Labshake search
Citations for Bio-Rad :
3351 - 3400 of 4474 citations for Human Cathepsin B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... HMGA2 fibroid (HMGA2F) expression was determined by RT-qPCR using the iScript Advanced cDNA Synthesis Kit (BioRad, #1725037) for the reverse transcription and HMGA2 PrimePCR primer (BioRad ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The quantification of mRNA levels was conducted via an iTaq Universal SYBR Green One-Step Kit (Bio-Rad). The assay was performed on an ABI StepOnePlus PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... We assessed library quantities using digital droplet PCR (ddPCR) with the Library Quantification Kit for Illumina TruSeq (BioRad). We diluted libraries to 2nM and performed paired-end sequencing using a HiSeq 2500 (Illumina).
-
bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
-
bioRxiv - Genomics 2024Quote: ... chr10:28,469,205-28,662,312 in EquCab3.0) were extracted from 10 ml bacterial cultures with the Quantum Prep Plasmid miniprep kit (BioRad), according to supplier instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... and transferred to ethanol-activated 0.2μm PVDF membranes using the Trans-Blot Turbo RTA Mini Transfer kit (#1704272) from BioRad. Each blot was blocked using 5%/0.1% BSA/Tween in TBS and incubated with primary antibody overnight at 4°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA was generated using 1000 ng of total purified RNA following iScript cDNA synthesis kit (1708890, Bio-Rad) manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: RNA was extracted using the OMEGA total RNA kit and reverse transcribed using iScript reverse transcriptase (Bio-Rad). qPCR was performed using TB Green Premix Ex Taq (Takara) ...
-
bioRxiv - Microbiology 2024Quote: ... The total RNA was used in generation of cDNA using a reverse transcription kit (Bio-Rad, Hercules, CA) with gene-specific anti-sense primers ...
-
mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 8 µl sample lysate ...
-
bioRxiv - Cancer Biology 2021Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). Q-PCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein quantification was assessed using the BCA Protein Assay Kit (Pierce) or the RC DC Protein Assay (Bio-Rad).
-
bioRxiv - Molecular Biology 2020Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). qPCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
-
bioRxiv - Cell Biology 2020Quote: ... Molarity of the pool was quantified by qPCR using KAPA Library Quantification Kit and the CFX96 qPCR system (Biorad) before sequencing ...
-
bioRxiv - Cell Biology 2020Quote: ... Supernatant were transferred to fresh tube and solubilized protein was measured using a DC protein assay kit (Bio-Rad). For immunoblotting an appropriate volume of 1 x Laemmli (Bio-rad ...
-
bioRxiv - Cell Biology 2020Quote: ... Synthesis of complementary DNA (cDNA) was completed using 1 μg of RNA and the iScript cDNA synthesis kit (BioRad). Each qRT-PCR was performed as triplicate reactions with the following reaction mixture ...
-
bioRxiv - Immunology 2021Quote: ... Approximately 400 pg of RNA was subjected to reverse transcription using the iScript RT kit (BioRad, Inc., Hercules, CA). Resulting cDNA products were included with 50nM VH1-VH6 specific primers and 250nM Ca ...
-
bioRxiv - Microbiology 2021Quote: ... and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat. # 1708890, Bio-Rad). Real-time qPCR was conducted using the iTaq™ Universal SYBR® GreenSupermix (Cat ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 0.5 to 1μg RNA using SensiFast cDNA synthesis kit and qRT-PCR was performed using iTaq Universal SYBR Green Supermix (Biorad) (refer Purushothaman et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNAs were extracted by the Aurum ™ Total RNA mini kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subject to qRT-PCR assays by the Power SYBR® Green RNA-to-Ct™ 1-Step Kit (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2020Quote: ... and cDNA synthesis was carried out using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA Cat# 1708890) according to the manufacturers’ instructions.
-
bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 500 ng of total RNA was reverse transcribed with random primers using an iScript gClear cDNA synthesis kit (BioRad) and including a no-RT and controls for each sample ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNAs was prepared using RevertAid First Strand cDNA Synthesis Kit (Thermoscientific) according to the manufacturer’s recommendation to perform qRT-PCR (BioRad) (Sup Table 1) ...
-
bioRxiv - Cell Biology 2019Quote: ... Cell lysates were quantified for protein concentrations using the Bio-Rad DC™ Protein Assay kit (Cat.5000112, BioRad) and loaded to a 10% SDS-PAGE gel ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The detection of the proteins on the membrane was performed using the Clarity Western ECL Substrate kit (Bio-rad). The signals were captured using the Alliance Mini (UVITEC Cambridge ...
-
bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from REF subclones 2c and 11b using the Aurum Total RNA Mini Kit (Bio-rad) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... The reverse transcription is carried out with 0.5 μg of RNA with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the DC Protein assay Kit (Bio-Rad). 25 µg of the lysates were then separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
-
bioRxiv - Molecular Biology 2019Quote: ... Total RNA was then extracted using the Aurum Total RNA Mini Kit for Fatty and Fibrous Tissue (Bio-Rad). Reverse transcription reactions were performed using the iScript cDNA Synthesis Kit (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: ... Reverse Transcription cDNA synthesis reactions were performed on 0.2 μg −2 μg total RNA with iScript cDNA synthesis kit (BioRad) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using the Bradford method with the Quick Start™ Bradford Protein Assay Kit (#5000202, BIORAD) according to the manufacturer’s instruction34 ...
-
bioRxiv - Microbiology 2019Quote: ... each gene segment was PCR amplified (100μl total reaction) using a gene specific primer set with iProof High Fidelity PCR kit (BioRad), as per manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized by reverse transcription from isolated RNA using the iScript cDNA synthesis kit (Bio-Rad, 170–8891). Each 20 µl reaction followed the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was done using random primers for circRNA experiments from iScript™ Select cDNA Synthesis Kit (Bio-Rad). Fast Start Universal SYBR Green Master Mix (Roche ...
-
bioRxiv - Biochemistry 2021Quote: ... and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad, 170-4270). After blocking with 5 % fat-free milk in PBS-Tween 20 ...
-
bioRxiv - Genetics 2021Quote: ... Chemiluminescence detection was performed according to the manufacturer’s instructions (Amersham ECL Western Blotting Detection Reagent kit) followed by exposure using Chemidoc gel-documentation system (BioRad). For imaging using Li-Cor ...
-
bioRxiv - Genetics 2021Quote: ... normalized to 10ng/μL and then reverse transcribed into cDNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The cDNA was used as template for the droplet digital PCR (ddPCR ...
-
bioRxiv - Cancer Biology 2021Quote: ... Signals were developed using Amersham ECL Western Blotting Detection Kit (Cytiva, # RPN2108) and imaged on a Chemidoc Touch (Biorad). Relative protein quantitation was calculated based on normalized integrated intensity.
-
bioRxiv - Immunology 2021Quote: RNA was isolated using QIAGEN RNeasy Mini Kit and cDNA was synthesized using the iScript Reverse Transcription Supermix (Biorad). Quantitative real-time (qRT-PCR ...
-
bioRxiv - Microbiology 2021Quote: ... the samples were transferred onto a membrane using Trans-Blot® TurboTM RTA transfer system and kit (Bio-Rad). The membranes were saturated with 5% skimmed milk ...
-
bioRxiv - Cell Biology 2021Quote: ... RNA (1 μg) was reverse transcribed into cDNA using iScript Reverse Transcription Supermix Kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared to the control gene 18S were determined by quantitative real-time PCR using SYBER Green PCR Supermix (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 ug) was converted to cDNA using the iScriptTM cDNA synthesis kit (# 1708891, BIO-RAD, Hercules, CA) following the manufacturer’s protocol (BIO-RAD ...
-
bioRxiv - Bioengineering 2021Quote: ... The total RNA was then used to synthesize 200 ng of cDNA using the iScript Synthesis Kit (Biorad, #1708890) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... The cells were then used to isolate the total RNA using the Aurum Total RNA Mini Kit (Biorad, #7326820) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... 1μg of RNA for each sample was used to make cDNA using the iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... cDNA was synthesized using equal quantities of RNA (200 ng) with iScript Reverse Transcription kit (Bio-Rad Laboratories®).
-
bioRxiv - Neuroscience 2020Quote: ... 500 ng of RNA was retrotranscribed to single-stranded cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891). The analyses were performed in 3 animals/group ...
-
bioRxiv - Cell Biology 2020Quote: ... and quantified by real-time PCR using the IQ SYBR green supermix kit (Bio-Rad Laboratories, Hercules, CA, USA) and the following primers ...