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Citations for Bio-Rad :
3551 - 3600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Dharmacon, Cambridge, UK) or siRNAs targeting MYC (D-003282-14 & D-003282-35, Dharmacon) using siLentFect transfection reagent (1703362, Biorad, Hercules, CA, USA) (see Table S4 for siRNA sequences) ...
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bioRxiv - Cancer Biology 2024Quote: ... with the SYBR Green PCR Mix (Bio-Rad, USA). The primers for detection are listed below ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... These samples were denatured at 100 °C in 4 × XT Sample Buffer (Bio-Rad, USA). N-glycosylation modification of proteins was assessed using the PNGase F Glycan Cleavage Kit (A39245 ...
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bioRxiv - Cancer Biology 2024Quote: ... on a ChemiDoc imaging system from BioRad. All antibodies and their concentrations used in this study can be found in Supplementary table 6.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed for siRNA-mediated Brca1 and Brca2 depletion on qPCR CFX 96 Thermocycler (Bio-Rad) using gene-specific primers (Table EV 3) ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR reaction was analyzed on CFX opus Real-Time PCR device (Bio-Rad) and data were normalized to the expression levels of GAPDH as housekeeping gene.
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bioRxiv - Cancer Biology 2024Quote: ... and images were captured using a ChemiDoc XRS-System (Bio-Rad, USA). Signal intensities were normalized to GAPDH or Actin ...
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bioRxiv - Cancer Biology 2024Quote: ... protein was transferred to a PVDF membrane (Bio-Rad). Membranes were blocked in 5 % skim milk in TBST (50 mM Tris–HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... blots visualization was achieved using an enhanced chemiluminescence detection kit (Bio-Rad, USA) and images were captured using a ChemiDoc XRS-System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... First-strand complementary DNA synthesis was performed with using iScript reverse transcription super-mix from Bio-Rad. Real-time qPCR was performed using SYBR green on Applied Biosystems QuantStudio 5 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Cancer Biology 2024Quote: ... Optical density was measured at 450 nm using a microplate spectrophotometer (Bio-Rad). Experiments were performed in triplicate.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR was performed using manufacturers’ instructions for SSO advanced SYBR green kit (Biorad) on ABI-qPCR system using equal amount of template (50–100 ng cDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to PVDF membranes in 1X transfer buffer (Biorad) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: An equal number of cells from different treatments were suspended in Laemmli sample buffer (Bio-Rad, #1610747) containing 50mM DTT (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by brief rinse in TBS (Bio-rad, #1706435) and blocking with 5% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred to nitrocellulose membranes using transfer kit (Bio-rad, #1704156), followed by brief rinse in TBS (Bio-rad ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then rinsed in TBST and dipped in an ECL WB Detection Reagent (Bio-rad #1705061) prior to exposure ...
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bioRxiv - Cancer Biology 2024Quote: ... and electrophoresis was carried out in TG-SDS buffer (Bio-rad #1610732) for 1-1.5 hours at 120V ...
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bioRxiv - Cancer Biology 2024Quote: ... inflammatory cytokines and chemokines were measured using the mouse multiplexed Luminex assays (Bio-Rad, Hercules, CA) according to the manufacturer’s protocols in the University of North Carolina at Chapel Hill (UNC-CH ...
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bioRxiv - Cancer Biology 2024Quote: ... for quantitative real-time PCR on a CFX384 instrument (Bio-Rad). Conditions applied included 40 cycles of amplification ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to transfer proteins from gel to the LF-PVDF membrane (Bio-Rad, cat. no: 1704274). For immunoblotting ...
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bioRxiv - Cancer Biology 2024Quote: ... (Bio-Rad, 161-0772). Trans-Blot® Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... and bands were imaged on a BioRad ChemiDoc MP (BioRad,12003154). Quantification of bands was performed using the ImageLab 6.1 software (BioRad).
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bioRxiv - Cancer Biology 2024Quote: ... or fluorescent Starbright Blue700 secondary antibodies (Biorad,12004161) and bands were imaged on a BioRad ChemiDoc MP (BioRad,12003154) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantification of bands was performed using the ImageLab 6.1 software (BioRad).
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bioRxiv - Cancer Biology 2024Quote: ... and protein size was estimated using the Precision Plus All Blue (BioRad) ladder ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitated DNA was analyzed by qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad) using Primer 4 (Tang et al. ...
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bioRxiv - Cell Biology 2024Quote: ... reverse transcription was performed with iScript™ Reverse Transcription Supermix for RT-qPCR (Bio-Rad). qPCR was performed real-time PCR with SsoAdvanced Universal SYBR Green Supermix (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: ... qPCRs were performed in 10 μL in 96-well plates on a CFX OPUS-96 thermocycler (Bio-Rad) with 5 μL of 2X PerfeCTa SYBR Green Supermix (Quantabio) ...
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Complementary Cytoskeletal Feedback Loops Control Signal Transduction Excitability and Cell PolaritybioRxiv - Cell Biology 2024Quote: ... 800 μl of rolled cells were transferred to a 4-mm-gap electroporation cuvette (Bio-Rad, 1652081), using pipette tips whose edges were cut off to avoid breaking clusters ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Cell Biology 2024Quote: ... in CFX384 machine (Bio-Rad Laboratories). β-Actin expression was used for normalization ...
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bioRxiv - Cell Biology 2024Quote: ... The qRT-PCR was conducted using a CFX384 Real-Time PCR System (Bio-Rad, USA). The reaction mixture comprised of 5 μl SYBR Green (Biosharp ...
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bioRxiv - Cell Biology 2024Quote: ... and imaged using a multi-imager (Biorad) for colony counting.
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... and transferred to PVDF membranes using a Transblot Turbo system (BioRad). The membranes were incubated in blocking buffer (5% skim milk in TBS-Tween 0.05% ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis was then performed using reverse transcription kits (Bio-Rad Laboratories, Hercules, CA). qPCR was carried out with TaqMan® probe/primers (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... version 6.0.1 (Bio-Rad Laboratories).
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... samples were run on 4-15% gradient Mini-Protean gels (BioRad) and transferred to PVDF membranes using a Transblot Turbo system (BioRad) ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... before imaging on a ChemiDoc system (BioRad).
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bioRxiv - Cancer Biology 2024Quote: ... The resolved proteins were transferred to nitrocellulose using a wet transfer unit (Bio-Rad Laboratories AB, Solna, Sweden). The efficiency of immunoblotting and equal loading of proteins was verified by staining of the nitrocellulose membrane with 0.1% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad Laboratories AB, Solna, Sweden). Real-time PCR was done in triplicate using the qPCRBIO SyGreen 2×Master Mix (PCR Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then used for qPCR with SsoAdvanced Universal SYBR (Bio-Rad #1726275) using FGFR4 primers ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins were separated using Mini-PROTEAN TGX Stain-Free Pre-cast gels (BioRad, 4568093) at 100 V for 80-90 min in the BioRad Mini-Protean Tetra system and transferred to a nitrocellulose membrane using the BioRad Trans-Blot Turbo Transfer System ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred electrophoretically onto a PVDF 0.45 μm membrane (BioRad). Membranes were blocked for 1 hour at room temperature in blocking buffer consisting of 5% milk or 1 % BSA diluted in Tris buffer saline plus 0.1% Tween 20 (TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins and ladder were then imaged using the ChemiDoc MP imaging system (Bio-Rad Laboratories, Inc.) using the autoexposure and chemiluminescence to observe the bands and 635 nm of light with autoexposure for visualization of the ladder.
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts underwent electrophoresis under reducing conditions on 4–15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and then transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... the array membranes were scanned using ChemiDoc Imaging System (BioRad, 12003153). ImageJ was used to quantitate the dots on the array membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Analysis for gene expression by quantitative real-time PCR was performed using SsoAdvanced Universal SYBR Green Supermix (BioRad, 1725271).