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Citations for Bio-Rad :
4601 - 4650 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA (1 ug RNA per reaction) using the iScript cDNA Synthesis Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix and amplification was run on a CFX96 thermal cycler (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... and complementary DNA was reverse transcribed from 1 μg total RNA using iScript cDNA Synthesis Kit (Bio-Rad). mRNA expression was measured using iQ SYBR Green on a CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, CA, USA). In Quantitative real-time PCR (qPCR) ...
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bioRxiv - Plant Biology 2024Quote: ... Purified RNA (1 μg) was used for reverse transcription using the iScript™ cDNA Synthesis Kit (BIO-RAD). RT-qPCR was performed using SYBR green (ThermoScientific ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (1 μg) was used to generate the ProtoScript II First Strand cDNA Synthesis Kit (Biorad E6560S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: 1 μg TURBO DNase-treated RNA was reverse transcribed with the iScript cDNA reverse transcription kit (Biorad, 1708890) or 5 U AMV-RT (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from total RNA (1-2 μg) with iScript Advanced cDNA Synthesis Kit (Bio-Rad; 1725038). Quantitative RT-PCR (qPCR ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was reversely transcribed from 1 µg of RNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891BUN) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μg of RNA was reverse-transcribed (RT) to cDNA using iScript cDNA synthesis kit (Bio-Rad) with oligo-dT primers for poly-A mRNA selection ...
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bioRxiv - Genetics 2023Quote: ... 1 µg of total RNA was reverse transcribed to cDNA by using an iScript kit (#1708891, Bio-Rad) on a Mastercycler (Eppendorf) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 µg of RNA per sample using the iScript cDNA Synthesis Kit (Bio-Rad) or iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µg of RNA was used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
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bioRxiv - Microbiology 2023Quote: ... TCA precipitated protein mixtures were dissolved in 30 µL 1 x Laemmli sample buffer and loaded on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories, Germany). After short 3 cm SDS PAGE separation ...
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bioRxiv - Microbiology 2023Quote: ... was collected from each sample and transferred to 96-well ELISA plates for the measurement of the absorbance at 595 nm by using the iMarkTM Microplate Absorbance Reader (Bio-Rad, Alfred Nobel Drive. Hercules, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... On-bead ELISA readouts were measured using the standard PE and APC detection filter parameters on a ZE5 flow cytometer (Bio-Rad; 405, 488, 561, and 640 lasers) in the Scripps Research Flow Cytometry Core Facility ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of total protein mite extracts was determined using the Quick Start Bradford Protein Assay (Quick start Bradford 1x dye reagent, Bio-Rad, Cat# 500-0205), with Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Bradford Protein assay method was used to determine the protein concentration using bovine serum albumin (BSA) as the standard (Bio-Rad catalog 500-0006). 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 μg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Cell Biology 2021Quote: ... Sample proteins were extracted using RIPA buffer and the concentration protein in the supernatant was determined by the Bradford method (Bio-Rad Lab, California, USA). Similar amount of total cell lysate were fractionated through a 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Lab ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 µg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Molecular Biology 2019Quote: ... unbound and eluted proteins were precipitated by TCA extraction before running on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad #4561096) and western blotting was performed as described.
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Genetics 2020Quote: ... the soluble fraction was transferred to a new tube and total protein concentration was determined by DC protein assay (Bio-Rad, Hercules, CA, USA). Following quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... The fluorescence intensity was calculated relative to protein concentration in each sample as assessed by the DC™ Protein Assay (BioRad, Hercules, CA, USA) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: Approximately 15 μg of synaptosome protein was separated on a precast gradient gel (4-15% Mini-PROTEAN® TGX™ Precast Protein Gels, 4561086, BioRad, United Kingdom) and transferred to nitrocellulose membrane (Amersham™ ProtranÒ Western Blotting Membrane ...
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bioRxiv - Microbiology 2024Quote: ... Elution fractions of protein purifications were analyzed on 12 % SDS-PAGE gels and the protein concentrations were determined by Bradford (Bio-Rad Laboratories, Hercules, California) or Qubit protein assay (Thermo Fisher Sceintific ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... separated by electrophoresis and transferred to nitrocellulose membrane accordingly.1 The molecular mass of protein was determined by comparison with the migration pattern of Precision Plus Protein™ Prestained Standards Dual Color (Biorad Laboratories, CA, USA). The primary antibodies used were anti-pAKT (S473) ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein levels were normalized to the unphosphorylated version of the protein or the total protein content per lane as assessed via stain-free TGX gel technology (Bio-Rad, Hercules, CA, USA) [49] ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting proteins were separated by polyacrylamide gel electrophoresis (4%–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, Bio-Rad, California, USA) and then transferred to a nitrocellulose membrane (Reference ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was used as cell extract for further experiments after determination of the protein concentration using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2019Quote: ... All qPCRs were conducted in PrimeTime Gene Expression Master Mix (IDT) using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Canada, Montreal, QC). Each 15 µL qPCR reaction contained 0.5 µM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg of this plasmid DNA was transfected into the Pf NF54 ring stage parasites vie electroporation at 310 V and 950 μF by using a Bio-Rad Gene Pulser II (Bio-Rad Laboratories, Hercules, CA) and selected using 8 nM WR99210 (kindly gifted by Jacobus Pharmaceuticals) ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System; Bio-Rad; 250 V, 500 μF, 0.4-cm cuvettes). Cells were subsequently plated on MEF feeder layers (DR4 MEFs for puromycin or neomycin selection ...
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bioRxiv - Synthetic Biology 2019Quote: The concentrated mixed DNA from above was combined with 250 μL of electrocompetent yeast placed into a Gene Pulser Cuvette (BIO-RAD, catalog 3165-2086) prechilled in ice and then electroporated using a Bio-Rad Gene pulser XCell with the following settings ...
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bioRxiv - Bioengineering 2020Quote: ... This mix was transferred to a pre-chilled 2 mm electroporation cuvette and electroporation was performed (2.0 kV, 25 μF, 200 Ω) using a Gene Pulser Xcell (Bio-Rad Laboratories, Hercules, CA) electroporation sys-tem ...
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bioRxiv - Microbiology 2021Quote: ... For 16S rRNA gene analysis the 20 μl dPCR pre-droplet mix consisted of 10 μl dPCR Supermix for Probes (Bio-Rad Laboratories, Munich, Germany), 1 µl 20x 16S SNP Primer mix (final concentrations 900 nM) ...
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bioRxiv - Microbiology 2021Quote: ... Relative genome copy numbers were calculated based on the standard curve determined for each gene within CFX MaestroTM software (Bio-Rad, Hercules, CA, USA), and expression levels of the genes tested were normalized to the housekeeping gene GAPDH ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 50 ng cDNA from each sample was mixed with gene-specific primers (Supplementary Table 7) and SsoAdvanced™ universal SYBR® Green supermix (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... The reverse transcription- quantitative PCR (RT-qPCR) was performed to analyze the gene expression profile using SYBR® Green Supermixes (Bio-Rad, CA, USA) and CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Neuroscience 2020Quote: ... 6μL sterile water and 1μL of 300 nM intron-spanning gene specific forward and reverse primers in a Bio-Rad CFX96 Real-Time System (Bio-Rad, Hercules, CA, USA). Quantification of transcript levels were performed by comparing the threshold cycle for amplification of the unknown to those of six concentrations of standard cDNAs for each respective transcript ...