Labshake search
Citations for Bio-Rad :
3751 - 3800 of 4986 citations for Olfactomedin 4 OLFM4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Proteins were separated by adding 20 μg of protein lysate to 4-20% gradient Mini Protean TGX Stain Free Pre-Cast Gels (Bio-Rad Laboratories). Proteins were then transferred to a nitrocellulose membrane (Cytiva ...
-
bioRxiv - Cell Biology 2024Quote: 40 μg of protein lysate was resolved on NuPAGE Novex 4-12 % Bis-Tris gels and transferred onto nitrocellulose membranes (Bio-Rad system). Membranes were blocked with 5 % BSA in TBS-T (10 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
-
bioRxiv - Genomics 2023Quote: ... The nuclei were isolated from cell homogenate by centrifugation and counted on an automated cell counter (TC20 BioRad, range 4-6 um). Transposition of 100k (weeks 1 ...
-
bioRxiv - Immunology 2024Quote: ... Lysates were separated by NuPAGE 4- 12 % Bis-Tris Gels (Thermo Fischer Scientific) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer Systems (Bio-Rad Laboratories). Membranes were blocked with Odyssey Blocking Buffer (Li-Cor ...
-
bioRxiv - Biochemistry 2023Quote: ... Boiled lysates were loaded on a 4-15% Tris-glycine gradient gel and run at 150 V using Tris/Glycine/SDS buffer (Bio-Rad, 1610732) until loading buffer reached the bottom of the gel ...
-
bioRxiv - Biochemistry 2023Quote: ... Histone samples (4 μg protein/well) or whole cell lysates (see above, 40 μg protein/well) were separated on precast gels (4-20%, BioRad, CA, USA) and transferred to 0.2 μm nitrocellulose membranes (Turbo transfer pack ...
-
bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
-
bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was performed on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories AG, Cat#4561094/4561096). Proteins were transferred to Trans-Blot Turbo 0.2 µm Nitrocellulose Transfer Membrane (Bio-Rad ...
-
bioRxiv - Biochemistry 2024Quote: ... The presence of the complex was assessed by 4-15% gradient SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein gels (BIORAD). Concentration of the RNAP in these fractions was determined by absorbance at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermofisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... Ten microliters of each soluble or chromatin-bound nuclear fraction were loaded on 4-20% CRITERION TGX SDS-PAGE gels (Bio-Rad, #4561085).
-
bioRxiv - Cell Biology 2024Quote: ... and 10 µg were then loaded and separated on precast 4–20% polyacrylamide Mini-PROTEAN TGX gels (Bio-Rad Laboratories, Hercules, CA). The proteins were then transferred to PVDF membranes (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of cell lysates were measured using BCA assay (Thermo 23225) and equal amounts of proteins were loaded onto 4 – 15 % polyacrylamide gradient gels (Bio-Rad 4561084) and fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: Equal amounts (10 μL) of the eluted solution from the streptavidin pulldown were loaded in a 4-20% polyacrylamide gel (Bio-RAD 4561096) and separated with SDS-PAGE at 100 V for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... A normalized volume equivalent to 20 μg total protein in the predigested sample was resolved on a 4-12% Criterion XT Bis-Tris gel (Bio-Rad 3450124) at 100V for 2 hours ...
-
bioRxiv - Immunology 2023Quote: ... A total of 30 µg of protein per well was loaded onto a 4-15% precast SDS-PAGE gel (Bio-Rad, 4561083). SDS-PAGE running conditions are as follows ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Samples were prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 90 °C for 10 min ...
-
bioRxiv - Microbiology 2023Quote: ... Protein samples were fractionated by SDS-polyacrylamide gel electrophoresis using a 4%–20% Mini-PRO TEAN TGX Gel (BioRad, Hercules CA, USA), and the resolved proteins were transferred by electroblotting onto polyvinylidene difluoride membranes (Immobilon-P PVDF Transfer Membranes) ...
-
bioRxiv - Neuroscience 2022Quote: ... extracted proteins were first immobilized and resolved based on molecular weight on 4–15% Mini-PROTEAN TGX Precast gels (Bio-Rad Laboratories) through sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) ...
-
bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein from whole cell lysates and conditioned media were analyzed by running samples on SDS/polyacrylamide gel electrophoresis (4-15% Stain-free SDS-PAGE minigels) and transferred to PVDF membrane (Immun-Blot PVDF, Bio-Rad 1620177). Membranes were blocked in 3% BSA in Tris-buffered saline containing 1% Tween-20 (pH7.4) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Samples are prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 70 °C for 3 min ...
-
bioRxiv - Neuroscience 2023Quote: ... were loaded onto 4%–12% sodium dodecyl sulfate–polyacrylamide gels (Absin, China) for 40 min using an electrophoresis apparatus (Bio-Rad, USA) and transferred to polyvinylidene difluoride membranes (Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... Laemmli buffer was added to all fractions before boiling the samples and loading them onto a 4-12% Bis-Tris Criterion™ acrylamide gel (Bio-Rad) and run in MOPS buffer (50 mM MOPS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Ten microliters of sample were pipetted onto SDS gels (4%–15% Criterion TGX Stain-free gels; Bio-Rad Laboratories; Hercules, CA, USA), and proteins were separated by electrophoresis (200 V for approximately 40 minutes) ...
-
bioRxiv - Biochemistry 2023Quote: ... different amounts of TTR1st in the range 0.1 1−1.10 μg were loaded in a native 4-15% PAGE gel (Mini-PROTEAN® TGX™ Precast Gel, Bio-Rad). The gel contained colored molecular weight standards (Precision Plus Protein Dual Color Standards ...
-
bioRxiv - Developmental Biology 2023Quote: 30 to 60 µg of protein obtained from AT2 were separated on 4-20% Criterion TGX Gels (Bio-Rad. Cat. No. 5678093), transferred to PVDF membranes (Trans-Blot Turbo System ...
-
bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
-
DNA Recognition and Induced Genome Modification by a Hydroxymethyl-γ Tail-Clamp Peptide Nucleic AcidbioRxiv - Genetics 2023Quote: ... Droplets were allowed to rest at 4 °C for at least 30 min after cycling and were then read using the QX200 ™ Droplet Reader (Bio-Rad). Data were analyzed using QuantaSoft™ software ...
-
bioRxiv - Cancer Biology 2023Quote: ... Between 40mg and 120mg of each protein sample was boiled in Laemmli buffer with 10% β-Mercaptoethanol and equal percentages of each sample were run on 4-15% polyacrylamide gels (BioRad, 4561086). Resolved proteins were wet-transferred to nitrocellulose membranes (Amersham) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... prestained protein ladder were applied to SDS-PAGE using 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad, Hercules, CA, USA). Western blots were carried out in a Trans-Blot Turbo system using Trans-Blot Turbo Mini 0.2 μm Transfer-Packs (Bio-Rad ...
-
bioRxiv - Biochemistry 2023Quote: ... and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678084) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1 hour at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA was diluted to a 500 ng/16 μl concentration using water and combined with 4 μl of iScript Reverse Transcription Supermix for RT-qPCR (Biorad, Cat# 1708841) and processed in a thermocycler (MJ Research PTC-200 Peltier Thermal Cycler ...
-
bioRxiv - Molecular Biology 2023Quote: ... the sample boiled for five minutes at 95 °C and size-separated by SDS-PAGE using a 4–20% Mini-PROTEAN® TGX™ Gel (Biorad). Proteins were transferred onto a nitrocellulose membrane (Biorad ...
-
bioRxiv - Microbiology 2023Quote: Volumes of lysates corresponding to 20 μg of protein were then run on a Criterion XT 4- 12% Bis-Tris Precast Gel (Bio-Rad, 3450124) in MES running buffer (50 mM MES ...
-
bioRxiv - Molecular Biology 2023Quote: ... containing 25 μg of each plasmid construct and 25 μg of donor DNA in 4-mm electroporation cuvettes with three pulses (1500 volts, 25 microfarads) delivered by a Gene Pulser Xcell™ Electroporation System (Bio-Rad). Transfected epimastigotes were cultured in LIT medium supplemented with 20% heat-inactivated FBS until stable cell lines were obtained ...
-
bioRxiv - Bioengineering 2022Quote: ... Fifteen micrograms total protein extracts were separated by 4–20 % stain-Free SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories). Membranes were blocked using Tris-buffered saline (TBS ...
-
bioRxiv - Biochemistry 2022Quote: ... The same volume of all samples (corresponding to ∼15 µg starting FBS dilution) was separated by SDS-PAGE using 4-20% polyacrylamide Mini-Protean® TGXTM Gels (#456-1094, Bio-Rad) and wet-transferred to Nitrocellulose membranes (#1620112 ...
-
bioRxiv - Biochemistry 2022Quote: ... Gel demonstrating protein purity was generated using by loading 1 μg of each protein onto a Mini-PROTEAN TGX 4-20% gradient gel (BIO-RAD, 4561096) and then staining with Coomassie blue.
-
bioRxiv - Cancer Biology 2023Quote: ... All samples were mixed with loading buffer containing SDS and β-mercaptoethanol and run on homemade 15% or commercial 4-15% gradient acrylamide gels (Bio-Rad). Semi-dry transfer was performed on a Trans-Blot Turbo transfer system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were boiled at 100 °C for 5 min and loaded in handcrafted 8% SDS-polyacrylamide gel or 4-12% Criterion™ XT Bis-Tris XT Precast Gels (Bio-Rad) and electrophoresed in 1X Tris/Glycine/SDS buffer (Bio-Rad ...
-
bioRxiv - Biophysics 2023Quote: ... The mixture was incubated for 1-2 h at 4 °C and incubated overnight with pre-washed bio-beads (Bio-Rad Laboratories) at 4 °C ...
-
bioRxiv - Biophysics 2023Quote: ... an aliquot of 25µg of total protein per sample was loaded into 4–20% Mini-PROTEAN® TGX™ precast gels (Bio-Rad) for SDS-PAGE separation ...
-
bioRxiv - Genetics 2023Quote: ... 50 µg of whole protein lysate was used for all immunoblots and resolved on 4-20% Mini-PROTEAN TGX Precast Protein gels (BioRad; Cat# 4561094). Protein samples were transferred to 0.2 µm PDVF membranes (ThermoFisher ...
-
bioRxiv - Genetics 2023Quote: ... and equal amounts of protein sample were then loaded onto a 4-20% gradient SDS-PAGE gel (Bio-Rad, cat no. 4561095) and run at 120V before being transferred to a PVDF membrane (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and 10 µl of protein sample was loaded in each well of precast Mini-PROTEAN TGX 4-20% gradient Gel (BIO-RAD 4561096). Following SDS-PAGE ...
-
bioRxiv - Bioengineering 2023Quote: ... 15 ug protein were loaded and separated using 4–15% Mini-PROTEAN® TGX™ Precast SDS-PAGE Gels (Bio-rad, #4561086). Samples were then transferred to nitrocellulose membranes for immunoblotting ...
-
bioRxiv - Immunology 2023Quote: ... and cell pellets were lysed in 1× Laemmli buffer before proteins were separated on 4-20% Mini- PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and transferred to a nitrocellulose membrane (Odyssey® nitrocellulose membrane ...
-
bioRxiv - Neuroscience 2023Quote: ... were incubated in a boiling bath for 10 min and analyzed by SDS-PAGE on 4–20% precast polyacrylamide gradient gel (Bio-Rad, 4561096). Western blot analyses were performed according to standard protocols using antibodies against MBP (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... the samples were loaded on a 10% SDS-PAGE gel made in-lab or on a 4-15% pre-cast TGX gel (Bio-Rad, #4568086), along with the molecular marker ...