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Citations for Bio-Rad :
3551 - 3600 of 4474 citations for Human Cathepsin B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Approximately 700 ng total purified RNA was used to generate cDNA with the iScript cDNA Synthesis kit (Bio-Rad). cDNA was used for PCR to quantify gene expression for SLC15A4 ...
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bioRxiv - Genomics 2023Quote: ... and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, #1708890). We used RT-qPCR to quantify MYC expression (forward primer ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted and purified from RPE tissue punches using Single Shot Cell Lysis Kit (Bio-Rad Laboratories). cDNA synthesis was performed from 1.5 μg RNA using RT² First Strand Kits (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: qRT-PCR was performed on a C1000 Thermal Cycler using SsoFast™ EvaGreen Supermix kit (Bio-Rad, Hercules, CA) according to the instructions ...
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bioRxiv - Immunology 2023Quote: ... The protein bands were visualized using an ECL kit (Meilunbio, CHN) and imaged on ChemiDoc XRS+ (Bio-Rad, USA).
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bioRxiv - Cancer Biology 2023Quote: Retrotranscription of RNA was performed using 3μg of total RNA in a 40μl final volume reaction mixture using iScript Advanced cDNA Synthesis Kit for RT-qPCR (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The total RNA (1.2 μg) was used to synthesize single-stranded cDNA using iScript cDNA synthesis Kit (Bio-Rad) and T100 Thermal Cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... 500ng RNA was used as input for cDNA synthesis through iScript™ advanced cDNA synthesis kit (Bio-Rad 1725038) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was revealed with the Clarity™ Western ECL Substrates Kit (Bio-Rad Laboratories Inc, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using the iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories, Hercules, CA, USA) with 2.0-20 ng input corneal RNA according to a manufacturer protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... total RNA samples were reverse transcripted into first-strand cDNA using the iScript Select cDNA Synthesis kit (Bio-rad). 1 µl of a 10X diluted cDNA sample was then used in the qPCR reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-rad) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Freshly isolated mRNA was reverse transcribed into complementary DNA with random primers by iScript cDNA Synthesis Kit (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription PCR was performed on the sample to generate cDNA using iScriptTM cDNA Synthesis Kit (BioRad, Hercules, USA) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA was then reverse transcribed using an iScript cDNA Synthesis Kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) following the manufacturers guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed using 350 ng (CD11B+) and 1,000 ng (brain) mRNA with an iScript cDNA synthesis kit (BioRad). Real-time quantitative PCR was performed on triplicate samples using SYBR Green Supermix (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Signal was detected using the HCL kit (ECLTM Primer Western Blotting Detection Reagent, AmershamTM) and a Chemidoc system (Biorad). Signal were quantified with the ImageLab Software.
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bioRxiv - Immunology 2023Quote: ... The cDNA obtained was subjected to qRT-PCR using the itaq™ Universal SYBR green kit (Bio-Rad, #1725122) and the CFX 96 Real-Time System (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... A 500-ng aliquot of the isolated RNA was converted to cDNA with the iScript Advanced Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 7µl nuclease-free water and 10 µl of SSo advanced universal SYBR Green qPCR kit (Bio-Rad, Ref: 1725272) for gill derived samples ...
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bioRxiv - Bioengineering 2024Quote: ... transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) at 25V ...
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bioRxiv - Molecular Biology 2024Quote: ... Five hundred nanograms of total RNA were used for a SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad). rRNA was depleted with Mouse/Rat riboPOOL (siTOOLs Biotech) ...
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bioRxiv - Molecular Biology 2024Quote: ... Four hundred nanograms of total RNA were used for a SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supernatant was recover and protein concentration was measured using the DC™ Protein Assay Kit II (Bio-Rad, 5000112). Lysates were boiled in the NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression was quantified using the iTaq Universal One-Step RT-qPCR Kit (cat. 1725150, BioRad, Hercules, CA, USA) in Applied Biosystems and normalized to GAPDH or Actb ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5 μg total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad; #1708891). Real-time quantitative polymerase chain reactions (RT-qPCR ...
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bioRxiv - Bioengineering 2024Quote: ... Sample concentrations were normalized to 1 ng/µL during cDNA preparation using the iScript Synthesis Kit (Bio-Rad, 1708841), following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1µg total RNA per sample using a Bio-Rad cDNA Synthesis Kit (Bio-Rad Laboratory). Quantitative real-time polymerase chain reaction (RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized from 500 ng RNA using the iScript™-cDNA Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time quantitative PCR was carried out using mRNA-specific primers (Table S1) ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was performed in triplicate with a PCR starter kit in CFX96 Real-Time System (Bio-Rad, USA). hsa-miR-16-5p was used as an internal control to normalize the gene expression ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes ...
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bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined by a detergent compatible DC™ Protein Assay Kit II (Bio-Rad Laboratories, #5000112). For Western blot analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg of total RNA was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Standard curves were run for all primer pairs to ensure high efficiencies (>97% ...
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bioRxiv - Plant Biology 2024Quote: The AtGCN5 coding sequence amplified from Arabidopsis Col-0 cDNA using iProof™ High-Fidelity PCR Kit (Bio-Rad) was inserted into pDONR221 vector (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were transferred to Nitrocellulose membrane with the Trans-Blot Turbo RTA transfer kit (BIO-RAD Cat.#170-4270). The chemiluminescent signal was detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... was used to determine RNA concentrations and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 200 ng of RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 µg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). FCGRT ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted using the Total RNA Kit I (EZNA) and cDNA synthesis was carried out using iScript (Biorad) according to the manufacturers instructions and using 300 ng total RNA for each sample.
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bioRxiv - Cell Biology 2020Quote: ... RNA (1µg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... We used SuperSignal West Pico or Dura chemiluminescent detection kits (Thermo Fischer) and the chemiluminescent signal was acquired using the ChemiDoc system equipped with an XRS camera (BioRad).
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bioRxiv - Cell Biology 2020Quote: ... 500ng of RNA was reversed transcribed into cDNA in a 20 μL reaction volume using iScript cDNA synthesis kit (Biorad) in accordance to manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... Quantity of libraries was assessed by performing digital droplet PCR (ddPCR) with Library Quantification Kit for Illumina TruSeq (BioRad, #1863040). Libraries were diluted to 2 nM and paired-end sequencing was performed on a HiSeq 2500 sequencer (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized using 600 ng input total RNA per 40 ¼l reaction with the iScript cDNA Synthesis Kit (BioRad), cycling for 5 min at 25°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was washed three times with TBST and detection was performed with the Clarity™ ECL kit (Bio-Rad) according to the manufacturer’s instructions and visualized with a CCD camera of the Odyssey Fc imaging system (Li-COR Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... was used to isolate the total RNA followed by cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, 1708891). Real-time qPCR quantification was done on a Bio-Rad CFX96 Real-Time system using SsoFast EvaGreen Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA was harvested as described above and used to create cDNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories). qPCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufactureŕs instruction and measured using the EonTM High Performance Microplate Spectrophotometer (BioTek ...