Labshake search
Citations for Bio-Rad :
351 - 400 of 580 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: The previously obtained plasma (1 µl) and gut mucus (30 µl) were separated on a 4-15% Mini-PROTEAN TGX gel (BioRad) under non-reducing conditions ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged (3,000gs for 30 min) and supernatant was filtered and loaded onto an equilibrated HisTrap HP column (GE) using NGC (Biorad). The HisTrap was pre-equilibrated with 6M Gdn-HCl ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred from the gel to an ethanol-immersed PVDF membrane in transfer buffer (30 min, 25 V, 1.0 A, Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were briefly washed in Tris-buffered saline with 0.1% Tween20 (TBS-T) ...
-
bioRxiv - Molecular Biology 2023Quote: ... plates were incubated for 4d at 30°C and photographed before washing using the ChemiDoc XRS+ molecular imager (Bio-Rad) under the blot/chemicoloric setting with no filter ...
-
bioRxiv - Physiology 2023Quote: ... Proteins were transferred from the gel to an ethanol-immersed PVDF membrane in transfer buffer (30 min, 25 V, 1.0 A, Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were briefly washed in Tris-buffered saline with 0.1% Tween20 (TBS-T) ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by 40 cycles of 95 °C for 5 sec and 60 °C for 30 sec using the CFX 96 real-time systems (Biorad). The 2−ΔΔCt method was used to analyze the data (Schmittgen and Livak ...
-
bioRxiv - Cancer Biology 2023Quote: ... A volume of 30 μL of resulting solution was run on an SDS-PAGE gel and imaged using the ChemiDoc MP System (BioRad). Imaging was followed by Coomassie Blue staining for total protein quantification.
-
bioRxiv - Molecular Biology 2023Quote: ... Lysate and dilutions were denatured in 1x Laemmli buffer at 95C for 30 seconds and resolved by SDS-PAGE on a 8-20% gradient gel (BioRad). Following trimming of the gel ...
-
bioRxiv - Pathology 2023Quote: ... Equal quantities of protein (30 - 60 μg) were resolved on TGX-precast gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subsequently transferred to PVDF blotting membranes (Millipore ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were pelleted by centrifugation at 500 rpm for 30 s and resuspended in 4x laemmli-sample buffer (Bio-Rad). Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... the gel was activated and then transferred for 30 min to a Trans-Blot Turbo Mini 0.2 µm nitrocellulose membrane (1704158, Bio-Rad) on a Trans-Blot Turbo transfer system (BioRad) ...
-
bioRxiv - Systems Biology 2023Quote: ... 40 cycles of PCR denaturing for 5 secs at 95 °C and annealing/extending for 30 secs at 55 °C) in real-time PCR system (CFX384 Touch, Biorad). The results were normalized to GAPDH from the same well ...
-
bioRxiv - Molecular Biology 2023Quote: ... 30 μg of total protein lysate was loaded for each sample onto Mini-PROTEAN® Precast Gels (Bio-Rad, 4568123) with 4 × Laemmli buffer (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... The mixture was allowed to stand at room temperature in the dark for 30 min before the detergent was removed by successive addition of BioBeads SM-2 (BioRad). Nanodiscs were concentrated using Vivaspin® 500 centrifugal filters (10 kDa MWCO PES membrane).
-
bioRxiv - Cell Biology 2023Quote: ... Gels were then separated from walls and bottom of the well and imaged every 30 mins for 1.5 hrs using a chemi-doc imaging platform (Bio-Rad). This first 1.5 hrs was considered the spontaneous contraction phase (SCP) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were separated by SDS-PAGE (30-50 μg total protein per lane) and transferred onto a 0.2 μm nitrocellulose membrane (Bio-Rad). Membranes were blocked with 5% skimmed milk for 30 min at RT and treated with primary antibodies (Supplementary Table S1 ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were denatured in Laemmli loading buffer as previously described,30 25 µg of protein was separated by 7.5% SDS-polyacrylamide acrylamide gel electrophoresis (SDS-PAGE) (Criterion, Bio-Rad) and blotted onto nitrocellulose membranes ...
-
bioRxiv - Biochemistry 2024Quote: ... Cell lysates (30 μg) were separated on precast 4-15% or 7.5% SDS-polyacrylamide Criterion gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were electrotransferred onto nitrocellulose membranes using the trans-blot turbo transfer system (constant voltage: 25V, maximum amperage: 1 A; 30 min, Biorad) and membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
-
bioRxiv - Microbiology 2024Quote: ... The samples were incubated for 30 min at room temperature and then resolved on a 7.5% TGX Stain free gel (Bio-Rad) at 120 V at 4°C for 1 hr ...
-
bioRxiv - Biochemistry 2024Quote: Unpolymerized 10% Tris-HCl gel solution (250 mL) was prepared by combining 30% bis/acrylamide (83.33 mL, #1610156, BIO-RAD), 1.5M Tris-HCl ...
-
bioRxiv - Genetics 2024Quote: ... and YMD4821-4826 were grown in 10-40mM caffeine for eight weeks in a 30°C shaking incubator (BioRad, 70rpm). Evolved clones YMD4666-4686 were isolated from populations grown at room temperature in 10mM caffeine for five weeks ...
-
bioRxiv - Microbiology 2024Quote: ... the samples were resolved on a 1.5% MOPS-buffered formaldehyde-denaturing agarose gel for 30 min at 80 V (PowerPacTM basic power supply, Bio-Rad) and visualized by gel imaging system (FluoroBox ...
-
bioRxiv - Microbiology 2024Quote: ... membrane for 30 min at 2.5 V and 25 mA using a semi-dry Trans-Blot Turbo system (Bio-Rad). Membranes were blocked by incubating them for two hours at room temperature (RT ...
-
Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... Elution of biotinylated protein was performed by heating the beads in 30 μL of 2X protein loading buffer (1610737; BioRad) supplemented with 2 mM biotin + 20 mM dithiothreitol (DTT ...
-
bioRxiv - Cancer Biology 2024Quote: Protein (30-60μg) were resolved via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast 4-20% gradient polyacrylamide gels (Bio-Rad). Blocked membranes were probed with primary antibody for 3 hours at room temperature and subsequently for 1 hour species-appropriate secondary antibody at room temperature using enhanced chemiluminescence substrate (Pierce) ...
-
bioRxiv - Biochemistry 2024Quote: ... It was first clarified by centrifugation at 10,000 g for 30 min and loaded onto a 10-mL self-assembled Affi-Gel Blue gravity column (Bio-Rad). The column was sequentially washed with 50 mL resuspension buffer containing 0.3 M NaCl ...
-
bioRxiv - Molecular Biology 2024Quote: ... 20% SDS and transferred using Transfer Buffer (Running Buffer, 20% Methanol) for 1 h and 30’ on Nitrocellulose membrane (BioRad). Membranes were blocked in 5 % milk ((W/V ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (0.2 μm, Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) in transfer buffer (50 mM Tris ...
-
bioRxiv - Molecular Biology 2024Quote: ... Fragmentation was stopped by addition of 80 μL 1 M Tris pH 6.8 and cleaned up twice with Micro Bio-Spin P-30 Gel Columns (BioRad, 7326223) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... The extracted RNA was then selected for 18-30 nucleotides on a 15% denaturing urea polyacrylamide gel (Bio-Rad, USA). RNA was eluted overnight in 0.3 M NaCl ...
-
bioRxiv - Cell Biology 2024Quote: ... They were transferred to nitrocellulose membranes at 25 V for 30 mins in the Trans-Blot Turbo system (Bio-Rad).
-
bioRxiv - Cancer Biology 2021Quote: ... The sample with fragmented RNA was neutralized with 1M Tris-HCl pH 6.8 and isolated by passing through P-30 column (Biorad, #732-6250). The NRO-reaction products containing biotinylated RNA was purified using Streptavidin C1 beads which were washed thrice with wash buffer (0.1N NaOH ...
-
bioRxiv - Developmental Biology 2021Quote: ... Equal amounts (30 μg) from each protein sample were separated by SDS-PAGE and transferred to PVDF membranes (PVDF; Biorad, USA). The membranes were blocked with 10% (W/V ...
-
bioRxiv - Evolutionary Biology 2020Quote: Library preparation for RNA extracted from NveAGO1 and NveAGO2 IP was size selected for 18-30 nucleotides on 15% denaturing urea polyacrylamide gel (Bio-Rad) followed by overnight RNA elution in 0.3M NaCl ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein lysates were diluted to obtain final sample concentration of 30 μg per sample and boiled in 1x Laemmli Sample buffer (Bio-Rad). Protein samples were loaded on pre-cast 4-20% TGX polyacrylamide gels (Criterion ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cleavage-related change in fluorescence signal of the probe was measured every 30 s for 60 min under incubation at 37°C using a Real-time PCR system (Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) or in transfer buffer (25 mM Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 μl of the cell suspension was spread on a single partitioned YPDA plate and incubated at 30°C for 48 h prior to imaging on a ChemiDoc imaging system (Bio-Rad). The area of all colonies ...
-
bioRxiv - Physiology 2020Quote: ... equal amounts (30 μg) of protein were resolved on 4–20% TGX stain-free gels (Bio-Rad Laboratories GmbH, Munich, Germany) and transferred onto PVDF membranes ...
-
bioRxiv - Genetics 2020Quote: ... a home-made gel filtration column (0,5 cm × 30 cm) was prepared using Bio-Gel agarose with a bead size of 75-150 μm (BioRad, 151-0440) and calibrated using a gel filtration standard (BioRad ...
-
bioRxiv - Biophysics 2021Quote: ... The mixture was diluted to ~30 mL with sample buffer and stirred at 4°C for 30 min prior removal of C12E8 by adding 4 g of Bio-Beads SM-2 (Bio-Rad) in stages of 0.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... 10% of the bead volume was transferred to a new tube and were boiled in 30 μL of 2X protein loading buffer (Biorad, 1610737) supplemented with 2 mM biotin and 20 mM dithiothreitol (DTT ...
-
bioRxiv - Neuroscience 2021Quote: ... Equal volumes (30 μg) of cell lysates from each condition were resolved by AnykD Mini-PROTEAN TGX precast gels (#456-9035, BIO-RAD) or 7.5% Mini-PROTEAN TGX precast gels (#456-8024 ...
-
bioRxiv - Neuroscience 2021Quote: ... and sonicated (twice; 70 % power for 30 sec, Sonopuls, Bandelin) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to the manufacturer’s instruction ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 72°C for 30 sec and followed by a melting curve) was performed in a CFX Real-Time Detection System (Bio-Rad) and relative expression calculated using the CFX Manager Software (Bio-Rad).
-
bioRxiv - Microbiology 2020Quote: ... 10 μl of the amplified product and 2 μl of 6X gel loading dye were added in the wells and the system was run at 50 V for 30 mins before visualization of the amplicons on the gel under a UV transilluminator (GelDoc, BioRad, Italy). The purified PCR products were then sequenced by Sanger dideoxy method using AB 3130 Genetic Analyzer (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... The plate was then incubated for 30 min at room temperature and the absorbance was measured at 595 nm using an iMark microplate reader (Bio-Rad). The H2O2 concentration was determined using a standard curve of known concentrations (up to 1 mM).
-
bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
-
bioRxiv - Genomics 2021Quote: ... 30-40 ng of RNA was used to make cDNA using the iScript Adv cDNA Syn Kit (Bio-Rad life Sciences). The RNA was diluted 1:50 in water for ddPCR analysis ...