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Citations for Bio-Rad :
401 - 450 of 7095 citations for DNA Damage 8 OHdG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension is then embedded in 1% low-melt agarose plugs and treated using the CHEF Mammalian Genomic DNA Plug Kit (Biorad), according to the manufacturer’s protocol38,39 ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was synthesized from 1 µg DNA-free RNA using the iSCRIPT cDNA synthesis kit (BioRad, Hercules, CA, U.S.A). Real-time PCR master mixes were prepared using iQ sybr Green Mix (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Complementary DNA (cDNA) from extracted RNA was obtained using the iScript™ cDNA Synthesis Kit (Bio-rad, CAT No.:1708890) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Complementary DNA (cDNA) was made by converting at least 100 ng RNA with the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR (qPCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The agarose gel plugs were prepared and processed with the CHEF Mammalian Genomic DNA Plug Kit (BioRad, Cat. No. #1703591). Total RNAs used to construct RNA-seq libraries were extracted from various tissues of a female juvenile zebra shark (total length ...
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bioRxiv - Physiology 2023Quote: ... was reverse transcribed and converted to complementary DNA (cDNA) using the iScript cDNA synthesis kit (1708891, Bio-Rad, Hercules, CA). Each cDNA sample was amplified with messenger RNA (mRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA (1μg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... IL-23 was measured using either a mouse IL-23 ELISA (R&D) or Luminex based method from BioRad. IL-12p40 ...
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bioRxiv - Immunology 2020Quote: ... followed by measuring the absorbance of individual wells at 450 nm using an ELISA reader (Bio-rad mod.550). Serum samples from unmanipualted mice were used for negative controls.
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bioRxiv - Plant Biology 2023Quote: ... and CCD1 (5’-GGGAAGAGGGTGATGAAGTTGT-3’ and 5’-TGATATCCATTCACCTTGTCCAAA-3’) and 5 µl of SsoAdvanced Universal SYBR Green Supermix (172-5270; BioRad, Hercules, CA, USA). All samples were analyzed in triplicate using the CFX connect Real-Time PCR Detection System (1855201 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR plate was subsequently heat-sealed with pierceable foil using a PX1™ PCR plate sealer (Bio-Rad) and then amplified in a LifeEco thermal cycler (Bioer ...
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bioRxiv - Biophysics 2021Quote: ... The PCR plate was subsequently heat-sealed with pierceable foil using a PX1 PCR plate sealer (Bio-Rad, USA) and then amplified in a Veriti thermal cycler (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Droplets were transferred to a 96-well PCR plate and heat-sealed using PX1 PCR Plate Sealer (Bio-Rad). PCR amplification was performed with the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... the OD600 of the 96-well plate was recorded by a plate reader (iMarkTM microplate absorbance reader, Bio-Rad). The minimum inhibitory concentration (MIC ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 µL of SybrGreen (BioRad) and 2.5 µL of primer-mix (800 nM ...
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bioRxiv - Microbiology 2019Quote: ... 5 µl HF buffer (BioRad), 5 µl combinational enhancer solution (2.7 M betaine ...
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bioRxiv - Neuroscience 2023Quote: Four fragments of the tln-1 genomic DNA (gDNA) (pBG-1088) were amplified from N2 genomic DNA using iProof High-Fidelity DNA polymerase (BioRad) or Q5® High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... 25 ng of cDNA template was mixed with the primers to final concentrations of 200 nM and mixed with 5 μl of iTaqTM Universal SYBR® Green Supermix kit (Bio-Rad). The reactions were done in triplicates with the following conditions ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Samples were separated on 8–16 % Mini-PROTEAN® TGX™ Precast gels (Bio-Rad) and transferred onto nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... in Tris-glycine-SDS running buffer or 3-8% Criterion XT Tris-acetate gels (BioRad) in Tris-acetate-SDS running buffer ...
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bioRxiv - Immunology 2019Quote: ... lysates were resolved using precast Mini-PROTEAN TGX gels 8-16% gradient gels (Bio-Rad) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 8–12% SDS-PAGE and transferred to a nitrocellulose membrane (BioRad). The membrane was blocked in 5% milk ...
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bioRxiv - Immunology 2020Quote: ... Samples were separated on 8–16 % Mini-PROTEAN® TGX™ Precast gels (Bio-Rad) and transferred onto nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked 10 min with 8% (w/v) non-fat dry milk (Bio-Rad) in Tris-buffered saline (TBS)-T and stained with primary antibodies (listed in material section ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated on 8–16 % Mini-PROTEAN® TGX™ Precast gels (Bio-Rad) and transferred onto nitrocellulose membrane ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cultures were dispensed in 100 μL aliquots into 8-well PCR strips (Bio-Rad) and incubated for 24 h in a thermal gradient using a DNA Engine Tetrad 2 Peltier Thermal Cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The products were analyzed by 8% acrylamide/bis-acrylamide (19:1 ratio, Bio-Rad, 1610145) gel containing 1x TBE and 7M Urea ...
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bioRxiv - Cell Biology 2023Quote: ... 20μg protein was isolated on 8-10% SDS-PAGE and transferred to nitrocellulose membrane (BioRAD). Nonspecific binding was blocked by incubation of membranes with 10% (W/V ...
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bioRxiv - Synthetic Biology 2023Quote: ... with 0.2 ml Flat PCR Tube 8-Cap Strips (Bio-Rad™, optical, ultraclear, #TCS0803). Optimum amplification conditions were determined for each by amplifying six concentrations of a 5× dilution series of MG1655 pJK14-Tn4rev pZA31-mCherry-tnpB using two-step amplification with a thermal gradient of 55–75°C ...
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bioRxiv - Microbiology 2023Quote: ... 40 µg of proteins were loaded onto 3–8% precast polyacrylamide gel (Bio-Rad, 3450129). After migration ...
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bioRxiv - Immunology 2024Quote: ... TNF-α) were analyzed using Bio-Plex Pro Mouse Cytokine 8-plex Assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... anti-CD8 (2 µg/rat dissolved in saline solution, clone OX-8, Bio-Rad #MCA48G) or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA was reverse transcribed to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Biorad, Hercules, California) in a 20 μL reaction volume ...
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bioRxiv - Plant Biology 2019Quote: Agarose plug embedded nuclei were Proteinase K treated for two days followed by RNAse treatment (Biorad CHEF Genomic DNA Plug Kit). DNA was recovered from agarose plugs according to IrysPrep™ Plug Lysis Long DNA Isolation guidelines (BioNano Genomics) ...
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bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was quantified on a Bio-Rad T100 96-Well PCR Gradient Thermal Cycler with the SYBR Green kit (Bio-Rad, SsoAdvanced™ Universal SYBR® Green Supermix) ...
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bioRxiv - Microbiology 2022Quote: ... about 25 ng of DNA-free total RNA for each lncRNA was reverse-transcribed using iTaq cDNA Synthesis Kit (Bio-Rad) and then analyzed with ABI 7500 Fast Real-time PCR System (Applied Biosystem ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Genetics 2020Quote: ... Equal amounts of DNA-free total RNA were converted to cDNA using the iScript™ cDNA synthesis kit (Bio-Rad #1708891). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: Cell pellets from 2 mL overnight cultures were used to create gel plugs using the CHEF Bacterial Genomic DNA Plug Kit (Bio-rad). DNA was digested with I-SceI ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then performed quantitative polymerase chain reaction (qPCR) using 2 ng of complementary DNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Cell Biology 2023Quote: ... diluted to 1 ng/μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription to create complementary DNA (cDNA) with the iScript cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions in a total volume of 20 μl ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA Synthesis Kit protocol (Bio-Rad Laboratories Inc.; Hercules, USA), and the concentration was determined using a Qubit flex fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: The bacterial and fungal loads in extracted DNA samples were quantified by RT-qPCR using the iTaq Universal SYBR Green One-Step kit (Bio-Rad). Each reaction was prepared in a final volume of 20 µL containing 5 µL of template DNA ...
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bioRxiv - Physiology 2023Quote: ... Custom primers were designed to meet several criteria using NCBI Primer Blast followed by running of the complementary DNA (cDNA) synthesis product made using RT-PCR (iScript kit, Cat no. 1708890, Biorad, USA) on an electrophoresis gel ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with QIAquick® PCR purification kit and 1-10 ng of immunoprecipitated or input DNA were amplified with iQ™ SYBR Green Supermix (BioRad) using a Real-Time PCR machine (BioRad IQ™ 5) ...