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Citations for Bio-Rad :
4501 - 4550 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a nitrocellulose membrane (1704271, Biorad) using a Trans-Blot Turbo system (Biorad) ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... and protein concentrations determined by Bio-Rad DC Protein assay (Bio-Rad, #500-0113, -0114, -0115). After equalizing protein concentrations with ddH2O ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was measured by Bradford assay (Bio-Rad) and 80-100 μg of total protein was used per lane ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred onto a PVDF membrane (Bio-Rad) using a semi-dry transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... protein samples were transferred to PVDF membranes (Bio-Rad) through a Trans-Blot TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were assessed using Bio-Rad BioPlex assays in both plasma and PFC homogenates (Th1/Th2 rat cytokines Bio-Rad, #171k1002M), according to manufacturer’s protocols ...
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bioRxiv - Biophysics 2021Quote: ... and transfected with Fluorescence Multiplex-Host Cell Reactivation (FM-HCR) reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad, #165-2670) using conditions 260 V ...
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bioRxiv - Physiology 2021Quote: ... mRNA expression analysis was performed using an iQ SYBR Green Supermix or Taqman Gene Expression Assays with an iQ5 thermal cycler and detection system (Bio-Rad, Hercules, CA). Hypoxanthine guanine phosphoribosyl transferase (Hprt ...
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bioRxiv - Plant Biology 2020Quote: ... were introduced into Agrobacterium strains EHA101, EHA105 and EHA105D by electroporation as described previously (Mattanovich et al., 1989) using a Bio-Rad Gene Pulser (Bio-Rad, CA, USA). After electroporation ...
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bioRxiv - Microbiology 2020Quote: ... was electroporated into cells at 5×106 cells/0.4 ml under conditions of 220V and 950 µF using a Gene Pulser™ (Bio-Rad, Hercules, CA) and plated on DMEM containing 10% FBS.
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bioRxiv - Cell Biology 2022Quote: ... Square wave electroporation was performed using a Gene Pulser Xcell Eukaryotic System installed with the Capacitance Extender and Pulse Controller modules (BioRad #1652661, BioRad #1652668). Our electroporation protocols were optimized using pulse duration ...
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bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
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bioRxiv - Neuroscience 2022Quote: After 9-13 days in culture, organotypic hippocampal slices were transfected biolistically with gene gun (McAllister, 2000) using gold beads (Bio-Rad, 1.6 µm) coated with plasmids ...
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bioRxiv - Plant Biology 2019Quote: ... Gene expression of three biological replicates was examined on MYiQ Single-Color Reverse Transcriptase-Quantitative PCR Detection System (Bio-Rad, Hercules, CA, USA) using TB Green™ Premix ExTaq™ II (Takara ...
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bioRxiv - Immunology 2019Quote: ... Total gene expression levels were assessed through a quantitative real-time PCR (qRT-PCR) using a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA) and SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... BDNF exon IV and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA (as a reference gene) were amplified using the iScript RT-PCR iQ SYBR Green Supermix (BIORAD, Hercules, CA, USA) in a real-time quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2021Quote: ... exponentially growing host cells were transferred to shallow plastic dishes and subjected to UV irradiation at 254 nm for 30 s using a BioRad GS gene linker (BioRad, Hercules, California, USA). Third ...
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bioRxiv - Cell Biology 2019Quote: ... A WT culture synchronized for 5% ring stages was loaded with 100 µg vector in transfection buffer via electroporation (310 V, 950 µF, 10 ms; Bio-Rad gene-pulser) as described (Ngwa et al. ...
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bioRxiv - Genomics 2021Quote: ... Four biological and three technical replicates for each gene were analyzed using a CFX96 Real-Time PCR detection system (BioRad, Mississauga, ON, Canada). Malus domestica Actin (MdACT ...
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bioRxiv - Microbiology 2021Quote: ... and transformed with plasmids by electroporation using Bio-Rad Gene Pulser (0.1-cm gap cuvettes, 1.6 kV, 200 Ω, 25 μF) (Bio-Rad, Hercules, CA, USA) following the method developed for Z ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were transfected with in vitro-transcribed RNA transcripts by electroporation with a Gene Pulser Xcell system (1.5 kV, 25µF and 200Ω) (Bio-Rad, Hercules, California, United States). At 22 h post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection was achieved by electroporation at 450 μFD and 240 V at room temperature (Gene Pulser Xcell, Bio-Rad Laboratories GmbH, München, Germany) in a 4 mm electroporation cuvette ...
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bioRxiv - Plant Biology 2022Quote: ... with 200 nM of each gene-specific forward and reverse primer (Supplemental Table S1) mixed with SYBR® Green supermixes (Bio-Rad, USA) according to the manufacturer’s instructions on a Thermal Cycler CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... The transcript levels of selected genes were measured by RT-qPCR using the CFX96® Real □ Time PCR System (BioRad, http://www.bio□rad.com/) and the 2 × T5 Fast qPCR Mix (SYBRGreenI ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products (700 ng) were introduced into electrocompetent cells of JW0452 carrying pKD46 by electroporation using a Gene Pulser XcellTM (BioRad, Hercules, CA, USA). The occurrence of recombination at the expected site was confirmed by PCR using primers CAT-584 and BW25113_2815723f (Table 2) ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Biochemistry 2024Quote: Quantitative real-time PCR was conducted to assess the relative expressions of genes involved in phenylalanine metabolism and terpene biosynthesis with a Bio-Rad CFX96 instrument (Bio-Rad, Shanghai, China). The gene-specific primers used for qRT-PCR was listed in Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The assembled products were mixed with electrocompetent cells and transformed into electrocompetent cells in a 2-mm Gene Pulser cuvette (Bio-Rad, 165-2086) using an electroporation instrument (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 200 µL of competent cells were mixed with 4 µg of library plasmids and then subject to electroporation (1000V, 50μF, 200Ω) using a Gene Pulser Xcell microbial electroporation system (Bio-Rad, Hercules, CA, USA). With this setting ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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bioRxiv - Bioengineering 2023Quote: ... were irradiated using the pre-programmed sterilisation setting (maximum wavelength of 253.7 nm, 90 seconds exposure) on the GS Gene Linker UV chamber (Bio-Rad laboratories, California, USA). Samples placed in petri dishes received three repeats of irradiation for top and bottom surfaces of the dish ...
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bioRxiv - Molecular Biology 2023Quote: ... The Au-RNA particles were then used for bombardment onto the oat plants using the Helios Gene Gun (Bio-Rad, Hercules, CA, USA) at a pressure setting of Hpsi=200 ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspension was transferred to a 0.2 cm gap cuvette and immediately electroporated by GENE PULSER II (Bio-Rad, Hercules, CA, USA) at 0.45 kV and 50 μF ...
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bioRxiv - Neuroscience 2023Quote: The reference gene used in combination with the designed human PMP22 assay was the commercially established AP3B1 (dHsaCP1000001; #10031245, Bio-Rad, Basel, Switzerland) ddPCR copy number variation assay.
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bioRxiv - Plant Biology 2023Quote: ... cDNA was diluted 10 fold in nuclease free water and reactions were performed in a Bio-Rad CFX96 system using gene specific primers (Table S1) and Bio-Rad SYBR green master mix (Bio-Rad, Hercules, USA). Each sample was run in triplicate ...
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bioRxiv - Plant Biology 2024Quote: ... The hpRNAi clones of pART27 were finally transformed into Agrobacterium tumefaciens strain EHA105 by electroporation (25 µF, 200 Ω, 2500 V) in a Gene Pulser XCell (Bio-Rad, USA).
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bioRxiv - Microbiology 2024Quote: ... blaOXA-48) and the intI1 gene by PCR methods 20-23 (Supplementary tables S1 and S2) in a Thermocycler (BIO-RAD T100™) using specific primer sets (Supplementary table S2) ...
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bioRxiv - Microbiology 2024Quote: ... and used as a template for amplification cycles conducted on a Corbett Life Science Rotor-Gene® 6000 thermal cycler with SsoAdvanced universal SYBR green supermix (Bio-Rad Laboratories). The reference gene was nadB [54] ...
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Progerin Can Induce DNA Damage in the Absence of Global Changes in Replication or Cell ProliferationbioRxiv - Molecular Biology 2024Quote: ... The cell/DNA mixture was then electroporated in a 0.4 cm gap electroporation cuvette using a Bio-Rad Gene Pulser (Bio-Rad, Hercules, CA, USA) at 700 V and 25 μF ...
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Progerin Can Induce DNA Damage in the Absence of Global Changes in Replication or Cell ProliferationbioRxiv - Molecular Biology 2024Quote: ... 5 × 106 cells were mixed with 20 μg of pSce in a volume of 800 μl of phosphate buffered saline at room temperature and electroporated in a 0.4 cm gap electroporation cuvette using a Bio-Rad Gene Pulser (Bio-Rad, Hercules, CA, USA) set to 700 V and 25 μF ...
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bioRxiv - Microbiology 2024Quote: ... and R-tot (5′-CTGCTGCCTCCCGTAGGAGT-3′) in the Rotor Gene 6000 thermocycler (Corbett Research, Sydney, Australia) with the iTaq Universal SYBR Green Supermix (Bio-Rad, Reinach, Switzerland).
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bioRxiv - Cell Biology 2020Quote: ... 40 μg of whole cell protein lysate were applied to 4%-15% Mini-PROTEAN TGX precast protein Gel (Bio-Rad, Cat No. 4561084) with electrophoresis and then transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2021Quote: ... Total protein concentration of the supernatant was measured using the Bradford assay (Bradford Reagent Protein Assay Dye Reagent, Bio-Rad, cat. number 5000006). Unboiled protein samples were then mixed with 4x Laemmli sample buffer and 100 mM dithiothreitol (DTT) ...
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bioRxiv - Biochemistry 2020Quote: ... The B4GAT1 activity then was normalized against the amount of protein measured in the 10 µL of sample using the DC Protein Assay (Bio-Rad, Hercules, CA) with BSA as the standard ...
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bioRxiv - Immunology 2021Quote: ... the cell lysate in the sample buffer was loaded with equal amounts of protein into the wells of an Any kD mini-PROTEAN protein gels (Bio-Rad, Hercules, CA). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were centrifuged at 12,000g for 20 mins to remove cell debris and protein concentration in supernatants was determined using DC™ Protein Assay (Bio-Rad, #5000111). Twenty μg of proteins from L ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay, Bio-Rad, Richmond, CA) (Lowry et al ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yields were between 8 and 12 mg/L. Proteins were analyzed by SDS-PAGE (Supplementary Fig. 2a) using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). The gels were run at 200 V for 30 min.
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bioRxiv - Cell Biology 2021Quote: Total cell lysates were prepared in Laemmli SDS-sample buffer supplemented with Protease Inhibitor Cocktail (Nacalai Tesque, Kyoto, Japan) and protein concentrations were quantified by the DC protein assay (Bio-Rad, CA, USA). Protein concentrations were normalized if necessary and boiled for 5 min with 2-mercaptoethanol ...