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Citations for Bio-Rad :
3951 - 4000 of 4352 citations for Human Complement C3 Convertase C3c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized in duplicate using 500 ng RNA in 10 uL iScript Advanced cDNA Synthesis Kit (Bio-Rad, 172-5038). Reactions were diluted 1:5 in water ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was collected and quantified using the Bio-Rad Protein Assay Kit and the Quick Start Bovine Serum Albumin Standard Set (Bio-Rad) and stored at −80°C before use.
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bioRxiv - Microbiology 2023Quote: ... The membranes were then rinsed with TBST as above before chemiluminescence detection using the Clarity Western ECL substrate kit (cat. no. 1705060, Bio-Rad). Gels and blots were recorded with a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... diluted to 1 ng/μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Pathology 2023Quote: ... The immune complexes were detected using a chemiluminescence kit and visualized via the ChemiDoc XRS Gel documentation system (Bio-rad, CA). The gray intensity was measured by the software of Quantity One (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated and purified from cultured cell lines using the Aurum Total RNA Mini kit (Bio-Rad, Cat# 7326820). RNA was converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: Whole cell and tissue protein was extracted using the Active Motif protein extraction Kit and quantified with the Bradford assay (Bio-Rad). Snap-frozen cardiac apical tissues were homogenized using Dounce tissue grinder (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... in SDS running buffer then blotted onto nitrocellulose membranes through Trans-Blot Turbo RTA Mini 0.2 µm Nitrocellulose Transfer Kit (Bio-Rad #1704270). Membranes were hybridized with 1:1000 dilution of the anti-Arm primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription to create complementary DNA (cDNA) with the iScript cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions in a total volume of 20 μl ...
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bioRxiv - Cell Biology 2023Quote: ... and cell death was performed using Annexin V-FITC/PI apoptosis detection kit following the protocol described by the manufacturer (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... The concentration of the isolated RNA was determined using Nanodrop and 250ng RNA was converted into cDNA using the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were developed using the ECL kit (Seven, Beijing, China) and detected using the ChemiDoc imaging system (Bio-Rad, California, USA).
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bioRxiv - Microbiology 2022Quote: ... The blots were then washed three times by PBST and then visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) by a ChemiDoc MP Imaging System (Bio-Rad).
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA Synthesis Kit protocol (Bio-Rad Laboratories Inc.; Hercules, USA), and the concentration was determined using a Qubit flex fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaq™ universal SYBR® Green One-Step kit (Bio-Rad): 95 ºC for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 4995 µL of cell lysis buffer and 20 µL of PMSF 500 mM (Bio-Plex® Cell Lysis Kit, Biorad, #171304011). The lysate was frozen at −80°C for 2 minutes and then ...
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bioRxiv - Immunology 2023Quote: ... we developed a digital droplet RT-PCR (RT-ddPCR) employing the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad) and the QX200 Droplet Digital PCR System (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from whole Drosophila animals and converted to cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, cat#1708891). Primers for qPCR were designed using IDT PrimerQuest (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentrations in each tube were measured by Bradford assay using Quick Start Bradford protein assay kit (Bio-Rad). An equal amount of each sample (30-35 μl) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: The bacterial and fungal loads in extracted DNA samples were quantified by RT-qPCR using the iTaq Universal SYBR Green One-Step kit (Bio-Rad). Each reaction was prepared in a final volume of 20 µL containing 5 µL of template DNA ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Biorad, #1725035; Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration of the supernatant was measured using the detergent compatible (DC Lowry) protein assay kit according to the manufacturer′s directions (Bio-Rad). For each sample ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 1 μg of total RNA using the Reverse Transcription system (iScriptTM cDNA synthesis kit, Bio-Rad). Quantitative Real time PCR was performed using Sybr Green Master Mix (SYBR Green master mix ...
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Custom primers were designed to meet several criteria using NCBI Primer Blast followed by running of the complementary DNA (cDNA) synthesis product made using RT-PCR (iScript kit, Cat no. 1708890, Biorad, USA) on an electrophoresis gel ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoreactivity signals were detected with the SuperSignal West Femto Maximum Sensitivity Substrate kit (Pierce) and a Chemico image system (Bio-Rad). Densitometry quantification of protein band intensities was carried out using ImageJ.
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bioRxiv - Physiology 2023Quote: ... diluted to 1 ng/ μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Cell Biology 2024Quote: ... RNA isolation was succeeded by cDNA preparation from 1µg of total RNA using iScript™ cDNA Synthesis Kit (#1708891; Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... All mRNAs were transcribed using the SP6 mMessage mMachine kit (Fisher Sci #AM1340) and purified using Biorad Microbiospin columns (Biorad #7326250). Two guide RNAs targeting chordin and Three guide RNAs targeting tyrosinase were transcribed using T7 RNA polymerase (NEB #M0251s ...
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bioRxiv - Cancer Biology 2024Quote: scATAC-seq libraries were performed with the Bio-Rad’s SureCell ATAC-Seq Library Prep Kit according to the manufacturer’s instructions (Bio-Rad, Cat# 17004620). Library concentration and fragment size were checked by Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of each total RNA preparation as template and the iScriptTM Reverse Transcription Supermix for qRT-PCR Kit (Bio-Rad). Primers listed in supplemental table S7 were employed for RT-PCR and qRT-PCR analysis of NtGEF12 transcript levels in comparison to reference gene expression ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... blots were developed using a SuperSignal West Pico plus chemiluminescence substrate kit (Thermo) and imaged using a ChemiDoc MP imaging system (Bio-Rad). Images were analyzed using Image Lab (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR were performed following the manufacturer’s instructions for the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) using 100 ng of total RNA as template ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with QIAquick® PCR purification kit and 1-10 ng of immunoprecipitated or input DNA were amplified with iQ™ SYBR Green Supermix (BioRad) using a Real-Time PCR machine (BioRad IQ™ 5) ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNAs were prepared in a 10μl volume containing 350 ng of plant total RNA that was reverse-transcribed with an iScriptTM cDNA Synthesis Kit using modified MMLV-derived reverse transcriptase (Bio-Rad), a blend of oligo-d(T ...
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bioRxiv - Systems Biology 2023Quote: We performed cDNA synthesis with 0.5-1 μg of total purified RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from PMVECs and Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA) following the manufactureŕs instructions ...
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bioRxiv - Genetics 2024Quote: 500 ng of RNA was converted to cDNA using the iScript cDNA synthesis kit (#1708891, Bio-Rad, Hercules, CA, United States) containing random hexamers according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin signal was detected and quantified by chemiluminescence according to standard LightShift Chemiluminescent RNA EMSA Kit protocol using a ChemiDoc™ Touch Imaging System (BioRad).
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bioRxiv - Genetics 2024Quote: ... and cDNA was generated from 0.5 micrograms of purified RNA from each sample using an iScript cDNA Synthesis Kit (Bio-Rad #1708890). qPCR was conducted using iTaq Universal SYBR Green Supermix (Bio-Rad #1725151) ...
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bioRxiv - Biophysics 2024Quote: ... A luminol-based chemiluminescence assay kit (Chemi-Lumi One L, Nacalai tesque) and chemiluminescence detection system (ChemiDoc Touch MP imaging system, Bio-Rad) were used to detect chemiluminescence signals.
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...