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Citations for Bio-Rad :
151 - 200 of 370 citations for Recombinant Human Long Arg3 Insulin like Growth Factor I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... Resulting images were adjusted uniformly (‘High’ set to 40,000) to improve visibility of growth (Bio-Rad Image Lab™ Software). Figures utilizing these images (Supplementary Figs ...
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bioRxiv - Biophysics 2023Quote: ... and yeast cell growth was monitored by measuring the OD600 at 2 h increments in a microplate reader (Benchmark Plus, BioRad). The remainder of the 10 ml cultures was used to assess Pgp expression by Western blot analysis of microsomal membrane preparations using the monoclonal C219 anti-Pgp antibody.
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Immunology 2021Quote: ... Recombinant Bovine Interleukin-8 PBP039 and Goat anti Bovine Interleukin-8 Ab conjugated to biotin AHP2817B (all from Bio-Rad, protocol according to the manufacturer’s intructions).
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Biophysics 2023Quote: The monovalent streptavidin sample was kindly provided by the Howarth Lab (University of Oxford),23 and recombinant IgE Kappa (clone AbD18705_IgE, Cat#HCA190) was purchased from Bio-Rad Laboratories.
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bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Microbiology 2022Quote: ... from 22 people were quantified using a Bio-Plex Pro™ Human Inflammation 24-Plex Panel or a Bio-Plex Pro™ Human Th17 Cytokine Panel 15-Plex Panel (Bio-Rad, Hercules, CA, USA). Cytokines were omitted from further analyses if their concentration was close to the lower limit of detection in most samples ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... when the bacterial culture was within the logarithmic phase of growth measured by SmartSpec 3000 spectrophotometer (Bio-Rad, Hercules, CA, USA), the suspension was centrifuged at 1500 g for 10 min to harvest the pellet ...
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bioRxiv - Cell Biology 2023Quote: Cell growth in liquid media was monitored in a 96-well flat-bottomed plate using a microplate reader (iMark, Bio-Rad). Cells were grown to the mid-log phase and then diluted to OD600 = 0.1 ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated at 30°C and the growth was monitored by image scan using the device ChemiDoc MP (Bio-Rad) every 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: Cell cultures at the log phase of growth were collected and checked for cell number and viability using TC20 automated counter (Bio-Rad). Samples with >90 % viability were processed in order to prepare single cell suspensions and diluted into the target numbers plus the multiplet rate (1000 cells plus 0.8 % per library) ...
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bioRxiv - Biophysics 2024Quote: ... Growth media was exchanged after 24 hours and cells were checked for protein expression using a fluorescent cell imager (Bio-Rad). Cells were harvested after another 24 hours in cold PBS before purification ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were measured using the DCTM Protein Assay Kit I from Bio-Rad (cat # 5000111) by following the manufacturer’s protocol ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using The SYBR Green I Mastermix (Bio-Rad, US) and LightCycler480 thermocycler (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 μg DNase I treated RNA was reverse-transcribed using iScript cDNA Synthesis Kit (Bio-Rad) to prepare cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
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bioRxiv - Immunology 2023Quote: ... Bio-Plex Pro-Mouse Group I Cytokine 23-plex assay (M60009RDPD, Bio-Rad, Hercules, California, USA), Sirius red staining kit was purchased from Abcam (ab150681 ...
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bioRxiv - Developmental Biology 2023Quote: Lysates from early oocytes (stages I-II) were supplemented with 4x laemmli-sample buffer (Bio-Rad). 4 hpf embryos were deyolked in 55 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Human primers were designed by using Beacon Designer software (Bio-Rad). Quantitative PCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used: anti-human CD53 (MEM-53, Biorad), anti-human CD53-488 (MEM-53 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Bio-Plex Pro Human Cytokine VCAM-1 (BioRad, USA; 171B6022M) were used ...
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bioRxiv - Immunology 2020Quote: Human CTL were transfected using a GenePulser Xcell electroporation system (BioRad). 1x106 CTL (5days after restimulation therefore in expansion phase ...
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bioRxiv - Pathology 2023Quote: ... or mouse monoclonal antibodies against human C3d (Bio-Rad Laboratories, CA) and C4d (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... Primers/probes to detect human RPP30 (BioRad Custom Assay, Hercules, CA) were used as an endogenous reference in human samples ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: SDS-PAGE analysis of the recombinant TMPT2A (rTMPT2A) was performed on linear 12.5% polyacrylamide minigels in a Mini-Protean® Tetra cell system (BioRad, USA), as described by [22] ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... and the fractions containing the recombinant vaccine candidates were automatically collected using BioFrac™ Fraction Collector (Bio-Rad Laboratories, Hercules, USA) when the 280 nm absorbance was greater than 0.05 ...
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2021Quote: ... the plates were incubated at 37°C for 24 h in ambient air and growth was assessed at OD595 using microplate reader (iMark, Bio-Rad, Japan). The lowest concentration that completely prevented bacterial growth was categorized as MIC.
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bioRxiv - Molecular Biology 2020Quote: ... and reverse transcribed to make cDNA after DNase I treatment using iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative PCR was performed as previously reported (31–33) using SYBR Green I (Bio-Rad) on a Roche LightCycler 480 II ...
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bioRxiv - Microbiology 2020Quote: ... DENV gRNA copies were quantified by RT-qPCR using i-Taq one step universal probes kit (BioRad) and ZIKV and CHIKV gRNA copies with i-Taq one step universal sybr kit (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-MHC Class I RT1A (#MCA51G, 1:100, clone: OX-18, 1:100, Bio-Rad). Sequential imaging was performed on an Olympus FluoView confocal laser-scanning microscope (FV1200 ...
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bioRxiv - Cell Biology 2023Quote: ... The primary and secondary antibodies used were as follows: Collagen I (Bio-Rad 2150-1410, 1:300), α-SMA (Sigma A2547 ...
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bioRxiv - Immunology 2022Quote: ... Pre-designed human gene primers were purchased from Bio-Rad (supplemental Table). Human PPIA was amplified in parallel and used as the reference gene in quantification ...
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bioRxiv - Biochemistry 2020Quote: ... with goat anti-human IgG F(ab’)2-HRP (STAR126P, Bio-Rad) as the detection antibody.
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bioRxiv - Biophysics 2020Quote: ... and mouse anti-human CD34 (QBEND 10 clone, Ms IgG1, Bio-Rad). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Human primers for HLA-DRB1 and VCAM1 were purchased from Bio-Rad.
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in FACS buffer with Fc-block (Human Seroblock, Bio-Rad). These samples were then re-pelleted and suspended in a cocktail of fluorophore-conjugated primary antibodies (Supp ...
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bioRxiv - Immunology 2020Quote: ... and incubated with either FITC-conjugated Sheep Anti-Human C3 (BioRad AHP031F) at 1:500 or AF488-conjugated Mouse Anti-C5b-9 (ae11 ...
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Antiviral roles of interferon regulatory factor (IRF)-1, 3 and 7 against human coronavirus infectionbioRxiv - Microbiology 2022Quote: ... human IFN-γ and IFN-λ 1 were obtained from Bio-Rad, BD Pharmingen and R&D Systems respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... and G-CSF quantification using a human Bio-plex PRO assay (Biorad) following the manufacturer’s instructions and acquired with the Luminex200 instrument (Luminex).
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bioRxiv - Immunology 2023Quote: ... with human IgA positive and negative controls (Bio-Rad, Cat No. 12014775), Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... A human RPP30 copy number assay labeled with HEX (Bio-Rad, dHsaCP1000485) was used to measure total allelic copy numbers ...