Labshake search
Citations for Bio-Rad :
201 - 250 of 396 citations for 7 bromoquinoline 8 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2021Quote: ... Real-time PCR was performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo-Fisher) using the iTaq Universal SYBR green Supermix (Bio-Rad). The following primer sequences were used ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were transferred electrophoretically at a constant voltage of 25 V for 7 min using a Trans-Blot Turbo Transfer System (Bio-Rad). After transfer ...
-
bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
-
bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
-
bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 30 °C for 7 days and imaged daily using a gel documentation system (Gel Doc™ XR+, BIORAD). When needed ...
-
bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... RNA was transferred to a nylon membrane (Amersham Hybond-XL) at 23 V for 7 minutes using a Trans-Blot Turbo Transfer System (Bio-Rad). Following transfer ...
-
bioRxiv - Cell Biology 2023Quote: Protein samples were resolved on 7-15% gradient polyacrylamide (SDS-PAGE) gels and transferred to nitrocellulose membranes via Trans Blot Turbo (Bio-Rad). Membranes were then blocked with 5% milk in Tris-buffered saline and 0.05% Tween 20 (TBST ...
-
bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
-
bioRxiv - Cancer Biology 2021Quote: After running through 8% SDS-polyacrylamide (SDS-PAGE; made in house) by gel electrophoresis (BioRad, Hercules, CA, USA), the proteins were transferred ...
-
bioRxiv - Microbiology 2019Quote: ... Total cell lysates were separated on 8-12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with TBST with 5% (w/v ...
-
bioRxiv - Genomics 2021Quote: ... were resolved on 8-12% Tris-glycine SDS-polyacrylamide gels and transferred to nitrocellulose blotting membranes (Bio-Rad), following standard procedures ...
-
bioRxiv - Microbiology 2021Quote: ... Cell lysates and virion lysates were resolved on 8 to 16% Mini-Protean TGX precast gels (Bio-Rad), transferred onto nitrocellulose membranes (GE Healthcare ...
-
bioRxiv - Plant Biology 2021Quote: ... were loaded onto an 8-channel DG8TM cartridge for droplet generation in the QX200 Droplet Generator (Bio-Rad). Droplets were transferred into a 96-well plate (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... SDS-PAGE was performed using 8-16% gradient gel and transferred to nitrocellulose paper using the Transblot (BioRad). Western blots were carried out using iBind with 1 ug/ml primary antibodies and 1 ug/ml secondary antibodies following iBind protocol (Invitrogen/ThermoFisher).
-
bioRxiv - Genomics 2020Quote: ... quantifying 8 mRNAs and 10 circRNAs of interest with the SsoAdvanced™ Universal SYBR Green Supermix (BioRad, USA). All reactions were performed in 384-well plates on the LightCycler480 instrument (Roche ...
-
bioRxiv - Biochemistry 2021Quote: ... samples were buffer exchanged into 150 mM of ammonium acetate pH 8 using Bio-Spin P6 columns (BioRad) and the final protein concentrations were adjust to 1-5 µM ...
-
bioRxiv - Microbiology 2022Quote: ... Cell lysates and virion lysates were resolved on 8 to 16% Mini- Protean TGX precast gels (Bio-Rad), transferred onto nitrocellulose membranes (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: ... 8 ng of granuloma protein was loaded per lane into Any kD Mini-PROTEAN TGX gels (Bio-Rad), separated by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... serum insulin was performed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Bio-Rad, Hercules, CA) as per the manufacturer’s protocol.
-
bioRxiv - Physiology 2022Quote: ... The 8% equivalent of each fraction by volume was run on a 4-15% TGX gel (Bio-Rad) for Western blotting using affinity-purified polyclonal rabbit anti-mouse Prdm13 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 8 μl from each sample were loaded onto Mini Protean TGX Pre-cast 4-15% gels (Bio-Rad) and migration was carried out for approximately 45 min at 150V ...
-
bioRxiv - Molecular Biology 2023Quote: 4–8 μl of samples and 3 μl of Precision Plus Protein Dual Color Standard (1610374, Bio-Rad) were loaded into 4–12% Bis-Tris Bolt gels (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... separated on 6% acrylamide 8 M urea gels and transferred to Zeta Probe GT membranes (Bio-Rad laboratories) by electroblotting ...
-
bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
-
bioRxiv - Genomics 2023Quote: ... the ligation products were amplified by 8 cycles of PCR on an S1000 thermocycler (Bio-Rad, Hercules, CA) using Q5 High-Fidelity PCR master mix with HF buffer (NEB Ipswich ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant from two reactions were collected and separated by Criterion XT Tris Acetate 3-8% (Bio-rad) SDS-PAGE and visualized by silver staining.
-
bioRxiv - Biophysics 2024Quote: ... 20 µL of the supernatant was taken and added to 8 µL 4X Laemmli sample buffer (Bio-Rad) and 12 µL of 8M urea ...
-
bioRxiv - Biophysics 2020Quote: ... 50 µl of a 10− 6 M biotinylated and 10−7 M dye-coupled oligonucleotide solution were co-incubated to anneal the oligonucleotides at 80 °C using a Thermocycler (BioRad, C1000 ThermoCycler), followed by sample cooling to 4 °C with -1 °C/min ...
-
bioRxiv - Immunology 2021Quote: ... The cells were centrifuged at 300G for 7 minutes at 4°C and the cell pellet was resuspended then counted with the BioRad cell counter (Bio-Rad, TC20).
-
bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
-
bioRxiv - Neuroscience 2021Quote: ... Lcn2 and C3 were quantified by quantitative real-time PCR (qRT-PCR) using the QuantStudio 7 Pro System thermo-cycler (ThermoFischer Scientific) and SYBR Green Master Mix (Bio-Rad Laboratories). Data are shown as fold change relative to control samples using the ΔΔCq method with Rpl4 as an internal control gene ...
-
bioRxiv - Cancer Biology 2022Quote: ... Quantitative real-time PCR was performed with the Applied Biosystems ViiA 7 system using iTaq Universal SYBR Green Supermix (Bio-rad #1725121). Human actin was used as a house keeping control ...
-
bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
-
bioRxiv - Genomics 2019Quote: ... Resulting single-cell GEMs were collected at the completion of the run (~7 min) and linear amplification was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; 1851197): 72°C for 5 min ...
-
bioRxiv - Neuroscience 2023Quote: ... and image captured by continuous exposure for 7 minutes with a Bio-Rad ChemiDoc MP Imaging System (12003154, Bio-Rad, CA, USA). The protein expression was quantified by determining individual band density using ImageJ (FIJI ...
-
bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
-
bioRxiv - Cell Biology 2020Quote: 10-40 µg of total protein lysate were separated by SDS-PAGE on 8% or 10% polyacrylamide minigels (BioRad) and transferred onto a nitrocellulose membrane (GE healthcare ...
-
bioRxiv - Molecular Biology 2021Quote: Proteins were separated by SDS-PAGE using a Mini-PROTEAN TGX Gel (4-15% or 8-16%, Bio-Rad Laboratories ...
-
bioRxiv - Physiology 2019Quote: ... 8 µg of total protein was separated by electrophoresis on a precast 4-15% TGX gradient gel (Bio-Rad) and transferred to a 0.45 µm nitrocellulose membrane ...
-
bioRxiv - Microbiology 2019Quote: Samples were loaded onto SERVAGel™ TG PRiME 8-16% precast gels and transferred on PVDF membranes (Bio-RAD). Proteins were detected with primary antibodies anti-St1SctP (InvJ ...
-
bioRxiv - Microbiology 2021Quote: ... The proteins were resolved by SDS-PAGE using 8–16% Mini-PROTEAN TGX Stain-Free protein gels (Bio-Rad) and transferred onto a PVDF membrane using a semi-dry apparatus (Trans-Blot Turbo Transfer System ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were subjected to SDS-PAGE by resolving on a 3-8% CriterionTM XT Tris-Acetate gel (Bio-Rad) for immunoblotting.
-
bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
-
bioRxiv - Biochemistry 2022Quote: All analytical gels were performed in 20% polyacrylamide gels (acrylamide:bisacrylamide 19:1) using 8×12 cm plates in a mini-PROTEAN system (BioRad). Acrylamide solution was prepared in 1X TBE buffer (89 mM Tris ...
-
bioRxiv - Genomics 2019Quote: ... we first assembled the 8-strip tubes into 96-well plates for increased throughput (Bio-Rad, RC9601 and MSA5001). For FACS-collected cells we proceeded directly with the obtained 96-well or 384-well plates ...
-
bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).