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Citations for Bio-Rad :
1 - 50 of 10000+ citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Biochemistry 2021Quote: ... pH 5.3 (murine P-domains) or pH 7.3 (GII.4 Saga P-domain) at 4 °C via Micro Bio-Spin 6 columns (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing protein (determined by Bradford assay; Biorad) were concentrated using a 10,000 MWCO centrifugal filter (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants containing protein fractions were quantified using the Protein Assay kit (Bio-Rad), with bovine serum albumin used to generate a reference quantification curve.
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed and blocked in 1x TBST with 5% bovine serum albumin before consecutive incubation with primary antibody (see Suppl. Table 3) and horseradish peroxidase-coupled protein A (Cymed) and detection using Clarity Western ECL Substrate (BioRad).
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bioRxiv - Molecular Biology 2021Quote: Supernatants containing protein contents were determined by Bradford Protein Assay (Bio-Rad Laboratories, Hercules, CA). Proteins were immunoblotted with anti-PPARα (ab24509 ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration of VSW-3 RNAP was determined by Bradford protein quantitative kit (Bio-rad), and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing protein were collected and the protein concentration was measured using Bradford dye (BCA, Biorad). The lysate concentration was normalized and Bolt LDS Sample Buffer (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE containing proteins were transferred onto PVDF membranes (Bio-Rad) using a semidry transfer cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein-containing membranes were incubated in 5% blocking reagent (Bio-Rad) dissolved in 1X TBST (99% Tris buffered saline and 1% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... The protein lysates containing 20 micrograms of protein were separated in 4-15% polyacrylamide gel (Bio-Rad) with 125 V for 100 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated supernatant containing total cellular protein was then quantified using a DC Protein Assay (Bio-Rad Laboratories) performed as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... and protein-containing fractions and purity was determined by SDS-PAGE (BioRad). The following day ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of proteins were mixed !3-mercaptoethanol-supplemented Laemmli buffer (BioRad), heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... All Blue Prestained Protein Standards (1-3 µl; BioRad Laboratories, Hercules, CA) were used to identify band molecular weights ...
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bioRxiv - Bioengineering 2022Quote: ... along with 3 μL of Precision Plus protein ladder (Bio-Rad #1610374), and gels were run at 125 V for 1.25 hours in Tris-Glycine running buffer (ThermoFisher #LC2675).
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bioRxiv - Biochemistry 2024Quote: ... proteins were separated by Criterion XT Tris Acetate 3-8% (Bio-rad) SDS-PAGE and visualized by silver staining.
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bioRxiv - Cell Biology 2021Quote: ... proteins were separated on a 3-8% Criterion XT tris-acetate gel (Biorad) or 4-15% Criterion TGX gel according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... with 3 uL Precision Plus Protein All Blue prestained standards (Bio-Rad, #1610373) loaded as a ladder ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants containing the protein extracts were transferred into fresh 1.5 ml tubes and protein concentration was measured by Bio-Rad Protein Assay Kit using BSA as protein standard (5000002 ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein-antibody complexes were collected by incubating protein extracts with 20 μl SureBeads Protein G magnetic beads (Bio-Rad) for 30 min at room temperature on a rocking shaker ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Mouse anti Tubulin beta 3 antibody from BioRad (Hercules, CA, US). We used a 6xHis-GFP-labeled truncated human kinesin-1 heavy chain construct (amino acids 1-560 ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... The band containing all proteins was visualized with Biosafe Coomassie G-250 stain (Biorad) and excised from gel ...
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bioRxiv - Physiology 2022Quote: ... The supernatant was mixed with SDS protein sample buffer containing β-mercaptoethanol (Bio-Rad) and resolved by SDS-PAGE on 4-20% Mini-Protean GTX Stain-Free gels (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins in the samples were denatured using sample buffer containing SDS (BioRad, 1610747) and subsequently transferred to a PVDF membrane (Millipore Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... kinase-dead extracts were incubated 3 consecutive times with protein A-beads (Bio-Rad) previously coated with the anti-B55δ antibody (1 hour incubation at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 μg of the protein samples were prepared with 4X Laemmli loading buffer (Biorad) and denatured at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-bound DNA was recovered using the Protein A or Protein G magnetic beads (Bio-Rad), washed similarly as DRIP samples and treated with Proteinase K and RNAse after elution ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples containing 236 µg protein lysate in final 96 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006) were then prepared for subsequent reduction and alkylation for 25 min at room temperature by the simultaneous addition of 10X tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... including unmodified antibodies and a protein ladder (Bio-rad, #1610375) were also used ...
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bioRxiv - Biochemistry 2022Quote: ... Total lysate containing the equivalent of 1.1 mg of protein (Easy Bradford BioRad, BSA standards) was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were diluted (3:1) with a 4x Laemmli sample buffer (Biorad, Cat# 1610747) and heated at 98 °C for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by 3-8% or 8-16% SDS-PAGE (NU-PAGE or BioRad) and transferred to immobilon-P membranes (Millipore) ...
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bioRxiv - Genomics 2023Quote: ... 3-2) Protein Enrichment: The serum samples were processed with a ProteoMiner kit (Bio-Rad) and the protein concentration was determined using the BCA and fluorescence assays ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 3 µg protein was separated by SDS-PAGE gel electrophoresis (Bio-Rad) and transferred to 0.2 µm nitrocellulose membrane (Amersham™ Protran™ ...
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bioRxiv - Microbiology 2023Quote: ... 40 µg of proteins were loaded onto 3–8% precast polyacrylamide gel (Bio-Rad, 3450129). After migration ...
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bioRxiv - Cell Biology 2024Quote: ... The mitochondria-containing pellet was resuspended in THE buffer without BSA and the protein concentration measured using the Bradford Protein Assay (Bio-Rad, Munich, Germany). The protein concentration was adjusted to 10 µg/µl with THE buffer.
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bioRxiv - Physiology 2024Quote: ... Primary antibodies were diluted in TBS-T containing 5% dry nonfat milk (Biorad). Membranes were incubated overnight at 4 °C with primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked in PBS containing 5% goat serum (Bio-Rad Antibodies) and 0.1% saponin (SigmaAldrich ...
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bioRxiv - Microbiology 2020Quote: The spots containing the proteins of interest were cut using the EXQuest Spot Cutter (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... The protein sample was diluted with Laemmli buffer containing β-mercaptoethanol (Bio-rad, Hercules, CA, USA) and incubated at 65°C for 5 min ...