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Citations for Bio-Rad :
4001 - 4050 of 4472 citations for Rat Tsukushin Tsku ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription of 500ng of total RNA was performed using the iScript cDNA synthesis kit (Bio-Rad Laboratories, Inc, California, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1µg of each total RNA sample was used to synthesize cDNA using the i-Script cDNA synthesis kit (Bio-Rad, USA). The real-time PCR was performed using a 20µl reaction volume on 96-well plates by using i-Taq Syber Green (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Zoology 2022Quote: ... sfRNA and 3’UTR were quantified together by RT-qPCR using the iTaq Universal Sybr green one-step kit (Bio-Rad) with primers previously designed [26] ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were separated in 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane using a Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit (Bio-Rad). Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Microbiology 2022Quote: Cell pellets from 2 mL overnight cultures were used to create gel plugs using the CHEF Bacterial Genomic DNA Plug Kit (Bio-rad). DNA was digested with I-SceI ...
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bioRxiv - Microbiology 2022Quote: ... β-lactamase activities were normalized relative to total protein concentration measured using Pierce Coomassie Plus Assay Kit (Bio-Rad, CA, USA) to obtain the enzyme activity in units pr ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then performed quantitative polymerase chain reaction (qPCR) using 2 ng of complementary DNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quantity and quality was assessed using a NanoDrop spectrophotometer and cDNA was synthesized using an iScript cDNA Synthesis Kit (Biorad, 1708891) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 ng RNA (RIN>8) was transcribed to cDNA using the iScript first strand cDNA synthesis kit (Bio-Rad 1708891). qPCR was carried out on a 384-well Quantstudio Flex system (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using oligo-d(T) primers and avian reverse transcriptase (iScript cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... mRNA levels of SASPs (Figure 1e) were measured by RT-qPCR using iTaq Universal One-Step RT-qPCR Kit (Bio-Rad) with primer pairs previously described 67 ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of 1000 ng of RNA was used for cDNA synthesis using a High Capacity cDNA Reverse Transcription Kit (BioRad, #1708891). To detect the contaminated DNA we used the samples processed without the transcriptase reverse enzyme as negative controls ...
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bioRxiv - Zoology 2022Quote: ... RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad). Primers for five genes—Clock ...
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bioRxiv - Plant Biology 2022Quote: ... The chemiluminescent signals were developed by WesternBright ECL kit (LPS Cat# K-12045-D20) and documented on the ChemiDoc MP system (Bio-rad).
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bioRxiv - Plant Biology 2022Quote: ... WesternBright ECL kit was used to detect the chemiluminescent signals (LPS Cat# K-12045-D20) and ChemiDoc MP system (Bio-rad) was used to capture the image.
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bioRxiv - Microbiology 2022Quote: ... 600 ng in 15 μL was used for reverse transcription using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). The reverse transcription program was run as follows ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA) following the manufactureŕs instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... All mRNAs were transcribed using the SP6 mMessage mMachine kit (Fisher Sci #AM1340) and purified using Biorad Microbiospin columns (Biorad #7326250). Two guide RNAs targeting chordin and Three guide RNAs targeting tyrosinase were transcribed using T7 RNA polymerase (NEB #M0251s ...
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bioRxiv - Cell Biology 2024Quote: ... RNA isolation was succeeded by cDNA preparation from 1µg of total RNA using iScript™ cDNA Synthesis Kit (#1708891; Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of each total RNA preparation as template and the iScriptTM Reverse Transcription Supermix for qRT-PCR Kit (Bio-Rad). Primers listed in supplemental table S7 were employed for RT-PCR and qRT-PCR analysis of NtGEF12 transcript levels in comparison to reference gene expression ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression of TRAF6 was determined relative to GAPDH using iTaq Universal SYBR Green One step RT-qPCR kit (Bio-Rad) employing the Ct method as described in Bio-Rad RT-qPCR user manual.
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat # 5000111; BioRAD, Inc, Hercules CA) with bovine serum albumin as a standard according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from about 2000 L4 animals and reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was then analyzed on the CFX384 thermocycler (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by chemiluminescence using the Clarity Western ECL substrate detection kit (Bio- Rad) and a Chemidoc imaging system (Bio-rad). Whole proteins were detected on gel using the stain-free technology.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from mouse tissues using the Aurum Total RNA Fatty and Fibrous Tissue Extraction kit (Biorad: #732-6870). RNA from cells was extracted using Zymo Research R1055 Quick-RNA MiniPrep Kit ...
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bioRxiv - Cancer Biology 2024Quote: Serum cytokines were measured using the Luminex xMAP technology on the Bio-Plex 200 platform (Bio-plex Manager 5.0) with the Bio-Plex Pro™ Human Cytokine Screening Test Kit (48-Plex, Bio-Rad) and the Bio-Plex Pro™ Human Inflammation Panel-1 Kit (37-Plex ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the cDNA was used with the KAPA SYBR FAST Universal qPCR Kit (KAPA) and run on a CFX96 real-time PCR detection system (Bio-Rad). The qPCR primers are listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: scATAC-seq libraries were performed with the Bio-Rad’s SureCell ATAC-Seq Library Prep Kit according to the manufacturer’s instructions (Bio-Rad, Cat# 17004620). Library concentration and fragment size were checked by Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... blots were visualized using the LI-COR Odyssey CLx or the Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) and a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... the Bio-Rad ddPCR Supermix for Probes (no dUTP) kit was used to perform the reaction within droplets generated by the QX200 droplet generator (Bio-Rad) in Oil-for-Probes (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 hour of blocking in 5% milk ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...
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bioRxiv - Microbiology 2023Quote: ... Distinct MagPlex microsphere sets (Luminex Corp, Austin, TX, USA) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... and 1-1.5 µg RNA was used a template to make cDNA with the iScriptTM cDNA synthesis kit (Bio-rad #1708890), as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Molecular Biology 2023Quote: ... The digestion products were analyzed by 12-15% SDS-PAGE and stained with Coomassie blue or silver (Silver stain plus kit, Bio-rad). Images were taken using a Chemidoc imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of RNA was reverse transcribed to cDNA with the iScript cDNA Synthesis Kit following the manufacturer’s protocol (Bio-Rad, #1708891).
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bioRxiv - Developmental Biology 2024Quote: ... cDNA synthesis was performed using 900 ng of total RNA for hPSCs and 1000 ng of total RNA for EBs and the iScript Reverse Transcription Kit (BIO-RAD) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Only RNA with RIN value above eight was used for cDNA synthesis using the iScript cDNA synthesis kit (Biorad, Cat.No.1708891). RT-qPCR was then performed in a 96-well plate using the PowerUp SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from about 3000 L4 animals and reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was then analyzed on the CFX384 thermocycler (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: cDNA corresponding to rRNA in both the large and small size cDNA fractions were removed using the SEQuoia kit (Biorad, 17006487) according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was used in a reverse transcription reaction with the iScript cDNA Synthesis kit (Bio-Rad, 1708891) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR was performed with a SYBR Green PCR kit (Toyobo, Japan) in CFX Connect Real-time PCR detection system (Bio-Rad). The specific qPCR primers used are listed in Supplemental Table 2.
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bioRxiv - Cancer Biology 2023Quote: ... followed by bead washing with kit-included washing buffer and eluting with 20 μl 2x Laemmli sample buffer (Cat# 161-0737, BIO-RAD). A 20-50 μg sample for each group was saved for immunoblot quantitation of total Rac1 ...
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bioRxiv - Neuroscience 2023Quote: ... Ten µg of total proteins quantified by PierceTM BCA protein assay kit (ThermoScientific) were loaded on 4-15% gradient SDS–PAGE gels and transferred to nitrocellulose membranes (Trans-Blot turbo, Bio-Rad). Immunoblotting of rodent cultures was detected by chemiluminescence with clarity Western ECL on ChemiDoc Touch system with Image Lab software (Bio-Rad ...