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Citations for Bio-Rad :
4001 - 4050 of 4440 citations for Human Brain Derived Neurotrophic Factor BDNF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... RNA isolation was succeeded by cDNA preparation from 1µg of total RNA using iScript™ cDNA Synthesis Kit (#1708891; Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of each total RNA preparation as template and the iScriptTM Reverse Transcription Supermix for qRT-PCR Kit (Bio-Rad). Primers listed in supplemental table S7 were employed for RT-PCR and qRT-PCR analysis of NtGEF12 transcript levels in comparison to reference gene expression ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression of TRAF6 was determined relative to GAPDH using iTaq Universal SYBR Green One step RT-qPCR kit (Bio-Rad) employing the Ct method as described in Bio-Rad RT-qPCR user manual.
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat # 5000111; BioRAD, Inc, Hercules CA) with bovine serum albumin as a standard according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from about 2000 L4 animals and reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was then analyzed on the CFX384 thermocycler (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by chemiluminescence using the Clarity Western ECL substrate detection kit (Bio- Rad) and a Chemidoc imaging system (Bio-rad). Whole proteins were detected on gel using the stain-free technology.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from mouse tissues using the Aurum Total RNA Fatty and Fibrous Tissue Extraction kit (Biorad: #732-6870). RNA from cells was extracted using Zymo Research R1055 Quick-RNA MiniPrep Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the cDNA was used with the KAPA SYBR FAST Universal qPCR Kit (KAPA) and run on a CFX96 real-time PCR detection system (Bio-Rad). The qPCR primers are listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: scATAC-seq libraries were performed with the Bio-Rad’s SureCell ATAC-Seq Library Prep Kit according to the manufacturer’s instructions (Bio-Rad, Cat# 17004620). Library concentration and fragment size were checked by Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... blots were visualized using the LI-COR Odyssey CLx or the Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) and a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... the Bio-Rad ddPCR Supermix for Probes (no dUTP) kit was used to perform the reaction within droplets generated by the QX200 droplet generator (Bio-Rad) in Oil-for-Probes (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 hour of blocking in 5% milk ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...
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bioRxiv - Microbiology 2023Quote: ... Distinct MagPlex microsphere sets (Luminex Corp, Austin, TX, USA) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... and 1-1.5 µg RNA was used a template to make cDNA with the iScriptTM cDNA synthesis kit (Bio-rad #1708890), as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Molecular Biology 2023Quote: ... The digestion products were analyzed by 12-15% SDS-PAGE and stained with Coomassie blue or silver (Silver stain plus kit, Bio-rad). Images were taken using a Chemidoc imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of RNA was reverse transcribed to cDNA with the iScript cDNA Synthesis Kit following the manufacturer’s protocol (Bio-Rad, #1708891).
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bioRxiv - Developmental Biology 2024Quote: ... cDNA synthesis was performed using 900 ng of total RNA for hPSCs and 1000 ng of total RNA for EBs and the iScript Reverse Transcription Kit (BIO-RAD) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Only RNA with RIN value above eight was used for cDNA synthesis using the iScript cDNA synthesis kit (Biorad, Cat.No.1708891). RT-qPCR was then performed in a 96-well plate using the PowerUp SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from about 3000 L4 animals and reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was then analyzed on the CFX384 thermocycler (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: cDNA corresponding to rRNA in both the large and small size cDNA fractions were removed using the SEQuoia kit (Biorad, 17006487) according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was used in a reverse transcription reaction with the iScript cDNA Synthesis kit (Bio-Rad, 1708891) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR was performed with a SYBR Green PCR kit (Toyobo, Japan) in CFX Connect Real-time PCR detection system (Bio-Rad). The specific qPCR primers used are listed in Supplemental Table 2.
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bioRxiv - Cancer Biology 2023Quote: ... followed by bead washing with kit-included washing buffer and eluting with 20 μl 2x Laemmli sample buffer (Cat# 161-0737, BIO-RAD). A 20-50 μg sample for each group was saved for immunoblot quantitation of total Rac1 ...
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bioRxiv - Neuroscience 2023Quote: ... Ten µg of total proteins quantified by PierceTM BCA protein assay kit (ThermoScientific) were loaded on 4-15% gradient SDS–PAGE gels and transferred to nitrocellulose membranes (Trans-Blot turbo, Bio-Rad). Immunoblotting of rodent cultures was detected by chemiluminescence with clarity Western ECL on ChemiDoc Touch system with Image Lab software (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted using the Trizol method and cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Bio-Rad). qRT PCR analysis was performed in CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were recovered and protein concentrations were determined with the Bradford method (Protein Assay kit, Bio-Rad, Marnes-la-Coquette, France). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 µg RNA (sorted cells) or 1 µg RNA (tissues) was used to prepare cDNA with the iScript™ cDNA synthesis kit (Biorad) with the following protocol ...
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bioRxiv - Neuroscience 2023Quote: ... analysis was performed by reverse transcribing 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). iQ quantitative PCR was performed on the diluted cDNA following the manufacturer’s protocol (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was collected and quantified using the Bio-Rad Protein Assay Kit and the Quick Start Bovine Serum Albumin Standard Set (Bio-Rad) and stored at −80°C before use.
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bioRxiv - Neuroscience 2023Quote: cDNA was synthesized by conversion of 1 µg of the DNAse-I treated RNA with iScript Selected cDNA Synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then rinsed with TBST as above before chemiluminescence detection using the Clarity Western ECL substrate kit (cat. no. 1705060, Bio-Rad). Gels and blots were recorded with a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... One microgram of RNA per sample was reverse transcribed using the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad, Hercules, CA) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was transferred to a clean new tube and protein concentration was quantified with the DC Protein Assay kit (Bio-Rad). SDS-PAGE and western blot analysis were conducted as described (Tseng et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The signal was detected by chemiluminescence with Super Signal Kit (Pierce) and autoradiography with Hyblot CL films (Denville Scientific) or ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Evolutionary Biology 2023Quote: ... Quantitative real-time PCR analysis was performed with ChamQ Universal SYBR qPCR Master Mix kit (Vazyme) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) under the conditions of 95 °C for 2 min ...
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bioRxiv - Biochemistry 2023Quote: ... were resolved by SDS-PAGE in a 10 % v/v homemade polyacrylamide gel under reducing conditions and transferred to a nitrocellulose membrane by semi-dry transfer (kit #1704272, Bio-Rad). Membranes were saturated in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer from gels to polyvinylidene difluoride (PVDF) membranes was carried out using the Trans-Blot Turbo Transfer Kit (1704274, Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was first synthesised from the extracted RNA using the Bio-Rad iScript kit and a T100 Thermal Cycler (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized in duplicate using 500 ng RNA in 10 uL iScript Advanced cDNA Synthesis Kit (Bio-Rad, 172-5038). Reactions were diluted 1:5 in water ...
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bioRxiv - Cell Biology 2023Quote: ... diluted to 1 ng/μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated and purified from cultured cell lines using the Aurum Total RNA Mini kit (Bio-Rad, Cat# 7326820). RNA was converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: Whole cell and tissue protein was extracted using the Active Motif protein extraction Kit and quantified with the Bradford assay (Bio-Rad). Snap-frozen cardiac apical tissues were homogenized using Dounce tissue grinder (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... in SDS running buffer then blotted onto nitrocellulose membranes through Trans-Blot Turbo RTA Mini 0.2 µm Nitrocellulose Transfer Kit (Bio-Rad #1704270). Membranes were hybridized with 1:1000 dilution of the anti-Arm primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription to create complementary DNA (cDNA) with the iScript cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions in a total volume of 20 μl ...
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bioRxiv - Cell Biology 2023Quote: ... and cell death was performed using Annexin V-FITC/PI apoptosis detection kit following the protocol described by the manufacturer (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... The concentration of the isolated RNA was determined using Nanodrop and 250ng RNA was converted into cDNA using the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instruction ...