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Citations for Bio-Rad :
4701 - 4750 of 7745 citations for 7 Methyl 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... TGN46 (BioRad, AHP500, 1/100), YBX1 (ProteinTech ...
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bioRxiv - Cell Biology 2024Quote: ... CD68 (Biorad MCA1957GA; 1:200), CD206 (Abcam ab64693;1:200) ...
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bioRxiv - Cell Biology 2023Quote: ... TGN46 (Biorad, AHP500GT, 1:200), washed 3 times with PBS and incubated in blocking buffer for 45 min with fluorescently labelled secondary antibodies at 1:400 ...
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bioRxiv - Immunology 2023Quote: ... F480-PB (CI:A3-1)(BioRad), (v ...
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bioRxiv - Cancer Biology 2023Quote: ... in 1×TBS (BioRad #1706435)/0.1% Tween 20 (Bio-Rad #161-0781 ...
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bioRxiv - Cancer Biology 2023Quote: ... #0500-0099 (1/50000, Biorad). Proteins were enhanced by chemiluminescence reagent (Biorad ...
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bioRxiv - Immunology 2023Quote: ... (CD68, 1:200, BioRad, MCA1957T) and (IgA ...
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bioRxiv - Physiology 2024Quote: ... CD68 (1:500, rat, Biorad) PSD95 (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... 5μg of either whole brain lysate or tubulin-enriched protein fraction was loaded per lane and run on 4-20% Mini-Protean TGX Stain-Free precast gels (4568093; Bio-Rad Laboratories, Hercules, CA) at 150mV for 1hr ...
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bioRxiv - Cell Biology 2020Quote: Experiments were run using 26-well gels (Criterion TGX Precast Gels, 15 μl, 1.0 mm, 4-20%, Bio-Rad Laboratories, Inc. Cat No 5671095). 13 wells were loaded by one researcher and the other set of 13 wells were loaded by another researcher for each set of experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was diluted 18-fold and 4 µL was used in a reaction for SYBR green detection with SsoAdvanced SYBR Green supermix (Bio-Rad, catalog no. 1725270) on a CFX384 machine (Bio-rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was diluted 20-fold and 4 µL was used in a reaction for SYBR green detection with SsoAdvanced SYBR Green supermix (Bio-Rad, catalog no. 1725270) on a CFX384 machine (Bio-rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reducing SDS sample buffer was added and proteins resolved on 4%-15% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad, California, United States) or 3%-8% NuPAGE Tris-Acetate Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of the resulting solution was then applied to either 4-12% Criterion™ XT Bis-Tris Protein Gels (BioRad, Hercules, CA, USA) or NuPAGE™ 4 – 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... and one-dimensional electrophoresis was carried out for 4 hours at 75-100 volts in a Tris-glycine-SDS (Bio-Rad, catalog 161-0732) running buffer ...
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bioRxiv - Physiology 2020Quote: ... and equal amounts of total protein (15 or 20 μg) were loaded in different wells on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad, Australia). A stain-free system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 μg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and electroporated in a 4-mm cuvette using an exponential pulse at 250 V and 950 mF in a Gene Pulser electroporation device (Bio-Rad Laboratories, Feldkirchen, Germany). We used a plasmid encoding a CMV-mCherry-Cas9 expression cassette and a human SAMHD1 gene specific gRNA driven by the U6 promoter ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: ... Protein extracts were mixed with loading buffer containing SDS and dithiothreitol (DTT) and loaded onto Mini-PROTEAN® TGX™ Precast Gels (4-15% gradient; Bio-Rad 4561084) in Tris-Glycine buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels, Biorad, Hercules, CA) and transferred to nitrocellulose (GE healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were solubilized in Laemmli sample buffer and heated at 95°C for 10 min to denature proteins and separated via SDS-PAGE for 40–60 min at 270 V using 4–20% stain-free gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Thereafter ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 µg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Molecular Biology 2019Quote: ... unbound and eluted proteins were precipitated by TCA extraction before running on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad #4561096) and western blotting was performed as described.
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bioRxiv - Neuroscience 2021Quote: ... These samples were run on a 4–20% SDS-PAGE and electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories, Hercules, CA, USA) from the gel by a semidry blotting apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Bioengineering 2021Quote: ... incubated for 5 min at 95°C and used as template in the qPCR reaction in a final 10−4 dilution with the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The qPCR was run in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Immunology 2020Quote: ... of protein were incubated in 1X Laemmli buffer with 0.1 M dithiothreitol at 65°C for 15 min and electrophoresed on 4-15% gradient TGX gels and transferred to PVDF membranes (Bio-Rad Laboratories, Mississauga, Canada). The membranes were blocked with Tris-buffered saline with 0.05% Tween 20 (TBST) ...
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bioRxiv - Biophysics 2020Quote: ... Pure ClyA-AS type-I (12-mer) nanopores were obtained using native PAGE on a 4-15% gradient gel (Bio-Rad, Veenendaal, The Netherlands) and subsequent excision of the correct oligomer band.
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from recovered fractions were analysed by Western blotting (4-15% Mini PROTEAN® TGX Stain-Free™ Gels & Trans-blot® Turbo™ Nitrocellulose Transfer Pack, BioRad). The bait proteins were probed using a His Tag HRP-conjugated antibody (Ozyme ...
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins or whole cell lysates were separated by SDS-polyacrylamide gel electrophoresis on a 4-20% gradient gel (Bio-Rad Laboratories, Hercules, CA) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts (50 μg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Biochemistry 2021Quote: ... Premade 4-20% TGX gels with either the 10-well 50 μL or 15-well 15 μL formats (Bio-Rad cat. # 4561094 and 4561096) were used in all instances ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 µg of each protein mixture was separated by SDS-PAGE (polyacrylamide gel electrophoresis) on a Criterion XT 4-12% Bis-Tris gel (Biorad Laboratories cat. No. 3450124). Proteins were transferred to a PVDF membrane (Merck Millipore cat ...
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad Mini-PROTEAN® TGX™ 4-15% Gel) according to manufacturer’s protocols for electrophoresis at 110 V for 70 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Bead pellets were resuspended and boiled in β-mercaptoethanol containing Laemmli sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2022Quote: ... The radio-labelled proteins were then separated with SDS-PAGE on a 4-12% Criterion XT Bis-Tris gels (Bio-Rad, Hercules, CA, USA) in MES buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins in the lysates were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis using gradient (4–20 %) PAGE gels (Bio-Rad, Mini-PROTEAN TGX), with a molecular mass marker (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... 30μg protein for iPSC-derived optic cups or 15μg for iPSC-LESCs were loaded onto 4-15% Mini-PROTEAN ® TGX ™ gels (BioRad Inc., CA, USA) and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded onto 4-12% Criterion XT precast gels and proteins were separated by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electroblotted onto a 0.45 μm nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... by tank blotting at 250mA for 90 min in a cold room (4 °C) in blotting buffer (catalog#1610734, Bio-Rad, Hercules, CA, USA). The membrane was blocked for 2 h in TBS-T (tris buffered saline supplemented with 0.1 % Tween ...
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bioRxiv - Immunology 2022Quote: The purity and quality of the proteins were characterized at all stages of the purification process using 4%-20% SDS–PAGE (Bio-Rad, Catalog number 4561096) and 3%-12% Bis-Tris gel (ThermoFisher ...