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Citations for Bio-Rad :
201 - 250 of 1329 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated at a rate of 0.5 °C per minute from 25 °C to 95 °C and fluorescence signals were recorded with the CFX Connect Real-Time PCR Detection System (Bio-Rad). The melting temperatures were analyzed using the derivative plots of the melting curve.
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bioRxiv - Microbiology 2020Quote: ... was added to each well and samples were heated from 25 to 95 °C in 1 °C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation/emission filters of 492 and 516 nm were used to monitor the fluorescence increase resulting from binding of the Sypro Orange to exposed hydrophobic regions of the unfolding MsSepFcore ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were run for 94°C for 2 min followed by 94°C for 15 s and 60°C for 1 min in a BioRad CFX384 Real Time System (Biorad, USA) qPCR machine ...
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bioRxiv - Microbiology 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative −d(RFU)/dT was determined to be the Tm.
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bioRxiv - Cancer Biology 2022Quote: ... then 40 cycles of 15 seconds at 95°C and 60 seconds at 60°C using CFX96 Touch Real-Time PCR Detection System (Bio-rad). A standard curve was derived from the serial dilutions of DNA template following concentrations ...
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bioRxiv - Biochemistry 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative ...
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Microbiology 2021Quote: ... 60°C for 30 sec and 72°C for 30 sec on a Bio-Rad CFX96 qPCR cycler (Bio-Rad, Germany).
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bioRxiv - Microbiology 2022Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (Bio-Rad). Results were analyzed by the Comparative CT Method (ΔΔCt Method) ...
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bioRxiv - Biochemistry 2022Quote: ... was measured over temperatures ranging from 10 °C to 95 °C using a CFX Connect Real-Time PCR Detection System (Bio-Rad). Melting temperature (Tm ...
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and the mixture was heated from 25 to 95°C in 1°C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation and emission filters of 492 and 516 nm respectively ...
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bioRxiv - Neuroscience 2023Quote: ... with a melt-curve from 65 to 95 °C (in 0.5 °C increments per 5 s) using CFX384 Touch Real-Time PCR Detection System (Bio-Rad, 1845384). Quantification of mRNA was determined using the comparative cycles to threshold CT (ΔΔCT ...
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bioRxiv - Immunology 2023Quote: ... extension at 72°C for 30sec and final extension at 72°C for 5minutes) were conducted in Thermocycler (Bio-Rad, USA). Positive control samples were taken from Molecular Parasitology Laboratory ...
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bioRxiv - Physiology 2023Quote: ... Lysates were then incubated for 5 min at 37 °C and 5 min at 75 °C in a thermocycler (T100, Bio-Rad), immediately followed by storage at -80 °C until further processing.
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Developmental Biology 2021Quote: ... was performed in 7 min at 1.3 A and 25 V using a Trans-Blot TurboTM transfer system (BioRad). Recognition and revelation of the his-tagged protein was performed as described for the Dot Blot ...
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bioRxiv - Neuroscience 2019Quote: ... “any kD” (to quantify GDNF protein) or 7% (to quantify CBP) precast polyacrylamide gel (Bio-rad, Hercules, Cal, USA) and transferred to a polyvinylidene difluoride membrane (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... LarA extracted from a 7 cm prep well Mini-PROTEAN® TGX Stain-Free™ gel (#4568091, Bio-Rad) was used for affinity purification of the final antibody.
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bioRxiv - Biochemistry 2023Quote: ... The gels were then transferred to a water-activated nitrocellulose membrane at 1.3A to 25V for 7 minutes (BioRad TurboBlotter ...
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bioRxiv - Microbiology 2023Quote: ... for 7 min at 2.5A and 12V using a Trans-Blot® Turbo™ Transfer System (Bio-Rad #1704150EDU). Membranes were saturated in PBS (137 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on 15% SDS-PAGE and were transferred to PVDF membrane under 2.5A/25V condition for 7 min using a semidry transfer system (Trans-Blot Turbo, BioRad). Transferred membranes were first blocked by TBS-Tween20 (0.1% ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 2% w/v CA and treated with (7% w/v) Chelex100 resin (Bio-Rad, Mississauga, ON, Canada) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... was introduced by electroporation (7 square wave pulses of 30 V, 3ms, 0,1s pause) using a GenePulser Xcell electroporator (Biorad) and a 1 mm cuvette (Biorad) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were then run on either 4-15% (a) or 8-16% (b) precast gels (Biorad), transferred onto 0.1μm nitrocellulose membranes and used for immunoblot detection ...
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bioRxiv - Molecular Biology 2019Quote: ... Either regular (Figure 4 and 5) or stain-free (Figure 8 and 9, Bio-Rad #1610182) 10 % acrylamide gels were used ...
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bioRxiv - Microbiology 2020Quote: ... loaded onto a lab-made 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad, 1610148), run at 200V for 1 hour ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... and subsequently quantified using densitometric analysis using the Image Lab v5.1 built 8 (Bio-Rad, USA). A negative control of BSA (0.75 μM ...
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bioRxiv - Biophysics 2019Quote: ... glucose was added to make 8% final concentration and loaded onto commercial Criterion Precast Gel (Biorad) 5% TBE ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA template (8 μL) was added to 10 μL of ddPCR Supermix for Probes (Bio-Rad) and 2 μL of the primer/ probe mixture ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μL of lysate was resolved on 8% SDS-PAGE and transferred to Nitrocellulose membrane (BioRad). Membranes were blocked with 5% milk in Tris-buffered saline with 1% Tween 20 (TBST ...
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bioRxiv - Physiology 2021Quote: ... Relative protein abundance (N=6-8) were quantified using the Image Lab software (version 6.0.1; BioRad) and normalized by the protein content in each lane.
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bioRxiv - Immunology 2022Quote: ... Proteins were subjected to 8–12% SDS-PAGE and transferred to PVDF membranes (Bio-Rad Laboratories). The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... the nuclear fraction proteins were resolved on gradient 8-16% gels (mini-PROTEAN TGX, Bio-Rad). Resolved proteins were transferred to nitrocellulose membranes (Trans-blot Turbo ...
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bioRxiv - Microbiology 2023Quote: ... 8 μL of sample or buffer or protein standards solution (Precision Plus Protein Kaleidoscope, Bio-rad) were loaded per well of pre-cast 12 % polyacrylamide gel (10-well Mini-PROTEAN TGX ...
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bioRxiv - Biochemistry 2023Quote: ... was prepared for electrophoresis and loaded onto an 8-16% Mini-Protean TGX gels (Bio-rad). Following this ...
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bioRxiv - Microbiology 2023Quote: ... Elution fractions were analyzed on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and fractions containing the gp120 glycoprotein were concentrated followed by buffer exchange to PBS using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were run on 8% SDS-PAGE gel for 1 hour at 175V (Tetra cell, BioRad). Following migration ...
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bioRxiv - Immunology 2020Quote: ... 40 cycles of 15 s 95°C and 1 min 60°C) was carried out using a CFX 96 (BioRad, Hercules, USA) qPCR machine ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 5 s at 95°C and 30 s at 60°C for 40 cycles using an Biorad CFX384 system (Biorad, Hercules, CA). The mRNA levels of each gene were normalized relative to the mean levels of the two housekeeping genes and compared with the data obtained from unstimulated ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Neuroscience 2021Quote: ... was incubated at 65°C for 10 min and at 85°C for 1 min into a T100™ thermocycler (Bio-Rad). The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™) ...
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The mixture was then incubated at 50°C for 30min followed by 85°C for 15sec in a 9600 thermocycler (Bio-rad, Germany).
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-Star Western C Kit (BioRad catalog 170-5070) was used ...
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bioRxiv - Cell Biology 2019Quote: ... 1:500 Rat anti-C-Myc (BioRad, Hercules, CA), Mouse anti-COMMD1 (Novus Biologicals ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated rat anti-mouse IgG1/c (1:2000, Biorad) or rat anti-mouse IgG2a (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... rabbit polyclonal anti-Cytochrome C (AHP2302, BioRad, 1:200), and mouse monoclonal anti-β-actin (A5441-.2ML ...