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Citations for Bio-Rad :
551 - 600 of 3204 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cDNA was generated from RNA using the iScript reverse transcription supermix for RT-qPCR (Bio-Rad, Hercules, CA USA) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted five-fold and quantitative PCR (RT-qPCR) was performed using iQ SYBR Green Supermix (Bio-Rad) per manufacturer’s protocol on a 7900HT Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was performed using SsoAdvanced Universal SYBR® Green Supermix (1725274; BioRad) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Each RT-qPCR reaction (10 μl) included 2 × iTaq™ Universal SYBR®Green Supermix (Bio-Rad, CA, USA), 500 nM forward and reverse primers (Supplementary File 1C) ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative Real rime PCR (RT-qPCR) analysis was performed on genes of interest (Supplementary Table 1) using SYBRGreen (BioRad). Sso Fast EvaGreen Supermix was performed to analyze gene expression on a BioRad CFX96 Real-Time System (BioRad ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR (RT-qPCR) was performed on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad) using 2× SYBR Green Real-Time PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was used as input for RT-qPCR using CFX-384 Touch Real-Time PCR System (Biorad), Power SYBR Green PCR MasterMix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Retrotranscription of RNA was performed using 3μg of total RNA in a 40μl final volume reaction mixture using iScript Advanced cDNA Synthesis Kit for RT-qPCR (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using the iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories, Hercules, CA, USA) with 2.0-20 ng input corneal RNA according to a manufacturer protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was diluted 1:5 and RT-qPCR was done using SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad) with PrimePCR Assays (CCDC40 ...
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bioRxiv - Molecular Biology 2023Quote: RT-and 3C-qPCR data was processed using the Bio-Rad CFX Manager™ Version 3.1 program (Bio-Rad) and analysed with Microsoft Excel ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (RT-qPCR) assays were performed on a CFX96 real-time PCR system (Bio-Rad, Hercules, CA, USA) using SsoAdvanced Universal SYBR Green Supermix (12 μL final mix per reaction ...
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bioRxiv - Pathology 2023Quote: ... The RT-qPCR was performed using a BIO-RAD C1000 Thermal cycler according to a standardized protocol (BIO-RAD), with Roche SYBR green ...
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bioRxiv - Molecular Biology 2024Quote: We quantified selenon transcripts in zebrafish using PrimePCR multiplex real time quantitative PCR (RT-qPCR) system by Bio-Rad. We extracted and purified total RNA from cells and zebrafish using Trizol LS reagent (Ambion ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (RT-qPCR) assays were performed on a CFX96 real-time PCR system (Bio-Rad, Hercules, CA, USA) using SsoAdvanced Universal SYBR Green Supermix (12 μL final mix per reaction ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using the iScript™ cDNA Synthesis Kit and iTaq Universal SYBR Green Supermix (Bio-Rad) and run on a Biorad CFX384 real-time system (C1000 Touch Thermal Cycler) ...
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bioRxiv - Microbiology 2024Quote: ... and then lysed by a 5-minute incubation with with 50 uL iScript RT-qPCR Sample Preparation Reagent (BioRad) (64) ...
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bioRxiv - Immunology 2023Quote: ... the RNA was converted to cDNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-rad, Hercules, CA, #1708840) or Sensifast cDNA Synthesis kit (Meridian Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was transcribed into cDNA using the iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, Hercules, CA). Real time PCR (Polymerase Chain Reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... SYBR Green RT-qPCR was performed using 2uL of cDNA with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 3 machine with regular settings (95°C for 10 minutes and 40 cycles at 95°C for 15 seconds and 60°C for 60 seconds) ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT reactions were diluted 2-fold and qPCR was performed in duplicate with SsoAdvanced SYBR Green Supermix (Bio-Rad) according to manufacturer’s protocols with qPCR_FWD and qPCR_REV primers from IDT (see below) ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time quantitative PCR analyses were performed in triplicate using the Fast Eva Green qPCR Master Mix (BioRad). GAPDH was used as an internal control for qRT-PCR analyses.
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bioRxiv - Microbiology 2021Quote: qPCR was performed using SSoAdvanced SYBR master mix and the CFX96 Touch Real-Time PCR system (Bio-Rad). Excision frequencies were calculated as the number of copies of the empty chromosomal attachment sites (as indicated by the Cp values measured through qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... qPCR was performed using SYBR Green Master Mix with the CFX96 Touch Real-Time PCR Detection System (BioRad). Cycle threshold (C(T) ...
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bioRxiv - Neuroscience 2022Quote: ... Real-Time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad, Cat. 1725124) on a SFX96 Real-time system (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: qPCR was performed using SsoAdvanced SYBR master mix and the CFX96 Touch Real- Time PCR system (Bio-Rad). Excision frequencies were calculated as the number of copies of the empty chromosomal attachment sites (as indicated by the Cp values measured through qPCR ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was done using SSoAdvanced SYBR master mix and CFX96 Touch Real-Time PCR detection system (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: ... followed by 19 µL of a qPCR master mix containing iTaq Universal SYBR Green Supermix (Bio-Rad 1725121), 500 nM forward primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA samples (10 ng/μl) were mixed with qPCR master mix with iQ SYBR Green supermix (Biorad, USA) with primers (10 μM ...
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bioRxiv - Microbiology 2024Quote: ... and gyrA (control) gene-specific qPCR F/R primers in a 1X SYBR green master mix (Bio-Rad). Threshold and baseline parameters were kept consistent for experiments performed on different days ...
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bioRxiv - Evolutionary Biology 2021Quote: ... gene expression was normalized with the housekeeping gene Act5c (primers: 5’ –gcgcccttactctttcacca-3’ and 5’ - atgtcacggacgatttcacg-3’. We performed the RT-qPCR analysis with SYBR Green (BioRad) or qPCRBIO SyGreen Blue Mix LO-ROX (PCR BIOSYSTEMS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cdk6 and gli3 in the developing fins were quantified through RT-qPCR (CFX96 real-time C1000 thermal cycler, Bio-Rad) and normalized to the expression level of the housekeeping gene ef1a (Extended Data Table1) ...
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bioRxiv - Cell Biology 2019Quote: ... Bio-Rad Sso Advanced Universal SYBR Green Supermix was used for RT-qPCR and data was analysed by Bio-Rad CFX Manager ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was diluted to 10 ng/µland 2 µl was used in each RT-qPCR reaction with 10 µl iTAQ Universal SYBR green supermix (BioRad) and 400 nM primers ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was analyzed on Tape Station analyzer and cDNA was generated using iScript Reverse Transcription Supermix for RT-qPCR (Biorad). qPCR was run using qPCR SYBR Green Assay with mouse BCL2 PrimePCR assay (Biorad ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 0.5 µg of total RNA using a RevertAid Premium Kit (Fermentas) and subjected to RT-qPCR using iTaq Universal SYBR Green Supermix (BioRad) and 10 ng of cDNA per reaction ...
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bioRxiv - Bioengineering 2020Quote: ... Quantitative real time RT-PCR (QPCR) were performed on the Opticon Continuous Fluorescence System (Bio-Rad Laboratories, Inc., Hercules, CA) using the QuantiTect SYBR Green RT-PCR kit (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... RT-qPCR was performed on a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA), by 40 cycles of denaturation at 95 °C for 30 s ...
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bioRxiv - Cancer Biology 2022Quote: ... and RT-qPCR was performed using iQ SYBR Green Supermix and the iCycler Real-time PCR detection system (Bio-Rad). Primer nucleotide sequences (Table S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR was performed using iQ SYBR green super mix in the CFX-96 thermal cycler (Bio-Rad, CA, USA). Amplification was carried out for 35 cycles where each cycle involved initial denaturation 95°C for 30 sec ...
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bioRxiv - Microbiology 2022Quote: cDNA was prepared for RT-qPCR by reverse transcribing one microgram of the extracted RNA using iScript Reverse Transcription Supermix (BioRad). qPCR was performed using iTaq-Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Plant Biology 2021Quote: ... was used for RT-qPCR using three technical replicates per reaction in a CFX96 Touch Real-Time PCR Detection System (Biorad). Primers are described in Table S4 ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting cDNAs were used as a template for RT-qPCR using a CFX96 Real-Time PCR Detection System (Biorad) with a SYBR Green master mix (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative real time RT-PCR (QPCR) was performed on the Opticon Continuous Fluorescence System (Bio-Rad Laboratories, Inc., Hercules, CA) using the QuantiTect SYBR Green RT-PCR kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... EdU-DNA as well as input DNA was quantified by RT-qPCR using the IQ SYBR green Supermix (Bio-Rad) and a CFX96 Fast Real-Time system (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesised from RNA (500ng) using iScript™ Reverse Transcription Supermix for RT-qPCR (BIO-RAD, Hercules, CA, USA). The cDNA was diluted 10-times for quantitative PCR (qRT-PCR ...
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bioRxiv - Pathology 2020Quote: ... 0.5 μg of total RNA was reverse-transcribed using the iScript Reverse Transcription Supermix for RT-qPCR (BioRad, cat# 1708841). SYBR Green reactions using the SYBR Green qPCR supermix (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 ng of cDNA were then subjected to PCR amplification on an RT-qPCR system using the CFX96 Real Time PCR detection system (Biorad). The SYBR® Green SuperMix for iQTM (Quanta Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... RT+ and RT-reverse transcription reactions were diluted 67-fold in RNase/DNase free water and RT-qPCR was performed on the CFX96 Real Time System (BioRad) using GoTaq qPCR Master Mix (Promega) ...