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Citations for Bio-Rad :
3501 - 3550 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Maltose-1-phosphate was separated from orthophosphate by anion exchange chromatography using Dowex 1 × 8 100-200 mesh (Bio-Rad, 28 x 1.6 cm ...
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bioRxiv - Cell Biology 2022Quote: ... Corresponding mouse or rabbit horseradish peroxidase-conjugated secondary antibodies (diluted 1:10,000 in 1x TBST) were added for 1 hr at room temperature (Bio-Rad). Chemiluminescence was detected by adding Clarity Western ECL Substrate (1:1 of luminol and peroxide solutions ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for immunoblotting: Talin-1 (1:5000 dilution, Mouse monoclonal, Clone 97H6; Bio-Rad, Cat# MCA4770), YAP (1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 hour at RT with 1:10,000 goat anti-rabbit horseradish peroxidase (HRP) (Bio-Rad, Cat# STAR208P). The membrane was washed 3 times for 5 minutes with TBST ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and antimouse secondary antibody (Biorad; 1:10,000). GroEL was used as cytoplasmic control and detected as above ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies (Anti-Rabbit or Anti-Mouse, CST, 1:5000 and 1:2000) and revealed using Clarity Western ECL (BioRad). Image J was used to quantify the intensity of western blot bands ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in 0.1% TBS-Tween and incubated with the secondary antibody (1:10000) for 1 h at room temperature (RT) and revealed by chemiluminescence (Clarity ECL western blotting substrates, BioRad) using ImageQuant LAS 500 chemiluminescence CCD camera (GE Healthcare Bioscience AB) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Biochemistry 2023Quote: ... expression media samples were diluted with 2X reducing SDS sample buffer at 1:1 ratio and the mixture was loaded on a 10-well 4–20% SDS-PAGE gradient gel (BioRad). Image J software was used to quantify the signal compared to the negative control (mock transfected culture) ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies and dilutions were used for 1 hour at room temperature: goat anti-rabbit StarBright Blue 700 (1:2,500; Bio-Rad), goat anti-rabbit IRDye 800 CW (1:5,000 ...
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bioRxiv - Microbiology 2024Quote: ... The dried glycolipid fraction was redissolved in 1 mL of CHCl3/CH3OH (19:1) and applied to a column of silica gel (BioRad) equilibrated in CHCl3 ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was performed using 3.5 μl of diluted cDNA and 5.5 μM primers listed in Table 2 (C.-M. Lee and Thomashow, 2012; Wu et al., 2008)using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, cat# 1725121) with the CFX 384 TouchTM Real-Time PCR Detection System (Bio-RAD) ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... This mix was transferred to a pre-chilled 2 mm electroporation cuvette and electroporation was performed (2.0 kV, 25 μF, 200 Ω) using a Gene Pulser Xcell (Bio-Rad Laboratories, Hercules, CA) electroporation sys-tem ...
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bioRxiv - Biochemistry 2019Quote: ... Three hexasaccharides with unsaturated uronic acid at the non-reducing end were chemically synthesized using modified reported procedures and the reaction products were desalted over a Bio-Gel P-2 column (Bio-Rad, Hercules, CA, USA) without further purification ...
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Genetics 2022Quote: ... of each mating type of the strains of interest were placed in 2 mL tubes containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Cell Biology 2019Quote: ... Separation in the second dimension was accomplished using an 10% SDS-PAGE in a Protean II xi 2-D Cell (Bio-Rad, Hercules, CA, USA) at 50 mA until the bromophenol blue reached the bottom of the gel ...
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bioRxiv - Synthetic Biology 2020Quote: The constructs were transformed into Agrobacterium tumefaciens strain pGV3850 by electroporation (2 mm cuvette, 2.5 kV, 400 Ω, and 25 μF) in a Bio-Rad GenePulser (Bio-Rad, Hercules, CA, USA). Cells were incubated at 28°C for 2 h after 200 μl LB media was added ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... Solid phase specificity was confirmed with dot blots of serial antigen dilutions (2 μL) applied to nitrocellulose membrane (Bio-Rad 0.45 µm; Fig. 1b). Membranes were allowed to air dry (1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were performed in total 24ul volume using 12ul of 2 x ddPCR Supermix for Probes (No dUTP) (Bio-Rad Laboratories, catalog no. 1863024), 900nM of target primer pairs ...
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bioRxiv - Biochemistry 2022Quote: ... We mixed 10 μL of each fraction with 2 μL of denaturing RNA loading dye and loaded it into a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Urea-PAGE (7.5% ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... DNA was diluted to 10 ng/μl and 2 μl were used in each RT-qPCR reaction with 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) and 400 nM of forward and reverse primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg of dsDNA were added to the competent cells and then transferred to chilled 2 mm gap electroporation cuvettes (Bio-Rad Laboratories GmbH, Germany). Electroporation transformation was done with a single pulse at 1.8 kV ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of total RNA was reverse-transcribed to single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad Hercules, CA, USA). RT-qPCR was run on Lightcycler 480 (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... under reducing conditions using 2-ME and transferred onto polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo system (Bio-Rad, Hercules, CA, USA). After blocking with 10% skim milk (Becton Dickinson ...
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bioRxiv - Molecular Biology 2020Quote: ... sheep polyclonal to TGN46 (Biorad #AHP500G, 1:1000), rat monoclonal to myc-tag (Chromotek #9e1 ...
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bioRxiv - Developmental Biology 2021Quote: ... V5 (Bio-Rad: DyLight®550, 1:800), rat anti-DN-cadherin (DSHB ...
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bioRxiv - Cell Biology 2020Quote: ... packed with AGMP-1 resin (Bio-Rad Laboratories). Samples (100 μl ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1% β-mercaptoethanol (Bio-Rad, 1610710) and RNA was isolated using the RNeasy Mini Kit (Qiagen 74104 ...
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bioRxiv - Microbiology 2020Quote: ... and permeabilized with 1% Triton X-100 (BioRad) in PBS for 10 min ...
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bioRxiv - Systems Biology 2022Quote: ... rat anti-F4/80 (BioRad, MCA497R, 1:100) or rabbit anti-CYP2E1 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5 mg of 1 μm-gold microparticles (Biorad) were coated with 15 μg GFP-Rab4 plasmid and slices were transfected making use of the DNA transformation apparatus ADNGun (Beambio) ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-Ube2N (Bio-Rad, AHP974, 1:1000), rabbit anti-IKKα (Y463 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-V5 (1:2,000, mouse, Biorad; western blotting), anti-PAP (1:20,000 ...
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bioRxiv - Immunology 2021Quote: ... or -CD68 (1:500, Bio-Rad, Catalog MCA1957) overnight at 4°C ...
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bioRxiv - Physiology 2019Quote: ... 1 cycle) on a C1000 thermal cycler (BioRad). At completion of end-point PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... and anti-F4/80 (1:1000, MCA497R, Biorad) were used to stain the lumen epithelium and macrophages ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated goat anti-rabbit (1:1,500, Biorad) secondary antibodies were used ...
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bioRxiv - Developmental Biology 2020Quote: ... V5 at 1:1000 (mouse, Bio-rad, #MCA1360) [23] ...
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bioRxiv - Molecular Biology 2019Quote: ... mouse monoclonal GAPDH (Bio-Rad, 1:1,000, VMA00046), rabbit polyclonal anti-GAPDH (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL casein (Blotting-Grade Blocker, Biorad), 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: sheep polyclonal anti-TGN46 (Biorad; 1:200, IFM); mouse monoclonal anti-β-actin [Sigma A5316 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-VAPB (VMA00454; PLA, 1:600) from BioRad, Anti-IP3R3 (07-1213 ...
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bioRxiv - Plant Biology 2020Quote: ... 1% agarose gel (Pulse Field Certified Agarose, BioRad) was prepared using 1X TBE buffer and casted in a PFGE casting stand ...
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bioRxiv - Plant Biology 2020Quote: ... 1 volume of Laemmli buffer (Bio-Rad, US) was added to 1 volume of extracted proteins (30 µg) ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit (1:3000; BioRad, Hercules, CA) antibodies ...
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bioRxiv - Immunology 2021Quote: ... F4/80 antibody | Cl:A3-1 #MCA497PBT (Bio-Rad); BV650 Hamster Anti-Mouse CD11c Clone HL3 (RUO ...