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Citations for Bio-Rad :
151 - 200 of 5856 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The resin was then transferred to an open column (Bio-Rad) where it was washed with binding buffer followed by washing buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A temperature gradient of 25 to 70°C (heating rate 1°C per min) was applied using a MJ minicycler (Bio-Rad, Hercules, CA, USA). All experiments were performed triplicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... touchdowns from 56 °C to 50°C for 1 min and 50 cycles with 95 °C for 30 s and 50 °C for 1 min in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... followed by 40 cycles (95°C for 5 seconds, 60°C for 40 seconds) on a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA). The housekeeping gene GAPDH (PY17X_1330200 ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C in a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked in 3% non-fat dry milk in TBS-T buffer for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... and cooled to 4°C using a thermal cycler (Biorad). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... hold at 4°C in a C1000 Thermal Cycler (Biorad). We then performed RT-qPCR for gene expression quantification of the following genes ...
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bioRxiv - Microbiology 2021Quote: ... at 8°C in a MyCyclerTM thermal cycler (Bio-Rad). At the indicated intervals ...
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bioRxiv - Microbiology 2019Quote: ... at 37°C in a S1000 thermal cycler (Bio-Rad). The starting volume of each reaction was 100 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... Precision Plus Protein Western C Standards (Bio-Rad, 161-0376) were used to determine molecular weights ...
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bioRxiv - Neuroscience 2024Quote: ... held at 4 °C (C1000 Touch Thermal Cycler; Bio-Rad). Preamplified cDNA was immediately diluted 1:5 with 20 uL DNA Suspension Buffer and stored at –20 °C until use.
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bioRxiv - Microbiology 2023Quote: ... and held at 4 °C in a thermocycler (Bio-Rad). The newly synthesized 20 µL cDNA reactions were diluted 1:5 with 80 µL sterile ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Plant Biology 2023Quote: ... TGX FastCast Acrylamide kit (12%, Bio-Rad, CA, USA) was used for SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... The qRT-PCR was performed in a 2-step amplification with 40 cycles of 95 °C for 30 s and 55 °C for 30s using a Bio-Rad IQ thermocycler (Bio-Rad Laboratories Inc. Hercules, CA). Each reaction was loaded on a 384-wells standard qPCR plate and ran in triplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... The enzymes were subsequently incubated at temperatures from 30 °C to 54 °C for one hour using a PCR thermocycler machine (Bio-Rad Laboratories, Hercules, CA, USA), followed by cooling for 10 minutes at 4 °C ...
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bioRxiv - Systems Biology 2021Quote: ... and the PDMS pad for medium perfusion was attached on the coverslip via a square-frame two-sided seal (Frame-Seal™ Incubation Chambers, Bio-rad). We immediately filled the device with the fresh medium and connected it to a syringe pump on the microscope stage.
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bioRxiv - Cell Biology 2019Quote: ... The resin was loaded into an open column (Bio-Rad, #731-1550) and washed first with ice-cold 20 ml binding buffer ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Biophysics 2020Quote: ... using a 9 × 9 mm2 frame seal (SLF0201, Biorad). The chamber was plasma-treated to improve wettability ...
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bioRxiv - Plant Biology 2020Quote: ... Mitochondrial protein was quantified using DC Protein Assay (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification conditions were initial denaturation at 95°C for 10 min followed by 40 cycles of 95°C for 10 s and annealing at 58-65 °C for 30 s (Bio-Rad CFX Connect Real-Time PCR) followed by melt peak analysis ...
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bioRxiv - Immunology 2021Quote: ... Settings for analysis using a C-1000 CFX96 machine (Bio-Rad) were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... overnight at 37 °C in a G1000TM Thermal cycler (BIO-RAD) to digest trehalose to produce free glucose ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then to 97°C for 8 min in a thermocycler (BioRad). We centrifuged the samples and froze the supernatant for further use.
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-cytochrome c (AHP2302, Bio-Rad Laboratories, 1:500), and mouse monoclonal anti-β-actin (A5441-.2ML ...
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bioRxiv - Microbiology 2019Quote: ... 72°C,20"]x45 using SSO Advanced SYBR Green Supermix (BioRad), and 300 nM Tox9 and 11 primers listed in Table S1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... at 75°C for 10 minutes (T100 Thermal Cycler, Bio-Rad). The denatured proteins were pelleted through centrifugation at 3,200 g for 2 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... at 75°C for 10 minutes (T100 Thermal Cycler, Bio-Rad). The denatured proteins were pelleted through centrifugation at 3,200g for 2 minutes.
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bioRxiv - Physiology 2023Quote: ... and transferred (100V, 1h, 4°C) onto PVDF membranes (Bio-Rad). Blots were blocked in buffer containing 5% BSA (Cat#A9418 ...
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bioRxiv - Biochemistry 2023Quote: ... 59 and 61°C in an S1000 thermal cycler (Bio-Rad). For CETSA with dose-response ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed using EBC buffer (Bio-Rad Cat. # C-1410) supplemented with protease EDTA-free (Cat # ...
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bioRxiv - Neuroscience 2023Quote: ... and 72°C 15 s using a PCR machine (T100, Biorad). PCR primer pairs used were 5’-TCCCAGCCTGAAAGCCACAG-3’ and 5’-ATGCTGCTGGGATTCTCTCCC-3’ for Pet1 ...
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bioRxiv - Genetics 2023Quote: ... Membranes were incubated overnight at 4°C with STAR5B Streptavidin:HRP (BioRad) at a 1:15,000 dilution in 1x PBST ...
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Bioaugmented sand filter columns provide stable removal of pesticide residue from membrane retentatebioRxiv - Microbiology 2020Quote: ... 16S rRNA gene copy numbers were here automatically calculated from the 16S rRNA gene copies of the standard curve by Bio-Rad CFX manager 3.1 ...
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bioRxiv - Microbiology 2023Quote: ... Amplification of 16S rRNA and fcrX cDNA was made using SsoFast EvaGreen Supermix 2X kit (Bio-Rad, France) on a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were stored at −80°C until cDNA synthesis using the iScript™ gDNA Clear cDNA Synthesis Kit according to the manufacture’s guidelines (BIO-RAD). A qPCR-based assay was used to detect and quantify PfCSP transcripts (Kefi et al. ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Immunology 2019Quote: ... The readings were acquired using a microplate reader (Bio-Rad iMARK) at 450 nm.
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bioRxiv - Cell Biology 2023Quote: ... supernatant was applied to an open column with Ni-IMAC resin (BIO-RAD). The column was washed with wash buffer (50 mM Tris-HCl pH8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Frame-Seal slide chambers (9 × 9 mm2, Biorad, Hercules, CA) were then secured to a coverslip ...
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bioRxiv - Bioengineering 2021Quote: ... which was frame-seal incubation chamber (BIO-RAD SLF0201, USA) which is illustrated in Figure 8a or 12-mL centrifuge tube (Corning #430766 ...
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bioRxiv - Biophysics 2022Quote: ... Frame-seal slide chambers (9 × 9 mm, Bio-rad, USA) were affixed to the glass coverslips ...
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bioRxiv - Microbiology 2019Quote: ... 98°C for 10 min) with C1000 Touch thermal cycler (Bio-Rad). Fluorescent signal was detected using QX200 Droplet Reader (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated (4°C, overnight) with 5 % milk powder (BioRad) in TBST ...