Labshake search
Citations for Bio-Rad :
601 - 650 of 3775 citations for 8 DECYLOXYPYRENE 1 3 6 TRISULFONIC ACID TRISODIUM SALT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2023Quote: ... Blotting membrane was primed for 3 min prior in cold transfer buffer (BIORAD 10026938), according to manufacturer specifications ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... kinase-dead extracts were incubated 3 consecutive times with protein A-beads (Bio-Rad) previously coated with the anti-B55δ antibody (1 hour incubation at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Reactions were loaded onto a 3% agarose gel and visualized on a Chemidoc (BioRad). Bands were densitometry quantified by ImageJ and the percentage of methylated DNA was calculated over the total intensity of all the quantified bands.
-
bioRxiv - Molecular Biology 2023Quote: ... 3 μg of the protein samples were prepared with 4X Laemmli loading buffer (Biorad) and denatured at 95°C for 5 minutes ...
-
bioRxiv - Immunology 2023Quote: ... 3) live cell populations using live-dead exclusion dye (Zombie Aqua, #423101, Bio-Rad); 4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
-
bioRxiv - Immunology 2024Quote: ... Samples were diluted in Laemmli sample loading buffer supplemented with |3-mercaptoethanol (Bio-Rad), heated for 5 min at 95°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated overnight at 4 °C with rabbit anti-chick IL-6 (Bio-Rad Laboratories, Inc., Hercules, CA) diluted 1:20 in incubation buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... Excess dye was removed by two consecutive buffer exchanges through Micro Bio-Spin P-6 desalting columns (Bio-Rad). Protein concentration and labeling efficiency were estimated using a NanoDrop Microvolume Spectrophotometer.
-
bioRxiv - Genomics 2019Quote: Binding reactions were incubated at room temperature for 30 min and loaded onto a 6% polyacrylamide 0.5 × Criterion precast TBE gel (Biorad). After sample electrophoresis and transfer to a nylon membrane ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.14 M NaCl (PBS) followed by removal of the reductant using a Micro Bio-Spin 6 column (Bio-Rad). Columns were washed and pre-equilibrated with 20 mM phosphate buffer pH 7.4 containing 0.1 mM diethylenetriamine-penta-acetic acid (DTPA ...
-
bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
-
bioRxiv - Biochemistry 2020Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 7.5 with a BioSpin 6 column (BioRad).
-
bioRxiv - Microbiology 2022Quote: All proteins were buffer exchanged into 0.2 M ammonium acetate using Bio-Spin P-6 Size Exclu-sion Spin Columns (BioRad) as previously described47 ...
-
bioRxiv - Genetics 2022Quote: ... PMOs were buffer exchanged into 25 mM ammonium bicarbonate buffer pH 7.5 using Biospin 6 (Bio-rad, Hercules, California) columns according to the manufacturer’s protocol and then spinning through an 0.22 µm cellulose acetate centrifugal spin filter ...
-
bioRxiv - Neuroscience 2019Quote: ... Neurons were biolistically transfected after 5-6 days in vitro using a Helios Gene Gun (120 psi; Bio-Rad) with pLenti-hSyn-eNpHR3.0-EYFP (eNpHR3.0 fused to EYFP and driven by the human synapsin I promoter ...
-
bioRxiv - Genetics 2019Quote: ... 10 mM L-serine) to a UapA concentration of 20 μM using Micro Bio-Spin 6 columns (Bio-Rad). UapA was loaded into gold coated capillaries and the protein sprayed into a Synapt G2-Si (Waters ...
-
bioRxiv - Neuroscience 2019Quote: ... and each qPCR reaction was performed with 6 ng cDNA and Bio-Rad IQ SYBR Green Supermix (Bio-Rad CFX384 Touch Real Time PCR Detection System ...
-
bioRxiv - Biochemistry 2019Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 8.0 with a BioSpin 6 column (BioRad).
-
bioRxiv - Evolutionary Biology 2020Quote: ... The Chlorella DNA was extracted by isolating the Chlorella and then using a standard 6% Chelex100 resin (Bio-Rad) extraction method ...
-
bioRxiv - Biochemistry 2019Quote: ... Buffer exchange or removal of excess NTPs and abortive products were performed by gel filtration (BioRad Micro-Spin 6), if necessary.
-
bioRxiv - Microbiology 2021Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories). Protein (final concentration 20 µM ...
-
bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
-
bioRxiv - Immunology 2022Quote: ... Excess free Texas Red or TAMRA were removed with Bio-spin 6 kDa size exclusion columns (Bio-Rad Laboratories) equilibrated with PBS according to manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (Healthcare Life Sciences) were loaded into Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
-
bioRxiv - Genomics 2023Quote: ... Excess dye was removed using Micro Bio-Spin® Columns with Bio-Gel® P-6 (cat:7326221, BioRad) by centrifugation at 1000g ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The reactions were run at 125 V for 2.5 hours on a denaturing (6 M urea) 20% (w/v) polyacrylamide gel in 50 mM NaOAc pH 5.2 running buffer (using the Biorad Mini- PROTEAN tetra gel ...
-
bioRxiv - Microbiology 2023Quote: ... resuspended in 400 µL cold PBS and electroporated with 6 µg of RNA in 0.4-cm electroporation cuvettes (Biorad) with a 950 μF and 260 V pulse using Genepulser system (Biorad) ...
-
bioRxiv - Physiology 2024Quote: ... 6 μg of protein from each sample was loaded into a 4-20% Mini-PROTEAN® TGX gel (BioRad) and proteins were separated by size using electrophoresis ...
-
bioRxiv - Plant Biology 2023Quote: ... Samples containing 6 µg total protein were resolved using pre-cast SDS-PAGE Any KD™ gels (BIO-RAD), transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 µm ...
-
bioRxiv - Microbiology 2022Quote: ... The reaction was stopped with 1N sulfuric acid and absorbance was measured at 450 nm in the iMarkTM Microplate Absorbance Reader (BioRad).
-
bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
-
bioRxiv - Microbiology 2019Quote: Aliquots of extracted nucleic acid from plasma were processed in triplicate (1.5ul per replicate) following the recommended workflow (WX200 ddPCR system, Bio-Rad). Custom PrimeTime (IDT ...
-
bioRxiv - Physiology 2020Quote: ... Samples and standards were incubated with bicinchonic acid for 30 minutes prior to measuring absorbance as 540 nm using an iMark microplate absorbance reader (BioRad).
-
bioRxiv - Microbiology 2022Quote: ... A Bio-Rad Aminex HPX-87H HPLC column for organic acid analysis (300 × 7.8 mm; Bio-Rad Hercules, CA, USA) was equilibrated with 5 mM H2SO4 (Titrisol ...
-
Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
-
bioRxiv - Biochemistry 2022Quote: ... Gels were Coomassie stained and destained with 10% acetic acid prior to imaging on a ChemiDoc MP imaging system (BioRad).
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed on a 4-15% polyacrylamide NuPage Bis-Tris gel (invitrogen) in 1x morpholineethanesulfonic acid (MES) SDS running buffer (invitrogen) or on a 4-20% polyacrylamide Criterion gels (BioRad) in 1x Tris/glycine/SDS buffer (BioRad ...
-
bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Protein separation was done by 1D SDS-PAGE electrophoresis using commercial gels to ensure reproducibility (8–16% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad). A sample of venom of ISm and ISy lines ...
-
bioRxiv - Neuroscience 2020Quote: ... Diabetes-associated markers and cytokine levels in the plasma were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Cat# 171F7001M; Bio-Rad, Carlsbad, California) and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD ...
-
bioRxiv - Cell Biology 2020Quote: ... Total protein (5–20 μg) was resolved onto 8–12% SDS-PAGE and blotted onto 0.2 μm pore size PVDF membranes (1620177, Bio-Rad, Hercules, CA, USA). Primary antibodies were incubated overnight at 4°C after blocking membranes for 1h ...
-
bioRxiv - Microbiology 2021Quote: All melt-MAMA PCRs were performed on a CFX96 Real-Time PCR Detection System using white hard shell 96-well PCR plates or 0.2 mL 8-tube PCR strips (Bio-Rad, Gladesville, NSW, Australia), and PCR results examined using the CFX Maestro v4.1.2433.1219 software (Bio-Rad) ...
-
bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
-
bioRxiv - Immunology 2020Quote: ... 30-50µg of protein per lane alongside a prestained molecular weight protein marker was separated on an 8% SDS PAGE gel prepared from SDS-PAGE kit (Beyotime, China) and electrotransferred to immunoblot PVDF membrane (BIO-RAD, CA, USA) for protein blotting ...
-
bioRxiv - Molecular Biology 2022Quote: ... chromatin samples were dissolved in SDS-containing loading buffer and the electrophoresis was carried out in 8-16% mini-protean TGX Bio-Rad gels (Cat. # 4561105, Bio-Rad, Hercules, CA) gradient acrylamide gels as described (Laemmli ...
-
bioRxiv - Immunology 2022Quote: ... Equal amount of total protein (25 μg/well) was resolved by electrophoresis on 8% Tris-Acrylamide gels and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad Laboratories, CA, US). Membranes were blocked for 1 h at room temperature in 5% skim milk diluted in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST) ...
-
bioRxiv - Microbiology 2023Quote: Serum samples collected from mice were tested in duplicate in a multiplex immunoassay using magnetic beads (Mouse Th1-Th2 8-plex Bio-Rad, Hercules, CA) using the Luminex MAGPIX® system) ...
-
bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).