Labshake search
Citations for Bio-Rad :
4301 - 4350 of 4352 citations for Human Complement C3 Convertase C3c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 1 uL of the extracted genomic DNA were used for qPCR and HRM analysis using the IQ SYBR® Green Supermix kit (BioRad, Cat no. 1708882). Mutation positive male sperm were used to fertilize wildtype eggs which were saved in culture beakers and raised until next spawn ...
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bioRxiv - Plant Biology 2023Quote: ... The qPCR was performed using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo-scientific) with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Hercules, CA, USA) according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... The aqueous layer containing RNA was then mixed with isopropanol and loaded to Aurum Total RNA Mini Kit columns (Bio-Rad, Mississauga, ON, Canada). Total RNA was then extracted according to the manufacturer’s instructions and concentration was assessed on the nanodrop attachment for the Varioskan LUX Multimode Microplate reader (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... The viral genome of the H7N1 virus was quantified using qRT-PCR that was conducted with the Bio-Rad iTaq™ Universal SYBR ® Green One-Step Kit (BioRad, California, USA) using primers designed for the detection of the M gene as published elsewhere 60.
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bioRxiv - Plant Biology 2023Quote: ... One microgram of high-quality RNA was used for the first-strand cDNA synthesis by using an iScript™ cDNA synthesis kit (Bio-Rad Laboratories, USA). Primer specificity was ensured by performing a BLASTN search of each sequence against the Solanum lycopersicum ITAGv2.40 database (Supplementary Table S1) ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... Mini kit (Macherery-Magel, Germany) and used to prepare complementary DNA (cDNA) using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Hercules, CA, USA) on ABS Proflex Thermal cycler (Thermo-fisher ...
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bioRxiv - Physiology 2024Quote: ... Purified RNA was then used for cDNA synthesis via reverse transcription as per protocol by iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). All PCR reactions (25 µL ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using 100 ng of purified total RNA in a 20 μL volume reaction with an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA), as described in (52) ...
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bioRxiv - Genetics 2024Quote: ... The membranes were then incubated with ECL (Easysee Western Blot Kit, China) and visualized with an Imaging System (Bio-Rad, Universal Hood II, USA).
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bioRxiv - Microbiology 2024Quote: Proteins separated by SDS-PAGE were transferred to a PVDF membrane using the Trans-Blot® Turbo RTA Mini PVDF Transfer Kit (Bio-Rad Laboratories, Inc.). Membranes after activating with methanol were blocked with bovine serum albumin (Amresco ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of RNA were then used for cDNA synthesis with the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, catalog number 1725038). RT-qPCR analysis was performed on the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad). Antibody detection was carried out with the SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Precision Plus Protein™ Dual Color Standards and Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit were purchased from Bio-Rad (Hercules, CA). Antibodies against Phospho-c-Raf (Ser338) ...
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bioRxiv - Cell Biology 2019Quote: ... and then converted to units of fmol/mg of total protein added to an assay point (as measured by Bio-Rad DC Protein Assay Kit). Equilibrium dissociation constants (Kd ...
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Glutathione S-transferase: A Candidate Gene for Berry Color in Muscadine Grapes (Vitis rotundifolia)bioRxiv - Genetics 2020Quote: ... Total RNA from berry skins and seeds was used for the synthesis of first strand cDNA using iScript cDNA synthesis kit (Bio-Rad Laboratories Inc., Hercules, CA) and diluted 1:10 for qPCR reactions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 1st cDNA was synthesized from 200 ng of total RNA of stomach origin by using oligo-dT primers and an iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). The sequences of primers for cDNA cloning were designed based on the genome resource (see Table 2) ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA was synthesized from total RNA with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher. Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Biorad; Cat No. 1725121) or SYBR Green PCR master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... 100 ng aliquots of RNA before and after depletion were subjected to qPCR using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Hercules, CA) [22] ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated by reverse transcription of the total RNA samples with iScriptRT Advanced cDNA Synthesis Kit for RT-PCR (Bio-Rad, Hercules, CA; #170–8891;). qRT-PCR was performed in triplicates using the iTaqTM Universal SYBR® Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: Commercially available TaqMan assays (Table S2) for droplet digital PCR were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Hercules, CA, USA; 1864022). Each 20-μL reaction mix consisted of 10 μL ddPCR Supermix for probes (no dUTP ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved in 10% SDS-polyacrylamide gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit (#1704271 from Bio-Rad Laboratories, Hercules, CA, USA). Phosphorylation of MAPKs Slt2 and Fus3 was detected using a rabbit antiPhospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Neuroscience 2020Quote: ... Uptake was normalized to total mg of protein per sample measured via Bio-Rad DC protein assay kit (5000112 Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Cell Biology 2019Quote: ... Gels were transferred using Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit and the Trans-Blot® Turbo™ Transfer system (Bio-Rad). Membranes were blocked with Odyssey® Blocking Buffer (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... aliquots of the samples were organized in a 96-well PCR plate and reverse transcribed to cDNA using the iScript™ Reverse Transcription Supermix kit and protocol (Bio-Rad Laboratories, Hercules, CA). The resulting 96-well plate with cDNA was used as source quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Genetics 2021Quote: ... equal amounts of cDNA were synthesized from 300 ng of total RNA for each sample by using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Thirty nanograms of cDNA per reaction were analyzed for semi-qPCR using the SsoAdvanced™ Universal SYBR® Green Supermix Kit (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: Total RNA from snap-frozen IECs was isolated with peqGOLD RNAPure™ (Erlangen, Germany) and reverse transcription was performed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, California, USA). Following ...
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bioRxiv - Immunology 2021Quote: ... For T84 cells: 250 ng of total RNA was reverse transcribed into cDNA using the iScript™ cDNA Synthesis kit (BioRad Laboratories, Catalog number 1708891). The reaction contained a mixture of 1 μl Reverse Transcriptase ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Physiology 2022Quote: mRNA expression was analyzed by quantitative RT–PCR as previously described (49) using cDNA synthesis kits and SYBR green mix from Bio-Rad (Cat# 1708897 and 1725121) performed with the StepOne Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Viral genome was quantified using a standard curve of known viral titer following qRT-PCR with iTaq Universal SYBR Green One-Step Kit (BioRad, cat#172-5151, Hercules, CA) and the following primers ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA synthesis of 500 ng of RNA in a final volume of 20 μL was performed using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated with Omni-ECL™Femto Light Chemiluminescence Kit (Epizyme, China) and captured using the Molecular Imager® Gel Doc™ XR System (Bio-Rad, USA). The antibodies used were as follows ...
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bioRxiv - Molecular Biology 2023Quote: To prepare the tubing for gene gun delivery one tubing and 25 mg of Au particles with 1 µm Ø was used from Helios Gene Gun Optimization Kit (Bio-Rad, Hercules, CA, USA). 10 µg of the synthesized RNA transcript was used four labeling Au particles ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations were analyzed using a Quick Start Bradford Protein Assay Kit with bovine serum albumin as a standard (Bio-Rad Laboratories, Hercules, CA, USA). The samples were standardized by diluting in the diluent ...
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bioRxiv - Microbiology 2024Quote: ... Bio-Rad MyiQ was used to quantify the DNA concentration of the library using the Kapa iCycler qPCR kit (Bio-Rad Laboratories, Hercules, CA, USA). Sequencing was performed on the Illumina NextSeq 500 Platform (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting total protein was quantified using the Bio-Rad DC Protein Assay Kit according to the manufacturer’s recommended protocol (Bio-Rad, Cat#5000111, Hercules, CA, USA). Samples and standards were read on a BioTek Synergy HT plate reader after a 15 min incubation period ...
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bioRxiv - Neuroscience 2024Quote: mRNA expression was analyzed by quantitative RT–PCR as previously described using cDNA synthesis kits and SYBR green mix from Bio-Rad (Cat# 1708897 and 1725121) performed with the StepOne Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative PCR was performed using a KOD SYBR qPCR kit (TOYOBO) on a CFX96 Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA, USA). PCR conditions were as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... Equal amounts of RNA for all samples in Fig 3N were simultaneously transcribed into cDNA using iScript cDNA synthesis kit (BIO-RAD, Hercules, CA, cat#170-8891) according to the manufacturer’s protocol within 7 days of the initial dissections to preserve sample quality ...
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bioRxiv - Cancer Biology 2020Quote: ... blots were developed using an ECL Ultra kit (New Cell & Molecular Biotech Co. Ltd, China) and images were captured using a ChemiDocTM XRS + Imager (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels of above-mentioned genes were estimated using iTaq™ Universal SYBR® Green One-step qRT-PCR kit (#1725151, Bio-Rad Laboratories Inc., USA) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... This sequence was amplified from B6J genomic DNA extracted from spleen tissue using iProof High-Fidelity PCR kit (BIO-RAD, Hercules, CA, USA; Cat# 172-5330) with the following primers containing XhoI and HindIII restriction enzyme sites in the sense and antisense primers respectively ...
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bioRxiv - Plant Biology 2019Quote: ... qrt-PCR reaction was prepared using 2x SensiMix™ SYBR & Fluorescein Kit (PEQLAB LLC, Wilmington, DE, USA) and PCR was conducted with a combination of iCycler and IQ5 (Bio-Rad Laboratories, Inc., Hercules, CA, USA). All transcript levels were normalized to the tubulin β gene (AT5G62690 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from the RNA according to the Bio-Rad iSCRIPT cDNA kit protocol using a Bio-Rad thermocycler (Bio-Rad Laboratories, Inc.; Hercules, CA, USA). After obtaining cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... The reverse transcription of the RNA was performed by using the iScript™ cDNA synthesis Kit (Cat. No. 1706691; Bio-Rad Laboratories AG, Hercules CA, USA). Relative mRNA expression levels were evaluated using the SYBR Green method ...
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bioRxiv - Cell Biology 2024Quote: ... RNA concentrations were measured by Nanodrop and 100ng of total RNA where subjected to first-strand cDNA synthesis with iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA, 170-8891). cDNA was amplified using the Luna Universal qPCR Master Mix (New England Biolabs M3003E ...
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bioRxiv - Biochemistry 2023Quote: ... following gel electrophoresis proteins were transferred from the gel to a polyvinylidene fluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad, Trans-Blot turbo RTA transfer kit) using the Bio-Rad standard protocol (25 V,30 min) ...
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bioRxiv - Biochemistry 2022Quote: ... and 1.0 μg of RNA per sample was reverse-transcribed was reverse transcribed using the iScript™ cDNA synthesis kit from Bio-Rad (Bio-Rad, Hercules, CA). Quantitative PCR was performed using SensiFAST SYBR Hi-ROX Kit in 25 µL reaction volume (Meridian Bioscience ...