Labshake search
Citations for Bio-Rad :
451 - 500 of 697 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... and rabbit F(ab’)2 anti-rat IgG conjugated to FITC (STAR17B, BioRad).
-
bioRxiv - Neuroscience 2022Quote: ... anti-Ly-6B.2 (1:100, clone 7/4, BioRad, raised in rat) and anti-Nur77 (NR4A1 ...
-
bioRxiv - Neuroscience 2019Quote: ... mouse anti-rat CD2 (1:200; OX-34, Bio-Rad, Hercules, CA, USA). Donkey secondary antibodies conjugated to Alexa Fluor 405/568/647 (Jackson ImmunoResearch ...
-
bioRxiv - Physiology 2020Quote: ... and rat anti-F4/80 (1:500, Clone A3-1, MCA497GA, Biorad, USA). After 3×5 min washes ...
-
bioRxiv - Physiology 2022Quote: ... Liver sections were incubated with rat anti-CD45 (Bio-Rad, MCA1388, 1:500) and rabbit anti-IBA1 for hepatic macrophages ...
-
bioRxiv - Neuroscience 2022Quote: ... Microglia was stained with rat monoclonal anti-CD68 antibody (1:100, Bio-Rad) and with a rabbit monoclonal anti-Iba1 antibody (1:100 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated in primary antibody (rat anti-MBP, BioRad, 1:200 dilution) for 24 hours at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... and goat anti-rat PE (50x stock concentration not reported, STAR73, Bio-Rad) diluted in PTxwH buffer ...
-
bioRxiv - Bioengineering 2023Quote: ... including Rat anti-F4/80 (dilution 1:50; MCA497G, BIO-RAD, Hercules, CA), Rabbit anti-iNOS (dilution 1:100 ...
-
bioRxiv - Cell Biology 2024Quote: ... or rat monoclonal anti-CD11b antibody (1:1000; BioRad, catalogue# MCA711G, lot# 0614) in blocking buffer overnight at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... Captured elution fraction from the Strep-Tactin-affinity purification were loaded onto a anion exchange column (UNO Q-6, Biorad) with a flowrate of 4 ml/min ...
-
bioRxiv - Neuroscience 2021Quote: ... the supernatant of the bacterial lysate was purified by affinity chromatography using an automated Profinia Protein Purification system (Bio-Rad), followed by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2020Quote: ... The labeled GyrA14-I491C was loaded onto a pre-equilibrated CcdB affinity column prepared using Affi-gel 10 (Bio-Rad), and incubated at 4°C for 4 hours on a rotary mixer to enable binding ...
-
bioRxiv - Molecular Biology 2022Quote: ... His-tagged recombination proteins were purified with cobalt-chelating affinity chromatography (Pierce) and desalted with HEPES buffer (pH 7.15) using the gel filtration column (Bio-Rad).
-
bioRxiv - Cell Biology 2022Quote: ... and affinity purified anti-HSP-90 antibodies by use of an MBP-HSP-90 antigen coupled to Affigel matrix (BioRad), using a procedure previously published (Benian et al. ...
-
bioRxiv - Molecular Biology 2022Quote: ... The BRM antigen was affinity-purified by IMAC using the NGC Medium-Pressure Liquid Chromatography System (Bio-Rad, Hercules, CA) and eluted in elution buffer (50mM sodium phosphate pH 8.0 ...
-
bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
-
bioRxiv - Cell Biology 2022Quote: ... the cell lysates or recombinant protein were loaded on a 4-20% Criterion gel (BioRad, 3450032) for SDS-PAGE and transferred to PVDF membrane (Millipore ...
-
bioRxiv - Immunology 2023Quote: ... Both sets of recombinant proteins were quantified using the Bradford protein quantification assay (Bio-Rad, UK).
-
bioRxiv - Evolutionary Biology 2021Quote: ... Following PCR amplification of pooled fragment libraries with a proofreading polymerase (Iproof; BioRad, Hercules CA), fragments ranging from 350 to 450 base pairs (bp ...
-
bioRxiv - Genetics 2019Quote: ... Desired fragments were amplified using a C1000 Touch Thermal Cycler (Bio-Rad, Inc., Hercules, CA).
-
bioRxiv - Biochemistry 2023Quote: ... and the size of the fragments evaluated by running a protein standard ladder (Bio-Rad). The Fab bands were cut and reduced by 10 mM TCEP at 37 °C ...
-
bioRxiv - Immunology 2023Quote: ... and the size of the fragments evaluated by running a protein standard ladder (Bio-rad). The Fab bands were cut and reduced by 10 mM TCEP at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... The enrichment of obtained chromatin fragments was estimated using qPCR (BioRad CFX96 Real-Time machine) using specific primers targeting specific and non-specific (negative control ...
-
bioRxiv - Developmental Biology 2021Quote: ... by immunoprecipitation of endogenous or endogenously Flag-tagged Piwi (eF-Piwi): The anti-Piwi antibody and Surebeads Protein A Magnetic bead (Bio-Rad, 1614013) were used to immunoprecipitated endogenous Piwi-piRNAs from witld type OSC ...
-
bioRxiv - Developmental Biology 2021Quote: ... to a LF (low fluorescence) PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Membranes were blocked in 5% BSA (VWR #0175) ...
-
bioRxiv - Synthetic Biology 2019Quote: ... 96-well Alpha™Unit cycling block and low-profile PCR stripes (Bio-Rad Laboratories GmbH, Muenchen, Germany) were utilized ...
-
bioRxiv - Plant Biology 2019Quote: ... and the proteins were compared with a Low range Pre-stained protein standard (Bio-Rad Laboratories, Hercules, CA). The purified recombinant protein was stored in storage buffer (20 mM HEPES (pH 7.5) ...
-
bioRxiv - Microbiology 2022Quote: ... The proteins were separated by SDS/PAGE gel and transferred to a PVDF low fluorescence membrane (Bio-Rad Laboratories Ltd ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in agarose insert buffer and mixed 1:1 with 2% low-melting agarose (Bio-Rad). Gel inserts were solidified at 4°C for overnight and were stored in agarose insert buffer until analysis ...
-
bioRxiv - Genetics 2019Quote: ... The resulting cDNA was PCR amplified and run on a 2% Certified Low Range Ultra Agarose (Bio-Rad) gel with subsequent extraction using the QIAquick® Gel Extraction Kit (Qiagen) ...
-
bioRxiv - Developmental Biology 2021Quote: ... to a LF (low fluorescence) PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Membranes were blocked in 5% BSA (VWR #0175) ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in 1 × PBS and spread on the surface of 1% low melting point agarose gel (Bio-Rad) prepared in conditioned medium ...
-
bioRxiv - Microbiology 2021Quote: ... CelA1 expression was measured using soFast EvaGreen Supermix with the Low ROX qPCR kit (Bio-Rad, CA, USA) and the CFX96 qPCR system (Bio-Rad ...
-
bioRxiv - Bioengineering 2022Quote: ... Separated proteins were transferred to low fluorescence PVDF membranes using the TransBlot Turbo (Bio-Rad, Hercules, CA, USA), and blocked in TBS-T (TBS with 0.1% Tween 20 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein was transferred to a low fluorescence PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% milk in TBS-T (140mM NaCl ...
-
bioRxiv - Genomics 2022Quote: ... using 0.5 × Tris-Borate-EDTA buffer and a 1% gel with low melting point agarose (Bio-Rad #1620137). Gels were stained with 5 μg/ml ethidium bromide in water after electrophoresis for 30 min ...
-
bioRxiv - Biochemistry 2021Quote: Primary screening for nanobody binding was achieved by a small-scale pulldown method in which Affi-gel 10 affinity resin (Bio-Rad) was bound to laforin and subsequently to each purified VHH clone34 ...
-
bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude) ...
-
bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
-
bioRxiv - Microbiology 2020Quote: 100 µL of well mixed anti-FLAG M2 Affinity Gel aliquots were loaded on columns (Micro Bio-Spin Columns, Bio-Rad) and washed two times with 1 mL of cell lysis buffer by gravity flow ...
-
bioRxiv - Molecular Biology 2023Quote: ... Western blot analysis was accomplished through SDS-PAGE of cell lysates or affinity-resin eluates in 1X laemmli loading-buffer (Bio-Rad) containing β-mercaptoethanol (1/10 in 4X buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... 20 minutes) and the clear lysate was purified on HisTrap HP 1 ml affinity column (Cytiva) by FPLC system (NGC™, BioRad). The protein was eluted from the column using a linear gradient (Elution buffer ...
-
bioRxiv - Biophysics 2023Quote: ... samples were processed using a Bio-Rad NGC Quest 10 Plus fast protein liquid chromatography (FPLC) apparatus with a 5 mL EconoFit Nuvia IMAC affinity column (Bio-Rad). To purify protein from the soluble fraction ...
-
bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
-
bioRxiv - Biophysics 2023Quote: ... Solubilized protein was cleared by ultracentrifugation at 46,400 g and purified by metal affinity chromatography using a Nickel-charged EconoFit Nuvia IMAC column (Bio-Rad, USA). Protein was eluted in 20 mM HEPES ...
-
bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
-
bioRxiv - Biochemistry 2022Quote: ... The refolded mature FAM237A was eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
-
bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
-
bioRxiv - Microbiology 2023Quote: ... Ni-NTA agarose with the bound His-SUMO-SigN was loaded on a Poly-Prep® Chromatography Column (Bio-Rad), washed with P2 buffer and subsequently with the P2 buffer with the 30 mM imidazole ...