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Citations for Bio-Rad :
151 - 200 of 396 citations for 7 bromoquinoline 8 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... glucose was added to make 8% final concentration and loaded onto commercial Criterion Precast Gel (Biorad) 5% TBE ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA template (8 μL) was added to 10 μL of ddPCR Supermix for Probes (Bio-Rad) and 2 μL of the primer/ probe mixture ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μL of lysate was resolved on 8% SDS-PAGE and transferred to Nitrocellulose membrane (BioRad). Membranes were blocked with 5% milk in Tris-buffered saline with 1% Tween 20 (TBST ...
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bioRxiv - Physiology 2021Quote: ... Relative protein abundance (N=6-8) were quantified using the Image Lab software (version 6.0.1; BioRad) and normalized by the protein content in each lane.
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bioRxiv - Immunology 2022Quote: ... Proteins were subjected to 8–12% SDS-PAGE and transferred to PVDF membranes (Bio-Rad Laboratories). The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... the nuclear fraction proteins were resolved on gradient 8-16% gels (mini-PROTEAN TGX, Bio-Rad). Resolved proteins were transferred to nitrocellulose membranes (Trans-blot Turbo ...
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bioRxiv - Microbiology 2023Quote: ... 8 μL of sample or buffer or protein standards solution (Precision Plus Protein Kaleidoscope, Bio-rad) were loaded per well of pre-cast 12 % polyacrylamide gel (10-well Mini-PROTEAN TGX ...
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bioRxiv - Biochemistry 2023Quote: ... was prepared for electrophoresis and loaded onto an 8-16% Mini-Protean TGX gels (Bio-rad). Following this ...
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bioRxiv - Microbiology 2023Quote: ... Elution fractions were analyzed on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and fractions containing the gp120 glycoprotein were concentrated followed by buffer exchange to PBS using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were run on 8% SDS-PAGE gel for 1 hour at 175V (Tetra cell, BioRad). Following migration ...
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bioRxiv - Cell Biology 2021Quote: ... DNA samples (3 μl) and master mixes (7 μl) were pipetted into a 96-well PCR plate (Bio-Rad #MLL9651) and PCR amplification performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Genetics 2021Quote: ... Proteins (7 μl of extract) were separated on Laemmli SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, 1620112). Membranes were stained with Ponceau-S for visualization ...
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bioRxiv - Immunology 2021Quote: ... A 2.7 μL aliquot from each sample was mixed with 2.5 μL of SsoFast EvaGreen Supermix with Low Rox (Bio-Rad) and 0.25 μL of Fluidigm’s DNA Binding Dye Sample Loading Reagent in a separate plate and centrifuged to mix solutions ...
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were separated by electrophoresis in 7% SDS-polyacrylamide gels and electrophoretically transferred to a polyvinylidene fluoride membrane according to the manufacturer’s protocol (Bio-Rad). Transferred proteins were detected with Ponceau-S ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes using either a Trans-Blot® Turbo™ Transfer System (7 minutes, 25V, BioRad) or an iBlot™ 2 Dry Blotting System (P0 method ...
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bioRxiv - Molecular Biology 2020Quote: ... Running buffer was prepared with tris-glycine 1X and SDS 0.1% using a homemade gel (7-12% bis-acrylamide, Bio-Rad), which ran at 100 V for approximately 2:30 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were in-gel rehydrated for 16 hrs and isoelectrically focused on 7 cm pH 3-10 IPG strips to 10,000 Vh on a Protean® IEF Cell (BioRad). After focusing ...
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bioRxiv - Biochemistry 2022Quote: ... Insoluble material was pelleted by repeated ultracentrifugation and the supernatant recovered and loaded onto an hydroxyapatite column (7 g dry-weight resin, Biorad 130-0420, hydrated and packed in a disposable chromatography column, Biorad 732-1010 ...
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bioRxiv - Biochemistry 2022Quote: ... Insoluble material was pelleted by repeated ultracentrifugation and the supernatant recovered and loaded onto an hydroxyapatite column (7 g dry-weight resin, Biorad 130-0420 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on native acrylamide gels containing 0.5X TBE buffer and 7% acrylamide (made from a stock of 40% acrylamide with a ratio of 29:1 acrylamide:bisacrylamide; Bio-Rad). The running buffer for the gels was 1XTBE ...
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bioRxiv - Physiology 2022Quote: ... and was and then electrophoretically separated at 240V for 22 minutes on a 7-12% TGX gradient gel (BioRad, 4568081). Proteins were then transferred to a PVDF membrane using a BioRad Trans Blot Turbo ...
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bioRxiv - Biochemistry 2019Quote: ... autophosphorylation reactions were separated on SDS-PAGE gels and transferred to 0.2 µm nitrocellulose membranes at 25 V for 7 mins using Trans-Blot Turbo Transfer system (Bio-Rad). Membranes were blocked with BSA and incubated with primary mouse anti-phosphotyrosine antibody (PY20 ...
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bioRxiv - Bioengineering 2019Quote: Native PAGE gels were prepared as follows: a fresh solution comprising of polyacrylamide (19:1) at final concentrations from 7 to 13% (Biorad), 0.5x TBE buffer (45 mM Tris-borate ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were clarified by centrifugation at 6,000 rpm for 7 min and protein concentration was determined using a DC protein assay kit (Bio-Rad). Samples containing equal amounts of protein were brought to a total volume of 1 ml with appropriate buffer.
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...
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bioRxiv - Cell Biology 2023Quote: ... phosphorylated in vitro by PLK-1 or CyclinB-Cdk1 as described (7) were separated on stain Free SDS-PAGE 10% gel (Biorad). The gel was imaged and then transferred to a PVDF membrane 0.45 µm during 1h30 at 90V ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µL bound and 7 µl input sample was separated on 4-15% TGX SDS-PAGE gel (BioRad #456-1086) and transferred to PVDF membrane (Immobilon-FL ...
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bioRxiv - Plant Biology 2023Quote: Shoot apices of reporter lines were dissected and fixed in 4% paraformaldehyde for 1 hour and embedded in 7% ultra low range agarose gel (BioRad). Fifty µm sections were prepared using a vibratome DTK-1000 (Dosaka ...
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bioRxiv - Neuroscience 2023Quote: ... High integrity RNA (RIN > 7) was used to synthesize cDNA with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). PCR primers for human B-ACTIN ...
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bioRxiv - Cell Biology 2023Quote: ... and were then transferred to polyvinylidene difluoride (PVDF) membrane using a semi-dry method with a Trans-Blot Turbo system for 7 minutes at 25V (BioRad). The membranes were probed with the rabbit polyclonal antibody against an amino-terminal region of β1 [21] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM EDTA) using the Mini-PROTEAN Tetra Vertical Electrophoresis cell (gel size 10×8 cm, BioRad). Each run was preceded by a 30 min pre-run at 300 V to warm the gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated on an 8-16% gradient gel (Pierce) and transferred to a PVDF membrane (Biorad). The membrane was blocked in 5% milk for 1 h and incubated overnight with primary antibody (diluted 1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... histones were separated at 100V and 250mA on 8-16% Criterion TGX precast gels (Bio-Rad Laboratories). After fixation with a buffer containing 7% acetic acid and 10% methanol ...
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bioRxiv - Microbiology 2020Quote: ... and loaded into the wells of a polyacrylic gradient gel (8-16%) using a Kaleidoscope kit (BioRad) as a molecular weight marker ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... and densitometric analyses of bands were conducted using the Image Lab v5.1 built 8 (Bio-Rad, USA).
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bioRxiv - Cell Biology 2021Quote: ... sample aliquots were used for protein separation on 8-16% TGX-Criterion gels (Bio-Rad, Hercules, CA). After electrophoresis ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal protein loads of 8ug were resolved on precast 8% to 16% Bis-Tris gels (Bio-Rad) under reducing conditions ...
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Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... 10–15 μL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V in 1x Tris-Acetate buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 10–15 µL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V to separate Rad53 isoforms ...
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bioRxiv - Microbiology 2023Quote: ... 8% SDS) and subjected to immunoblot analysis using the Stain-Free Imaging Technology (BioRad, Hercules, CA, USA). After SDS-Page ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were separated by SDS-PAGE in an 8-16% gradient Tris/Glycine gel (Bio-Rad #5671105) then transferred onto a nitrocellulose membrane by using the Trans-Blot Turbo RTA transfer kit (Bio-Rad #1704271) ...
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bioRxiv - Plant Biology 2023Quote: ... and 8 µL of the supernatant were loaded onto a precast mini-PROTEIN TGX Polyacrylamide Gel (BIORAD). After running for around 100 min at 160 V ...
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bioRxiv - Microbiology 2023Quote: ... were electrophoretic separated on Mini-PROTEAN TGX Precast Gels (8-16% polyacrylamide, Bio-Rad Laboratories, California, USA) at 70V for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8) and fractions analyzed via 12 % SDS-PAGE followed by Bio-Safe Coomassie Stain (Biorad,1610787). The peak fractions were combined and dialyzed in dialysis buffer (50 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was analysed by SDS-PAGE using 3-8% Criterion XT Tris-Acetate gels (Bio-Rad) and XT Tricine running buffer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... 30 second at 72°C and a final extension of 7 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). Five μl of PCR product was visualized on a non-denaturing 1% agarose gel with a 100bp ladder ...
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bioRxiv - Neuroscience 2021Quote: ... 40 min) and were subsequently transferred to nitrocellulose membranes for semi-dry immunoblotting (25 V, 1,3 A, 7 min, Bio-Rad®). Membranes were dried 5 min at 37°C and then incubated with 5% non-fat dried milk in Tris-buffered salineTween-20® (TBST containing 28 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: Two micrograms of each RNA sample was loaded on 10% polyacrylamide gels containing 7 M urea or loaded onto 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 85 V ...