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Citations for Bio-Rad :
351 - 400 of 9568 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... we ran similar amounts of protein per lane (~10-20 μg, depending on target) on a 4-20% Criterion TGX stain-free gel (Bio-Rad) at 200 V for 50 min with the cassette surrounded by ice ...
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bioRxiv - Biochemistry 2020Quote: ... A 20 µL aliquot was taken at this point and mixed with 20 µL of 2x Laemmli sample buffer (Bio-Rad). 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein electrophoresis was performed by loading 20 μg of each sample onto 4–20% Bio-Rad Mini-Protean TGX Stain-Free Precast Gels (Bio-Rad). Gel imaging was obtained after activation using the Bio-Rad ChemiDoc Touch Imaging System ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of each sample was determined as described before and a total amount of 20 μg protein/sample was resolved on 4-20% gradient gels (Bio-Rad). Proteins were transferred onto nitrocellulose or PVDF membranes futher blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were then washed in PBS and blocked for 1h in 20 mm Tris Buffered Saline with 0.1% Tween-20 (BioRad, catalog #1610781) (TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... The denatured protein samples (20 µg) were then separated on 4-20 % SDS-polyacrylamide gels using a Tris-glycine running buffer (Bio-Rad) and electroblotted onto nitrocellulose membranes (0.2 µm pore size ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl of lysate was separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), and transferred to a PVDF membrane for analysis using α-FLAG antibody (Sigma Aldrich #F3165 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR plate was subsequently heat-sealed with pierceable foil using a PX1™ PCR plate sealer (Bio-Rad) and then amplified in a LifeEco thermal cycler (Bioer ...
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bioRxiv - Biophysics 2021Quote: ... The PCR plate was subsequently heat-sealed with pierceable foil using a PX1 PCR plate sealer (Bio-Rad, USA) and then amplified in a Veriti thermal cycler (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Droplets were transferred to a 96-well PCR plate and heat-sealed using PX1 PCR Plate Sealer (Bio-Rad). PCR amplification was performed with the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... the OD600 of the 96-well plate was recorded by a plate reader (iMarkTM microplate absorbance reader, Bio-Rad). The minimum inhibitory concentration (MIC ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was purified with 1) Aurum Total Mini Kit (Bio-Rad) using the spin column method with DNase 1 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad; Cat#1864022) and same set of primers and probe used for SIV plasma viral load quantification ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of RNA was reverse transcribed (iScript cDNA Synthesis Kit (BioRad)) and the cDNA was purified with QiaQuick PCR Purification Kit (Qiagen) ...
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bioRxiv - Systems Biology 2022Quote: ... total RNA (1 μg) was reverse transcribed using the SuperScript kit (BioRad), and the Real-time PCR was performed in three technical replicates using the Applied Biosystems StepOnePlus Real-Time PCR system and Fast SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... then embedded in 1% low-melt agarose blocks (BioRad Plug Kit #1703591) to preserve DNA integrity ...
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bioRxiv - Molecular Biology 2023Quote: ... One aliquot was used to synthesize single-stranded cDNA starting from 1 µg of total RNA using a commercially available kit (iScript cDNA Synthesis Kit, Biorad).
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bioRxiv - Biophysics 2023Quote: ... Single-stranded cDNA was synthesized from 1 µg of total RNA as template using a commercially available kit (iScript cDNA Synthesis Kit, Biorad). RT-qPCR analysis of nascent mRNA abundance was performed in duplicate using iQ SYBR Green Supermix (Biorad #1708880 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed with 100 μL Laemmli buffer 2x and 20 μL of each sample (including -CL) was loaded in Criterion™ TGX Stain-Free™ Gels (4-20%, BioRad). SDS-PAGE and Western blots were used to reveal the proteins HuR ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract was loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Biorad). After transfer onto nitrocellulose membrane ...
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bioRxiv - Microbiology 2020Quote: ... 20 μL of normalized lysates were loaded into pre-cast 4-20% SDS-PAGE gels (4-20% Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad) alongside size standards (Precision Protein Plus™ ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and 20 µg of protein from each sample was separated by SDS-PAGE on 4–20% precast polyacrylamide gel (Bio-Rad, 4561096). Western blot analyses were performed according to standard protocols using the following antibodies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
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bioRxiv - Immunology 2023Quote: ... a total of 10–20 µg protein was prepared and loaded onto 4 to 20% gradient Mini-PROTEAN TGX Gels (Bio-Rad, 4561093) and electrophoretically separated at 135 V ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by adding 20 μg of protein lysate to 4-20% gradient Mini Protean TGX Stain Free Pre-Cast Gels (Bio-Rad Laboratories). Proteins were then transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... and the absorbance value of each well was measured at a wavelength of 450 nm on microplate ELISA reader (Microplate Manager v5.2.1 software, Bio-Rad Laboratories).
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bioRxiv - Immunology 2021Quote: ... beads were resuspended in a reading buffer 5 min under agitation (800 rpm) on the plate shaker then read directly on a Luminex Bioplex 200 plate reader (Biorad). Average MFI from the baseline samples were used as reference value for the negative control ...
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bioRxiv - Genetics 2022Quote: ... Approximately 43µl of the newly formed droplet solution were transferred into a ddPCR 96-well plate and then sealed at 180°C for 5 seconds in the PX1 PCR Plate Sealer (BioRad). PCR was carried out with the following conditions ...
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bioRxiv - Neuroscience 2022Quote: ... Pipetting steps were performed using the liquid-handling robots Dragonfly or Mosquito HV (SPT Labtech) using 384 well-plates and PCR reactions were carried out on a 384-plate Thermal Cycler (BioRad). Illumina sequencing of the resulting libraries was performed by Novogene (https://en.novogene.com/ ...
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bioRxiv - Cell Biology 2024Quote: ... 96-well PCR plates were sealed with peelable foil heat seals at the PCR plate sealer machine (PX1TM, Bio-Rad).
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bioRxiv - Biophysics 2019Quote: ... The relative amount of present HRP was detected by adding 60 µL/well DPBS + 1 mM CaCl2 and 20 µl/well HRP substrate (Bio-Rad, 170-5060). The plate was incubated for 10 minutes at room temperature before recording of luminescence on an EnVision plate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
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bioRxiv - Cell Biology 2019Quote: ... cells from a 10 cm dish were pelleted and lysed with 30 µl lysis buffer (20 nM Tris-HCl, 150 mM NaCl, 1% Triton X-100 (Bio-Rad, Hercules, CA), 20 mM beta-glycerol phosphate and protease inhibitors cocktail ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Cell Biology 2022Quote: 100 microlitres of the secreted fractions and 100 µl of 1:20 diluted (in PBS) lysates from the secretion assays were loaded into a bio-blot microfiltration blotting system (BioRad. Cat. Nº: 1706545). Samples were left to flow through a 0.45 µm nitrocellulose blotting membrane (Amersham Protran ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Neuroscience 2022Quote: ... macrophages were plated in a 24-well plate containing cover slips and incubated with the Magic Red cathepsin B substrate (1/250) (Bio-Rad, ICT938) for 2h ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... aliquots of the samples were organized in a 96-well PCR plate and reverse transcribed to cDNA using the iScript™ Reverse Transcription Supermix kit and protocol (Bio-Rad Laboratories, Hercules, CA). The resulting 96-well plate with cDNA was used as source quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... were run on 4-20% polyacrylamide gels (Biorad #4561096) in 1 x Tris/Glycine/SDS buffer (Biorad #161-0772) ...
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bioRxiv - Physiology 2021Quote: ... or Mini PROTEAN TGX precast 4-20% (Bio-Rad). Proteins were then transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: 4-20% Mini-PROTEAN Gels (Bio-Rad, Cat. # 4561096)
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bioRxiv - Microbiology 2020Quote: ... into a 4-20% Mini-PROTEAN TGX Gel (Biorad) and run at 100 V for approximately 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on 4-20% TGX gels (BioRad) and proteins were transferred to a nitrocellulose membrane using semidry transfer system ...
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bioRxiv - Biochemistry 2022Quote: ... After SDS-PAGE (4-20% Protean precast gel, BioRad) and transfer to a PVDF membrane (Merck Millipore) ...
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bioRxiv - Biochemistry 2022Quote: ... Buffer B supplemented with 0.05% Tween 20 (BIO-RAD) was used as a running buffer throughout SPR measurements ...
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bioRxiv - Microbiology 2022Quote: ... and loaded on a 4-20% gel (Bio-Rad). Proteins were transferred to a PVDF membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... and by 4-20% SDS-PAGE gels (Bio-Rad) stained with Biosafe Coomassie (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: ... 4-20% SDS polyacrylamide gel electrophoresis (PAGE) (Bio-rad) and eletro-transfer at 100V for 2h were performed to separate protein extracts and transfer them to PVDF membranes (Bio-Rad) ...