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Citations for Bio-Rad :
301 - 350 of 459 citations for L Alanine N T Boc 13C3 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2022Quote: ... DNA/protein complexes were transferred to a Hybond-N+ positively charged nylon membrane (Amersham Pharmacia Biotech) using an electroblot transfer system (Bio-Rad) and cross-linked with short-wave UV light in a GS gene linker UV chamber (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... with 10 μg of plasmid DNA expressing LpAsp-GFP or LpCht-GFP as well as pTrex-n-eGFP using a Gene Pulser X cell electroporator (Bio-Rad) and cuvette (2 mm gap) ...
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bioRxiv - Molecular Biology 2023Quote: ... and A6p (Table S1) were run on agarose gels and purified using Freeze ‘N Squeeze DNA gel extraction spin columns (Bio-Rad). For manual sequencing ...
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified RNA was separate on a 1.2% agarose-formaldehyde denaturing gel and was then transferred to a nylon membrane (Hybond-N+, Cytvia) in 10X SSC buffer using a model 785 vacuum blotter (Bio-Rad). RNA was cross-linked to the membrane using an ultraviolet (UV ...
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bioRxiv - Microbiology 2023Quote: ... targeting SARS-CoV-2 gRNA and N protein sgRNA were used for the quantification by RT-qPCR using iTaq Universal SYBER Green Supermix (Bio-Rad) and normalised to β-actin using 2-ΔΔCT.
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Genomics 2024Quote: ... Samples were then run on a 2% agarose gel with 1x SYBR Safe for 2 hours at 120 V before gel extraction with a Freeze ‘N Squeeze column (Bio-Rad). After extraction ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Biochemistry 2023Quote: ... to saturate the N-terminal metal binding site,10 prior to buffer exchange using a centrifugal buffere exchange device (Bio-Spin, Bio-Rad) into 200 mM ammonium acetate supplemented with 2 x CMC of C10E5 ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant extract containing l-Opa1 was loaded onto a Ni-NTA column (Biorad, CA, USA), washed with 50 mM sodium phosphate ...
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bioRxiv - Neuroscience 2021Quote: ... or Goat Anti-Rabbit IgG (H L)-HRP Conjugate (1:1000; Bio-Rad; Catalog # 172-1019) in TBS-T for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP-conjugated Goat Anti-Rabbit IgG (H+L) (1:1000, 170-6515, Bio-Rad, CA, USA) or Goat-anti-Mouse IgG (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... and 1: 25000 diluted secondary HRP conjugated Goat anti-mouse IgG (H+L) (BioRad, Mississauga, Canada). The blot was developed using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... After 5 washes with PBS1X-Tween 0,05% an anti-mouse IgG (H + L)-HRP Conjugate (Biorad) diluted 1:2000 in PBS1X-Tween 0,05% was added for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used were goat α-mouse IgG(H+L)-HRP (no. 170-6516, Bio-Rad) and goat α-rabbit IgG(H+L)-HRP (no ...
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bioRxiv - Molecular Biology 2023Quote: ... goat anti-rabbit IgG (H + L)-HRP conjugate (170-6515, Bio-Rad, CA, USA; 1:2000), and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Cell Biology 2024Quote: ... and secondary antibody goat anti-rabbit IgG (H+L)-HRP-conjugate (1:10,000 dilution, Bio-Rad). Image lab (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... followed by 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 3% milk in PBS-T and developed as described above.
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Genomics 2019Quote: ... Total HIV-1 DNA content of CD4 T-cell DNA isolates was measured by digital droplet PCR (ddPCR, QX200 platform, Bio-Rad, Temse, Belgium), as described by Rutsaert et al.56 The DNA was subjected to a restriction digest with EcoRI (Promega ...
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Immunology 2021Quote: ... Purified CD8+ T cells were counted and cell diameter measured using a Moxi Z mini (Orflo, MXZ001) or a TC20 (Bio-Rad, 145-0101) automated counter ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were washed five times in TBS-T before detection with chemiluminescent HRP substrate (Immobilon, P90720) and imaged on a ChemiDoc™ XRS+ system (Bio-Rad, 1708265).
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bioRxiv - Cancer Biology 2021Quote: ... NRF2 (CST, 12721S), KEAP1 (CST, 8047S), PRDX1 (CST, 8499S), GSTO1 (Thermo, PA5-83383)] in 5% BSA in TBS-T (Bio-Rad, 170-6435). Following incubation with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed again three times with TBS-T followed by the addition of primary antibodies (neat antivenom and normal horse IgG [BioRad] as negative control) in duplicate ...
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bioRxiv - Immunology 2024Quote: ... the fractions were eluted with 0.25 M NaCl and biologically active fractions were further analyzed on a hydrophobic interaction column (Macro-Prep® t-Butyl HIC Resin, Bio-Rad 1580090). Three fractions eluted with buffers with pH=7 ...
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bioRxiv - Plant Biology 2024Quote: ... the membrane was washed twice with the 1x TBS-T buffer and then incubated with the Clarity Western ECL Substrate solution (Bio-Rad, California, USA). Signal detection of the membranes was performed by the Chemi-Doc Imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes underwent three times with PBS-T and were then subjected to incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, CA, USA) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples (n = 80 embryos / lane) were run on a precast SDS-PAGE gel (4-20% MP TGX Stain-Free Gel, Bio-Rad 4568093), with a Precision Plus Protein WesternC Stds ladder (Bio-Rad 1610376) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μL 5 N NaOH solution was added to and the absorbance was read at 405 nm with a reference filter of 620 nm by iMark Microplate Reader S/N 20255 (Bio-Rad, USA).
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bioRxiv - Synthetic Biology 2019Quote: ... at which point waste acrylamide was separated from the aqueous slats solution with Freeze N’ Squeeze spin columns (Bio-Rad, 732-6166). The DNA slats were further purified by precipitation in isopropanol ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from cardiac cultures after 4 days on either soft or stiff PDMS (n=4) using the AurumTM Total RNA Mini Kit (Bio-Rad Laboratories). Libraries were constructed by the Purdue Genomics Core Facilities according to standard protocols using TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Target bands were excised on a blue light transilluminator and purified with a Quantum Prep® Freeze ‘N Squeeze spin column (Bio-Rad). The eluate was further purified with a magnetic bead clean-up (Agencourt® AMPure® XP ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was slot blotted onto a Hybond N+ membrane with the help of a dot-blot apparatus (Bio-Rad, 170-6545) and subsequently hybridized with a biotin-labeled (TTAGGG)3 probe synthesized by Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was determined by Bradford measurement (64) according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Physiology 2023Quote: ... in duplicates in 384-qPCR plates (n = 5 plates) and qPCR were performed with a Biorad instrument (CFX-384, Biorad, Hercules, USA). We used Recombination Activating Gene 1 (RAG1 ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Cell Biology 2021Quote: ... MYC tag (CST #2278) Secondary antibodies: Goat Anti Rabbit IgG (H+L) HRP Conjugate (BioRad #172-1019); Goat Anti Mouse IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary HRP- conjugated goat anti-mouse IgG(H+L) (1:10,000 dilution; Bio-Rad, USA), and then developed using the enhanced chemiluminescence procedure (Pierce ...
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bioRxiv - Synthetic Biology 2020Quote: ... The horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) was diluted at 1:5,000 in 1.5% BSA ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated with secondary antibodies Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad) diluted 1:5000 in TBST with 1% BSA for 1 h at RT ...
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bioRxiv - Immunology 2022Quote: ... SDS 10g/l) and transferred onto a PVDF membrane with the Trans-Blot Turbo Transfer System (BIORAD). The membrane was blocked using a blocking solution (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L) secondary antibody was purchased from Bio-Rad (catalog number ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with the Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (Biorad, #1706515) diluted 1:2000 in BSA 1%/PBST ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody against rabbit IgG (Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (170-6515, Bio-Rad)) was used at a dilution of 1;25,000 for detection of Actin and Venus ...
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bioRxiv - Systems Biology 2023Quote: ... resolved in a 4-12% Invitrogen B-T gel using MOPS buffer and transferred onto a polyvinylidene difluoride (PVDF) membrane using BioRad transblot (Bio-Rad, Hercules, CA, USA). The membrane was blocked with 5% skim milk ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...