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Citations for Bio-Rad :
101 - 150 of 5423 citations for Cow NADH ubiquinone oxidoreductase chain 5 MT ND5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: The iTaq-Universal SYBR Green Supermix was used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) by following the manufacturer’s instructions (Bio-Rad, USA). The first strand cDNA samples were diluted 3 times prior to qRT-PCR ...
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bioRxiv - Physiology 2023Quote: ... was used to run the quantitative polymerase chain reaction (qPCR) using the CFX 380 Real-Time system C1000 TM Thermal Cycler (Bio-Rad), with the following parameters ...
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bioRxiv - Cell Biology 2023Quote: 5 to 10 ng of cDNA were used to perform quantitative polymerase chain reaction using 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). All RT-qPCR reactions were performed in a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... quantitative real-time polymerase chain reaction (qRT-PCR) using gene-specific primers and universal SYBR Green Supermix (#1725274, Bio-Rad Laboratories) was performed ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance of the suspension was measured at 570nm using ELISA plate reader (BioRad, USA). Cellular cytotoxicity was determined in duplicates and each experiment was repeated thrice independently.
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bioRxiv - Immunology 2021Quote: ... The absorbance at 450 nm was measured by an ELISA plate reader (Bio-Rad). The endpoint serum dilution was calculated with curve fit analysis of optical density (OD ...
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bioRxiv - Immunology 2023Quote: ... The plates were developed with TMB ELISA substrate solution (Bio-Rad Laboratories, Hercules, CA) and stopped using 1 N sulfuric acid ...
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bioRxiv - Neuroscience 2021Quote: ... Transcript levels were assessed via real-time quantitative polymerase chain reaction (qPCR) amplifications using an iQ SYBR Green Supermix (BioRad, Hercules, CA) with primers listed in Table 4-1 on a CFX96 system (BioRad) ...
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bioRxiv - Cancer Biology 2019Quote: ... One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR) using iScript supermix (Biorad, Hercules, CA). The sequence of PCR primers used are ...
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bioRxiv - Neuroscience 2020Quote: ... levels were quantified by real time polymerase chain reaction (RT-PCR) using SYBR® Green DNA intercalating dye and master mix (Bio-Rad) and the ABI 7900HT PCR machine ...
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bioRxiv - Biochemistry 2020Quote: ... Real time polymerase chain reaction (RT-PCR) was performed with the CFX96 or CFX384 Touch(tm) Real-Time detection system (Bio-Rad Laboratories), using a SensiMix(tm ...
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bioRxiv - Neuroscience 2022Quote: ... Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) was performed using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) using 3 ng of cDNA per reaction and Sensifast SYBR No-ROX mix (Bioline Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNA aliquots were analyzed in simultaneous real-time quantitative polymerase chain reactions using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Canada) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were performed using a C1000 TouchTM Thermal Cycler (Bio-Rad, Hercules, CA, USA). The reaction system included 5 μL SYBR® Premix Ex TaqTM (Takara ...
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bioRxiv - Immunology 2019Quote: ... One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR) using iScript supermix (Biorad, Hercules, CA). The sequence of PCR primers used are ...
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bioRxiv - Microbiology 2021Quote: ... Fifty (50) μl polymerase chain reaction (PCR) mixtures were carried out in a T100™ Thermal Cycler (Bio-Rad Laboratories, Hercules, CA) using 100 ng of total DNA template along with AmpliTaq® DNA Polymerase reagents (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: Droplet digital polymerase chain reaction (ddPCR) was performed using whole-slide FFPE extracted DNA on a QX200 Droplet Digital PCR System (BioRad, Hercules USA) following previously described protocols [20 ...
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bioRxiv - Bioengineering 2023Quote: The gene abundance of total bacteria and nitrifiers were determined by the quantitative polymerase chain reaction (qPCR) using the CFX real-time PCR detection system (Bio-Rad, USA). The primer sets chosen for AOB ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA obtained was analyzed by quantitative polymerase chain reaction (qPCR) using the CFX96 real-time PCR detection system (Bio-Rad Laboratories). The PCRs were performed in 20 µl reaction volumes that included 50 mM Tris–HCl (pH 8.6) ...
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bioRxiv - Genomics 2020Quote: ... 5 μl of total RNA was reverse transcribed using the iScript Advanced cDNA Synthesis Kit (BioRad, USA) in a 10 µL volume ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM EGTA) and protein concentrations were determined by DC protein assay kit II (Bio-Rad #5000112). Proteins were separated on house-made SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham #10600003) ...
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bioRxiv - Molecular Biology 2019Quote: ... Measurement of serum cytokines was performed using multiplex ELISA (human 27-plex #m500kcaf0y, Bio-Rad).
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bioRxiv - Immunology 2020Quote: Mouse CXLC10 protein was quantified using ELISA (eBioscience; BMS6018MST) and Bio-Plex (Bio-Rad; #12002244). Human recombinant IFN-α2 was from Biolegend (592704 ...
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bioRxiv - Physiology 2019Quote: ... Real time polymerase chain reaction (RT-PCR) was performed with the CFX96 or CFX384 Touch™ Real-Time detection system (Bio-Rad Laboratories), using a SensiMix™ (BioLine ...
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bioRxiv - Physiology 2019Quote: ... Transcript levels were analyzed by real-time polymerase chain reaction (RT-PCR) (fluorescence detection of SYBR green) in an iCycler iQ apparatus (Bio-Rad, Hercules, CA), with normalization against 18S as an endogenous control ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse Transcription – Quantitative Polymerase Chain Reaction (RT-qPCR) reactions were performed using SsoAdvanced™ universal SYBR® green (Bio-Rad, Hercules, CA, USA) and run on the CFX Connect (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time polymerase chain reaction for all targets was performed on the CFX-96 Real Time System using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA). Products were confirmed by gel electrophoresis ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: For the separation of myosin heavy chain isoforms a large format SDS-PAGE setup with integrated cooling core was used (Protean II xi, Bio-Rad Laboratories, Inc.). A 5% stacking gel ...
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bioRxiv - Molecular Biology 2023Quote: Relative genetic expression was determined using Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) (CFX96™ Real-Time System, Bio-Rad, CA, USA) using commercially available TaqMan primers (Tables 1-3 ...
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bioRxiv - Immunology 2023Quote: ... Viral RNA genome was detected and quantified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on a BioRad real-time CFX96 detection system (BioRad, Hercules, United States). The target sequence for amplification was the viral RNA-dependent RNA polymerase [26].
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Quantitative real time polymerase chain reaction (qPCR) was performed using the iQ™ SYBR® Green Supermix (170888, Bio-Rad, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... We optimized the recommended polymerase chain reactions (PCR) cycling conditions and performed a touchdown PCR in a T100 thermocycler (Bio-Rad, Hercules, CA). The total reaction volume was 50 µl which contained 2 µl of genomic DNA at 50 ± 25 ng/µl ...
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bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Oligo-dT first strand cDNA were synthesized from 5 μg total RNA by iScriptAdv cDNA kit (Biorad, # 1725038) following the manufacturers recommendations ...
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bioRxiv - Immunology 2022Quote: ... Optical density (OD) was immediately read at 450 nm using an ELISA plate reader (Bio-Rad).
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bioRxiv - Biophysics 2024Quote: ... 5% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... were removed from the eluted samples post-purification and quantified using quantitative Real Time polymerase chain reaction using the Bio-Rad CFX 96™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and Promega Plexor®HY Quantitation System (Promega Corporation ...
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bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
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bioRxiv - Microbiology 2021Quote: ... Optical densities (OD) were measured at 450 nm using an ELISA plate reader (Spectrophotometer Bio-Rad Xmark). Cut-off values were assessed as the average OD plus two standard deviations obtained from serum (n = 100 ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Physiology 2022Quote: ... real-time quantitative polymerase chain reaction (RT-qPCR) was executed with 13 ng cDNA using SSO advanced SYBR Green supermix (Bio-Rad Laboratories, Hercules, CA, USA) with the following settings ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana (5’-CAGGCGGTGGAAACTACCAAG-3’ and 5’-TACAGCACTGCACGGGTCGAT-3’) and R129_E (5’-CGAGCTAATCTCCAAAAGCCATC-3’ and 5’-TGACCCTACCGTGGTTAGCTG-3’) with iQ SYBR Green Supermix (BIO-RAD), as described previously (Garrido-Oter et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein concentrations of the supernatants were determined using DC™ Protein Assay Kit (Bio-Rad #5 000 112). Equal concentrations of protein (30 μg ...