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Citations for Bio-Rad :
351 - 400 of 522 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: 40 μL of ssDNA-Cy5-SWNT in 0.1 M PBS and 10 μL of SC solution were added to a 96-well PCR plate (Bio-Rad) for a final concentration of 5 mg/L ssDNA-Cy5-SWNT and 0.1 wt.% SC ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined at indicated time points by adding 1/10 volume AlamarBlue solution (Biorad, Hercules, CA, United States) to each well ...
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bioRxiv - Genetics 2021Quote: ... unspecific binding sites were blocked over night at 4°C with 3% milk powder (Marvel) in Tris Buffered Saline solution (BioRad) including 1% Tween 20 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... MtCoaBC and MsCoaBC were exchanged into NH4OAc (500 mM, pH 7.0) solution using Micro Bio-Spin 6 chromatography columns (Bio-Rad). A sample volume of 2.5 μL was injected into a borosilicate emitter (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... samples were washed for two minutes with degassed polyacrylamide (PA) solution consisting of 4% (vol/vol) 19:1 acrylamide/bis-acrylamide (161010144, BioRad), 60 mM Tris-HCl pH 8 (AM9856 ...
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bioRxiv - Cell Biology 2021Quote: ... We incubated it for 30 minutes before adding the stop solution and reading the results in a plate iMark microplate absorbance reader (BioRad).
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bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... The following day the membrane was washed with PBST twice for 10 minutes and incubated with secondary antibodies diluted in the same solution (1:10000 HRP-conjugated anti-mouse or anti-rabbit antibodies (Biorad)) in the same solution at room temperature for 45 minutes ...
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bioRxiv - Biophysics 2021Quote: ... This solution was stirred at room temperature for 30 min followed by addition of 7.5 mg BioBeads (BioRad, Hercules CA) and a further 90 min incubation ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were then treated with HRP-conjugated goat anti-moue secondary antibody (1:10000) diluted in blocking solution for 1 h and developed with enhanced chemiluminescent (ECL) substrate (Cat#1705060, Biorad).
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bioRxiv - Microbiology 2020Quote: ... 0.05 % [w/v] Coomassie R) staining solution or blotted on polyvinylidenfluorid (PVDF) membranes using the Trans-Blot Turbo Transfer System (BioRad). As positive controls purified FtsZ1 or FtsZ2 was loaded on the respective gel ...
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bioRxiv - Genomics 2020Quote: ... Membranes were washed using tris-based saline buffer solutions with 0.1% tween-20 (TBST) and blocking solutions were prepared using 5% w/v western-blot grade dry milk powder (Biorad). Membranes were blocked with blocking buffer for 1 hour and washed between incubations for 5-10 mins (3x) ...
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bioRxiv - Biochemistry 2021Quote: ... The secondary antibody was rinsed off by washing the blots again with TBST for three times and developed with Promega ECL solution on chemidoc (BioRad). Blots were stripped with low pH stripping buffer and then re-probed for total RSK using RSK1/RSK2/RSK3 rabbit monoclonal primary antibody at 1:2500 dilution (CST ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were washed again with TBST for three times and developed with Promega ECL solution (Cat. no. W1015) on chemidoc (BioRad). Blots were stripped with low pH stripping buffer and then re-probed for total ERK using rabbit total ERK (CST ...
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bioRxiv - Cell Biology 2022Quote: ... pre-wet nitrocellulose in TBS solution (150 mM NaCl, 50 mM Tris pH 7.4) was inserted into a slot blot apparatus (Bio-Rad) connected to vacuum aspiration ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies (for further specification see key resource table, table 1) were diluted in blocking solution (F4/80 [Bio-Rad] ...
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bioRxiv - Bioengineering 2020Quote: 50 μL of PCR mastermix solution in Nuclease-free ultrapure water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
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bioRxiv - Bioengineering 2020Quote: ... 50 μL of PCR mix solution in nuclease-free water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with Amersham ECL Western Blotting Detection Reagent (Global Life Science Solutions) for 1 min and imaged on a ChemiDoc Imaging System (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with the washing solution twice and then reacted with 4-chloro-1-naphthol (Bio-Rad Laboratories) in the HRP color development buffer (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2021Quote: ... The solution was then transferred to a tube containing ∼30- 50 mg of Bio-Beads (Bio-Beads SM-2, BioRad) that were previously equilibrated in 20 mM Tris-HCl pH 7 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were centrifuged at 3000g and the pellet was washed with ice-cold water twice before resuspending in 10% glycerol solution for electroporation using a GenePulser XCell (BioRad) electroporator with the pre-set bacterial protocol for E ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were stripped using Restore™ Western Blot Stripping Buffer (Cat. number: 21059) following the manufacturer’s protocol and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 200 µL of bead mix solution were added to all wells and washed using a Bio-Plex Pro Wash Station (BIORAD). 25 µL of Universal Assay Buffer were added to all wells ...
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bioRxiv - Immunology 2021Quote: ... Viable and dead lung cell counts were determined using 0.4% Trypan Blue solution and a TC10™ Automated Cell Counter (BioRad). BAL samples were centrifuged and supernatants from the first BAL retrieval harvested ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 15 minutes in 1x TBST solution and incubated for 5 minutes at room temperature with either Clarity Western ECL (BioRad) or Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PFGE was undertaken by running samples on a 1.0% agarose gel in 0.5 X TBE solution at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, BioRad). 6 V/cm were used with 15 h switch time of 60 s followed by 15 h 120 s at 120° ...
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bioRxiv - Biophysics 2022Quote: ... Any residual BioBeads were removed by passage of the solution through a Micro Bio-Spin chromatography column (0.8 ml; Bio-Rad). The proteoliposome sample in the 2 ml Eppendorf tube was placed on ice in preparation for the lipid flipping assay.
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bioRxiv - Cell Biology 2022Quote: ... CDP-star substrate solution was added to the membrane before visualization using either X-ray films or ChemiDoc Touch Imaging System (BioRad). Quantification of the signal intensity was done using Fiji (ImageJ) ...
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bioRxiv - Cancer Biology 2023Quote: ... A volume of 30 μL of resulting solution was run on an SDS-PAGE gel and imaged using the ChemiDoc MP System (BioRad). Imaging was followed by Coomassie Blue staining for total protein quantification.
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bioRxiv - Cell Biology 2023Quote: Polyacrylamide gels of 7.9 kPa stiffness were made by adjusting acrylamide and bisacrylamide stock solution (Bio-Rad Laboratories, Hercules, CA) concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was cut lengthwise into strips to isolate bands of particular molecular weights before being washed with TBST and incubated at room temperature for 1 H in a 5% blocking buffer solution (Biorad). The western blot strips were then incubated overnight at 4 C in a solution consisting of the primary antibody diluted into blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated with BioRad clarity western ECL substrate and Millipore HRP substrate solutions and then imaged in a ChemiDoc MP from BioRad. Some blots were then stripped with 25 mM glycine-HCl and 1% SDS (pH 2.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell counts were measured in triplicate using a TC20 Automated cell counter (BioRad) (15 µl cell suspension + 15 µl Trypan Blue Dye (0.4% solution, BioRad #1450013), 10 µl was transferred to each chamber in a cell counting slide with dual chambers (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... the bacteria were resuspended in 100 µL of 300 mM sucrose solution and transferred to a Gene Pulser Cuvette (0.2-cm electrode gap, Bio-Rad), and 100 ng of plasmid DNA (pEB1GM or pEB2GO ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Biophysics 2023Quote: ... The blots were finally washed thrice with TBST with 5 minute incubations before adding the ECL substrate solution (1705061, Biorad). Chemiluminescence images were acquired on a Chemidoc™ imaging system (Biorad) ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was rinsed three times for 10 minutes in TBS followed by the addition of 1 mL ECL solution (BioRad) to the membrane and visualized on the ImageQuant LAS 500.
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bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 10 minutes with 1x TBST solution followed by 5 minutes incubation at room temperature with either Clarity Western ECL (BioRad). Immunoblots were developed using an Amersham ImageQuant 500 CCD imaging system (Cytiva) ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
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bioRxiv - Neuroscience 2022Quote: ... 20% DMSO and 0.3M Glycine for 1h followed by a blocking step using an aqueous solution of 0.2% Triton X100 (Bio-Rad Laboratories), 0.2M Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: ... The combined solution was incubated at room temperature for 1 hr and then mixed with BioBeads SM-2 resin (BioRad) overnight to remove the detergents ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were then visualized using Pierce Enhanced Chemo-Luminescence solution and imaged in a Biorad Chemi-Doc chemoluminescence system (BioRad).
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bioRxiv - Neuroscience 2023Quote: ... The blots were then visualized using Pierce Enhanced Chemo-Luminescence solution and imaged in a Biorad Chemi-Doc chemo-luminescence system (BioRad).
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with Amersham ECL Western Blotting Detection Reagent (Global Life Science Solutions) for 1 min and imaged on a ChemiDoc Imaging System (BioRad).
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bioRxiv - Cell Biology 2024Quote: ... blots were incubated in 1-shot ECL solution (Kindle Biosciences LLC) and imaged with a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Cell Biology 2024Quote: ... The blots were incubated with either with anti-HA or anti-ubiquitin antibodies followed by probing with luminol solution (BioRad)
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bioRxiv - Cell Biology 2024Quote: ... submerged with ECL solution (ECL Prime Kit; Amersham, UK) and chemiluminescence signals were detected using the ChemiDoc system from BioRad. The following antibodies were used ...