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Citations for Bio-Rad :
551 - 600 of 697 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then transferred onto low-fluorescence 0.45 μm PVDF membranes with transfer buffer (10× Tris/glycine with 10% methanol) (Bio-Rad) for 10 min using the semi-dry Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast concentration was adjusted to 4 × 108 cells/ml and added to the same amount of 1.2% low melting agarose gel (Bio-Rad) solution ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast suspensions (2 × 108 cells/ml) containing 0.6% low melting agarose gel were added to 50-well dispensable mold plates (Bio-Rad). Plugs were immersed in 10 ml of NDS (1% N-lauroyl sarcosinate sodium salt solution ...
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bioRxiv - Bioengineering 2020Quote: ... Lysis plates were prepared by dispensing 0.4 µL of lysis buffer into each well of a 384 well hard-shell low profile PCR plate (Bio-rad) using liquid handler Mantis (Formulatrix) ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were then PCR amplified: 50 μl reactions (25 μl SsoFast Evagreen supermix with low ROX (Bio-Rad, Hercules, CA), 0.6 μl 25 μM custom Nextera PCR primer 1 ...
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bioRxiv - Cell Biology 2021Quote: ... was gently mixed with 25 μl of molten 1.5 % low-melting-point agarose and pipetted into a casting mold (Bio-rad), which was placed on ice for 5 min to let agarose plugs solidify ...
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bioRxiv - Cell Biology 2021Quote: ... was gently mixed with an equal volume of molten 1.5 % low-melting-point agarose and pipetted into a casting mold (Bio-rad), which was placed on ice for 10 min to solidify the agarose ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation and spotted on a small block of TAP + 1.5% low-melting-point agarose (Bio-Rad), which was then placed in a glass-bottomed 18-well chamber (Ibidi ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants were transferred to pre-chilled low-adhesion 1.5 mL tubes and quantified by Bradford (cat. no. 5000006, Bio-Rad). Prior to loading ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants were transferred to pre-chilled low-adhesion 1.5 mL tubes and quantified by Bradford (cat. no. 5000006, Bio-Rad). After quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... and transferred to low fluorescence PVDF membranes with the Trans-Blot Turbo Transfer System according to manufacturer’s instructions (Bio-Rad). Membranes were blocked for one hour with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a 0.2 µm or a low-fluorescence 0.45 µm polyvinylidene difluoride (PVDF) transfer membrane using a Trans-Blot Turbo (Bio-Rad). Following antibody incubation ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... 2.25 ul of pre-amplified cDNA was mixed with 2.5 ul of 2X SsoFast EvaGreen Supermix with low ROX (Bio-Rad) and 0.25 ul of 20X DNA binding dye sample loading reagent (Fluidigm ...
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bioRxiv - Plant Biology 2023Quote: Shoot apices of reporter lines were dissected and fixed in 4% paraformaldehyde for 1 hour and embedded in 7% ultra low range agarose gel (BioRad). Fifty µm sections were prepared using a vibratome DTK-1000 (Dosaka ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 μl of this cell suspension was then placed on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) TGY2X pad prepared on a cover glass using a gene frame (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... qRT-PCR reactions were assembled using SsoFast™ EvaGreen® Supermix with Low ROX (Bio-Rad Laboratories, Inc., Hercules, CA) following manufacturer’s protocol ...
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bioRxiv - Pathology 2024Quote: ... The qPCR was performed with Fluidigm’s 48.48 dynamic array IFC on the Biomark HD using 2X SsoFast EvaGreen Supermix with Low Rox (Bio-Rad Laboratories), 20X DNA Binding Dye Sample Loading Reagent (Fluidigm) ...
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bioRxiv - Microbiology 2023Quote: ... The protein was eluted the next day and was buffer exchanged into a low salt buffer (75mM HEPES, pH 7.6, 100mM KCl, 10% glycerol, 2mM DTT) using a 10-DG column (Bio-rad) for further purification ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed for 1 hour at room temperature with 1:500 anti-XLF(New England Peptides, details in Methods under Immunodepletion) or 1:1000 anti-His (Bio-Rad MCA1396A) in PBST with 2.5% BSA ...
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bioRxiv - Immunology 2019Quote: ... After quantifying the optimal virus-to-erythrocyte concentration (4HA units), serial two-fold dilutions of Fc, control IVIG (GammaGard, Baxter Healthcare) and polyclonal goat anti-influenza B (Biorad) were prepared ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... and a primary rat anti-mouse CD68 antibody (activated microglia/macrophages, 1:200 dilution, MCA1957, Biorad) with secondary goat anti-rat Alexa-Fluor 555 (1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: anti–α-tubulin (MCA77G YL1/2, rat monoclonal; Bio-Rad), anti-GAPDH (mouse ...
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bioRxiv - Cell Biology 2019Quote: Sections of mice peritoneum were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), rabbit anti-mouse CD38 antibody (bs-0979R ...
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bioRxiv - Genetics 2019Quote: ... α-tubulin is monoclonal antibody of rat that can recognize alpha subunit (AbD Serotec/BioRad, MCA77G). The second antibodies for staining were Alexa-fluor 488 (Goat ...
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bioRxiv - Genetics 2021Quote: ... rat anti CD68 (1:200; clone FA-11/MCA1957, Bio-Rad Laboratories Inc, Hercules, CA, USA); rabbit anti LAMP1 (1:200 ...
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bioRxiv - Genetics 2021Quote: ... tissue sections were stained with F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2022Quote: ... monoclonal rat anti-F4/80 antibody (dilution 1:200) (clone Cl:A3-1, MCA497G, Biorad Laboratories, Germany) After multiple washes ...
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bioRxiv - Neuroscience 2023Quote: ... RRID:AB_839504) 1:1000 and rat monoclonal CD-68 (Cluster of Differentiation 68; Bio-Rad, MCA1957, RRID:AB_322219) 1:750 overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Gene analysis of TREM2 (PrimePCR™ SYBR® Green Assay: TREM2 for Rat; BioRad, CA, USA) was performed following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... anti-CD31 (rat monoclonal clone ER-MP12, #MCA2388T, RRID: AB_1101899, BioRad Laboratory; 1:200 for IHC), anti-Nestin (chicken polyclonal ...
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bioRxiv - Immunology 2023Quote: ... and stained with rat anti-mouse CD68 primary antibody (clone FA-11; Bio-Rad, Hercules, CA) followed by biotinylated rabbit anti-rat IgG secondary antibody (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
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bioRxiv - Biochemistry 2022Quote: ... acidified to pH3–4 by adding appropriate amount of TFA and subjected to HPLC purification using a C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad, Hercules, CA, USA). The S-sulfonated FAM237A was eluted from the C4 reverse-phase column by an acidic acetonitrile gradient composed of the acidic solvent A (0.1% aqueous TFA ...
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bioRxiv - Biochemistry 2023Quote: ... and the S-sulfonated FAM237B was eluted from a C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad, Hercules, CA, USA) by an acidic acetonitrile gradient ...
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bioRxiv - Microbiology 2024Quote: ... and HopAM1 and C-terminal Flag-tagged IpaH4 were generated by PCR amplification using primers containing Flag sequences and flanking SacII / PacI off pIB/V5-His templates using iProof DNA polymerase (Bio-Rad; Table S4) and cloned into the pDGOpIE2 vector ...
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bioRxiv - Immunology 2022Quote: ... we amplified cDNA fragments and proceeded with qPCR reactions on CFX96 Thermal Cycler Real Time System (Bio-Rad). The reactions were normalized to 3 housekeeping genes (Gapdh ...
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bioRxiv - Immunology 2022Quote: ... we amplified cDNA fragments and proceeded with qPCR reactions on CFX96 Thermal Cycler Real Time System (Bio-Rad). The reactions were normalized to 3 housekeeping genes (Gapdh ...
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bioRxiv - Plant Biology 2022Quote: ... and the genomic fragments were amplified with iProof high-fidelity DNA polymerase (Bio-Rad, USA; Cat. No. #1725301). Primer sequences for the GFP cloning are in Supplemental Table 2.
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bioRxiv - Genetics 2023Quote: ... The copy number of var and control fragments in each well was calculated using QuantaSoft software (Bio-Rad).
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bioRxiv - Physiology 2022Quote: ... Transfer Packs and protein transfer onto membranes was achieved using the low or mixed molecular weight (MW) preprogrammed protocol in a Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA (Millipore Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 9.0 running buffer and visualized using the FITC channel set at 100 µM resolution in low intensity mode on a GelDoc Pharos imager (BioRad Laboratories). ImageQuant software was used to analyse the fraction of probe bound in each lane and GraphPad Prism version 8 software was used to calculate the Kd ...
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bioRxiv - Developmental Biology 2019Quote: ... Single cell qPCR was performed using the Fluidigm BioMark system on 96.96 Dynamic Array IFCs for Gene Expression using Delta Gene Assays and 2X SsoFast EvaGreen Supermix with low ROX (Bio-Rad). For data-processing ...
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bioRxiv - Developmental Biology 2019Quote: ... Single cell qPCR was performed using the Fluidigm BioMark system on 96.96 Dynamic Array IFCs for Gene Expression using Delta Gene Assays and Sso Fast EvaGreen Supermix with Low ROX (Bio-Rad). Single cell qPCR was performed using the Fluidigm BioMark system on 96.96 Dynamic Array IFCs for Gene Expression using Delta Gene Assays and 2X SsoFast EvaGreen Supermix with low ROX (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: Organoids fixed in 4% w/v PFA and immunostained were embedded in 1% w/v low melting agarose (Bio-Rad Laboratories) solution and placed in an Eppendorf tube ...
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bioRxiv - Microbiology 2019Quote: ... 100 μL of culture was removed through the septum using a sterile syringe and transferred to a trUVue low-volume cuvette (Bio-Rad) to read optical density at 600 nm using a SmartSpec Plus spectrophotometer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2019Quote: ... gene expression analysis was achieved on the BioMark HD system (Fluidigm) using SsoFast EvaGreen Supermix with low ROX (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: The purified baculoviruses were electrophoresed on 10% sodium dodecyl sulfate-polyacrylamide gel (BIOTOOLS) and transferred to a low-fluorescence polyvinylidene fluoride (PVDF) membrane (Bio-Rad) through Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Infiltrated leaves were infiltrated with 100 µM D-luciferin and LUC activity was quantified after 48 h using a low-light CCD imaging apparatus (Bio-Rad).