Labshake search
Citations for Bio-Rad :
251 - 300 of 9568 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were washed using a 96-well plate magnetic handheld washer from Bio-Rad (NSW, Australia). The Bio-Plex Manager 3.0 software was used to operate the system and interpret the data.
-
bioRxiv - Microbiology 2020Quote: ... samples were boiled (10 min) and loaded (20 µL) on 15-well 4-20 % acrylamide precast gradient gels (Biorad). Supernatant-alone samples were similarly boiled and loaded on a cast 10% acrylamide gel ...
-
bioRxiv - Neuroscience 2021Quote: ... 10-20 µg of total protein was loaded on precast SDS-PAGE gels (4-20% Criterion TGX, Bio-rad) and immobilon-FL membranes (Merck Milipore ...
-
bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
-
bioRxiv - Neuroscience 2021Quote: ... Thirty or 20 microgramms were respectively loaded on 4-20% precast gel (Mini-Protean TGX precast gels, Bio-Rad) for APP and tau proteins ...
-
bioRxiv - Microbiology 2020Quote: ... Equal quantities of protein (generally 20-50 μg) were resolved on 4-20% gradient SDS-Polyacrylamide gels (Bio-Rad) and then transferred onto Polyvinylidene difluoride membranes (Millipore Sigma) ...
-
bioRxiv - Microbiology 2022Quote: ... 20 µg of samples were loaded into a 4–20% Mini-PROTEAN pre-cast gel (Bio-Rad, Cat: 4561093) and ran at 70 V for 30 min ...
-
bioRxiv - Genetics 2020Quote: ... Whole cell lysate protein samples (20-50 µg) were resolved on Criterion 4-20% gradient denaturing gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... Equal mass of each cell extract (20 μg) was separated on a 4-20% Mini-PROTEAN TGX gel (BioRad) and then transferred to Immobilon P membrane (Millipore ...
-
bioRxiv - Neuroscience 2022Quote: ... 10% gels with 50 μl wells were loaded with 20 μl CSF and 20 μl zymogram sample buffer (BioRad). Samples were separated by electrophoresis and gels were then extracted and incubated for 30 minutes in renaturation buffer (Novux ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein samples were run on 4-20% precast polyacrylamide gel (4-20% Mini-PROTEAN TGX Precast Protein Gels, BioRad) to separate them and transferred onto PVDF membranes using semi-dry transfer ...
-
bioRxiv - Cancer Biology 2023Quote: ... 20 μg of protein was loaded on a Mini-PROTEAN TGX gel 4-20% gradient pre-cast gels (BioRad). Protein was transferred to 0.45 μm nitrocellulose membrane (Biorad ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 μL of the supernatant was loaded onto a 4-20% Mini-PROTEAN Tris-glycine gradient gel (Bio-Rad) and proteins separated by SDS-PAGE were transferred to a PVDF membrane ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20-30μg protein samples were loaded into 4-20% Mini-PROTEIN TGX Precast Protein Gels (Bio-Rad, Cat# 4561096), and then were transferred to 0.2µm nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Cancer Biology 2019Quote: ... Collected sera were subjected to ELISA by capturing with human anti-rituximab idiotype antibody (HCA186, Bio-Rad Laboratories), detected with rat-anti-rituximab-HRP antibody (MCA2260P ...
-
bioRxiv - Microbiology 2021Quote: ... All serum samples were confirmed as Dengue virus-positive by means of NS1 diagnostic ELISA test (Platelia, Biorad). Serum samples were filtered using Millipore 0.22 μm PES syringe filters ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were determined using Bradford Assay Kit 1 (Bio-Rad) and 30µg of protein were loaded onto 8% SDS-Page Gels (Thermo Scientific) ...
-
bioRxiv - Microbiology 2019Quote: ... Alternatively broken cyst walls either before or after digestion with trypsin were reconstituted in 1× reducing SDS/PAGE loading buffer and run on a 4–20% precast polyacrylamide TGX gel (Bio-Rad). Bands stained by colloidal Coomassie blue were excised and washed with 50 mM NH4HCO3/acetonitrile (ACN) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Aqueous droplets with a 20-µl volume containing final concentrations of 1× Droplet Digital PCR Supermix for probes (Bio-Rad Laboratories) and 100 ng of the genomic DNA template were generated ...
-
bioRxiv - Neuroscience 2021Quote: ... of each sample was loaded on an 8-16% (for TSPO, 3b-HSD, and StAR) or 4-20% (for mERα, caveolin-1, and PKA) SDS precast gels (Bio-Rad) and separated by electrophoresis ...
-
bioRxiv - Neuroscience 2021Quote: ... for 10 minutes followed by 15 minutes of tris-buffered saline with 0.25% Triton X-100 then blocked for 1 hour in tris-buffered saline with Tween 20 solution at 0.05% (BioRad #161-0781) supplemented with sterile 2% Normal Donkey serum (Abcam #ab138579 ...
-
bioRxiv - Plant Biology 2021Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α and PP2A as housekeeping genes ...
-
bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...
-
bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
-
bioRxiv - Microbiology 2020Quote: ... A total of 100μL competent cells were incubated in a 1mm cuvette with 1–8μg DNA for 20 min on ice prior to pulsing in a GenePulser Xcell™ electroporator (Bio-Rad) with time constant and voltage settings at 5 ms and 1200V ...
-
bioRxiv - Cell Biology 2022Quote: ... The cDNA was diluted 1:20 prior to detection of GADD34 pre-mRNA transcripts using iTaq Universal SYBR Green (Bio-Rad). In short ...
-
bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution in PBS with 1% (w/v) skimmed milk and 0.1% (v/v) Tween-20 or goat anti-porcine IgA HRP (Bio-Rad Antibodies) at 1:20,000 dilution in the same diluent.
-
bioRxiv - Genomics 2020Quote: ... FLAG immunoprecipitates were eluted at 95°C for 10 minutes and GFP immunoprecipitates were eluted at 60°C for 20 minutes into 1 X SDS-Page Buffer (Bio-Rad) supplemented with 5% BME ...
-
bioRxiv - Plant Biology 2022Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α (5’-CTG GAG GTT TTG AGG CTG GTA T-3’ and 5’-CCA AGG GTG AAA GCA AGA AGA-3’ ...
-
bioRxiv - Microbiology 2022Quote: ... the generated cDNA was diluted (1:20) and amplified using stat1a- or stat1b-specific primers and 2x SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) in Hardshell® Low Profile Thin-wall 96-well skirted PCR plates (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 μL re-suspended Fraction B pellets were run 1 cm onto an Any kD Mini-Protean TGX gel (Bio-Rad) followed by Coomassie Blue staining ...
-
bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) was carried out using 4 μl cDNA (diluted 1:20) with iQ SYBR Green Supermix (BioRad, CA, USA) and specific primers (Biolegio ...
-
bioRxiv - Bioengineering 2022Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
-
bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
-
bioRxiv - Molecular Biology 2024Quote: ... 15 µL of each sucrose gradient fraction was incubated at 95 ºC with 1 % sodium dodecyl sulfate (SDS) and applied for SDS-PAGE with a 4-20 % gradient SDS-PAGE gel (Bio-rad). The proteins were transferred to a nitrocellulose membrane (Cytiva ...
-
bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
-
bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesised using the Bio-Rad iScript™ cDNA Synthesis Kit (25 x 20 µl reactions #1708890, Bio-Rad Laboratories Inc., USA), while the qPCR reaction was performed using the Applied Biosystems PowerUp™ SYBR™ Green Master Mix (A25741 ...
-
bioRxiv - Biochemistry 2024Quote: ... The total protein concentration of pooled saliva samples was measured to be approximately 20 mg/mL using the Bradford assay kit (Bio-Rad, Hercules, CA, USA) and bovine serum albumin (BSA ...
-
bioRxiv - Molecular Biology 2021Quote: ... triplicates of each sample were run per plate (Hard-shell PCR Plates 96 well, thin wall; Bio-Rad), which were sealed with Microseal ‘B’ Seals (BioRad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... triplicates of each sample were run per plate (Hard-shell PCR Plates 96 well, thin wall; Bio-Rad), which were sealed with Microseal ‘B’ Seals (BioRad) ...
-
bioRxiv - Microbiology 2020Quote: ... Amplification reactions of each DNA sample were performed in PCR plates (hard-shell PCR plate, #HSP9645; Bio-Rad), in a volume of 25 μl containing 12.5 μl PCR buffer (HotstarTaq mastermix ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
-
bioRxiv - Genomics 2020Quote: 20 μg of protein extracts in Laemmli buffer were run on a 4-20% gradient pre-cast gel (Bio-Rad) and transferred to nitrocellulose using Turbo transfer packs (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
-
bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Cell Biology 2020Quote: ... 20 μg of protein was loaded on 4-20% Mini-PROTEAN TGS Precast Protein Gels (Cat. No. 4561094, Bio-Rad) from each condition and ran at 100 V for 1 hour followed by transferring the protein on a PVDF membrane ...
-
bioRxiv - Neuroscience 2021Quote: ... The samples were then loaded (15-20 μg of protein per well) in a 4–20% Precast Protein Gel (Biorad) and resolved by SDS-polyacrylamide gel electrophoresis ...
-
bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: ... The membrane was subsequently washed 3 times in TBS+0.05 % Tween-20 over 45 min and incubated with secondary antibodies for 1 h at RT (20 °C) after washing 3 times in TBS+0.05 %Tween-20 and developed by enhanced chemiluminescence (Bio-Rad) with Image Quant™ LAS 4000.
-
bioRxiv - Cancer Biology 2022Quote: ... 20 μL of cell lysates were then loaded into precast 4-20% polyacrylamide SDS-PAGE gels (Bio-Rad, Catalog #: 5671094) for electrophoretic separation ...