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Citations for Bio-Rad :
3951 - 4000 of 4472 citations for Rat Tsukushin Tsku ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... the membrane was then developed by incubation with chemiluminescence (Amersham ECL prime kit) and imaged with a ChemiDoc™ XRS+ System (BIORAD). The intensity of GFP bands were quantified with Image J software ...
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bioRxiv - Bioengineering 2021Quote: ... Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves: an iScript cDNA synthesis kit (Bio-Rad) was used to reverse transcribe total RNA into cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Austria).52 The luciferase activity data was normalised to protein content measured by DC Protein determination kit (Bio-Rad Laboratories, Inc., USA) and SCO effect was expressed as a fold-change over untreated cells ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Immunology 2020Quote: ... the signals were detected using an enhanced chemiluminescence detection kit (Super Signal West Pico Plus, 34580, ThermoFischer Scientific) and membranes imaged with ChemiDoc Imaging System (Bio-Rad). The same anti-S1 and anti-S2 antibodies as well secondary antibodies were also used to detect the Spike protein on EVs by ELISA (see protocol below).
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bioRxiv - Molecular Biology 2020Quote: 500ng of genomic RNA were used for reverse transcription into cDNA using the iScript cDNA synthesis kit (Bio-Rad, #170-8891). cDNA was diluted 10X and 2uL was added for each qPCR reaction (iQ™ SYBR Green Supermix ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence substrate was then applied to the blot according to the manufacturer’s instructions (Bio-Rad Clarity™ Western ECL Substrate kit). The chemiluminescent signals were recorded using a Syngene G:BOX (Synoptics ...
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bioRxiv - Cancer Biology 2019Quote: Plasma activities of ALT and AST were assessed with the ALT and AST Reagent Kit (Biosystems Reagents) using a Benchmark Plus microplate spectrophotometer (Bio-Rad). Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme (ATAC Tagmentation Buffer and Enzyme are both included in the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad)) ...
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bioRxiv - Genomics 2021Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2021Quote: ... One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD, 1708840). qPCR reactions for targeted genes were performed with the Luminaris HiGreen qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Microbiology 2021Quote: ... total 600 ng of RNA was used for cDNA synthesis by using Random hexamer oligonucleotide primer (iScript Select cDNA Synthesis Kit, BioRad, USA). Gene specific primers (Table S2 ...
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bioRxiv - Genomics 2020Quote: ... The concentrations of cytokines in plasma were measured using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit immunoassay run on the Bio-Plex 200 (BioRad), with reference to an 8 point standard curve.
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNAs were synthesized from 500 ng DNase-treated total RNA by RT with the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 µL according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Serum was also collected for cytokine analyses which were performed by the University of Pennsylvania’s Human Immunology Core using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane by semi-dry transfer using the Trans-Blot turbo RTA Mini PVDF transfer kit (Bio-Rad) and the Trans-Blot turbo system ...
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bioRxiv - Physiology 2021Quote: ... was performed with the Bio-Rad iQ TM SYBR Green Supermix kit and data were analyzed with the CFX384 system (Bio-Rad). mRNA quantitation was done by normalizing the amount of RNA detected for the gene of interest ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Developmental Biology 2021Quote: ... eluted total RNA in 10 μl of Elution buffer and reverse transcribed RNA in 20 μl using the iScript cDNA synthesis kit (Bio-Rad). We incubated the samples for 5 min at 25°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA (1μg) of each postnatal stage was used for cDNA synthesis using a reverse transcription kit (iscript, Bio-Rad, USA). Real-time PCR amplification was performed using iTaq™ Universal SYBR® Green Supermix (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The antigen-antibody complexes were visualized using appropriate HRP conjugated secondary antibodies and enhanced chemiluminescence kit (ECL; Amersham or Bio-Rad) and normalization was reported to β-actin ...
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bioRxiv - Physiology 2021Quote: ... 5 ul of each supernatant sample was used to quantify the total amount of protein with a BCA Protein Assay Kit whereas the rest was diluted in 4x Laemmli sample buffer (Bio-Rad) with 2-Mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... markers was analyzed by qPCR as previously described (Yanai et al., 2013; Yanai et al., 2015) using an iScript cDNA synthesis kit (Bio-Rad) and TaqMan Primer/probe sets for each gene ...
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bioRxiv - Immunology 2020Quote: ... with 18S (Hs99999901_s1) as housekeeping control were carried out on RNA reverse transcribed to cDNA with an iScript cDNA synthesis kit (Bio-Rad), using Taqman chemistry and gene-specific probes purchased from Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: Total RNAs were extracted from cell-sorter purified cells using NucleoSpin RNA kit with a DNase treatment (Macherey Nagel) and reverse transcribed with iScript™ Reverse Transcriptase mix (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription and real-time PCR (qPCR) reactions were carried out using iScript cDNA synthesis kit and iQ SYBR Green Supermix (Bio-Rad). qPCR was performed on a Bio-Rad CFX384 Touch™ Real-Time PCR Detection System using the following human primers (Forward and Reverse ...
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bioRxiv - Immunology 2021Quote: Plasma from each patient sample was analyzed using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit (Bio-Rad). Cytokine expression was measured using the Luminex xMAP® (Luminex Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the RNA was assessed and first strand cDNA synthesised from 1 ug using the iScript Select cDNA Synthesis Kit (Bio-Rad) and a 1:1 mixture of Oligo (dT ...
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bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
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bioRxiv - Microbiology 2021Quote: RNA copies of SARS-CoV-2 samples were detected by quantitative real-time RT-PCR (RT-qPCR) assays were performed using the iTaq SYBR Green One-Step Kit (Bio-Rad) on the LightCycler 480 system (Roche ...
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bioRxiv - Immunology 2021Quote: ... Sera were collected for cytokines analyses which were performed by the Macau University of Science and Technology using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... According to kit instruction 10 μl reaction system was prepared and q-PCR was operated using CFX96 Real-Time System (BIO-RAD). For data analysis ...
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bioRxiv - Genetics 2020Quote: ... Equal amounts of DNA-free total RNA were converted to cDNA using the iScript™ cDNA synthesis kit (Bio-Rad #1708891). Quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
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bioRxiv - Microbiology 2020Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad). The results were analyzed using Bio-Rad CFX Manager software ...
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bioRxiv - Neuroscience 2021Quote: ... adult testes or hippocampus or cell pellets were homogenized in RIPA buffer and the protein concentration was determined using a Bradford Assay kit (Bio-Rad). For blot analysis ...
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bioRxiv - Neuroscience 2022Quote: RNA isolation for all tissues were carried out using the Aurum Total RNA isolation kit (BioRad #732-6820; Hercules, CA, USA). Samples were reverse-transcribed to cDNA using qScript cDNA Supermix (Quantabio #101414-106 ...
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bioRxiv - Immunology 2022Quote: ... Separated proteins were transferred to a PVDF membrane using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad Laboratories, Feldkirchen, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from the purified RNA by both random and oligo(dT) priming using the iScript cDNA synthesis kit (Bio-rad). RNA levels were measured by the SYBR Green relative quantitation method using either a 7300 or Viia 7 RT-PCR machine (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 μg of RNA was used to synthesize cDNA with Bio-RAD iScript cDNA Synthesis Kit (170-8891, Bio-Rad). qRT-PCR was performed using Taqman universal PCR mix and predesigned gene expression assays with best coverage from Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 3-4 μg of total RNA was reverse transcribed using the iScript advanced cDNA synthesis kit (Bio-Rad). Quantitative PCR of target genes (Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Genetics 2022Quote: Approximately 500 ng of total RNA was used to synthesize cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The HRM analysis was done according to the protocol provided by the Precision Melt Supermix Kit (Bio-Rad; Catalog #172-5112). The following oligonucleotides were used for the HRM analysis ...