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Citations for Bio-Rad :
101 - 150 of 314 citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked by using PBS with 5% human AB serum (Bio-Rad, Germany) and 5% mouse serum (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... and human 18S genes using the iQ™ SYBER® Green Supermix (Bio-Rad, #1708880) with the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... For cytokine quantification Bio-Plex Pro Human Cytokine Screening Panel 48-plex (BioRad, USA; 12007283), Bio-Plex Pro Human Cytokine ICAM-1 (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... and the Bio-Plex Pro™ Human Inflammation Panel-1 Kit (37-Plex, Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... All plasmids were transformed into BJ3505 wild-type and elo3Δ strains via electroporation (BIORAD) and plated onto appropriate auxotrophic selection plates.
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bioRxiv - Neuroscience 2020Quote: ... cultures were transfected with plasmids using a biolistic method (Helios Gene Gun, Bio-Rad). To obtain sparse astrocyte labelling we used 1 μm gold particles (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... The plasmids were coated on gold microparticles (1 μm diameter, Bio-Rad, California, USA) and bombarded on about one week old sorghum seedlings according to the protocol of (Jose-Estanyol ...
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bioRxiv - Biophysics 2023Quote: ... plasmid DNA were transfected using 70 ul of Transfectin Lipid Reagent (BioRad, California, USA). 4 or 5 T175 plates were prepared for each plasmid DNA ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products or plasmids linearized by NotI were transfected into cells by electroporation (Biorad). Transfected cells were selected by the addition of 30 μg/ml G418 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products or plasmids linearized by NotI were transfected into cells by electroporation (Biorad). Transfected cells were selected by the addition of 30 μg/ml G418 (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... coated with 10 μg of plasmid DNA using 1,100 psi rupture discs (Bio-Rad). Spectinomycin-resistant lines were selected on RMOP regeneration medium (Svab et al ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with mouse anti-human secondary antibody conjugated to HRP (1721050, Bio-Rad Laboratories). HRP binding was determined based on reaction with TMB substrate (34028 ...
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bioRxiv - Immunology 2020Quote: Supernatants were thawed and immediately analyzed with Bio-plex human cytokine assays (Bio-Rad Laboratories, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into K562s using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Immunology 2024Quote: ... 17 human cytokines were analysed simultaneously using the kit and run on the Luminex analyser (BIORAD), standard curves for each cytokine created and per cytokine concentrations calculated using the BioRad software.
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were transformed with the ligated plasmids under chilled conditions (Bio-Rad Laboratories, Inc., Hercules, CA). Colony-forming units of the libraries were checked with limiting dilution to maintain >107 per microlitre ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... All strains were transformed with the plasmid pSK265::DsRed by electroporation (Gene Pulser, Bio-Rad) and were grown at 37 °C on blood agar (43049 ...
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bioRxiv - Cancer Biology 2019Quote: ... mixed with appropriate plasmids in a 0.4-cm gap cuvette (BIO-RAD, Hercules, CA, USA) and transfection was performed by electroporation using a Gene Pulser II electroporator (BIO-RAD ...
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bioRxiv - Immunology 2021Quote: ... Cells and plasmid DNA in solution were transferred into 0.2 cm generic electroporation cuvettes (Biorad Gene Pulser ...
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bioRxiv - Genetics 2023Quote: ... Plasmid-based ddPCR reactions (20 µL) contained 10 µL of ddPCR Supermix for Probes (Biorad), 1 µL of each 5 µM probe ...
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bioRxiv - Plant Biology 2023Quote: ... Transform the constructed plasmid into NHCC seedlings through particle gun bombardment (Bio-Rad, PDS1000/He). The empty pTY plasmid was used as the control ...
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bioRxiv - Immunology 2021Quote: ... specific for the various human chemokine receptor genes and IQ™ SYBR green reagent (# 170882, BIO-RAD) on CFX96 real-time PCR detection system (BIO-RAD) ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... an assay detecting the human ribonuclease P/MRP subunit p30 RPP30 gene (Bio-Rad Assay ID dHsaCP2500350) was used as reference (probe ...
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bioRxiv - Cell Biology 2022Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... For the quantification of metabolic hormones Bio-Plex Pro Human Diabetes 10-plex Assay (BioRad, USA; 171A7001M) was used ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated at 370C for a further 24 h with human oxLDL (#5685-3557, Bio-Rad) at a concentration of 0 µg/ml and 25µg/ml ...
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bioRxiv - Cell Biology 2019Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... IL-18 and IL-6 concentrations were measured using the Bio-Plex Pro Human Cytokine Assay (BioRad).
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bioRxiv - Immunology 2022Quote: ... Cytokines and chemokines were measured using the Bio-Plex Pro™ Human Assay (Bio-Rad, Hercules, USA): cytokine screening panel plus ICAM-1 and VCAM-1 (12007283 ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into CHO cells using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Immunology 2024Quote: ... Approximately 600 RUs of anti-human IgG (Cytiva) were immobilized on a GLC Sensor Chip (Bio-Rad) using amine-coupling ...
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bioRxiv - Pathology 2024Quote: ... and cytokines were detected using the Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, #M5000031YV), including IL-1β ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:4 and analyzed using Bio-Plex ProTM Human Chemokine Panel 40-plex Assay (Bio-rad). Acquisitions and analyses were performed on a Bio-Plex 200 system with Manager 6.1 Software (Bio-rad) ...
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bioRxiv - Bioengineering 2020Quote: ... Electroporation with the respective plasmid was performed using a Bio-Rad (Bio-Rad Laboratories, Hercules, CA) MicroPulser preprogrammed EC2 setting (0.2 cm cuvettes with 100 µL cells ...
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bioRxiv - Biochemistry 2020Quote: ... cells were electroporated with 20 μg of plasmid in 0.4 cm-gap electroporation cuvettes (Bio-Rad). Cells were electroporated at 225 V ...
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bioRxiv - Physiology 2019Quote: ... The plasmids were evaluated on a 1% agarose gel and displayed on transilluminator (BioRad city, country). The amplification products were finally sequenced in both directions (T7 and SP6).
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bioRxiv - Plant Biology 2019Quote: ... we precipitated 1μg plasmid DNA on 302.5 μg of 1μm gold particles (Biorad, Hercules, CA, USA)by adding the same volume of 1M CaCl2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... electroporating 1—10 ng plasmid with the EC1 setting of a Micropulser (Bio-Rad Laboratories, Hercules) and recovering for 1 hour at 37 °C in SOC media before plating on selective LB-agar.
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bioRxiv - Microbiology 2020Quote: ... The cell suspension containing plasmid DNA was electroporated using a Gene Pulser electroporator (Bio-Rad, USA) with the following parameters ...
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bioRxiv - Genomics 2022Quote: The assembled plasmid was transformed using Gene Pulser Xcell Electroporation Systems by electroporation (BIO-RAD 1652661) into 50 μl of ElectroMax DH10B electrocompetent cells (Invitrogen 18290015 ...
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bioRxiv - Microbiology 2022Quote: ... the samples (cells with plasmid) were added to a 2mm-gap electroporation cuvette (Bio-Rad, USA). The sample was pulsed at 140V and 1,000 μF with an exponential decay on a Bio-Rad GenePulserXcell module ...
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bioRxiv - Biochemistry 2022Quote: ... were transformed with the GFPxm163-pET-28a(+)-TEV plasmid by electroporation (MicroPulser Electroporator, Bio-Rad Laboratories), plated onto a LB (lysogeny broth ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were transformed into onion (Allium cepa) epidermal cells using a particle delivery system (BIO-RAD) as previously described (Yang et al. ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... After sequence confirmation 50 ml cultures were grown in Luria Broth and plasmids extracted (BioRad 7326120).
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bioRxiv - Microbiology 2020Quote: ... Analyses were performed with the Extended Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Biorad, Munich, Germany) as described previously 72 and using a 2-laser reader allowing simultaneous quantification of cytokines and chemokines ...