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Citations for Bio-Rad :
601 - 650 of 2215 citations for Caspase 12 CASP12 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Proteins were separated by SDS-PAGE in 10 or 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (0.45μm BioRad or 0.2μm Whatman) in a wet blotting apparatus ...
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bioRxiv - Physiology 2020Quote: Electrophoresis was performed using a BioRad Mini-PROTEAN cell with 4-12% or 4-15% precast TGX gels (BioRad, Hercules, CA) in TGS running buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad). Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat) ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Pathology 2020Quote: ... 5 microliters of urine were run on 4-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie Blue Stain Solution (Bio-Rad) to detect proteinuria ...
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bioRxiv - Molecular Biology 2021Quote: ... were boiled in SDS-PAGE sample buffer and loaded onto each lane of a polyacrylamide gel (Criterion XT Bis-Tris Gel 12%, Bio-Rad). Following electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... then 20 μg of protein was resolved on a 4-12% Tris gel and transferred to a PVDF membrane using the Trans-Blot Turbo system (Bio-Rad) according to standard protocols ...
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bioRxiv - Neuroscience 2020Quote: ... following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX, Biorad, Munich, Germany) and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Developmental Biology 2019Quote: ... were used to standardize amount of total protein loaded on a gel 12% polyacrylamide gel was then transferred to polyvenyidene fluoride (PVDF membrane, Bio-Rad). 5% nonfat dry milk in PBST (Phosphate buffered saline with Tween 20 ...
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bioRxiv - Genomics 2019Quote: The volume for approximately the same number of cells for each sample across the mitotic release time course was loaded in each lane of a 4-12% bis-tris protein gel (Biorad 3450125) and separated in 1X MES running buffer (Biorad 1610789) ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was incubated at 37°C for 30 minutes then left to cool for 10 minutes before adding 6 μL of 0.2% 2-mercaptoethanol in SDS-page loading dye.38 Samples were then heated to 94°C for 2 minutes and added to the wells of a pre-cast 12% polyacrylamide gel (Bio-Rad). The gel was run for 45 minutes at 120 V in running buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins (20 μg – 40 μg depending on the target protein) were resolved by 10%-12% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (BioRad, USA). The membranes were blocked with 5% non-fat milk for 2 h at RT and subsequently probed with specific primary antibodies overnight at 4°C (Table EV2) ...
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bioRxiv - Neuroscience 2021Quote: ... In a water bath samples were boiled for 2 minutes and 12 ul of each lysate were loaded on two separate gels (Biorad, #4568096), one each required for parallel HTT and pS13 HTT detection ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 µg protein was separated by electrophoresis on 4-12% polyacrylamide gels followed by transfer to nitrocellulose membranes (all gels/reagents from Bio-Rad). Membranes were then blocked (5% milk in TBS-Tween buffer ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated on 8-12% SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). The membrane was incubated overnight with the respective primary antibodies at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... For the single cell sort 1.4 × 107 cells from pig 12 were stained with CD3-RPE (Clone: PPT3, BIO-RAD, UK), CD8α-RPE (Clone ...
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bioRxiv - Immunology 2021Quote: ... Cell lysate proteins were resolved on a 4–12% gradient SDS-PAGE gel and transferred to Trans-Blot nitrocellulose membrane (Bio-Rad). Blots were incubated with polyclonal goat anti-mouse TLR2 Ab (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Evolutionary Biology 2022Quote: Protein samples were analysed by size-based separation through SDS-PAGE using either 12% polyacrylamide gels or Any kD polyacrylamide gels (Bio-Rad) (81) ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... were performed using the FIREPol® Master Mix Ready to Load (Solis biodyne, 04-12-00115) and a T100 thermal cycler (Biorad). Sequences of the primers used are the following ones ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty to 40 µl of the lysate were loaded into a 12 % polyacrylamide gel for SDS-PAGE electrophoretic (BioRad. Mini-PROTEAN) separation of proteins ...
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bioRxiv - Cell Biology 2022Quote: ... separated into an 8%-12% SDS-PAGE gels and transferred to a nitrocellulose Hybond-C membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% milk in PBS-0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Polyacrylamide gels (shear modulus: 16 kPa—final concentrations of 12% acrylamide [1610140, Bio-Rad] and 0.15% bis-acrylamide [1610142, Bio-Rad]) were embedded with 0.04-μm fluorescent microspheres (F8789 ...
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bioRxiv - Cell Biology 2022Quote: ... Polyacrylamide gels (shear modulus: 16 kPa—final concentrations of 12% acrylamide [1610140, Bio-Rad] and 0.15% bis-acrylamide [1610142, Bio-Rad]) were embedded with 0.04-μm fluorescent microspheres (F8789 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with beta-mercaptoethanol were boiled for 5 min and separated on 12% SDS-PAGE gels prior to transfer to methanol-activated PVDF membranes using the Trans-Blot Turbo system (Bio-Rad). Membranes were blocked in Tris-buffered saline with Tween-20 (TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... were separated on 12% (w/v) SDS-PAGE gels and transferred to an Immun-Blot low fluorescence-PVDF membrane (Bio-rad). The membrane was first blocked with Odyssey Blocking Buffer (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein (30 μg) was resolved on a 12% SDS-PAGE and transferred to a 0.45 μm nitrocellulose membrane (BioRad Cat# 1620115). The membranes were probed with anti-MAG ...
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bioRxiv - Immunology 2022Quote: 2 µg of GST or GST-IFITM3 protein was loaded into a 12% acrylamide Criterion XT Bis-Tris Precast Gel (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 15-30 μg of protein lysate was loaded and separated on 12% Tris-glycine SDS-PAGE gels and transferred to nitrocellulose membranes (catalog number 1620167, Bio-Rad) using Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2022Quote: ... Equal amounts of protein extracts were resolved by 10%-12% SDS-PAGE and electrotransferred onto a polyvinylidene membrane (Bio-Rad, USA). After blocking in Tris-buffered saline plus 5% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Developmental Biology 2022Quote: Protein extracts were separated on a 12% SDS gel with a 5% stacking gel and transferred to PVDF membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked using 5% milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS‒PAGE in 12% acrylamide gels and then transferred onto PVDF membranes using a Trans-Blot Turbo Transfer System (Bio-Rad) and PVDF transfer packs (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 15 µg of protein from each sample were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) (12%) and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad) for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: Formalin-fixed and paraffin-embedded 5μm sections were stained with hematoxylin and eosin (HE), DCT (Pep8h), SOX10 (clone EP268, Cell Marque, 383R-15) or CD3 (clone CD3-12, BioRad, MCA1477) antibodies using standard immunohistochemistry methods ...
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bioRxiv - Neuroscience 2023Quote: ... An equal amount of sample was loaded and separated on a 12% bis-tris gel and transferred to PVDF membranes (1620177 Bio-Rad) using a Bio-Rad cassette at 70 volts for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... then 20 μg of protein was resolved on a 4-12% Tris gel and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo system (Bio-Rad) according to standard protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 5-10μg of total proteins were separated on bis-tris polyacrylamide gradient gels (NuPAGE Novex 4-12% Bis-Tris Gel, Life tech, #NW04120BOX) or TGX stain-free gels (BioRad, #4568126) and transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... pulldown samples collected as described above were loaded on a 4-12% gradient Criterion XT Bis-Tris precast gel (Bio-Rad). The gel was fixed in 40% methanol and 10% acetic acid and subsequently stained for 1h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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bioRxiv - Biophysics 2024Quote: ... total proteins and biotinylated proteins were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Microbiology 2023Quote: Dilution series containing equal amounts of protein were prepared and separated on 12% Mini-PROTEAN TGX gels (BioRad, Hercules, CA, USA) by SDS-PAGE and then transferred onto 0.2 µM polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...