Labshake search
Citations for Bio-Rad :
551 - 580 of 580 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: Westerns were performed by loading 30-50ug of total protein per well into 15 well polyacrylamide gels (Bio-Rad, California, USA, Cat #456-1086) and run for 30min at 200V ...
-
bioRxiv - Biochemistry 2022Quote: ... the HRP signals were visualized by using Chemi-Lumi One Super (02230-30, Nacalai Tesque) and a ChemiDocTM Imaging System (Bio-Rad Laboratories, Hercules, CA). The primary antibodies used in this study were anti-β-actin antibody (M177-3 ...
-
bioRxiv - Neuroscience 2021Quote: ... to a final concentration of 10 mM and shielded from light for 30 minutes.We performed an automated detergent compatible protein assay (DCA) (Bio-Rad, Hercules, CA, Cat. #5000111), normalization (to 1 μg/μL ...
-
bioRxiv - Microbiology 2021Quote: ... and O’nyong yong virus (ONNV) were subjected to a thermal gradient treatment from 30 to 60 °C for 3 h with a thermocycler (Bio-Rad T100 Thermal Cycler), after which samples were immediately titrated on Vero cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was diluted 1:30-1:50 in water and qPCR was performed using the iTaq Universal SYBR Green kit (Bio-Rad cat. no. 1725124). Each gene-specific primer and sample mix was run in triple replicates ...
-
bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
-
bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ddPCR was performed following a published protocol for examining yeast DNA replication [30] using Evagreen based chemistry (Droplet oil and Supermix; BioRad #1864005 and #1864033, respectively). ddPCR was performed on the indicated origins or non-origin control loci ...
-
bioRxiv - Genetics 2020Quote: ... tissue samples were incubated with 20 μl of base solution (25 mM KOH, 0.2 mM EDTA) at 95 °C for 30 min in a thermal cycler (BIORAD C100, BIORAD, Hercules, CA, USA), then 20 μl of neutralization buffer (40 mM TrisHCl ...
-
bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
-
LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
-
bioRxiv - Immunology 2024Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–20% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples containing 30 μg of total protein were separated by SDS-PAGE for 30 min at 270 V using 4–20% gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Proteins were transferred to nitrocellulose membranes ...
-
bioRxiv - Biochemistry 2023Quote: ... 70 μl of the resulting reaction mixture was first passed through a Micro Bio-Spin Bio-Gel P-30 spin column (Bio-Rad; 40 kDa MWCO) equilibrated with 20 mM HEPES pH 7.9 ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein lysate (30 μg) with 2X Laemmli buffer (in 1:1 ratio) was loaded onto a 4%–20% gradient gel (Bio-Rad, Mississauga, ON, Canada). The gel was transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Biochemistry 2023Quote: ... DsbAΔSP was oxidized with a 10-fold molar excess of AT-2 and roGFP2 was reduced with a 10-fold molar excess of DTT for 10 min at 20 °C and then the oxidant or reductant were removed using Micro Bio-Spin™ P-30 gel columns (BioRad, Feldkirchen, Germany, #7326223) or NAP™-5 columns (Cytiva ...
-
bioRxiv - Bioengineering 2024Quote: ... at a primer annealing temperature of 68°C for 30 cycles using a Bio-Rad C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, USA). The reverse and forward primer sequences were provided by Chen et al.[14] and procured from Integrated DNA Technologies (Coralville ...
-
bioRxiv - Cancer Biology 2024Quote: ... A total of 30-40 μg of proteins from the lysates were fractionated on SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad Laboratories, CA, USA). Membranes were probed with rabbit anti-HIF2α (ab243861 ...
-
bioRxiv - Physiology 2020Quote: ... and an equal amount of total protein (30 μg) per sample was subjected to standard SDS-PAGE using Mini-PROTEAN® TGX™ 4-20% 12-wells gels (Bio-Rad, Hercules, CA). Gels were electroblotted onto PVDF membranes (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... The enzymes were subsequently incubated at temperatures from 30 °C to 54 °C for one hour using a PCR thermocycler machine (Bio-Rad Laboratories, Hercules, CA, USA), followed by cooling for 10 minutes at 4 °C ...
-
Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... the beads were then washed five times with fresh binding buffer before being extracted with 30 µL of 2X Laemmli Buffer (Bio-Rad Laboratories, Hercules, CA, USA). 10 μL of the samples were separated via SDS-PAGE and stained with SimplyBlue safe stain while 1 μL of the samples were separated via SDS-PAGE ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads were then washed five times with fresh binding buffer before being extracted with 30 µL of 2X Laemmli Buffer (Bio-Rad Laboratories, Hercules, CA, USA). For Figure 5 E ...
-
bioRxiv - Plant Biology 2022Quote: ... The transfer “sandwich” was exposed to 25 V and 1 A for 30 mins by a Trans-Blot TURBO transfer system (Bio-Rad, catalog no. 170-4155). The membrane was incubated in 4.0% dry milk in PBS buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 to 30 µg of total protein was separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084) followed by an overnight transfer and western blot analysis.
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification conditions were initial denaturation at 95°C for 10 min followed by 40 cycles of 95°C for 10 s and annealing at 58-65 °C for 30 s (Bio-Rad CFX Connect Real-Time PCR) followed by melt peak analysis ...
-
bioRxiv - Systems Biology 2021Quote: ... plates were centrifuged for 10 min at 2000g and heated at 95C for 30 min (cell culture) or 60 min (tissue) in a thermal cycler (Biorad S1000 with 384-well reaction module) at a constant lid temperature of 110 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... The proteins (20-30 μg) of the tissue protein samples were subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad mini-PROTEAN TGX Gels, # 4561044) and transferred to polyvinylidene fluoride (PVDF ...
-
bioRxiv - Microbiology 2023Quote: ... TCA precipitated protein mixtures were dissolved in 30 µL 1 x Laemmli sample buffer and loaded on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories, Germany). After short 3 cm SDS PAGE separation ...
-
bioRxiv - Neuroscience 2024Quote: ... The supernatants were then transferred to a new tube and heated for 30 minutes at 37°C in 4X SDS-sample buffer (BIO-RAD, 355 mM β-ME added). Samples were then subject to SDS-PAGE and protein was transferred to a PVDF membrane ...
-
bioRxiv - Plant Biology 2021Quote: ... Leaf disks were ground to a fine powder evenly with a grinding tool and 120 μl extraction buffer added (30 μl Laemmeli sample buffer 4x [Bio-Rad], 24 μl DTT 1 M, 66 μl dH2O). Tubes were vigorously vortexed for 90 seconds ...