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Citations for Bio-Rad :
451 - 500 of 2215 citations for Caspase 12 CASP12 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... proteins from the supernatant were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Microbiology 2019Quote: ... Fractions of 1 ml were recovered from the top to the bottom of each tubes and loaded on a gradient 4-12 % SDS-PAGE Bis-Tris gel (BioRad). For Western Blot analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were eluted in SDS sample buffer and 30 μl of each sample was run on a 12% Bis-Tris 1D SDS-PAGE gel (Biorad) for 1 cm and stained with colloidal Coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... About 80 µg of denatured proteins were loaded for each condition and separated on gradient 4–12% polyacrylamide classic or TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were resolved on 4-12% Criterion XT Bis-Tris or 4-20% Mini-Protean TGX precast gels (BioRAD) and transferred to Immobilon-FL PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated by SDS-PAGE on 12% polyacrylamide gels and transferred to a Hybond ECL membrane (Amersham biosciences) using a semi-dry transfer system (Biorad). Membranes were subsequently blocked by incubation for 1 h at room temperature in 5 % (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µg of total protein per sample was loaded on NuPage 4-12% Bis-Tris Protein gels (Thermo Fischer Scientific) or 4–15% Mini-PROTEAN TGX StainFree Protein gels (Biorad) and run at 200 V as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants aspirated and loaded on to 4-12% NuPage Bis/Tris gels for separation before transfer on to a Nitrocellulose membrane (Biorad) at 4°C at a constant current of 350mA in transfer buffer (25 mM Tris ...
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bioRxiv - Synthetic Biology 2020Quote: ... 25μl of each sample was loaded onto a 12% SDS PAGE gel and then transferred to 0.45 mm nitrocellulose membranes (Bio-rad). Protein loading was assessed through ponceau staining of nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell extracts were prepared using UREA lysis buffer and 30-60μg of total protein was loaded on a 7.5% or 12% denaturing polyacrylamide self-cast gel (Bio-Rad, Switzerland). Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074 ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... and boiled at 95°C for 5 min before loaded onto a 4-12% SDS/PAGE gel and run at 200 V before electrotransferred to a membrane using Transblot-Turbo Transfer system (BioRad). The membrane was then washed 2 × 15 min in TBS with 0.1% Tween20 (TBST) ...
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bioRxiv - Bioengineering 2021Quote: Protein samples were mixed with Laemmli buffer and the proteins were separated by SDS-PAGE using either a 7.5 % or a 12 % polyacrylamide gel and a Tris-glycine running buffer (Bio-Rad). The Precision Plus Protein™ Unstained Standard (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... respectively 45 μL and 10 μL of proteins were loaded for each condition and separated on gradient 4-12% polyacrylamide TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
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bioRxiv - Neuroscience 2021Quote: ... and the volumes corresponding to 35 μg were loaded onto TGX Stain-Free FastCast 12% polyacrylamide gels (Bio-Rad, USA) for electrophoretic separation ...
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bioRxiv - Neuroscience 2021Quote: Samples were resolved by SDS-PAGE using either 10 or 12% acrylamide/bis-acrylamide gels and transferred to 0.22 µm nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 12 μg of total protein were loaded onto a 4-20% mini Protean TGX gel (Bio-Rad, Hercules, CA) and blots were evaluated as described previously
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were separated by electrophoresis in 12 % SDS-polyacrylamide gel and electrophoretically transferred to a polyvinylidene fluoride membrane according to the manufacturer’s instructions (Bio-Rad). Transferred proteins were detected with Ponceau-S ...
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bioRxiv - Neuroscience 2021Quote: ... self-cast 10% or 12% SDS-gels or Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). After electrophoretic separation ...
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bioRxiv - Physiology 2021Quote: ... About 30 μg of protein from the sample extracts were electrophoresed in 12% Mini-PROTEAN TGXTM precast gels (Bio-Rad) using the electrophoresis system mini-PROTEAN Tetra Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were run on 12% polyacrylamide gel and transferred to a nitrocellulose membrane using the Trans-blot SD semi-dry transfer cell (Biorad). To represent the same number of cells in each lane ...
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bioRxiv - Molecular Biology 2020Quote: ... Running buffer was prepared with tris-glycine 1X and SDS 0.1% using a homemade gel (7-12% bis-acrylamide, Bio-Rad), which ran at 100 V for approximately 2:30 h ...
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bioRxiv - Microbiology 2020Quote: ... Samples were run in 3-12% Bis-Tris gradient gels and transferred to PVDF membranes in a wet blot system (BioRad). Membranes were dried ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Physiology 2022Quote: ... and was and then electrophoretically separated at 240V for 22 minutes on a 7-12% TGX gradient gel (BioRad, 4568081). Proteins were then transferred to a PVDF membrane using a BioRad Trans Blot Turbo ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was separated by SDS-PAGE using 12% acrylamide Mini-protean TGX precast gels (Bio-Rad, USA). Gels were stained with Coomassie (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 60 μg of protein was separated on 10% SDS/PAGE (Mini-PROTEAN TGX Gels,12%,10-well, 4561043, BIO-RAD) and transferred onto nitrocellulose membrane (#1620115 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein samples were loaded on 10%-12% polyacrylamide gels and transferred onto 0.45 µm pore-size nitrocellulose membranes (Bio-Rad) using the Turboblot (BioRad) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracted from tobacco or kiwifruit were separated on 12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad) using a highly efficient wet protein transfer system (eBlot L1 ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... Samples were run on a precasted gradient gel (4 – 15% Criterion TGX, 12+2 wells, 45 μl, BioRad, CA, USA) for 45 min at 60 mA ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Molecular Biology 2020Quote: ... and adjusted before isolation of protein aggregates as well as verified by running reduced samples on Criterion XT Precast Gel 4-12% Bis-Tris (BioRad) and staining with Coomassie Brilliant Blue R-250 staining solution (BioRad) ...
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bioRxiv - Physiology 2021Quote: ... Proteins (50 μg) were loaded onto 12–15% SDS-PAGE gels using a Mini-Protean apparatus (Bio-Rad, CA, USA) and transferred onto nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... An equal amount of total protein (30μg) were resolved on precast 4-12% SDS-PAGE gels (Biorad, Hercules, CA, USA), transferred onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: Samples were prepared for standard SDS-PAGE and separated on 12% acrylamide gel (Mini-PROTEAN TGX precast gels, Bio-Rad), then transferred onto 0.2 µm pore size PVDF membranes (Millipore ...
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bioRxiv - Genetics 2022Quote: ... and a 2-μl aliquot was used in a 12-μl PCR reaction with SsoAdvanced Universal Probes Supermix (Bio-Rad) and specific TaqMan primer/probe assays for Gaa (Taqman assay #Mm00484581_m1 ...
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bioRxiv - Cell Biology 2022Quote: ... and equivalent amounts of protein (∼30 μg) were separated by SDS-PAGE (1.0 mm thick 4 to 12% [w/v] gradient polyacrylamide Criterion Bio-Rad) in MOPS buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: ... The soluble protein extracts were separated by SDS-PAGE (4-15% mini-protein TGX precast gel, 12 or 15 well, BioRad) and transferred to a PVDF membrane (Trans-blot Turbo mini-Format 0.2 μM PVDF ...
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bioRxiv - Microbiology 2019Quote: ... and separated on a 12% polyacrylamide gel before being transferred onto Trans-Blot® Turbo™ Midi (PVDF) membranes (Biorad) using the Trans-Blot® Turbo™ Transfer System (Biorad) ...
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bioRxiv - Developmental Biology 2019Quote: ... the samples were run in a 12% SDS-polyacrylamide gel and Western blots were developed using an ECL Western blotting kit (Biorad). Rabbit anti-countin antibodies were used at 1 ...
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bioRxiv - Immunology 2019Quote: ... Equal amount of cell lysates (10 μl) were loaded in 4–12% Mini-PrROTEAN TGX Precast protein Gels (Bio-rad) and transferred to PVDF membrane ...
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bioRxiv - Developmental Biology 2019Quote: Proteins were separated by SDS-PAGE on a 4-12% Novex gradient gel then transferred to nitrocellulose membrane with the Trans-Blot Turbo Transfer System (BioRad). Membranes were blocked in 50% Odyssey Blocking Buffer in 0.3% PBST (1x PBS with 0.3% Tween ...
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bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted directly in sample buffer and samples were separated on a 4-12% Criterion Bis-Tris gel (BioRad). Proteins were transferred to PVDF membrane (Immobilon-FL ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were separated by denaturing electrophoresis in 12% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad) with a Mini-Protean Tetra Vertical Electrophoresis Cell at 100V for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were heated at 95°C for 5 min and separated on a 12% Bis-Tris SDS-PAGE gel (Biorad).