Labshake search
Citations for Bio-Rad :
201 - 250 of 459 citations for L Alanine N T Boc 13C3 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h RT with the secondary antibody in milk PBS-T (Goat anti-mouse IgG HRP conjugate (170-6516; BioRad); Goat anti-rabbit IgG HRP conjugate (170-6515 ...
-
bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified goat α-rabbit or goat α-mouse IgG conjugated to alkaline phosphatase ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed in PBS-T and developed for 2 minutes with ClarityTM Western ECL Substrate (Bio-Rad, CA, USA). Imaging was performed using a ChemiDocTM MP Imaging System with Image LabTM software (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
-
bioRxiv - Plant Biology 2021Quote: ... in TBS-T (TBS supplemented with 0.1% (v/v) Tween-20) and 1% (w/v) Blotting-Grade Blocker (Bio-Rad) at 4°C overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were washed 3 times for 5 minutes with TBS-T prior to incubation in ECL reagent for 1 minute and imaging using a Chemidoc MP (BioRad). Images acquired were analyzed using Image Lab software (BioRad).
-
bioRxiv - Cell Biology 2019Quote: ... PVDF membranes were blocked with 5% non-fat dry milk (wt/vol) in TBS-T buffer (0.1% vol/vol of Tween-20 in TBS buffer solution, #1706435 Bio-Rad) for 1 h at room temperature ...
-
bioRxiv - Genetics 2020Quote: ... The membrane was washes in TBS-T three times and incubated 5 min with Clarity Western ECL Substrate (#1705061, Biorad) in order to detect proteins immunoblotted signals at Alliance LD4 (UVItec Cambridge ...
-
bioRxiv - Molecular Biology 2019Quote: ... The slide was washed in TBS-T (4x 5 min) and visualized using SuperSignal West Pico chemiluminescent substrate (Pierce) and a ChemiDoc MP System (BioRad). Signal intensity normalization was performed using Array Analyze Software (Active Motif).
-
bioRxiv - Physiology 2021Quote: ... Membranes were washed with 4 x 15 min TBS-T washes prior to incubation with secondary antibody (goat anti-rabbit-HRP diluted 1:10,000, Bio-Rad) for 1 h on a shaker at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... The blots were then washed 3x with TBS-T for 10 min each and incubated with the horseradish peroxidase (HRP) conjugated specific secondary antibodies (1:20,000, Bio-Rad) for 1 h at RT ...
-
bioRxiv - Biochemistry 2021Quote: ... Staining was removed by washing the membrane in TBS-T and the membrane was blocked for 20 min using 1% Casein in TBS (BioRad). To detect PAR chains with incorporated 6-Biotin-17-NAD+ (Biolog) ...
-
bioRxiv - Biochemistry 2021Quote: ... 2.5×106 Jurkat T cells were electroporated with 2 μg minigene constructs using 220 V and 1,000 mA (Gene pulser X,Bio-Rad). After 24 h incubation ...
-
bioRxiv - Molecular Biology 2021Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h at RT with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad). After the same washing procedure ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBS-T 3 times after each step and finally imaged on the ChemiDoc MP Imaging System (Biorad).
-
bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3 × 5min TBS-T and 2 × 5 min TBS) before being imaged on the ChemiDoc MP Imaging System (Biorad). Subsequently ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The membrane was washed for 5 min with TBS-T and then imaged using a ChemiDoc XRS+System (Bio-Rad). For chemiluminescence-based detection of immunoblots ...
-
bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked for 1 hr in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4°C with antibodies listed in Table S1 in 5% BSA in TBS-T ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane was then washed three times with TBS-T for 5 min each before developing using Clarity MaxTM Western ECL Substrate (1705062; BioRad) and imaged using a BioRad ChemiDoc XRS+.
-
bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubated with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad) or Goat anti-mouse IgG HRP conjugate (170-6516 ...
-
bioRxiv - Genetics 2022Quote: ... We washed the membrane 3× 5 minutes in TBS-T and 1× 5 minutes TBS before imaging (Bio-Rad ChemiDoc).
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... secondary antibodies to the membrane for 1 h in the presence of 5% milk followed by four washes in TBS-T and developing with ECL substrate (1705061; BioRad). Blot signal was captured using the same BioRad ChemiDoc™ Touch Gel Imager ...
-
A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3x with 10 mL of TBS-T and developed with Clarity Max ECL substrate (Bio-Rad). Western blot images were taken with an Azure Biosystems C400 imager.
-
bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4 °C with antibodies listed in Supplementary Table 2 in 5% BSA in TBS-T ...
-
bioRxiv - Molecular Biology 2021Quote: ... the entire samples were applied to Hybond-N using a Bio-Dot® Microfiltration Apparatus (Bio-Rad Laboratories, Inc.). After UV crosslinking ...
-
bioRxiv - Cell Biology 2021Quote: ... The N-ter (pI 5.5) and C-ter (pI 9.9) peptides were conjugated to Affi-Gel-10 (Bio-rad) agarose beads for affinity-purification ...
-
bioRxiv - Bioengineering 2024Quote: ... Structures were also evaluated by TEM to confirm structural stability following gel purifification using Freeze ’N Squeeze (Bio-Rad) gel extraction columns.
-
bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized using ECL-based autoradiography film system (Super RX-N, Fujifilm) or ChemiDocTM Imaging system (BioRad) and analyzed using Gel Analyzer plugin from ImageJ (Fiji) ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mg/L cefotaxime supplemented Drigalski agar plates (Bio-Rad, Marne-la- Coquette, France) and Cetrimide media on which was deposited a disc of ceftazidime in the 2nd quadrant (bioMérieux) ...
-
bioRxiv - Molecular Biology 2019Quote: ... and goat anti-mouse IgG H&L HRP conjugate (Bio-Rad, Hercules, CA, USA) was used as the secondary antibody at a dilution of 1:2000 ...
-
bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected with Goat Anti-mouse-IgG (H+L)-HRP Conjugated (BioRad) and bound conjugate was detected using TMB (Invitrogen/Life technologies ...
-
bioRxiv - Microbiology 2019Quote: ... goat anti-mouse IgG (H+L)-HRP conjugate (1:5,000 dilution, Bio-Rad Laboratories). After three washes with TBS-T ...
-
bioRxiv - Molecular Biology 2022Quote: ... and HRP-conjugated goat anti-rabbit IgG (H+L) was purchased from Bio-Rad (catalog number ...
-
bioRxiv - Molecular Biology 2023Quote: ... and goat anti-rabbit IgG (H+L)-HRP Conjugate antibody (Bio-Rad #170-6515) was used for 2 hours at a dilution rate of 1:3000 in blocking buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... secondary goat anti-mouse IgG (H+L)-HRP Conjugate antibody (Bio-Rad #170-6516) and goat anti-rabbit IgG (H+L)-HRP Conjugate antibody (Bio-Rad #170-6515 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used are Goat anti Mouse IgG (H/L): HRP (Bio-Rad, STAR207P) and Goat anti Mouse IgG (H/L) ...
-
bioRxiv - Physiology 2023Quote: ... Secondary antibodies used were goat anti-mouse IgG (H+L)-HRP conjugate (Biorad 1706516), AffiniPure goat anti-rabbit IgG (H+L)-HRP conjugate (Jackson ImmunoResearch 111035144).
-
bioRxiv - Plant Biology 2024Quote: ... and the secondary antibody goat Anti-Mouse IgG (H + L)-HRP conjugate (Bio-Rad) was used at a dilution of 1:5000 ...
-
bioRxiv - Cell Biology 2023Quote: ... and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516, Biorad, 1:10000). Antibody binding was detected by the enhanced chemiluminescence system (Thermo Fischer Scientific) ...
-
bioRxiv - Genetics 2024Quote: ... goat anti-mouse IgG (H+L) HRP conjugate (1:10000, Bio-Rad Cat. # 1706516).
-
bioRxiv - Immunology 2021Quote: ... Macrophages and T cells in aortic root were stained by incubating aortic root cross-sections with the primary antibodies to CD68 (Bio-Rad) and CD3 (Abcam) ...
-
bioRxiv - Microbiology 2021Quote: ... the membranes were washed 3 times with 1 x TBS-T and antibody binding was detected in a BioRad Versa Doc System with the Quantity One software (BioRad, Germany) by chemiluminescence using the Clarity Western ECL Substrate (BioRad ...
-
bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
-
bioRxiv - Biochemistry 2021Quote: ... The membrane was washed three times with PBS-T (15 min, RT) and chemiluminescence was detected after 10 min incubation with freshly prepared Clarity Western ECL Substrate (Bio-Rad) with a LAS-4000 gel scanning station (Fujitsu Life Sciences ...
-
bioRxiv - Neuroscience 2021Quote: ... USA) that were diluted 1/5000 in TBS-T and revealed by enhanced chemiluminescence plus (ECL) solution (Biorad Laboratories, ON, Canada). β-actin was used to ensure equal protein loading ...
-
bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed again for 3 × 5-min in TBS-T before being immersed for 5 min under gentle agitation at room temperature in Clarity ECL detection substrate (Bio-rad Laboratories Pty Ltd ...