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Citations for Bio-Rad :
3901 - 3950 of 7697 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Cell Biology 2021Quote: ... The cell lysates were separated by electrophoresis on pre-cast 4-15% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PGE on a 4-15% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-Rad #456-8084) and transferred with the Trans-Blot® Turbo™ RTA Transfer Kit (Bio-Rad #170-4275 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated to 95°C for 5 min and then analyzed on a 4-12% gel (Bio-Rad Laboratories, Hercules, CA) using SDS running buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
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bioRxiv - Cancer Biology 2021Quote: Fifteen µg of protein per sample was separated on a BioRad Mini-protein 4-15% precast 12 well 20 µl gels (4561085, Bio-Rad, CA) and electrophoretically transferred to Odyssey Nitrocellulose membrane (P/N 926-31092 ...
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bioRxiv - Microbiology 2020Quote: ... or lacking (non-reducing) 100 mM β-mercaptoethanol and resolved by SDS-PAGE in a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD ...
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bioRxiv - Molecular Biology 2021Quote: ... even protein amounts were loaded and separated on mini-PROTEAN TGX precast 4-15% Bis-Tris gels (#4568085, Bio-Rad, Copenhagen, Denmark). Using a Bio- Rad Trans-Blot semidry transfer system proteins were transferred to a nitrocellulose membrane (#1704159 ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Immunology 2021Quote: ... Parasite lysate was reduced with β-mercaptoethanol (BME) and separated via SDS-PAGE in 4-20% Mini-PROTEAN TGX pre-cast gels (cat # 4561096, Bio-Rad) before transfer to PVDF membrane using a Trans-Blot Turbo Mini PVDF Transfer Pack (cat # 1704156 ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was quenched by incubation with 1 mM TCEP for 20 minutes at 37°C and 60% of the eluate was run on an SDS-PAGE gel (Mini-Protean TGX, 4-15%, Bio-Rad #4561084). The gel was stained with colloidal Coomassie (Bio-Rad #1610803 ...
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bioRxiv - Molecular Biology 2022Quote: ... The thawed samples were separated by SDS-PAGE using Mini-PROTEAN® TGX Stain-Free™ gels (usually 4-20%, Bio-Rad), and subsequently transferred to nitrocellulose membranes by a semi-dry blotting procedure as per manufacturer guidelines ...
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bioRxiv - Cancer Biology 2022Quote: ... An equal amount of protein from control and each experiment was loaded onto 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Gels, Bio-Rad, USA) and transferred to the nitrocellulose membranes (Amersham™ Protran® 0.45 μM ...
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bioRxiv - Cell Biology 2022Quote: ... Samples and PageRulerTM Prestained Protein Ladder (ThermoScientific) were loaded onto Mini-PROTEAN® precast gradient (4-20 %) polyacrylamide gels (Bio-Rad Laboratories) and proteins were separated in an electric field of 90-120 V for varying time spans in SDS Running Buffer (25 mM Tris-base ...
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bioRxiv - Immunology 2022Quote: ... and equal amounts of protein were loaded into a 4-15% gradient polyacrylamide gel (BioRad TGX™ Precast Midi Protein Gel #5671085) and proteins separated based on molecular weight using electrophoresis at 110V ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μg protein of whole cell lysate or variable volume of Flag-IP samples was loaded into 4-20% Mini-PROTEAN® TGX Stain-Free(tm) Gels (Bio-Rad) and then transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Equal volumes of sample were separated on 4–12% or 10% Criterion XT Bis-Tris Precast gels (Bio-Rad, Hercules, CA, USA) using XT MES Running buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein extract (20–30 μg) were resolved by SDS-PAGE (4–15% gradient precast TGX polyacrylamide gel, Bio-Rad Laboratories), followed by standard western blot procedure ...
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bioRxiv - Neuroscience 2022Quote: ... ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Bioengineering 2022Quote: ... were separated on a 4-10% SDS-polyacrylamide gel at 120 V for two hours using a PowerPac Basic setup (BioRad Laboratories Inc.). The proteins were transferred onto a 0.45 µm nitrocellulose membrane at 75 V for 90 min using a transfer assembly (BioRad Laboratories Inc.) ...
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bioRxiv - Cell Biology 2022Quote: The GST-FERRY interactor screen was performed at 4 °C in gravity flow filter columns (Poly- Prep® Chromatography Column, Bio-Rad). 500 μl Glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of 10-fold diluted cDNA was combined with 5 µL of iQ SYBR Green Supermix (Bio-Rad, Cat# 170-8880) and 0.5 µL of each 10 µM forward and reverse primers ...
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bioRxiv - Neuroscience 2024Quote: ... were resolved by SDS-PAGE on 4-15% Mini-PROTEAN TGX Stain Free gels (30 μL, 10 well comb, BioRad, Cat# 4568083) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were then separated by 6.5% (w/v) native polyacrylamide gels at 4 °C and the biotin-labeled probes was captured with ChemiDoc™XRS+ (Bio-Rad). The oligonucleotide probes used in this study are listed in Supplemental Data Set S1.
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bioRxiv - Plant Biology 2024Quote: Protein samples of whole cell extracts (5 μg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, US) and blotted onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 7.5% polyacrylamide gels or 4-20% gradient gels (Cat # 4561094 Bio-Rad, Hercules, USA). The gel was then transferred to a nitrocellulose membrane with a pore size of 0.45 µm (Cat # 1620115 ...
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bioRxiv - Physiology 2024Quote: ... were used for real-time PCR with gene-specific primers (Supplementary Table 4) and Tbp as a housekeeping gene on a CFX96™ Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Complete antibody conjugation was validated by running both conjugated and un-conjugated paired antibodies on a 4-15% SDS-PAGE gel (Bio-Rad #4561083) under non-reducing conditions ...
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bioRxiv - Microbiology 2024Quote: ... elution fractions with the highest protein concentrations were pooled and checked by SDS-PAGE (Laemmli, 1970) using a 4–20% Mini-PROTEAN gradient gel (BioRad, Munich, Germany). Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by adding 20 μg of protein lysate to 4-20% gradient Mini Protean TGX Stain Free Pre-Cast Gels (Bio-Rad Laboratories). Proteins were then transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: 40 μg of protein lysate was resolved on NuPAGE Novex 4-12 % Bis-Tris gels and transferred onto nitrocellulose membranes (Bio-Rad system). Membranes were blocked with 5 % BSA in TBS-T (10 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Lysates were separated by NuPAGE 4- 12 % Bis-Tris Gels (Thermo Fischer Scientific) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer Systems (Bio-Rad Laboratories). Membranes were blocked with Odyssey Blocking Buffer (Li-Cor ...
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bioRxiv - Biochemistry 2023Quote: ... Boiled lysates were loaded on a 4-15% Tris-glycine gradient gel and run at 150 V using Tris/Glycine/SDS buffer (Bio-Rad, 1610732) until loading buffer reached the bottom of the gel ...
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bioRxiv - Biochemistry 2023Quote: ... Histone samples (4 μg protein/well) or whole cell lysates (see above, 40 μg protein/well) were separated on precast gels (4-20%, BioRad, CA, USA) and transferred to 0.2 μm nitrocellulose membranes (Turbo transfer pack ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was performed on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories AG, Cat#4561094/4561096). Proteins were transferred to Trans-Blot Turbo 0.2 µm Nitrocellulose Transfer Membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The presence of the complex was assessed by 4-15% gradient SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein gels (BIORAD). Concentration of the RNAP in these fractions was determined by absorbance at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermofisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Ten microliters of each soluble or chromatin-bound nuclear fraction were loaded on 4-20% CRITERION TGX SDS-PAGE gels (Bio-Rad, #4561085).
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bioRxiv - Cell Biology 2024Quote: ... and 10 µg were then loaded and separated on precast 4–20% polyacrylamide Mini-PROTEAN TGX gels (Bio-Rad Laboratories, Hercules, CA). The proteins were then transferred to PVDF membranes (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of cell lysates were measured using BCA assay (Thermo 23225) and equal amounts of proteins were loaded onto 4 – 15 % polyacrylamide gradient gels (Bio-Rad 4561084) and fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Equal amounts (10 μL) of the eluted solution from the streptavidin pulldown were loaded in a 4-20% polyacrylamide gel (Bio-RAD 4561096) and separated with SDS-PAGE at 100 V for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... A normalized volume equivalent to 20 μg total protein in the predigested sample was resolved on a 4-12% Criterion XT Bis-Tris gel (Bio-Rad 3450124) at 100V for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... A total of 30 µg of protein per well was loaded onto a 4-15% precast SDS-PAGE gel (Bio-Rad, 4561083). SDS-PAGE running conditions are as follows ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 90 °C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were fractionated by SDS-polyacrylamide gel electrophoresis using a 4%–20% Mini-PRO TEAN TGX Gel (BioRad, Hercules CA, USA), and the resolved proteins were transferred by electroblotting onto polyvinylidene difluoride membranes (Immobilon-P PVDF Transfer Membranes) ...
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bioRxiv - Neuroscience 2022Quote: ... extracted proteins were first immobilized and resolved based on molecular weight on 4–15% Mini-PROTEAN TGX Precast gels (Bio-Rad Laboratories) through sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein from whole cell lysates and conditioned media were analyzed by running samples on SDS/polyacrylamide gel electrophoresis (4-15% Stain-free SDS-PAGE minigels) and transferred to PVDF membrane (Immun-Blot PVDF, Bio-Rad 1620177). Membranes were blocked in 3% BSA in Tris-buffered saline containing 1% Tween-20 (pH7.4) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples are prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 70 °C for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... were loaded onto 4%–12% sodium dodecyl sulfate–polyacrylamide gels (Absin, China) for 40 min using an electrophoresis apparatus (Bio-Rad, USA) and transferred to polyvinylidene difluoride membranes (Millipore ...