanol (Sigma ...
-
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The viral genome concentration (including in mouse tissue) was determined by ddPCR in a QX200 Droplet Digital PCR System (BioRad), using Taqman primers/probe against the CMV enhancer (Sup ...
-
bioRxiv - Physiology 2023Quote: ... The spleen was removed and processed in tissue protein extraction reagent for cytokine analysis and protein quantification using a standard Bradford protein assay kit (Biorad). Subsequently tissues and serum were analyzed for cytokine levels using standard ELISA kits ...
-
bioRxiv - Plant Biology 2023Quote: ... leaf tissue was ground in liquid N2 and total RNA was extracted using the Aurum Total RNA Mini Kit (BioRad) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA samples from monotreme tissues were reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). RNA was stored in nuclease free water at -80°C.
-
bioRxiv - Immunology 2024Quote: ... Tissue lysates were separated by centrifugation and separated by SDS-PAGE electrophoresis and transferred onto a nitrocellulose membrane (Bio-Rad). The membrane was blocked for 1 hr in 5% skimmed milk followed by incubation with primary antibody specific for SARS-CoV- 2 nucleocapsid protein (1:300 ...
-
bioRxiv - Neuroscience 2022Quote: RNA isolation for all tissues were carried out using the Aurum Total RNA isolation kit (BioRad #732-6820; Hercules, CA, USA). Samples were reverse-transcribed to cDNA using qScript cDNA Supermix (Quantabio #101414-106 ...
-
bioRxiv - Genetics 2022Quote: ... or 25 µg (mouse tissues) of proteins were loaded per lane on Any kD or 4-20% Criterion TGX stain-free precast gels (Bio-Rad) and transferred to Immobilon-FL PVDF membrane (Millipore ...
-
bioRxiv - Developmental Biology 2023Quote: ... 0.1 µg RNA (sorted cells) or 1 µg RNA (tissues) was used to prepare cDNA with the iScript™ cDNA synthesis kit (Biorad) with the following protocol ...
-
bioRxiv - Developmental Biology 2023Quote: Whole cell and tissue protein was extracted using the Active Motif protein extraction Kit and quantified with the Bradford assay (Bio-Rad). Snap-frozen cardiac apical tissues were homogenized using Dounce tissue grinder (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2023Quote: Immunoassays were performed with 25 μl of conditioned medium or tissue lysate each reaction using a Bio-Plex 200 System (Bio-Rad) with Milliplex Map Mouse Cytokine/Chemokine Panel I (Millipore ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from PMVECs and Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
-
bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
-
bioRxiv - Neuroscience 2021Quote: Western blotting experiments were performed on hippocampal tissue and specific bands were visualized with secondary HRP-conjugated antibodies using ChemiDoc™ Imaging Systems (Bio-Rad). The relative intensities of immunoblot bands were determined by densitometry with ImageJ software ...
-
bioRxiv - Zoology 2019Quote: An equal amount of protein from each one of three liver or spleen samples (each tissue was harvested from a different bird) was loaded on a Criterion XT 4-12 % gradient polyacrylamide gel (Bio-Rad, USA) and proteins were separated by SDS-PAGE ...