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Citations for Bio-Rad :
3651 - 3700 of 7321 citations for 4S 6 8 difluoro 3 4 dihydro 2H 1 benzopyran 4 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... The extracts were separated on 6% polyacrylamide (Biorad) gel and transferred onto nitrocellulose membranes in Tris/glycine transfer buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mL gravity flow columns were from Biorad, Germany.
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bioRxiv - Systems Biology 2024Quote: ... or QuantStudio 6 Flex systems (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were generated by sonicating and boiling 1*10^6 cells in 100ul of 2X Laemmli Buffer (Bio-Rad) with BME ...
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bioRxiv - Genetics 2024Quote: ... was added to the reactions and the products were separated on 6% polyacrylamide gels (ratio acrylamide:bisacrylamide 19:1, Bio-Rad) in TAE (40mM Tris ...
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bioRxiv - Cell Biology 2020Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels and the Trans-Blot® Turbo™ transfer system (Bio-Rad). The following antibodies ...
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bioRxiv - Cell Biology 2020Quote: Equal amounts of protein were loaded onto 12% (v/v) resolving SDS-PAGE gels with 4% (v/v) polyacrylamide stacking gels using Precision Plus Protein™ dual colour standards (Bio-Rad, Hercules, CA, USA). The gels were run in a Mini-Protean® Tetra Cell system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were eluted in SDS-PAGE sample loading buffer and separated in 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad# 4561094, 4561096) either for western blot (WB ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates were prepared using RIPA buffer (see lysis and click chemistry methods below) and run on SDS Tris-Glycine gels (Precast 4 - 20% Bio-Rad, or hand-casted 20%). Gels were transferred to low-background PVDF membranes using the BioRad TransBlot Turbo system and blocked with 5% milk powder in TBS + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2020Quote: Twenty-fold concentrated viral stocks or purified rgp120 were separated by SDS-PAGE under reducing conditions using a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc. Hercules, CA), and then transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified at 13,200 rpm for 15 min at 4°C prior to quantification by Lowry assay (Bio-Rad cat # 5000113 and cat # 5000114). Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Physiology 2020Quote: ... and an equal amount of total protein (30 μg) per sample was subjected to standard SDS-PAGE using Mini-PROTEAN® TGX™ 4-20% 12-wells gels (Bio-Rad, Hercules, CA). Gels were electroblotted onto PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE (4-15% acrylamide, 150V) and transferred to PVDF membranes using the Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated for 1 hr in 5% milk (in TBS-T ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed using the primer pairs listed in Table 4 and iTaq Universal SYBR Green Master Mix on a CFX384™ Real-Time PCR detection system (Bio-Rad Laboratories, Hercules, CA). Fold changes in gene expression were calculated by the ΔΔCt method using the weighted average of Ct values for the housekeeping genes Pol2ra ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2022Quote: For each lane, ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of proteins were analyzed in a polyacrylamide gel (4–20% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Marnes-la-Coquette, France) and separated by electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels, Bio-Rad Laboratories, CA) and transferred onto PVDF membranes (Amersham Hybond ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4-20% gradient gels (Bio-Rad #4561095; Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µL of media was reduced and denatured prior to gel electrophoresis on a 4-15% tris-glycine gel (#4561084, Bio-Rad Laboratories, Hercules, CA, USA) for 2 hours @ 175 mV ...
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bioRxiv - Biochemistry 2024Quote: ... 20 to 30 µg of total protein was separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084) followed by an overnight transfer and western blot analysis.
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...
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bioRxiv - Neuroscience 2024Quote: ... were separated under reducing conditions by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (4-20% Criterion TGX Stain-free gels, Bio-Rad Laboratories, Hercules, CA, USA) and transferred to Polyvinylidene fluoride (PVDF ...
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bioRxiv - Genetics 2024Quote: ... 30μg total protein from tissue samples with 4X Laemmli buffer was heat denatured at 99°C for 5 minutes and ran on resolving SDS PAGE gel (Biorad 4-20% precast gel #4561096). Sperm protein samples were prepared using a low volume of lysis buffer (∼100) ...
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of protein were subjected to 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (cat. no. 1620112; Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked using Intercept® (TBS ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of ion exchange-purified phage was added to 4 µl of cells and after 5 min of incubation mixed 1:2 (v/v) with 6 nm BSA-gold tracers (Aurion) which were transferred to RCV by Micro Bio-Spin® 6 columns (Biorad). Four µl of the mixture were applied onto glow-discharged Quantifoil® R2/1 200 mesh copper grids ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 hours followed by 20 μM Nigericin for 3 hours (ICT9146, Bio-Rad).
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Immunology 2021Quote: ... Proteins were separated in 8% SDS-PAGE mini-gel (Bio-Rad), blotted to PVDF membrane (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... DNA molecular weight standard (BioRad, CHEF DNA size 8-48-kb) was used as reference.
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bioRxiv - Evolutionary Biology 2020Quote: ... then to 97°C for 8 min in a thermocycler (BioRad). We centrifuged the samples and froze the supernatant for further use.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were analyzed in quadruplicate on 8-16% TGX gels (BioRad) that had previously been equilibrated in 0.5X TBE for 30 minutes at 150V ...
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bioRxiv - Cell Biology 2021Quote: ... 8 μl of 10% (w/w) tetramethylethylenediamine (TEMED, BioRad 161-0800) accelerator and 4 μl of 10% (w/w ...
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bioRxiv - Cell Biology 2022Quote: ... Cell were labelled with rabbit anti-IL-8 antibody (Biorad Biosciences), mouse anti-IL-1β antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit anti-IL-8 antibody (Biorad Biosciences) and mouse anti-IL-1β antibody (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were clarified by a brief centrifugation (10,000 g for 5 min at 4°C) and the supernatants were collected and diluted with 2× reducing Laemmeli buffer (Bio-Rad, Hercules, CA, USA, cat. #1610737). For preparation of cell culture lysates ...
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bioRxiv - Immunology 2021Quote: Proteins (20 µg per well) were separated in 4–15% Criterion™ TGX™ Precast Midi Protein Gels with 26 wells (Bio-Rad, Hercules, CA, USA) at a constant voltage of 120 V ...
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bioRxiv - Microbiology 2020Quote: ... 8.4 μL of PCR Probe water and 10 μL of the 2x commercial reaction mixture (SYBR® Green Master Mix, Bio-Rad. A LightCycler96® (Roche) device was used and PCR conditions applied were ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min before loading into a 4 – 20% Criterion TGX precast Midi protein gel (Bio-Rad, Hercules, CA, USA; Cat# 5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...