Labshake search
Citations for Bio-Rad :
501 - 522 of 522 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... The assay was performed in a 96-well plate in a final volume of 200 µL containing the sample and Bradford’s solution (BioRad, Marnes-la-Coquette, France). After a 5-min incubation at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... each lysate was diluted at a 1:1 ratio with a solution of 2× Laemmli sample buffer (#1610737EDU, Bio-Rad, Hercules, CA, USA), containing 5% (v/v ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the total lysates (20-30 µg/lane) were added with a solution to reduce the disulfide bridges (Reducing Agent 20X; Bio-Rad, Milan, Italy) and Laemmli Sample Buffer 4X (Bio-Rad ...
-
bioRxiv - Plant Biology 2024Quote: ... the membrane was washed twice with the 1x TBS-T buffer and then incubated with the Clarity Western ECL Substrate solution (Bio-Rad, California, USA). Signal detection of the membranes was performed by the Chemi-Doc Imaging system (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... and the protein bands were visualized with an enhanced chemiluminescence (ECL) solution (Clarity Western ECL Substrate, 1705062 Bio-Rad Labs, Des Plaines, IL). Quantitative analysis of the bands was performed with ImageJ software (version 1.49 ...
-
bioRxiv - Neuroscience 2024Quote: ... The transfer was carried out to 0.45 μm nitrocellulose membranes for 20 min per membrane at constant 25 mA in a semi-dry transfer chamber Trans-Blot® Turbo™ Transfer Solution from Bio-Rad (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2024Quote: ... Mice were anesthetized and transcardially perfused with an ice-cold PBS/heparin (50 u/ml) solution (Bio-Rad, Cat #1610780; Sigma, Cat# H3393), followed by 10% formalin (Polyscience ...
-
bioRxiv - Developmental Biology 2024Quote: ... A chemiluminescent signal was obtained from the blots using (ECL Prime Luminor Enhancer Solution, Cytiva, Canada) and the blot was developed using a ChemiDoc System (Bio-Rad Laboratories, Canada). Densitometries were calculated using ImageJ (Schneider et al. ...
-
bioRxiv - Bioengineering 2024Quote: ... Membranes were washed 3 more times before imaging with a 1:1 (v/v) ratio of Clarity Western enhanced chemiluminescence and peroxide solutions (Bio-Rad, Cat # 1705061). Primary and secondary antibodies used include ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μl of the resulting solution was then applied to either 4-12% Criterion™ XT Bis-Tris Protein Gels (BioRad, Hercules, CA, USA) or NuPAGE™ 4 – 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
-
bioRxiv - Genetics 2020Quote: ... tissue samples were incubated with 20 μl of base solution (25 mM KOH, 0.2 mM EDTA) at 95 °C for 30 min in a thermal cycler (BIORAD C100, BIORAD, Hercules, CA, USA), then 20 μl of neutralization buffer (40 mM TrisHCl ...
-
bioRxiv - Developmental Biology 2023Quote: ... The membranes were washed with TBST three times and then incubated with a secondary antibody for 1 h before using Luminol/enhancer solution and ClarityTM Western ECL Substrate (Bio-Rad Laboratories, Inc. US). Western blot images were scanned by using Gel Doc XR system (Bio-Rad Laboratories ...
-
bioRxiv - Biophysics 2024Quote: ... heated to 37°C for 10 min and resolved by SDS-PAGE in 10% (for Cx43 detection) and 12% (for AQP4 and GFAP detection) gels prepared with Mini-PROTEAN TGX Stain-Free Precast polyacrylamide solutions (Bio-Rad, Hercules, California, USA). gels were then activated for 5 minutes on a Chemidoc imaging system (Bio-Rad ...
-
bioRxiv - Cancer Biology 2020Quote: ... equal protein concentrations were loaded onto and separated in 12% (wt/vol) sodium dodecyl sulfate polyacrylamide gel (40% acrylamide/bisacrylamide solution; Bio-Rad, Hercules, CA; 161 0148). Proteins were transferred from the gel to 0.45 m pore size μ nitrocellulose membrane (Maine Manufacturing ...
-
bioRxiv - Microbiology 2020Quote: ... Amount 40 µg of total protein was run in 10% polyacrylamide gel at the same electrophoresis condition and then the gel was stained in coomassie blue solution (Bio-Rad Laboratories, Berkeley, USA; #1610786).
-
bioRxiv - Biochemistry 2022Quote: 5 µL aliquots of the CRISPR complex solution were buffer exchanged into 100 mM ammonium acetate using Biospin P-6 gel columns (Bio-Rad Laboratories Inc., Hercules, CA) prior to native mass spectrometry ...
-
bioRxiv - Microbiology 2020Quote: The pure cultures were grown in TSAYE plates for 24 h and DNA was extracted using 2% Chelex solution (Chelex 100 resin, Bio-Rad laboratory, Hercules, California, USA). The colonies (2-3 mm ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was washed again with 1x PBS-T (0.075 %) 3 times 10 minutes each and incubated with 1:1 ECL chemiluminescence solution (Clarity Western ECL, #170-5061, Bio-Rad Laboratories, Hercules, CA, USA). Signal was detected using an Amersham AI600 imager (Supplementary Table 5).
-
bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 12 hours in a blocking solution of 6% goat serum in PBS and then for 48 hours in the primary anti-tubulin antibody (Bio-Rad, Catalog# MCA77G, RRID: AB_325003) diluted in blocking solution at a final dilution 1:50 in a refrigerator at 5ºC ...
-
bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
-
bioRxiv - Microbiology 2023Quote: ... Blocking of the membrane was carried out by incubating in blocking solution for 1 h at room temperature (5% Bio-Rad Blotting-Grade Blocker in TSB-T buffer). Membrane was then incubated with anti-PcdA polyclonal antibody diluted 1:15,000 in blocking solution for 2 h at room temperature.