Labshake search
Citations for Bio-Rad :
501 - 550 of 9008 citations for 7 7a Dihydro 2 2 4 6 6 pentamethyl 1 3 benzodioxol 5 6H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and Bio-Beads SM-2 resin (Bio-Rad) 0.4 g was then added to remove residual detergents from the proteoliposomes ...
-
bioRxiv - Molecular Biology 2023Quote: ... using 2 X SYBR green mix (Bio-rad). All primers used are listed in Table S6.
-
bioRxiv - Immunology 2023Quote: Slides coated with HEp-2 cells (Biorad #26102) were stained with sera diluted 1:25 in 0.2% BSA PBS and stained for 1 hour at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... and 2% bis-acrylamide (Bio-Rad: 161-0140). Soft (0.5kPa ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 2.5% 2-mercaptoethanol (BME, Cat# 1610710, BioRad) and then boiled for 6 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and 2% bis-AA (BioRad, Cat#161–0142) as a cross-linker 81 ...
-
bioRxiv - Cell Biology 2023Quote: ... 25 µL of 2% bis-acrylamide (Bio-Rad), 2.5 µL of 10% ammonium peroxodisulfate (VWR ...
-
bioRxiv - Cell Biology 2023Quote: ... and 2% bis-AA (BioRad, Cat# 161–0142) cross-linker as described elsewhere.(77 ...
-
bioRxiv - Biophysics 2023Quote: ... Semidry SM-2 Bio-Beads (Bio-Rad Laboratories), equilibrated in reconstitution buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... and 2% bis-acrylamide solutions (1610142; Bio-Rad) were mixed with DI H2O at the ratio shown in Table 1 ...
-
bioRxiv - Biophysics 2024Quote: ... biobeads (Bio-Beads SM-2 Adsorbent, Bio-Rad) equilibrated in 20 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and mixed with a one-third volume of 4×Laemmli protein sample buffer (cat. # 1610747, Bio-Rad). Following centrifugation at 20,000×g at 4℃ for 10 min ...
-
bioRxiv - Cell Biology 2022Quote: ... in 4°C for 2-4 hours and the beads were then isolated and eluted through a Bio-Spin® Chromatography Column (Bio-Rad, #7326008) with elution buffer (5 0mM Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The lysate/bead mixtures were incubated at 4°C on a rotating platform for 2 hrs and subsequently poured into Poly-Prep® Chromatography Columns (Bio-Rad #7311550). Unbound fractions were collected through the columns and stored at −20°C ...
-
Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
-
bioRxiv - Microbiology 2020Quote: ... denatured at 95°C for 2 min and amplified for 24 cycles (95°C 15 seconds, 60°C, 4 min) on T100 PCR instrument (Biorad, Hercules, CA, USA). At the end of this process ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
-
bioRxiv - Immunology 2020Quote: ... a total of 6 × 107 cells was incubated in staining buffer with mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:50) at 4°C for 20 min ...
-
bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
-
bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
-
bioRxiv - Microbiology 2022Quote: ... 2×2 mm squares were cut out and placed into double-sided sticky 9×9 mm frame seals (Bio-Rad SLF0201) on a glass slide ...
-
bioRxiv - Molecular Biology 2020Quote: ... CHAPS (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate) were purchased from Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2019Quote: ... RT-qPCR was performed on 2 μl of 1/25 diluted cDNA with a CFX-96 (Biorad) thermocycler ...
-
bioRxiv - Cell Biology 2022Quote: ... Supernatant was mixed with sample loading buffer supplemented with 1% v/v 2-mercaptoethanol (BioRad, Cat #1610710). Sample was not heated to prevent aggregation of the GPCR ...
-
bioRxiv - Zoology 2020Quote: ... Samples were incubated in a 1:1 (w/v) ratio of 2× sample buffer (0.2M DTT, BioRad Laemmli Sample Buffer #1610737) at room temperature for 20 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
-
bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
-
bioRxiv - Cell Biology 2021Quote: ... DNA samples (3 μl) and master mixes (7 μl) were pipetted into a 96-well PCR plate (Bio-Rad #MLL9651) and PCR amplification performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were in-gel rehydrated for 16 hrs and isoelectrically focused on 7 cm pH 3-10 IPG strips to 10,000 Vh on a Protean® IEF Cell (BioRad). After focusing ...
-
bioRxiv - Cell Biology 2022Quote: ... Excess dye was removed by two consecutive buffer exchanges through Micro Bio-Spin P-6 desalting columns (Bio-Rad). Protein concentration and labeling efficiency were estimated using a NanoDrop Microvolume Spectrophotometer.
-
bioRxiv - Genomics 2019Quote: Binding reactions were incubated at room temperature for 30 min and loaded onto a 6% polyacrylamide 0.5 × Criterion precast TBE gel (Biorad). After sample electrophoresis and transfer to a nylon membrane ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.14 M NaCl (PBS) followed by removal of the reductant using a Micro Bio-Spin 6 column (Bio-Rad). Columns were washed and pre-equilibrated with 20 mM phosphate buffer pH 7.4 containing 0.1 mM diethylenetriamine-penta-acetic acid (DTPA ...
-
bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
-
bioRxiv - Biochemistry 2020Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 7.5 with a BioSpin 6 column (BioRad).
-
bioRxiv - Microbiology 2022Quote: All proteins were buffer exchanged into 0.2 M ammonium acetate using Bio-Spin P-6 Size Exclu-sion Spin Columns (BioRad) as previously described47 ...
-
bioRxiv - Genetics 2022Quote: ... PMOs were buffer exchanged into 25 mM ammonium bicarbonate buffer pH 7.5 using Biospin 6 (Bio-rad, Hercules, California) columns according to the manufacturer’s protocol and then spinning through an 0.22 µm cellulose acetate centrifugal spin filter ...
-
bioRxiv - Genetics 2019Quote: ... 10 mM L-serine) to a UapA concentration of 20 μM using Micro Bio-Spin 6 columns (Bio-Rad). UapA was loaded into gold coated capillaries and the protein sprayed into a Synapt G2-Si (Waters ...
-
bioRxiv - Neuroscience 2019Quote: ... and each qPCR reaction was performed with 6 ng cDNA and Bio-Rad IQ SYBR Green Supermix (Bio-Rad CFX384 Touch Real Time PCR Detection System ...
-
bioRxiv - Biochemistry 2019Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 8.0 with a BioSpin 6 column (BioRad).
-
bioRxiv - Evolutionary Biology 2020Quote: ... The Chlorella DNA was extracted by isolating the Chlorella and then using a standard 6% Chelex100 resin (Bio-Rad) extraction method ...
-
bioRxiv - Biochemistry 2019Quote: ... Buffer exchange or removal of excess NTPs and abortive products were performed by gel filtration (BioRad Micro-Spin 6), if necessary.
-
bioRxiv - Microbiology 2021Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories). Protein (final concentration 20 µM ...
-
bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
-
bioRxiv - Immunology 2022Quote: ... Excess free Texas Red or TAMRA were removed with Bio-spin 6 kDa size exclusion columns (Bio-Rad Laboratories) equilibrated with PBS according to manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (Healthcare Life Sciences) were loaded into Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
-
bioRxiv - Genomics 2023Quote: ... Excess dye was removed using Micro Bio-Spin® Columns with Bio-Gel® P-6 (cat:7326221, BioRad) by centrifugation at 1000g ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The reactions were run at 125 V for 2.5 hours on a denaturing (6 M urea) 20% (w/v) polyacrylamide gel in 50 mM NaOAc pH 5.2 running buffer (using the Biorad Mini- PROTEAN tetra gel ...