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Citations for Bio-Rad :
351 - 400 of 1329 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed using EBC buffer (Bio-Rad Cat. # C-1410) supplemented with protease EDTA-free (Cat # ...
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bioRxiv - Neuroscience 2023Quote: ... and 72°C 15 s using a PCR machine (T100, Biorad). PCR primer pairs used were 5’-TCCCAGCCTGAAAGCCACAG-3’ and 5’-ATGCTGCTGGGATTCTCTCCC-3’ for Pet1 ...
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bioRxiv - Genetics 2023Quote: ... Membranes were incubated overnight at 4°C with STAR5B Streptavidin:HRP (BioRad) at a 1:15,000 dilution in 1x PBST ...
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bioRxiv - Neuroscience 2024Quote: ... the iScript™C gDNA Clear cDNA Synthesis Kit (BIO-RAD) was employed ...
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bioRxiv - Cell Biology 2020Quote: 10-40 µg of total protein lysate were separated by SDS-PAGE on 8% or 10% polyacrylamide minigels (BioRad) and transferred onto a nitrocellulose membrane (GE healthcare ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were separated by SDS-PAGE using a Mini-PROTEAN TGX Gel (4-15% or 8-16%, Bio-Rad Laboratories ...
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bioRxiv - Physiology 2019Quote: ... 8 µg of total protein was separated by electrophoresis on a precast 4-15% TGX gradient gel (Bio-Rad) and transferred to a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Microbiology 2019Quote: Samples were loaded onto SERVAGel™ TG PRiME 8-16% precast gels and transferred on PVDF membranes (Bio-RAD). Proteins were detected with primary antibodies anti-St1SctP (InvJ ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were resolved by SDS-PAGE using 8–16% Mini-PROTEAN TGX Stain-Free protein gels (Bio-Rad) and transferred onto a PVDF membrane using a semi-dry apparatus (Trans-Blot Turbo Transfer System ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were subjected to SDS-PAGE by resolving on a 3-8% CriterionTM XT Tris-Acetate gel (Bio-Rad) for immunoblotting.
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
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bioRxiv - Biochemistry 2022Quote: All analytical gels were performed in 20% polyacrylamide gels (acrylamide:bisacrylamide 19:1) using 8×12 cm plates in a mini-PROTEAN system (BioRad). Acrylamide solution was prepared in 1X TBE buffer (89 mM Tris ...
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bioRxiv - Genomics 2019Quote: ... we first assembled the 8-strip tubes into 96-well plates for increased throughput (Bio-Rad, RC9601 and MSA5001). For FACS-collected cells we proceeded directly with the obtained 96-well or 384-well plates ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Cancer Biology 2022Quote: ... The reaction mixture was separated on an 8% native polyacrylamide gel and visualized using a ChemiDoc Imaging system (BioRad) with SYBR Green detection filter.
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bioRxiv - Cancer Biology 2023Quote: ... and resolved by electrophoresis on a 4-20% or 8-16% polyacrylamide gradient gel (Mini-Protean TGX, Bio-Rad). Proteins were transferred to nitrocellulose or PVDF membranes (Immobilon-P membrane ...
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bioRxiv - Microbiology 2024Quote: ... 2 % fetal bovine serum and 8 % chelated fetal bovine serum using Chelex® 100 Resin (BioRad, Ref. 142-1253). To differentiate HaCaT cells ...
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bioRxiv - Developmental Biology 2021Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
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bioRxiv - Immunology 2021Quote: ... Basically 300 ug of IVIG protein were subjected to isoelectric focusing (IPG) across pH gradients of 4-7 (Biorad, LifeSciences, Hercules, Ca, USA) or pH 6-11 (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to nitrocellulose membrane by semi-dry transfer using the Trans-Blot Turbo Transfer System (BioRad, 1.5 A for 7 min). Membranes were blocked in 5% milk in 1x PBS with 0.1% Tween 20 (PBST ...
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bioRxiv - Biophysics 2023Quote: Proteins samples were kept on ice and buffer exchanged into 200 mM ammonium acetate buffer at pH 7 using Micro Bio-spin columns with either a 6 or 30 kDa cutoff (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM before analysis ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (0.2 µm pore size) using the Trans-Blot® Turbo™ semi-dry transfer system (7 min mixed MW MIDI program) (Bio-Rad). After blocking in a 2.5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... whole cell lysates were separated on 8% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (BioRad). The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: E18.5 cortical protein samples were separated on 8% SDS-PAGE gels for 1hr at 120V and transferred overnight at 20V on to PVDF membranes (BioRad). Blots were probed with rabbit anti-tubulin ß3 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The dried pellets enriched for histones were dissolved in sample buffer (8 M urea, 5% β-mercaptoethanol and 10 mM Tris-HCl (pH 7.0)) and added sample loading buffer (Biorad XT sample buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS lysates from beads after co-IP were resolved on 8% SDS polyacrylamide gels and transferred to nitrocellulose membranes (BioRad). Membranes were blocked with 5% low-fat milk in TBS buffer supplemented with 0.1% Tween 20 (blocking buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA template (up to 8 μL) and nuclease-free water were loaded into a disposable droplet generator cartridge (Bio-Rad). After adding 70 μL of droplet generation oil (Bio-Rad) ...
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Transmembrane Signaling Regulates the Remodeling of the Actin Cytoskeleton: Roles of PKC and AdducinbioRxiv - Cell Biology 2021Quote: ... RT/PCR products were analyzed by 8% polyacrylamide gel and relative band intensity was quantified using Image Lab (Bio-Rad). Most of the primer sequences used were selected from the FlyPrimerBank (Hu et al. ...
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bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was done in triplicate in a total reaction volume of 10-μl containing 0.5-μl of 1:8 diluted cDNA (validated dilution with dynamic range of amplification) and 5nM primer mix using the CFX384 QPCR machine (Biorad). The list of primers used are listed in Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein were separated via 8% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Bio-Rad, Cat no. #1620177) membrane using transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Samples taken from individual fractions were analysed on denaturing SDS polyacrylamide gels (8 %) stained with Coomassie Brilliant Blue (Bio-Rad). Fractions containing pure (>90 % ...
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bioRxiv - Microbiology 2020Quote: ... brucei were separated by SDS-PAGE (8% or 10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) (48) ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed and then precipitated in 0.1 M glycine pH 3.5 and neutralized with 0.5 M Tris?Cl and 1.5 M NaCl pH 7.4 before SDS-PAGE electrophoresis (8%, at 100 V for 90 min) followed by transfer to a PVDF membrane (BioRad). Membranes were washed in Tris-buffered saline (TBS ...
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bioRxiv - Bioengineering 2020Quote: ... GEMs were transferred to PCR 8-tube strips and GEM-reverse transcription was performed in a C1000 Touch Thermal Cycler (BioRad): 53 °C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were separated on an 8% polyacrylamide gel stained with SYBR Gold (Thermo Fischer Scientific) and revealed using a ChemiDoc MP Imaging System (Biorad). Band intensity was quantified using ImageJ package (https://imagej.nih.gov/ij/) ...
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bioRxiv - Developmental Biology 2020Quote: ... Western blots were performed either with Criterion ™ XT Tris-Acetate Precast Gels 3–8 % (3450130, Bio-Rad, Hercules, CA), XT Tricine running buffer (161–0790 ...
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bioRxiv - Microbiology 2020Quote: ... Maltose-1-phosphate was separated from orthophosphate by anion exchange chromatography using Dowex 1 × 8 100-200 mesh (Bio-Rad, 28 x 1.6 cm ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Samples were run on 8-12% SDS-PAGE gel and transferred to a nitrocellulose membrane using a Trans-Blot Turbo Transfer System (BioRad). Membrane was blocked in 5% milk in TBS + 0.1% Tween blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... 4-8 µL of samples and 3 µL of Precision Plus Protein Dual Color Standard (cat. no. 1610374, Bio-Rad) were run on 4-12% Bis-Tris Bolt gels (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... protein lysates were loaded onto 8% SDS-PAGE gel and transferred to a PVDF membrane using a semi-dry transfer system (Biorad) for 10 min at 2.5 amperes ...
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bioRxiv - Cell Biology 2022Quote: ... 10-30 μL of the supernatants (based on protein abundances) were run on an 8% SDS-PAGE gel and transferred to a nitro-cellulose membrane (Cat. No. 1620112, BioRad) using a semi-dry transfer method (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... equal amounts of protein (2.5 or 10 µg) were separated by SDS-PAGE on 4-12% Bis-Tris or 3-8% Tris acetate gels (Criterion XT, BioRad) and transferred to nitrocellulose using TransBlot Turbo (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... All steps were done manually using 8-strip PCR tubes and PCR reactions were carried out on a 96-plate thermal cycler (Biorad). Libraries were pooled and sequenced on a Nextseq550 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... were resolved on SDS 8-15% polyacrylamide gels and transferred onto nitrocellulose membranes using the Trans-Blot turbo transfer system (BioRad). Membranes were blotted with the primary antibodies against ADMA (Epicypher ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate and dilutions were denatured in 1x Laemmli buffer at 95C for 30 seconds and resolved by SDS-PAGE on a 8-20% gradient gel (BioRad). Following trimming of the gel ...