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Citations for Bio-Rad :
351 - 400 of 654 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... For α-ACTIN-2 the secondary antibody Goat Anti-Mouse IgG (H + L)-HRP conjugate (1706516, Bio-rad) was used at a dilution of 1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with goat anti-rabbit IgG (H+L chains) HRP-conjugated secondary antibodies (Bio-Rad) and detected using SuperSignal West Pico ECL (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
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bioRxiv - Genomics 2023Quote: ... proteins were transferred (80 V; 2 h; 4°C) onto a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Membranes were air-dried ...
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bioRxiv - Plant Biology 2024Quote: ... followed by a 1 h incubation with anti-mouse IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature.
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: The recombinant protein of each vaccine candidate was purified under denaturing conditions using a biologic duoflow™ chromatography system (Bio-Rad Laboratories, Hercules, USA). A HisTrap™ HP column (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: Luminex profiles utilized the Human Inflammation Panel 1 37-plex assay kit (Bio-Rad) per the manufacturer’s protocol using the laboratory multianalyte profiling system (MAGPIX ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies used for SBA: Alexa 488 labeled mouse anti-human CD9 antibody (MCA469A488, Biorad), PE-labeled mouse anti human CD63 antibody (12-0639-42 ...
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bioRxiv - Cell Biology 2022Quote: ... along with Rhodamine-conjugated ɑ-GAPDH human Fab fragment (1:1000, Bio-Rad, 12004168). The blots were imaged on the ChemiDoc MP system and quantified using Image Lab software (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad, 171BL022M), Bio-Plex Pro Human Inflammation Panel 1 ...
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bioRxiv - Immunology 2023Quote: ... and chemokines dosage by Luminex using the 48-Plex pan-human cytokine kit (BioRad) according to the manufacturer procedure ...
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bioRxiv - Cell Biology 2022Quote: ... A FAM-labeled probe was used to target human STMN2 gene (dHsaCNS772300147, Bio-Rad) and a Hex-labeled probe was used to target mouse ApoB (dMmuCNS407594696 ...
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bioRxiv - Cancer Biology 2023Quote: ... media (for mouse or human tissue respectively) and 50 μL Alamar Blue (BioRad, BUF012B), with a corresponding media only control well ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti Human Protein Gene Product 9.5 (PGP9.5) (1:500, MCA4750GA mouse monoclonal, Biorad) for 36 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
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bioRxiv - Cancer Biology 2023Quote: ... for detecting mLIF or Bio-Plex Pro Human Cytokine LIF Set (Bio-Rad, 171B6011M) for detecting hLIF ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with the secondary antibody: the goat antirabbit IgG (H + L)-horseradish peroxidase conjugate (Bio-Rad). Blots were visualized by an Amersham Imager 600 gel scanner (GE HealthCare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... The reconstitution sample was nutated for 1 h at 4°C before addition of 0.2 g/mL SM2-BioBeads (BioRad), and the reconstitution sample was further nutated overnight at 4°C before removal of the biobeads ...
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bioRxiv - Synthetic Biology 2019Quote: ... Biorad) was applied for 1 h at RT and blots were developed with Carity™ Western ECL Substrate (Biorad).
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bioRxiv - Immunology 2021Quote: ... yeast (24 h post-induction) were counted using the TC20™ automated cell counter (Bio-Rad Hercules, CA, USA). Cells were centrifuged at 4,000 × G for 10 min and resuspended in PBS (Thermofisher) ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and exposed to X-ray film (Kodak) or the ChemiDoc Imaging system (Biorad) after incubation with Thermo Scientific Pierce ECL or Clarity ECL (Biorad).
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bioRxiv - Physiology 2020Quote: ... Total muscle phenylalanine was isolated using cation exchange chromatography (50W-8X, 200–400 mesh H+ form; Bio-Rad Laboratories). Amino acids were eluted in 8 mL of 2N ammonium hydroxide and dried under vacuum ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1 h at room temperature with peroxidase-conjugated anti-mouse (1:3000; BioRad, Hercules, CA) or anti-rabbit (1:3000 ...
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bioRxiv - Microbiology 2021Quote: ... the plates were sealed and incubated at 37°C for 2 h in a T100 Thermal Cycler (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
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bioRxiv - Microbiology 2020Quote: ... in 0.5×TBE buffer at 4 to 7 °C for 260 h using a CHEF Mapper System (Bio-Rad). Switching time was 1,200 to 4,800 s at 1.5 V/cm with an included angle of 120° ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed two times with TBST and then incubated with a 1:5,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed with TBST and then incubated with a 1:10,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... RMA-S (H-2b) was transfected with pcDNA3.1-HHD-A24 by electroporation (Gene Pulser Xcell, Bio-Rad, Hercules, CA), and cloned by the FACSAria II cell sorter (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-rabbit Igg (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6515,) were used as secondary antibodies ...
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bioRxiv - Synthetic Biology 2022Quote: ... The reaction was run for 7 h at 34 °C on a CFX96 real-time PCR detection system (Biorad) and monitored thanks to the SYBR Green II signal.
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bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were incubated for 18 to 24 h at 37°C and imaged in a ChemiDoc Imaging System (BioRad) to detect RFP and GFP fluorescence (Cy3 and Cy2 channels ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Bioengineering 2023Quote: ... The column was a BioRad HPX-87H (300 x 7.8 mm) with a BioRad Cation-H refill cartridge (30 x 4.6 mm) guard column (BioRad, USA) and the column oven was built in-house ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies (IgG(H+L)-HRP) were used at 1:10000 dilution for detection using a Versadoc system (Biorad).
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bioRxiv - Molecular Biology 2023Quote: ... Competent cells were adjusted to an OD620 of 2.0 in 100 µL and incubated with 10 to 15 µg of DNA on ice for 1 h before electroporation in a 0.1 cm electrode gap cuvette (1.5 kV, 400 Ω and 25 μF pulse; BioRad Gene Pulser Xcell electroporation system) ...
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bioRxiv - Microbiology 2024Quote: ... The HPLC column was an Aminex HPX-87H with Cation-H guard column 300×7.8mm (BioRad, Inc. Hercules, CA). The mobile phase was 0.02N H2SO4 with a flow rate of 0.500 mL/min ...
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bioRxiv - Molecular Biology 2024Quote: ... gel at 150 V for 2 h and transferred onto a PVDF membrane at 25 V for 2 h in transfer buffer (3.03g Tris–HCl, 14.4g glycine, 20% methanol) using a semi-dry transfer apparatus (Bio-Rad). After transfer ...
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bioRxiv - Molecular Biology 2019Quote: ... Measurement of serum cytokines was performed using multiplex ELISA (human 27-plex #m500kcaf0y, Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked by using PBS with 5% human AB serum (Bio-Rad, Germany) and 5% mouse serum (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... and human 18S genes using the iQ™ SYBER® Green Supermix (Bio-Rad, #1708880) with the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... For cytokine quantification Bio-Plex Pro Human Cytokine Screening Panel 48-plex (BioRad, USA; 12007283), Bio-Plex Pro Human Cytokine ICAM-1 (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... and the Bio-Plex Pro™ Human Inflammation Panel-1 Kit (37-Plex, Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Cell Biology 2020Quote: ... The SDS-PAGE gels were run at 100V for 1.5 h and transferred to a PVDF membrane by semidry electrophoretic transfer (Bio-Rad) for 45 min at 15 V ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...