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Citations for Bio-Rad :
4451 - 4500 of 7745 citations for 7 Methyl 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a nitrocellulose membrane for 2 hours in a wet blot tank system (Bio-Rad), and membranes were blocked with 5% skim milk for 30 min at room temperature before incubating with primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... using primers specific for fathead minnow β-actin (Table 2) with iTaq universal SYBR green supermix (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... IPG strips were run upon SDS PAGE (12.5% acrylamide) using the Protean® II 2-D Cell (BioRad). Gels were then Coomassie blue stained (Phastgel Blue R ...
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bioRxiv - Bioengineering 2022Quote: ... ddPCR reactions were set up as follows: 11 μL 2×ddPCR™ supermix for probes (no dUTP) (BioRad), 0.2 μL FWD primer (100 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products were confirmed by 2% agarose gel electrophoresis stained with GelRed using a 50 bp marker (BioRad). PCR products were normalized using Mag-bind Total Pure NGS ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×106 cells (extracted tissues or cell culture) were suspended in 50 μL RIPA (Radio ImmunoPrecipitaion Assay; BioRad) lysis buffer containing 200 mM PMSF (Alpha-Phenyl Methyl Sulfonyl Fluoride ...
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bioRxiv - Genomics 2022Quote: ... These products were analyzed on a 2% agarose gel and was imaged with Gel Doc imaging system (BioRad). The observation of multiple bands implied successful targeting of the sequences by the sgRNA.
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bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed on 2 μl with the SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and the following primer pairs ...
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bioRxiv - Systems Biology 2019Quote: ... 272 mM sucrose) were mixed with 2 µg of DNA in a 1mm gapped electroporation cuvette (Bio-Rad). Cells were incubated on ice for 15 min and electroporated using the Gene Pulser XCell™ electroporation system (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... The resulting cDNA was PCR amplified and run on a 2% Certified Low Range Ultra Agarose (Bio-Rad) gel with subsequent extraction using the QIAquick® Gel Extraction Kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... and then the WBC solutions were embedded into 2% agarose plugs (CHEF Genomic DNA Plug Kit, Bio-Rad) to avoid fragmentation of long DNA molecules ...
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bioRxiv - Developmental Biology 2020Quote: ... Smit1/2 and TonEBP were measured by real time qPCR using SYBR Green I (Bio-Rad, Mississauga ON) and 5 μM of forward and reverse primer mix (Bio-Rad CFX384 ...
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bioRxiv - Genetics 2021Quote: ... The enriched proteins were dissociated by the addition of 2 x Laemmli sample buffer (161-0737, Bio-Rad) and boiled at 95 °C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were dried for 2 h at 80°C and analysed on a Personal Molecular Imager (Bio-Rad). For RNA stability assays ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 50 μM oligo dT primer (Bio-Rad, 1725038) and 1 μl of 25 mM dNTP mix (NEB ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 μl of the cDNA sample were supplemented with 5 μL of SsoADV Universal SYBR Green Supermix (BioRad) mix 2X ...
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bioRxiv - Genetics 2019Quote: ... the size of the amplicon was determined by comparison with a 2-kb DNA marker with the use of Quantity One software (version 4.6.7; Biorad), and the number of repetitions was estimated by comparing the size of the product amplified from the Nichols strain ...
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bioRxiv - Plant Biology 2022Quote: ... For α-ACTIN-2 the secondary antibody Goat Anti-Mouse IgG (H + L)-HRP conjugate (1706516, Bio-rad) was used at a dilution of 1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Captured proteins were separated from beads by incubating beads in 50 μl 2× Laemmli Sample Buffer (Bio-Rad) at 95°C for 25 min ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were added with equal volume of 2× Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) containing 65.8 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA Synthesis Kit (Bio-Rad). A 1/10 dilution of the cDNA was then used for qRT-PCR analysis utilizing the SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was passed twice on 2 x Mini CHT ™ type I column (Bio-Rad, United States). Elution was performed with a gradient of 0 to 200 mM NaPO42- in 10 min ...
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bioRxiv - Physiology 2023Quote: ... Protein samples were suspended in Laemmli Sample Buffer supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Then ...
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bioRxiv - Immunology 2022Quote: ... 200 μL of this transformation mix was then aliquoted into pre-chilled 2 mm electroporation cuvettes (Bio-Rad) and electroporated at 1500 V with an average time constant of ∼4.5 ms using a Gene Pulser Xcell Electroporation System (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... unreacted ITC-DTPA was removed by dialysis against 0.25 M ammonium acetate (NH4Ac) pH 5.4–5.5 (metal free) supplemented with 2 g/L Chelex (Bio-Rad) using 20,000 molecular weight cut-off dialysis cassettes (Slide-a-Lyzer ...
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bioRxiv - Physiology 2023Quote: ... and samples of four larvae each were homogenized in 100 μl 2× SDS sample buffer (Bio-Rad #1610737) containing 5% (355 mM ...
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bioRxiv - Plant Biology 2023Quote: ... 2 ug RNA was used to synthesize cDNA with the iScript™ cDNA Synthesis Kit (Bio-Rad 1708890). The SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 1725271 ...
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bioRxiv - Molecular Biology 2023Quote: ... heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Three hundred micrograms of protein was precipitated using a ReadyPrep 2-D Cleanup Kit (Bio-Rad Laboratories, USA) at −20 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The detergent was removed by three 2-hour incubations with ∼33 mg of Bio-Beads SM2 (Bio-Rad) followed by overnight incubation with ∼50 mg of Bio-Beads SM2 with all incubations occurring at 4°C.
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bioRxiv - Neuroscience 2023Quote: ... cDNA synthesis was performed using 2 μg of total RNA and the iScript cRNA synthesis kit (Bio-Rad). qPCR was performed using the Brilliant III ultra-Fast SYBR green qPCR master mix (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... The mixture was transferred to an ice-cold 2 mm gap electroporation cuvette (Bio-Rad Laboratories GmbH, Germany), and cells were electroporated with a single pulse at 1.8 kV ...
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bioRxiv - Genomics 2023Quote: ... Gene panel probe hybridization occurred overnight for 18 hours at 50°C (Bio-Rad DNA Engine Tetrad 2). Subsequent washes the next day removed unbound probes ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
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bioRxiv - Microbiology 2024Quote: ... and 2 µL of each reaction was mixed with 10 µL of 1x Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... at 45 V for 2 h using the Mini-PROTEAN Tetra Vertical Electrophoresis Cell System (Bio-Rad, 1658029FC) and the blotting buffer containing 0.1 M glycine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 25 μL of lysate mixed with sample buffer (1:1 ratio) (Bio-Rad) was loaded per lane in a 10% SDS-PAGE gel (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... and rat anti-F4/80 (1:500, Clone A3-1, MCA497GA, Biorad, USA). After 3×5 min washes ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... Phalloïdine 647N ATTO (1/200) and DAPI nuclear dye (Bio-rad,1/1000) were diluted in the blocking solution ...
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2x Laemmli Buffer (Bio-Rad Laboratories, 161-0737). 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were mixed 1:1 with 2X Laemmli sample buffer (Bio-Rad) with 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and rabbit anti-alpha actinin-1 at 1:1000 (Bio-Rad Laboratories #VPA00889). Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted proteins were separated by NuPAGE 4-12% Bis-Tris gel in MES SDS running buffer and transferred to a PVDF membrane (Bio-Rad, 162-0263). Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... 40 μg of whole cell protein lysate were applied to 4%-15% Mini-PROTEAN TGX precast protein Gel (Bio-Rad, Cat No. 4561084) with electrophoresis and then transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were separated on 6-10 % SDS-polyacrylamide gels or 4-20 % Mini-PROTEAN TGX precast gradient gels (Bio-Rad, CA, USA) together with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... The purity and integrity of the proteins was evaluated for each batch by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-15% gradient gels (Mini-PROTEAN® TGX® Precast Protein Gels, Bio-Rad) with a Precision Plus Protein® Kaleidoscope® (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty micrograms of protein were loaded on 4-15% gradient TGX Stain-Free™ Protein Gels and transferred to Immun-Blot® LF PVDF membrane (Bio-Rad). The membranes were blocked with 1X TBS with 0.05% Tween 20 and 5% skim milk powder for 2 hours at 25°C ...
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bioRxiv - Microbiology 2020Quote: The samples used for immunoblot analysis were separated by SDS-PAGE on a 4-20% gradient gel (Mini-PROTEAN TGX Gels, Biorad, Cat# 456-1093). Gel electrophoresis was performed at 120 V in running buffer (Tris-glycine ...