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Citations for Bio-Rad :
301 - 350 of 654 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Pre-designed human gene primers were purchased from Bio-Rad (supplemental Table). Human PPIA was amplified in parallel and used as the reference gene in quantification ...
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bioRxiv - Biochemistry 2020Quote: ... with goat anti-human IgG F(ab’)2-HRP (STAR126P, Bio-Rad) as the detection antibody.
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bioRxiv - Biophysics 2020Quote: ... and mouse anti-human CD34 (QBEND 10 clone, Ms IgG1, Bio-Rad). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Human primers for HLA-DRB1 and VCAM1 were purchased from Bio-Rad.
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in FACS buffer with Fc-block (Human Seroblock, Bio-Rad). These samples were then re-pelleted and suspended in a cocktail of fluorophore-conjugated primary antibodies (Supp ...
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bioRxiv - Immunology 2020Quote: ... and incubated with either FITC-conjugated Sheep Anti-Human C3 (BioRad AHP031F) at 1:500 or AF488-conjugated Mouse Anti-C5b-9 (ae11 ...
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Antiviral roles of interferon regulatory factor (IRF)-1, 3 and 7 against human coronavirus infectionbioRxiv - Microbiology 2022Quote: ... human IFN-γ and IFN-λ 1 were obtained from Bio-Rad, BD Pharmingen and R&D Systems respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... and G-CSF quantification using a human Bio-plex PRO assay (Biorad) following the manufacturer’s instructions and acquired with the Luminex200 instrument (Luminex).
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bioRxiv - Immunology 2023Quote: ... with human IgA positive and negative controls (Bio-Rad, Cat No. 12014775), Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... A human RPP30 copy number assay labeled with HEX (Bio-Rad, dHsaCP1000485) was used to measure total allelic copy numbers ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Microbiology 2019Quote: ... and separated on an Aminex HPX-87H with Cation-H guard column (BioRad, Inc., Hercules, CA, USA) 300×7.8mm ...
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bioRxiv - Cell Biology 2021Quote: ... MYC tag (CST #2278) Secondary antibodies: Goat Anti Rabbit IgG (H+L) HRP Conjugate (BioRad #172-1019); Goat Anti Mouse IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary HRP- conjugated goat anti-mouse IgG(H+L) (1:10,000 dilution; Bio-Rad, USA), and then developed using the enhanced chemiluminescence procedure (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... by wet electroblotting for 1 h at 100 V using the Mini-PROTEAN Tetra system (Bio-Rad). Equal protein loading was assessed by amido black staining (0.02% (m/v ...
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bioRxiv - Biochemistry 2022Quote: ... gels were dried at 80 °C for 1 h in a gel dryer (Model 583, Bio-Rad), exposed to phosphorimager plates and signal acquired in Typhoon Trio™ Variable Mode Imager (GE Healthcare) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) was diluted at 1:5,000 in 1.5% BSA ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were then blocked for 1 h at 25 °C with 5% non-fat dry milk (BioRad) in TBST (50 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated with secondary antibodies Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad) diluted 1:5000 in TBST with 1% BSA for 1 h at RT ...
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bioRxiv - Biochemistry 2021Quote: ... After drying the gel (80 °C for 1.5 h) using a Model 583 Gel DRYER (Bio-Rad), it was exposed to a Phosphor Screen (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... h-Ras did not contain a HisTag and was purified on a Q-Sepharose column (Bio-Rad) in 20 mM Tris (pH 7.4) ...
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bioRxiv - Molecular Biology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L) secondary antibody was purchased from Bio-Rad (catalog number ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 1 h at 80 °C on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 h at room temperature in 5% skim milk (Catalog #170-6404, Bio-Rad). Primary antibody incubations were performed overnight at 4°C with antibodies diluted in TBS/0.1% Tween-20/5% BSA ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with the Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (Biorad, #1706515) diluted 1:2000 in BSA 1%/PBST ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody against rabbit IgG (Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (170-6515, Bio-Rad)) was used at a dilution of 1;25,000 for detection of Actin and Venus ...
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bioRxiv - Genetics 2023Quote: ... and orange G) and ran for 3 h at 100 V with a Variable Speed Pump (BioRad) to circulate buffer during the entire gel run.
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bioRxiv - Developmental Biology 2023Quote: ... and monitoring of mCherry expression after 6 and 22 h (ZOE fluorescent cell imager, Bio-Rad, 1450031).
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bioRxiv - Molecular Biology 2024Quote: ... and transferred onto PVDF membranes using a wet blot system for 1 h at 100 V (Biorad). Membranes were blocked with BSA and incubated with primary antibody overnight at 4° C ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h on a Gel Dryer 583 (Bio-Rad), exposed over night ...
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bioRxiv - Neuroscience 2024Quote: ... or at 100 V for 2 h at 4 °C using the wet transfer method (Bio-Rad Mini Trans-Blot Electrophoretic Cell 170-3930) ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Microbiology 2022Quote: ... and Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad, California, USA) were used to detect urinary cytokines ...
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bioRxiv - Microbiology 2022Quote: ... and a goat anti-human IgG conjugated to HRP (Bio-Rad, cat. 204005) was used as secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were analyzed using a human Th17 cell cytokine panel multiplex (BioRad 171AA001M). For flow cytometry ...
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bioRxiv - Bioengineering 2020Quote: ... or AlexaFluor647-conjugated mouse-anti-human CD120a (TNFR1) antibodies (clone H398, Bio-Rad). DAPI was used for dead cell exclusion.
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bioRxiv - Immunology 2023Quote: ... and Bio-Plex Pro Human IgA detection antibody (Bio-Rad, Cat No. 12014669). For both kits ...
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bioRxiv - Pathology 2023Quote: ... Immunoglobulins were analysed using a bio-plex pro human isotyping assay (Bio-Rad) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... for 16 h at 15 V using a Mini Trans-Blot® Cell (Art. No. 1703930, Bio-Rad) and 1x NuPAGE™ Transfer Buffer (Art ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 0.4 V cm−1 in a Mini-PROTEAN® Tetra Cell (Bio-Rad, CAD). His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at RT and detected using Clarity or Clarity Max Western ECL Blotting Substrate (Bio-Rad) and imaged (Amersham AI600) ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were incubated for 1 h on ice and finally measured using the S3e™ Cell Sorter (BioRad) apparatus ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 h at rtp with 5% (w/v) non-fat milk block (Bio-Rad) in 1x TBS (in mM ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were dried for 2 h at 80°C and analysed on a Personal Molecular Imager (Bio-Rad). For RNA stability assays ...
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bioRxiv - Microbiology 2019Quote: ... The hydrolysis halo area was measured after 24 h of incubation using the Image Lab™ Software (BIORAD). To correct for differences in colony growth ...
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bioRxiv - Neuroscience 2022Quote: Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6516,) and Goat anti-rabbit Igg (H+L ...