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Citations for Bio-Rad :
201 - 250 of 314 citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... coli and plasmid DNA were electroporated in 1 mm cuvettes at 1.8 kV using the MicroPulser electroporator (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... and transformed by electroporation with 100 ng of the temperature sensitive plasmid pCas (0.2 cm-gap cuvettes (Bio-Rad), in a Gene pulser Xcell electroporation system (Bio-Rad)) ...
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bioRxiv - Systems Biology 2023Quote: ... the culture was harvested for electroporation with the pooled pTS041 plasmid library (0.1 cm Gene Pulser Cuvette #165-2089 and Micropulser, BioRad). Cells were recovered in SOC medium with kanamycin and 1 µM aTc for Cas9 induction at 30°C for 2 h and streaked out on to LB agar plates with kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... The resulting donor DNA was electroporated in the plasmid-bearing host using Gene Pulser Xcell Electroporation Systems (Bio-Rad) and the successfully edited clones were selected via colony PCR and Sanger sequencing of the motAB locus ...
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bioRxiv - Microbiology 2023Quote: ... A total of 50 μL of cells was mixed with plasmids and then subject to electroporation (Bio-Rad, USA) at 2.5 kV ...
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bioRxiv - Microbiology 2023Quote: ... We added 1.0 mg of various CRISPRi plasmids to the 0.2 ml fusobacterial competent cells in a chilled cuvette (0.1 cm electrode gap, Bio-Rad), allowing it to rest on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA and siRNA oligonucleotides were transfected into U373 cells using electroporation with a GenePulser Xcell (Bio-Rad Laboratories). Electroporation was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... using 20 mg of pGP-pcDNA3.1 Puro-CAG-Voltron2-ST plasmid DNA and 1.6 μm gold microcarriers (Bio-Rad). Slices were transfected using a 3D-printed version of the modified barrel design (O’brien et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... For immunohistochemistry (IHC) experiments following primary antibodies were used: IgG anti-human CD3 antibody (1:200, clone CD3-12, Bio-Rad) and anti-PCNA antibody (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and growth factors in culture supernatants of control or R1881 treated LNCaP cells was determined using the Bioplex Pro Human Cytokine 17-plex assay system (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Microbiology 2019Quote: ... Isotypes of HuMAbs in culture supernatant were determined by western blot using anti–human IgG conjugated with horseradish peroxidase (HRP) (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-SARS CoV-2 Spike Neutralizing antibody (SAD-S35, ACRO, USA) or anti-Human IgG Kappa (STAR 127, Bio-Rad, USA) antibody ...
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bioRxiv - Biochemistry 2021Quote: The details of the protein purification protocol for human plasma sample are described in the Aurum serum mini kit leaflet and a detailed was followed(BioRad, USA). The sample was prepared for 2-D gel electrophoresis following passive rehydration for 16-24 h ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Molecular Biology 2019Quote: The SPR studies to determine conformation binding of the human chimeric 1A8 and 7C8 mAbs were carried out with the ProteOn XPR36 protein interaction array system (Bio-Rad) based on SPR technology ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Genomics 2020Quote: ... The concentrations of cytokines in plasma were measured using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit immunoassay run on the Bio-Plex 200 (BioRad), with reference to an 8 point standard curve.
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bioRxiv - Immunology 2021Quote: ... Serum was also collected for cytokine analyses which were performed by the University of Pennsylvania’s Human Immunology Core using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: Plasma from each patient sample was analyzed using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit (Bio-Rad). Cytokine expression was measured using the Luminex xMAP® (Luminex Corporation ...
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bioRxiv - Immunology 2021Quote: ... Sera were collected for cytokines analyses which were performed by the Macau University of Science and Technology using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: We used conjugated magnetic beads (6.5 μm) (Bio-Plex Pro Human Chemokine IL-8 / CXCL8 Set #171BK31MR2, BioRad, Hercules, CA, USA) for the binding assay ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Immunology 2024Quote: ... from all experimental groups were evaluated in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Serum cytokines were measured using the Luminex xMAP technology on the Bio-Plex 200 platform (Bio-plex Manager 5.0) with the Bio-Plex Pro™ Human Cytokine Screening Test Kit (48-Plex, Bio-Rad) and the Bio-Plex Pro™ Human Inflammation Panel-1 Kit (37-Plex ...
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bioRxiv - Microbiology 2024Quote: ... IL-28A/IFN-λ2 and IL-29/IFN-λ1) using a Bio-Plex ProTM Human Inflammation Panel 1 Express assay (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pML104-GAL1 and repair DNA were co-transformed into yeast by electroporation under Gene Pulser Xcell (Bio-Rad) conditions ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Molecular Biology 2020Quote: ... ½ of a confluent T75 flask of WT F1 2-1 MS2129 female ESCs were electroporated with 40μg of PciI linearized PCR2.1-Puro XistΔE targeting plasmid (800v, 0.2ms, 4mm cuvette, Biorad X-Cell electroporation module) and plated at varying dilutions on 10cm plates of confluent irradiated DR4 feeders ...
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bioRxiv - Molecular Biology 2020Quote: ... were electroporated with 40μg of Not1 linearized paa2Δ1.7 plasmid DNA (800v, 0.2ms, 4mm cuvette using a Biorad X-Cell electroporation machine) and plated on confluent irradiated DR4 feeders ...
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bioRxiv - Microbiology 2020Quote: ... at 5% parasitemia was resuspended in 300 μL CytoMix containing 75 μg plasmid DNA in a 0.2-cm electroporation cuvette (Bio-Rad). Electroporation was performed at 0.31 kV and 950 μF with maximal capacitance using a Gene Pulser II system (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μL of electrocompetent cells were transformed with 1 ng of the lesion-containing plasmid mixed with 1 ng of pVP146 (internal control for transformation efficiency) by electroporation using a GenePulser Xcell (BioRad) at 2.5 kV ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cell/plasmid mixture was transferred to an electroporation cuvette with 1mm gap and transformed using a Gene Pulser Xcell electroporator (BioRad) with 1.8 kV ...
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bioRxiv - Biochemistry 2019Quote: 40 µl of electrocompetent cells were transformed with 1 ng of the lesion-containing or lesion-free control plasmids together with 1 ng of pVP146 (transformation control) by electroporation (GenePulser Xcell(tm) from BioRad, 2.5kV – 25µF – 200Ω) ...
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bioRxiv - Developmental Biology 2019Quote: ... ~250,000 trypsinised hESCs were resuspended in 600 μl PBS+Ca2++Mg2+ containing 50 μg gRNA plasmid and 50 μg homologous repair donor plasmid and electroporated in a 0.4-cm cuvette using Gene Pulser Xcell System (Bio-Rad) with a single 20 ms square-wave pulse (250 V) ...
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bioRxiv - Synthetic Biology 2020Quote: ... putida cells with selected plasmids using a MicroPulser electroporator and Gene Pulser Cuvettes with 0.2 cm gap (Bio-Rad, USA). Preparation of P ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant plasmids of SCD and SREBP1 genes were transfected into the CEH by using the Gene Pulser XcellTM Electroporation system (Biorad). After transfection ...
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bioRxiv - Plant Biology 2023Quote: ... 1×108 cells were plated on an ESAW agar plate and five μg of plasmid DNA coated on W-microparticles (M17, Biorad) was delivered into the cells using the Biorad Biolistic Particle Delivery System (1550 psi ...
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bioRxiv - Cell Biology 2023Quote: ... Episome plasmids were transformed into E. coli (TransforMax EPI300) harboring the pTA_Mob51 mobility plasmid (gift from R. Lale) via electroporation (Bio-Rad). Transformed cells were spread onto LB agar plates containing both gentamycin (10 μg mL-1 ...
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bioRxiv - Bioengineering 2024Quote: ... The working plasmids were added to 80 µL competent cells in a pre-cooled 0.1-cm MicroPulser Electroporation Cuvette (Bio-Rad) and the mixture was treated in MicroPulser Electroporator (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... amoebiformis CCMP2058 cells were transformed with 10 μg of the plasmid using a Gene Pulser Xcell electroporation system (Bio-Rad), as described previously (Fukuda et al ...
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bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
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bioRxiv - Immunology 2022Quote: The collected fractions were then pooled by the expected oligomeric state for both human milk and serum and again IgAs were captured on beads with a 1% blocking buffer background (Bio-Rad, Netherlands) and addition of the 7D8-IgA1 and 5D5v2-IgA1 at 0.2 μg/mL ...