Labshake search
Citations for Bio-Rad :
3501 - 3550 of 4986 citations for Olfactomedin 4 OLFM4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
-
bioRxiv - Cancer Biology 2023Quote: Protein samples were electrophoresed on home-made 4-16% Tris-glycine SDS-polyacrylamide gels and transferred to nitrocellulose membranes (0.45 µm, Bio-Rad, 1620115). Membranes were blocked in 5% non-fat milk ...
-
bioRxiv - Biophysics 2023Quote: ... Samples were heated at 95 °C for 5 minutes to denature and reduce the proteins and were then separated by electrophoresis through 4-15% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) with Tris/Glycine/SDS running buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 94°C before loading on polyacrylamide gels (4-15% Bis-Tris Gel, Bio-Rad). Proteins were separated by gel electrophoreses at 80 V and transferred at 90 V for 2 h on nitrocellulose membranes (Precast Protein Gels ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 95 ℃ for 10 minutes and resolved by SDS-PAGE using 4-20 % or 10 % precast gels (BioRad, 4561093), depending on the molecular weight of the desired protein ...
-
bioRxiv - Cell Biology 2023Quote: ... The denatured protein samples (20 µg) were then separated on 4-20 % SDS-polyacrylamide gels using a Tris-glycine running buffer (Bio-Rad) and electroblotted onto nitrocellulose membranes (0.2 µm pore size ...
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein was denatured at 95°C for 5 min in 1x Laemmli buffer containing 5% β-mercaptoethanol and separated in 4-15% mini-PROTEAN TGX Precast Protein Gels (Biorad) by electrophoresis and blotted onto Nitrocellulose membrane (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were subjected to electrophoresis under reducing conditions on 4–15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and subsequently transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were then clarified through centrifugation (14′000g, 20 min, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... For each protein of interest 10 µg of sample was loaded in a 4-15% Tris-Glycine gel (Bio-Rad, 4568083). The gel was transfered onto PVDF membrane (GenScript ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 µl of lysate was separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), and transferred to a PVDF membrane for analysis using α-FLAG antibody (Sigma Aldrich #F3165 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 4% SDS and 0.005% Bromophenol Blue) for 5min prior to loading on a 4%-20% Mini-PROTEAN TGX gels (BioRad, #456-1094) and blotted using Trans-Blot Turbo Transfer Pack 0.2μm nitrocellulose (BioRad ...
-
bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: RT-qPCR was employed to find the levels of quantification of mucin 1 and 4 mRNA transcripts according to protocol of the Real-Time PCR system (BioRad./ USA)[39] ...
-
bioRxiv - Microbiology 2023Quote: ... The protein samples were separated in 4 −15% acrylamide gels by SDS-PAGE using a Bio-Rad Mini-Protean Tera cell system (Bio-Rad). At the same time ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
-
bioRxiv - Biochemistry 2023Quote: 15µg was loaded on a 4-20% acrylamide gradient gel with 18 or 26-wells (Bio-Rad #5671094 & Bio-Rad #5671095) and separated for 20min at 100v followed by 150V for 1 hour ...
-
bioRxiv - Bioengineering 2024Quote: ... SDS-PAGE was performed using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (#4561084, Bio-Rad Laboratories, Inc, USA). Before and after the laser exposure ...
-
Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... and heated at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE for 4 minutes (or until all the sample had entered the gel) at 200V (two wells per sample; 4-20% Mini-PROTEAN TGX precast protein gel, BIO-RAD), then stained with Coomassie blue for ten minutes before being washed 4 X with ddH2O (10 minutes each ...
-
bioRxiv - Genetics 2024Quote: ... cDNA was diluted 1:4 with water and 2ul used as template for qPCR using 250nM primers using the SsoFast EvaGreen Supermix (BioRad 1725200) on an Applied Biosystems ViiA 7 Real Time PCR System ...
-
bioRxiv - Neuroscience 2024Quote: ... at 1-4 °C and 100 volts for 1 h using Criterion™ blotter with wire electrodes (BioRad, Cat#1704071, USA). Blots were rinsed with phosphate-buffered saline (PBS) ...
-
bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
-
bioRxiv - Neuroscience 2024Quote: 20-40 µg of denatured lysates were resolved by SDS-PAGE on 4-20% TGX Criterion TGX pre-cast gels (Bio-rad) and transferred to nitrocellulose membranes by semi-dry trans-Blot Turbo transfer system (Bio-rad) ...
-
bioRxiv - Bioengineering 2024Quote: The outcome of the reaction was analysed using native PAGE (4-20% Mini- PROTEAN TGX Precast Protein Gels, Bio-Rad, 4561096). 1 µL of the retic lysate reaction was diluted with 2 µL 1×PBS and mixed with 2× sample buffer containing 62.5 mM Tris ...
-
bioRxiv - Immunology 2024Quote: ... were washed with 500 μl ice-cold acetone to remove TCA at 16,000g for 5 min at 4 °C and resuspended in 100 μl 1x XT sample buffer (Bio-Rad #1610791), boiled at 95 °C for 10 min ...
-
bioRxiv - Biochemistry 2024Quote: Total proteins were visualized by in-gel fluorescence using a trihalo compound incorporated in the commercial pre-cast SDS–PAGE gels (stain-free TGX gels, 4–20%; Bio-Rad) after 45 sec UV-induced photoactivation using a ChemiDoc MP imager (Bio-Rad) ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for 5-10 minutes and then separated using SDS-PAGE with 4-12% Criterion XT Bis-Tris Gels (Bio-Rad). The proteins were transferred to PVDF membranes (Merck Millipore) ...
-
bioRxiv - Neuroscience 2024Quote: ... with DDT was added to 15µg of each sample and loaded into a 4–20% precast polyacrylamide gel (Bio-Rad, 4561095). The gel was electrophoresed for 10 minutes at 50V then 1 hour at 120V in Tris-Glycine SDS buffer (Bio-rad) ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were denatured for 10 min at 95°C and then 10 µl were loaded onto a precast 4-15% protein gel (Bio-Rad). Electrophoresis was performed by applying a constant voltage of 200 V in SDS running buffer (Tris/Glycine/SDS ...
-
bioRxiv - Immunology 2024Quote: ... 15μg of protein was loaded for each sample in precast 4-15% Mini-Protean TGX Stain-Free 30μl 10-well polyacrylamide gels (Bio-Rad #456803) along with Kaleidoscop protein ladder (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... heated at 95°C for 10 min and run on a Mini-protean TGX 4%-20% polyacrylamide gel (Biorad, 456-1095). The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
-
bioRxiv - Microbiology 2024Quote: Samples were resolved either on Mini-PROTEAN TGX Stain-Free Precast Gels or Mini-PROTEAN TGX Gels (4-20%) (Biorad, USA) in running buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
-
bioRxiv - Biochemistry 2024Quote: ... iSAT reactions started after addition of plasmid encoding rrnB under T7 promoter at 4 nM and the reaction was immediately incubated on pre-warmed CFX Connect Real-time System (Bio-Rad) at 37 °C for 90 mins in 96 well plates (Applied Biosystems) ...
-
bioRxiv - Microbiology 2024Quote: ... Each well was then blocked with 150 µL of blocking buffer consisting of 1×PBS + 4% (w/v) nonfat milk (Bio-Rad). The plates were blocked for 1 h at room temperature and then washed five times with PBST ...
-
bioRxiv - Developmental Biology 2024Quote: ... SDS-PAGE of protein extracts was performed with a BioRad Mini-Protean gel system using Mini-PROTEAN TGX Precast 4-15% polyacrylamide gels (BioRad 4561083) and blotted to Biorad Immun-Blot LF PVDF membrane (BioRad 1620174) ...
-
bioRxiv - Cell Biology 2024Quote: ... clarified at 20,000 g for 2 minutes and 15 µL was loaded onto a 4-20% tris-glycine SDS-PAGE gel (Biorad #5671094). Proteins were then transferred to nitrocellulose (Sigma #10600001 ...
-
bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
-
bioRxiv - Neuroscience 2024Quote: ... Oligomer formation was induced by incubating the peptide solution for 24 h at 4 °C and confirmed by polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining.
-
bioRxiv - Neuroscience 2024Quote: ... The membranes were then washed 4 times with PBST and then incubated in the dark for 5 min with Clarity Western ECL substrate (1705060, Bio-Rad), and imaged using the Amersham Imager 600 (GE Industries ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 μL of whole tissue lysate preps) were applied to 4−15% SDS-polyacrylamide gels (Bio-Rad; Hercules, CA, USA) and subjected to electrophoresis at 180 volts for 50 minutes in a preformulated 1× SDS-PAGE buffer (VWR) ...
-
bioRxiv - Immunology 2024Quote: ... 10 µL of each protein sample was then loaded and run on precast protein gels (4-20% Mini-PROTEAN® TGXTX, BioRad) before staining with Coomassie blue dye ...
-
bioRxiv - Cancer Biology 2021Quote: ... Secondary HRP-conjugated antibodies targeting mouse and rabbit IgG were purchased from Biorad.
-
bioRxiv - Developmental Biology 2020Quote: ... followed by horseradish peroxidise-conjugated secondary antibodies anti-rabbit (Bio-Rad 170–6515), anti-mouse (Bio-Rad 170–6516) ...
-
bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were goat anti-rabbit HRP (1:5000, Bio-Rad: 170-5046) and goat anti-mouse HRP (1:5000 ...
-
bioRxiv - Plant Biology 2019Quote: ... A secondary goat anti-rabbit horseradish peroxidase antibody (Bio-Rad Laboratories, Hercules, CA) was used at a 1:2,500 dilution For Supplemental Figure S4 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... A Fluorescein-conjugated goat anti-mouse was used as the second antibody (Biorad). Viral titers were 16,266±50,446 FFU and 155±125 FFU for CHIKV at 14 dpi and DENV at 21 dpi ...