Labshake search
Citations for Bio-Rad :
3601 - 3650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were then visualized on the ChemiDoc Imaging System (Bio-rad) following development with WesternBrightTM ECL or Sirius (Advansta ...
-
bioRxiv - Cancer Biology 2024Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA Synthesis Kit (Bio-Rad). A 1/10 dilution of the cDNA was then used for qRT-PCR analysis utilizing the SYBR Green PCR Master Mix (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Conversion of one µg of RNA into cDNA was performed using the iScript cDNA Synthesis Kit (1708890; Bio-Rad Laboratories) and Master Cycler Pro Device (EPPE6324000.516 ...
-
bioRxiv - Cancer Biology 2024Quote: ... was determined by using the QuantaSoft Analysis Pro (Bio-Rad) software.
-
bioRxiv - Cancer Biology 2024Quote: ... and cDNA was synthesized from 1μg RNA using iSCRIPT as per the manufacturer’s instructions (Biorad, Hercules, CA). qPCR analysis of samples was performed on the CFX96 Real-Time System (Biorad ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
-
bioRxiv - Cancer Biology 2024Quote: ... with Sso Advanced Universal SYBRGreen supermix (Biorad) with the following thermal cycler conditions for all reactions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and blotted onto nitrocellulose membranes (Bio-Rad) following standard conditions ...
-
bioRxiv - Cancer Biology 2024Quote: ... The resulting complementary DNA (cDNA) was analyzed by quantitative PCR using iTaq Universal SYBR Green Supermix (Bio-Rad) in a ViiATM 7 Real-Time PCR System (Thermo-Fisher) ...
-
bioRxiv - Cancer Biology 2024Quote: ... equipped with a 5-mL Foresight Nuvia HPQ anion-exchange column prepacked with CHT ceramic hydroxyapatite XT mixed-mode chromatography media (Bio-Rad) or 25-mL column in-house packed with the same CHT media ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using a Trypan Blue exclusion assay or TC20 automated cell counter (BioRad, Hercules, CA).
-
bioRxiv - Cancer Biology 2024Quote: ... The viable cell density (VCD) and viability were measured using TC20 automated cell counter (Bio-Rad, Hercules, CA) or hemocytometer and trypan blue (Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Ten microliters of each soluble or chromatin-bound nuclear fraction were loaded on 4-20% CRITERION TGX SDS-PAGE gels (Bio-Rad, #4561085).
-
bioRxiv - Cancer Biology 2024Quote: ... denatured and spotted on a nitrocellulose membrane using the Bio-Dot SF (Bio-Rad, Hercules, CA, USA). Then ...
-
bioRxiv - Cancer Biology 2024Quote: ... using the transblot Turbo Transfer System (BioRad, 1704150). The membranes were blocked for 1 h with 5 % non-fat dry milk (Cell Signaling Technology ...
-
bioRxiv - Cancer Biology 2024Quote: ... in a BioRad IQ5 detection system (Bio-Rad, Portland, ME). The transcript level of the CoChR gene was normalized to the average levels of Gapdh and Rpl32 ...
-
bioRxiv - Cancer Biology 2024Quote: ... SERPINH1 (Biorad #VMA00970), phospho-ERK1/2 (Cell Signaling Tech pThr202/pTyr204 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Laemmli Buffer (BioRad) and 10% β-mercaptoethanol were added to the samples and boiled for 5 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... resuspended in medium and counted at different incubation times (0, 24, 48, 72 and 96 h) on a TC 20 automated cell counter (Biorad). The experiments were conducted in triplicate for each timepoint ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells were centrifuged for 15 min at 15,000 x g at 4°C and the supernatant was quantified with Quick Start Bradford 1X Dye Reagent (BioRad), using an albumin curve to determine the concentration of protein to be placed ...
-
bioRxiv - Cancer Biology 2024Quote: ... and equal protein quantities were then loaded on 4-20% polyacrylamide gels (Biorad) or 4-20% Tris-Glycine gels (Introvigen ...
-
bioRxiv - Cancer Biology 2024Quote: ... and Western blot analysis was performed using the Anti-TSHR rabbit IgG antibody (Biorad, Hertfordshire, UK). The secondary anti-IgG rabbit antibody and the chemiluminescence system SignalFire™ ECL were from Cell Signaling Technology (Danvers ...
-
bioRxiv - Cancer Biology 2024Quote: ... Intensities were measured using the ImageLab software (Bio-Rad).
-
bioRxiv - Cancer Biology 2024Quote: ... Acquisitions were performed using the ChemiDoc Imaging System (Bio-Rad). Intensities were measured using the ImageLab software (Bio-Rad).
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were then denaturated in Laemmli buffer (Bio-Rad) at 95°C for 5 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using the SYBR Green SuperMix (Quanta) using a C1000 Real-Time System (Bio-Rad). Data were normalized using the r18S gene as endogenous control and fold change was calculated using the comparative Ct method (-ddCt) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was measured using the Bradford assay (Bio-Rad Laboratories). An appropriate volume of Laemmli sample buffer was added to equal amounts of total protein followed by boiling for 5 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... and ChemiDoc imaging system (Bio-Rad Laboratories, USA).
-
bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of RNA to cDNA was performed using the iScript cDNA synthesis kit (Bio-rad Laboratories, USA). cDNA was mixed with SYBR Select Master Mix for CFX (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were subjected to SDS–PAGE electrophoresis before transfer to nitrocellulose membrane (Bio-Rad Laboratories, USA). After blocking for 1 h in 5% skim milk/PBST ...
-
bioRxiv - Cancer Biology 2024Quote: ... and transferred onto nitrocellulose membrane (BioRad 0.2μm) for 3h at 250mA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration in the clarified supernatant was quantified using Bradford Protein Assay Reagent (Bio-Rad Laboratories). 40μg of protein lysate was loaded onto SDS-PAGE gel using a Mini-Protean System (BioRad ...
-
bioRxiv - Cancer Biology 2024Quote: ... in chemiDoc imaging system (Bio-Rad Laboratories).
-
bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was quenched with final concentration of 125 mM Glycine (Bio-Rad). Cells were collected and incubated in 7 mL of Cell Lysis Buffer (5 mM Tris [pH 8] ...
-
bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was quenched with final concentration of 125 mM Glycine (Bio-Rad). Cells were fractionated as described by Neugebauer’s lab (Brugiolo et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitants were then gently washed three times with KPBS and eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis.
-
bioRxiv - Cell Biology 2024Quote: ... Protein band intensities were quantified using ChemiDoc MP Imaging System and Image Lab software (Bio-Rad, Hercules, CA, USA).
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (20-30 μg) were resolved by 8 to 15% SDS PAGE MiniPROTEAN® TGX gels (Bio-Rad), from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad ...
-
bioRxiv - Cell Biology 2024Quote: ... and heated to 98°C for 5 min and transferred to Trans-Blot PVDF Transfer membranes (Bio-Rad, #1704157). Membranes were blocked for 1 h at room temperature with TBS-T (0.1% Tween 20 ...
-
bioRxiv - Cell Biology 2024Quote: ... the protein bands were visualized using ClarityTM Western Blot ECL (Bio-Rad) and recorded with ImageQuant LAS4000 (Fujifilm).
-
bioRxiv - Cell Biology 2024Quote: ... from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad) supplemented with 5% of β-mercaptoethanol (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: ... The gel in Figure 7B was run on a 12% Mini-Protean TGX precast protein gel (Bio-Rad, #4561034), whereas the one in Figure 7C was run using hand-cast gels ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were incubated at room temperature with end-over-end mixing for 30 min before adding washed BioBeads SM2 (Bio-Rad 152-39120) in two stages ...
-
bioRxiv - Cell Biology 2024Quote: ... pH 6.8) or 230 μL 2x Laemmli Sample Buffer (Bio-Rad, #1610737), and samples were immediately denatured for 5 minutes at 95 °C.
-
bioRxiv - Cell Biology 2024Quote: Cells were lysed in 2x Laemmli buffer (Bio-Rad) containing 100 mM DTT and heated for 10 min at 95°C ...
-
bioRxiv - Cell Biology 2024Quote: ... and total RNA was extracted by Aurum Total RNA Mini Kit (Bio-Rad,Hercules, California, USA).) ...
-
bioRxiv - Cell Biology 2024Quote: ... eluate was incubated at 70 °C then run on 4-12% NuPAGE gell and transferred to LF PVDF membrane (BioRad). Western-blot analysis were done with the next antibodies ...
-
bioRxiv - Cell Biology 2024Quote: ... and total RNA was extracted by Aurum Total RNA Mini Kit (Bio-Rad,Hercules, California, USA).Using SuperScript II reverse transcriptase random hexamer primer total RNA was reverse transcribed into cDNA ...
-
bioRxiv - Cell Biology 2024Quote: ... The signal was detected and visualized by ChemiDoc XRS+ (Biorad) or Alliance Q9 (UVITEC ...